scholarly journals Prevalence and molecular characterization of Escherichia coli isolates during radish sprout production in the Republic of Korea

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Nagendran Rajalingam ◽  
Jae-Hyun Yoon ◽  
Bohyun Yoon ◽  
Nguyen Bao Hung ◽  
Won-Il Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stream Water ◽  
Summer Season ◽  
Republic Of Korea ◽  
Safety Measures ◽  
E Coli ◽  
Radish Sprouts ◽  
The Republic ◽  
Sequence Types

AbstractThis study was conducted to investigate the prevalence of coliforms and Escherichia coli across radish sprout production facilities in the Republic of Korea and to characterize isolated E. coli. A total of 324 samples were collected from three different radish sprout production units in spring and summer. Overall, the levels of coliforms were significantly higher (> 3.75 log CFU/g) in radish sprouts during summer season than in spring. E. coli was detected in the pre-harvest (50%) and post-harvest (39%) sprouts and water samples (33%) from farms B and C. Of the 48 E. coli isolates, five E. coli isolates were positive for the eaeA gene, indicative of EPEC O110, two isolates were eaeA-positive (EPEC ONT) and one isolate was ipaH-positive (ETEC O71). These three E. coli strains were identified as novel sequence types by the multilocus sequence-typing assay based on the combination of allelic profiles. In addition, the EPEC O110 isolates obtained from the stream water stored in container of farm C were toxic to Caenorhabditis elegans. It is necessary to implement safety measures during the production of radish sprouts to prevent E. coli outbreaks.

scholarly journals Molecular Characterization of Antimicrobial Resistance in Escherichia coli from Rabbit Farms in Tai’an, China

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Xiaonan Zhao ◽  
Jie Yang ◽  
Zijing Ju ◽  
Weishan Chang ◽  
Shuhong Sun
Keyword(s):  
Escherichia Coli ◽  
Gene Cassette ◽  
Human Medicine ◽  
Class I ◽  
E Coli ◽  
Faecal Samples ◽  
Class I Integrons ◽  
Reasonable Use ◽  
Sequence Types

To investigate the prevalence and resistance against antimicrobials of Escherichia coli (E. coli) in Tai’an, March 2016, a total of 55 E. coli strains were isolated from 60 faecal samples of diarrheic rabbits collected from three rabbit farms in Tai’an. The E. coli isolates were assayed for antimicrobial susceptibility and prevalence of resistance genes and Class I integrons and genotyped using Multilocus Sequence Typing (MLST). All the E. coli isolates were sensitive to ceftazidime, ceftriaxone, imipenem, and amikacin, while 78.2% of the isolates showed resistance against tetracycline, and 65.5% were resistant against ampicillin. The most common resistance gene detected was blaTEM, present in 98.2% of isolates, followed by blaCTX-M (94.6%) and sul2 (58.2%). Class I integrons were detected in 17 out of the 55 (30.9%) E. coli strains. Seven kinds of gene cassette were detected: dfrA17 + aadA5, dfrA1 + catB3 + aacA4, aadA2 + LinF, dfrA1 + aadA1, aadA22, dfrA12 + orfF + addA2, and aadA16 + dfrA27 + arr-3. All the 55 E. coli strains were identified and classified as 13 sequence types (STs); ST302 (22/55, 40.0%) was the most prevalent type, followed by ST370 (12/55, 21.8%). This study showed that E. coli isolated from diarrheic farmed rabbits in the Tai’an area exhibit sometimes very frequent resistance to antimicrobials important to human medicine, which further highlights the need for reasonable use of antibiotics.

scholarly journals Genetic Characterization of Plasmid-mediated qepA Gene Among ESBL-producing Escherichia Coli Isolates in Mexico

2021 ◽  
Author(s):  
Humberto Barrios-Camacho ◽  
Josefina Duran-Bedolla ◽  
Jesus Silva-Sanchez ◽  
Luis Lozano-Aguirre ◽  
Fernando Reyna-Flores ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
Genetic Characterization ◽  
Insertion Sequences ◽  
Plasmid Sequence ◽  
E Coli ◽  
Multiple Copies ◽  
Chromosomal Mutations ◽  
Sequence Types

