scholarly journals Detection of thioredoxin-1 using ultra-sensitive ELISA with enzyme-encapsulated human serum albumin nanoparticle

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Myeong-Jun Lee ◽  
Eun-Sol Lee ◽  
Tae-Hwan Kim ◽  
Ju-Won Jeon ◽  
YongTae Kim ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Self Assembly ◽  
Sandwich Elisa ◽  
Protein A ◽  
High Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Synthesis Conditions ◽  
Thioredoxin 1

AbstractMany methods for early diagnosis of the disease use biomarker tests, which measure indicators of biological state in body fluids or blood. However, a limitation of these methods is their low sensitivity to biomarkers. In this study, human serum albumin (HSA) based nanoparticles capable of encapsulating excess horseradish peroxidase (HRP) are synthesized and applied to the development of enzyme-linked immunosorbent assay (ELISA) kit with ultra-high sensitivity. The size of the nanoparticles and the amount of encapsulated enzyme are controlled by varying the synthesis conditions of pH and protein concentration, and the surface of the nanoparticles is modified with protein A (proA) to immobilize antibodies to the nanoparticles by self-assembly. Using the synthesized nanoparticles, the biomarker of breast cancer, thioredoxin-1, can be measured in the range of 10 fM to 100 pM by direct sandwich ELISA, which is 105 times more sensitive than conventional methods.

Monitoring the self-assembly of chitosan/glutaraldehyde/cysteamine/Au-colloid and the binding of human serum albumin with hesperidin

Biomaterials ◽  
2004 ◽  
Vol 25 (26) ◽  
pp. 5725-5733 ◽  
Author(s):  
Yingju Liu ◽  
Yanling Li ◽  
Shanchao Liu ◽  
Jia Li ◽  
Shouzhuo Yao
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Self Assembly ◽  
The Self

Paclitaxel-induced formation of 3D nanocrystal superlattices within injectable protein-based hybrid nanoparticles

2018 ◽  
Vol 54 (82) ◽  
pp. 11586-11589
Author(s):  
Jeong Yu Lee ◽  
Ho Yeon Son ◽  
Jae Chul Park ◽  
Jongnam Park ◽  
Yoon Sung Nam
Keyword(s):  
Polyethylene Glycol ◽  
Iron Oxide ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Self Assembly ◽  
Three Dimensional ◽  
Superparamagnetic Iron Oxide ◽  
Hybrid Nanoparticles ◽  
Nanocrystal Superlattices

Self-assembly of monodisperse superparamagnetic iron oxide nanocrystals into a close-packed, three-dimensional (3D) superlattice is designed within cross-linked protein-based nanoparticles composed of human serum albumin and polyethylene glycol.

Microfluidic self-assembly of high cabazitaxel loading albumin nanoparticles

Nanoscale ◽  
2020 ◽  
Vol 12 (32) ◽  
pp. 16928-16933
Author(s):  
Yating Sun ◽  
Robert J. Lee ◽  
Fanchao Meng ◽  
Guiyuan Wang ◽  
Xiaolong Zheng ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Self Assembly ◽  
Promising Method ◽  
Albumin Nanoparticles ◽  
Microfluidic Technology

Microfluidic technology for the preparation of human serum albumin nanoparticles is a promising method.

Self-Assembled N-Succinyl-Chitosan Nanofibers for Reduced Protein Adhesion

2010 ◽  
Vol 76 ◽  
pp. 36-41
Author(s):  
Eyas Dayyoub ◽  
Udo Bakowsky
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Self Assembly ◽  
Adhesion Properties ◽  
Force Microscopy ◽  
Atomic Force ◽  
Biomaterial Surfaces ◽  
Force Curves ◽  
Protein Adhesion

Protein adhesion on biomaterial surfaces plays a major role in determining their biocompatibility and cell responses. The goal of this study was to produce chitosan-based coatings of implant material polyurethane (PUR) for reduced human serum albumin (HSA) adhesion. Succinic anhydride was employed for modifying chitosan and synthesis of N-succinyl-chitosan (NSCS) which was used as a matrix coating of PUR. NSCS showed self-assembly behaviour as nanofiber structures onto PUR surface. Atomic force microscopy (AFM) has emerged as useful tool for the molecular force measurements and therefore it has been chosen to investigate the adhesion properties of Human serum albumin (HSA) on the new matrix coatings and other three implant materials PUR, Silicon and Titanium. HSA molecules were covalently bound to the AFM tip by the use of cyanuric chloride as bivalent linker. Analyzing the force curves demonstrated the antiadhesive properties of the NSCS films in comparison with the uncoated PUR, Silicon and Titanium.

scholarly journals Analysis of aflatoxin B1-human serum albumin adducts by a sandwich enzyme-linked immunosorbent assay

1996 ◽  
Vol 1996 (43) ◽  
pp. 43-46 ◽  
Author(s):  
Osamu KAWAMURA ◽  
J.-M. LIM ◽  
Hiroki OKUMURA ◽  
Sigefumi KISHIMOTO ◽  
G. CHEN ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Estimation of Group B Streptococcus Type III Polysaccharide-Specific Antibody Concentrations in Human Sera Is Antigen Dependent

1998 ◽  
Vol 66 (12) ◽  
pp. 5848-5853 ◽  
Author(s):  
Reva Bhushan ◽  
Bascom F. Anthony ◽  
Carl E. Frasch
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Conjugate Vaccine ◽  
Group B Streptococcus ◽  
Antigenic Specificity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type Iii ◽  
Human Sera ◽  
Group B

ABSTRACT The presence of immunoglobulin G (IgG) antibodies against group B streptococcus (GBS) type III polysaccharide (PS) has been correlated with protection against GBS disease. The GBS type III PS is structurally similar to the pneumococcal type 14 PS, differing only in the presence of sialic acid residues. Four different preparations of GBS type III PS were evaluated for their specificity in enzyme-linked immunosorbent assay (ELISA): free PS, free PS mixed with methylated human serum albumin (mHSA), PS conjugated to biotin and PS conjugated to human serum albumin. Three groups of human sera were used to evaluate these PS preparations: sera from recipients of a GBS PS vaccine, sera from women receiving a GBS type III PS-tetanus toxoid conjugate vaccine, and sera from nonimmunized healthy women of childbearing age. Estimated antibody concentrations were different depending on the PS preparation used. Using any of the four preparations, we were able to measure ≤0.05 μg of IgG antibody to the GBS type III PS per ml. The specificity of the assay was determined by competitive inhibition with homologous and heterologous PS. The pneumococcal type 14 PS did not inhibit binding of antibody to the native GBS type III PS in sera from adults receiving the GBS PS vaccine or in sera from nonimmunized adults (except serum G9). The pneumococcal type 14 PS inhibited 50% in sera from recipients of GBS type III conjugate vaccine and in serum G9 when GBS type III PS conjugated to biotin or to HSA was used as antigen in ELISA. These data show that free GBS type III PS or PS mixed with mHSA is a sensitive and specific antigen for ELISA and that conjugation can alter the antigenic specificity of a PS.

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