Abstract A molecular characterization of a plasmid-born qepA gene in (ESBL)-producing E. coli clinical isolates were performed. An 2.63% (11/418) were qepA positive isolates, of which a 90.0% carried CTX-M-15 (9/11) and SHV-12 (1/11). All isolates showed chromosomal mutations in the gyrA and parC genes. The clonal groups A, B and C were identified and belonged to, respectively, phylogroups A, B1 and D, as well as the sequence types 205, 405 and 617. Several plasmid profiles were determined with incompatibility groups FIA, FIB and FII. The genetic environment of the qepA in plasmid pEC8020 was different from those reported previously. The plasmid sequence included genes conferring resistance to β-lactams (blaCTX-M-15), macrolides (mphA), fluoroquinolones (qepA1), trimethoprim (dfrB4) and sulphonamides (sul1). Likewise, the IncF-pEC8020 plasmid carried several insertion sequences including ISCR3, IS6100 and multiple copies of IS26. This work contributes to the epidemiology and genetics of plasmid-born qepA genes of ESBL-producing E. coli.

scholarly journals Genome Sequence of Bacteriophage GG32, Which Can Infect both Salmonella enterica Serovar Typhimurium and Escherichia coli O157:H7

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Su-Jin Chae ◽  
Taesoo Kwon ◽  
Sunjin Lee ◽  
Yeon Ho Kang ◽  
Gyung Tae Chung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Control Agent ◽  
Republic Of Korea ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Serovar Typhimurium ◽  
The Republic

We report here a new virulent Salmonella enterica serovar Typhimurium ( S . Typhimurium) bacteriophage, GG32, which was isolated from the Guem River in the Republic of Korea. The strain can infect both S . Typhimurium and Escherichia coli ( E. coli ) O157:H7 and may be a good candidate for a bio-control agent.

Characterization of Extended-Spectrum β-Lactamase–Producing Escherichia coli Isolates from Pigs and Farm Workers

2016 ◽  
Vol 79 (9) ◽  
pp. 1630-1634 ◽  
Author(s):  
HONGNA ZHANG ◽  
ZHENZHEN ZHAI ◽  
QING LI ◽  
LINGHONG LIU ◽  
SHUYUAN GUO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Farm Workers ◽  
Enterobacterial Repetitive Intergenic Consensus ◽  
E Coli ◽  
Extended Spectrum ◽  
Rectal Swabs ◽  
Pig Farm ◽  
Sequence Types

ABSTRACT Food-producing animals can serve as reservoirs for extended-spectrum β-lactamase (ESBL)–producing Escherichia coli. The present study aimed to characterize and compare ESBL-carrying E. coli isolates from both pigs and farm workers. Rectal swabs were obtained from 60 pigs on four pig-fattening farms (15 samples per farm), and rectal swabs were taken from 40 farm workers on these farms (10 samples per farm). ESBL-carrying E. coli isolates from the workers and pigs were characterized by ESBL genotype, antibiotic susceptibility, enterobacterial repetitive intergenic consensus type, and multilocus sequence type. ESBL-producing E. coli was detected in 34 (56.7%) of 60 pigs, and 20.0% (8 of 40) of the farm workers were positive for ESBL-producing E. coli. More importantly, ESBL-producing E. coli isolates with the same β-lactamase genes, antibiotic resistance profiles, enterobacterial repetitive intergenic consensus types, and multilocus sequence types were detected in both pigs and workers on the same pig farm. These findings were suggestive for transfer of ESBL-producing E. coli between animals and humans.

scholarly journals Molecular Characterization of Extended-Spectrum-β-Lactamase-Producing and Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Stray Dogs in South Korea

2012 ◽  
Vol 56 (5) ◽  
pp. 2705-2712 ◽  
Author(s):  
Migma Dorji Tamang ◽  
Hyang-Mi Nam ◽  
Geum-Chan Jang ◽  
Su-Ran Kim ◽  
Myung Hwa Chae ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Companion Animals ◽  
Republic Of Korea ◽  
Stray Dogs ◽  
Content Type ◽  
First Report ◽  
E Coli ◽  
Extended Spectrum ◽  
The Republic ◽  
Clonal Spread

ABSTRACTA total of 47 extended-spectrum-cephalosporin-resistantEscherichia colistrains isolated from stray dogs in 2006 and 2007 in the Republic of Korea were investigated using molecular methods. Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase phenotypes were identified in 12 and 23E. coliisolates, respectively. All 12 ESBL-producing isolates carriedblaCTX-Mgenes. The most common CTX-M types were CTX-M-14 (n= 5) and CTX-M-24 (n= 3). Isolates producing CTX-M-3, CTX-M-55, CTX-M-27, and CTX-M-65 were also identified. Twenty-one of 23 AmpC β-lactamase-producing isolates were found to carryblaCMY-2genes. TEM-1 was associated with CTX-M and CMY-2 β-lactamases in 4 and 15 isolates, respectively. In addition toblaTEM-1, two isolates carriedblaDHA-1, and one of them cocarriedblaCMY-2. Both CTX-M and CMY-2 genes were located on large (40 to 170 kb) conjugative plasmids that contained the insertion sequence ISEcp1upstream of theblagenes. Only in the case of CTX-M genes was there an IS903sequence downstream of the gene. The spread of ESBLs and AmpC β-lactamases occurred via both horizontal gene transfer, accounting for much of the CTX-M gene dissemination, and clonal spread, accounting for CMY-2 gene dissemination. The horizontal dissemination ofblaCTX-MandblaCMY-2genes was mediated by IncF and IncI1-Iγ plasmids, respectively. The clonal spread ofblaCMY-2was driven mainly byE. colistrains of virulent phylogroup D lineage ST648. To our knowledge, this is the first report ofblaDHA-1inE. colistrains isolated from companion animals. This study also represents the first report of CMY-2 β-lactamase-producingE. coliisolates from dogs in the Republic of Korea.

scholarly journals Molecular Characterization of CTX-M β-Lactamase and Associated Addiction Systems in Escherichia coli Circulating among Cattle, Farm Workers, and the Farm Environment

2013 ◽  
Vol 79 (13) ◽  
pp. 3898-3905 ◽  
Author(s):  
Migma Dorji Tamang ◽  
Hyang-Mi Nam ◽  
Mamata Gurung ◽  
Geum-Chan Jang ◽  
Su-Ran Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Farm Workers ◽  
Host Cells ◽  
Conjugative Plasmid ◽  
Republic Of Korea ◽  
Cattle Farm ◽  
Content Type ◽  
E Coli ◽  
The Republic ◽  
M 14

ABSTRACTA total of 84 extended-spectrum β-lactamase (ESBL)-producingEscherichia coliisolates from cattle, farm workers, and the farm environment isolated from February to September 2008 in the Republic of Korea were investigated. All 84 ESBL-producing isolates carriedblaCTX-Mgenes that belonged to the CTX-M-1 (n= 35) or CTX-M-9 (n= 49) family. The most predominant CTX-M type identified was CTX-M-14 (n= 49), followed by CTX-M-32 (n= 26). TheblaCTX-Mgenes were identified most commonly inE. coliisolates from feces (n= 29), teats (n= 25), and milk (n= 14). AblaCTX-M-14gene was also detected in anE. coliisolate from a farmer's hand. Transfer of theblaCTX-Mgene from 60blaCTX-M-positiveE. coliisolates to the recipientE. coliJ53 strain by conjugation was demonstrated. Plasmid isolation fromblaCTX-M-positive transconjugants revealed a large (95- to 140-kb) conjugative plasmid. Almost all (82/84)blaCTX-Mgenes possessed an insertion sequence, ISEcp1, upstream of theblaCTX-Mgene. Only in the case of the CTX-M-14 genes was IS903downstream of the gene. TheblaCTX-Mgenes were associated with seven kinds of addiction systems. Among them,pndAC,hok-sok, andsrnBCwere the most frequently identified addiction systems in both wild strains and transconjugants. The spread ofblaCTX-Mgenes was attributed to both clonal expansion and horizontal dissemination. Our data suggest that a combination of multiple addiction systems in plasmids carryingblaCTX-Mgenes could contribute to their maintenance in the host cells. To our knowledge, theblaCTX-M-32gene has not previously been reported in animal isolates from the Republic of Korea.

scholarly journals Presence of β-Lactamase-producing Enterobacterales and Salmonella Isolates in Marine Mammals

2021 ◽  
Vol 22 (11) ◽  
pp. 5905
Author(s):  
Olivia M. Grünzweil ◽  
Lauren Palmer ◽  
Adriana Cabal ◽  
Michael P. Szostak ◽  
Werner Ruppitsch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Marine Mammals ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Healthcare Sector ◽  
Whole Genome ◽  
E Coli ◽  
High Prevalence ◽  
Lactamase Gene ◽  
Sequence Types

Marine mammals have been described as sentinels of the health of marine ecosystems. Therefore, the aim of this study was to investigate (i) the presence of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Enterobacterales, which comprise several bacterial families important to the healthcare sector, as well as (ii) the presence of Salmonella in these coastal animals. The antimicrobial resistance pheno- and genotypes, as well as biocide susceptibility of Enterobacterales isolated from stranded marine mammals, were determined prior to their rehabilitation. All E. coli isolates (n = 27) were screened for virulence genes via DNA-based microarray, and twelve selected E. coli isolates were analyzed by whole-genome sequencing. Seventy-one percent of the Enterobacterales isolates exhibited a multidrug-resistant (MDR) pheno- and genotype. The gene blaCMY (n = 51) was the predominant β-lactamase gene. In addition, blaTEM-1 (n = 38), blaSHV-33 (n = 8), blaCTX-M-15 (n = 7), blaOXA-1 (n = 7), blaSHV-11 (n = 3), and blaDHA-1 (n = 2) were detected. The most prevalent non-β-lactamase genes were sul2 (n = 38), strA (n = 34), strB (n = 34), and tet(A) (n = 34). Escherichia coli isolates belonging to the pandemic sequence types (STs) ST38, ST167, and ST648 were identified. Among Salmonella isolates (n = 18), S. Havana was the most prevalent serotype. The present study revealed a high prevalence of MDR bacteria and the presence of pandemic high-risk clones, both of which are indicators of anthropogenic antimicrobial pollution, in marine mammals.

scholarly journals Molecular characterization of clinical carbapenem-resistant Enterobacterales from Qatar

2021 ◽  
Author(s):  
Fatma Ben Abid ◽  
Clement K. M. Tsui ◽  
Yohei Doi ◽  
Anand Deshmukh ◽  
Christi L. McElheny ◽  
...  
Keyword(s):  
Common Species ◽  
Clinical Samples ◽  
Whole Genome ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Genes Encoding ◽  
Encoding Genes ◽  
Sequence Types ◽  
Common Sequence

AbstractOne hundred forty-nine carbapenem-resistant Enterobacterales from clinical samples obtained between April 2014 and November 2017 were subjected to whole genome sequencing and multi-locus sequence typing. Klebsiella pneumoniae (81, 54.4%) and Escherichia coli (38, 25.5%) were the most common species. Genes encoding metallo-β-lactamases were detected in 68 (45.8%) isolates, and OXA-48-like enzymes in 60 (40.3%). blaNDM-1 (45; 30.2%) and blaOXA-48 (29; 19.5%) were the most frequent. KPC-encoding genes were identified in 5 (3.6%) isolates. Most common sequence types were E. coli ST410 (8; 21.1%) and ST38 (7; 18.4%), and K. pneumoniae ST147 (13; 16%) and ST231 (7; 8.6%).

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