scholarly journals Isolation and determination of bacterial microbiota of Varroa destructor and isolation of Lysinibacillus sp. from it

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Mehtap Usta
Keyword(s):  
Varroa Destructor ◽  
Optimum Growth ◽  
Biochemical Tests ◽  
Bacillus Mycoides ◽  
Important Species ◽  
Bacterial Microbiota ◽  
Pantoea Dispersa ◽  
Bee Health ◽  
Growth Ph

Abstract Background The importance of bees for environmental health is known. Within the scope of this importance, it is of great importance to protect the health of bees and to prevent colony extinction. In this context, it is very important to develop effective methods in combating microorganisms, parasitoids, mites and organisms that cause disease or harm in bees. Both use different methods in terms of bee health. Result In this study, the possibility of the bacteria isolated from Varroa destructor mite being bioinsecticide was investigated. Accordingly, six bacteria were isolated from the mite. Isolated bacteria were analyzed according to biochemical tests, molecular analysis, optimum growth pH and phylogenetic tree drawn as Pantoea dispersa (GV1), Lysinibacillus macroides (GV3), Bacillus mycoides (GV4), Lysinibacillus fusiformis (GV5), Pseodomonas lutea (GV5), Lysinibacillus varians (GV7). Lysinibacillus sp. The entomopathogenic feature of Lysinibacillus sp. ranked it as the most important species. When the insecticidal properties of bacteria were examined, they were determined as 53, 90, 62, 95, 74 and 83% for GV1, GV3, GV4, GV5, GV6 and GV7, respectively. Conclusion Based on these results and literature review, Lysinibacillus sp. species had a high potential to be used as bioinsecticide against V. destructor mite.

scholarly journals In Vitro Evaluation of Acute Toxicity of Five Citrus spp. Essential Oils towards the Parasitic Mite Varroa destructor

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1182
Author(s):  
Roberto Bava ◽  
Fabio Castagna ◽  
Cristian Piras ◽  
Ernesto Palma ◽  
Giuseppe Cringoli ◽  
...  
Keyword(s):  
Essential Oils ◽  
Honey Bee ◽  
Varroa Destructor ◽  
Negative Impact ◽  
Positive Control ◽  
Citrus Limon ◽  
Citrus Paradisi ◽  
Bee Health ◽  
Citrus Spp

Varroa destructor is the most important ectoparasitic mite of honey bees that has a negative impact on bee health and honey production. The control programs are mainly based on the use of synthetic acaricides that are often administered indiscriminately. All this has led to drug resistance that now represent a great concern for honey bee farming. The research for alternative products/methods for mites’ control is now mandatory. The aim of this study was to test whether Citrus spp. essential oils could diminish the growth of the V. destructor mite. In Calabria (southern Italy), plants of the Citrus genus are very common and grow both spontaneously and cultured. The essential oils used in this study were extracted from bergamot (Citrus bergamia), grapefruit (Citrus paradisi), lemon (Citrus limon), orange (Citrus sinensis), and mandarin (Citrus reticulata) by hydrodistillation. Every EO was in vitro tested against V. destructor. Each experimental replicate was performed using 35 viable adult female mites (5 for each EO) collected the same day from the same apiary and included negative controls (5 individuals exposed to acetone only) and positive controls (5 individuals exposed to Amitraz diluted in acetone). The essential oils (Eos) were diluted (0.5 mg/mL, 1 mg/mL, and 2 mg/mL) in HPLC grade acetone to obtain the working solution to be tested (50 µL/tube). Mite mortality was manually assessed after 1 h exposure under controlled conditions. The essential oils that showed the best effectiveness at 0.5 mg/mL were bergamot, which neutralized (dead + inactivated) 80% (p ≤ 0.001) of the parasites; grapefruit, which neutralized 70% (p ≤ 0.001); and lemon, which neutralized 69% of them. Interestingly, the positive control (Amitraz) at the same concentration neutralized 60% of the parasites. These results demonstrate that Calabrian bergamot, grapefruit, and lemon Eos consistently reduced V. destructor viability and open the possibility for their utilization to control this parasite in honey bee farming.

scholarly journals Preparation of Cerium Oxide-MWCNTs Nanocomposite Bulk Modified Carbon Ceramic Electrode: A Sensitive Sensor for Tamoxifen Determination in Human Serum Samples

2021 ◽  
Author(s):  
Sepideh Shafaei ◽  
Elyas Hosseinzadeh ◽  
Gulsah Saydan Kanberoglu ◽  
Balal Khalilzadeh ◽  
Rahim Mohammad-Rezaei
Keyword(s):  
Cerium Oxide ◽  
Limit Of Detection ◽  
Low Cost ◽  
High Electron ◽  
Range Limit ◽  
Serum Samples ◽  
Important Species ◽  
Carbon Ceramic Electrode ◽  
Carbon Ceramic

Abstract In this study, cerium oxide and multi-walled carbon nanotubes nanocomposite was incorporated into the carbon ceramic electrode (CeO2-MWCNTs/CCE) as a renewable electrode for the electrocatalytic purposes. To demonstrate capability of the fabricated electrode, determination of Tamoxifen as an important anticancer drug with differential pulse voltammetry technique was evaluated. Linear range, limit of detection and sensitivity of the developed sensor were found to be 0.2-40 nM, 0.132 nM and 1.478 µA nM-1 cm-2, respectively. Ease of production, low cost and high electron transfer rate of CeO2-MWCNTs/CCE promise it as a novel electro-analytical tool for determination of important species in real samples.

scholarly journals Effects of Bacterial Cell-Free Supernatant on Nutritional Parameters of Apis Mellifera and Their Toxicity Against Varroa Destructor

2020 ◽  
Vol 64 (1) ◽  
pp. 55-66
Author(s):  
Fiorella G. De Piano ◽  
Matias D. Maggi ◽  
Facundo R. Meroi Arceitto ◽  
Marcela C. Audisio ◽  
Martín Eguaras ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Varroa Destructor ◽  
High Concentration ◽  
Colony Losses ◽  
Nutritional Parameters ◽  
Low Concentrations ◽  
Bee Health ◽  
Contact Exposure ◽  
Fat Bodies ◽  
The Relationship

AbstractApis mellifera L. is an essential pollinator that is currently being affected by several stressors that disturb their ecological function and produce colony losses. Colonies are being seriously affected by the ectoparasitic mite Varroa destructor. The relationship between stressors and bee symbionts is being studied in order to enhance bee health. The goal of this study was to evaluate the effect of cell-free supernatants (CFSs) produced by Lactobacillus johnsonii AJ5, Enterococcus faecium SM21 and Bacillus subtilis subsp. subtilis Mori2 on A. mellifera nutritional parameters and their toxicity against V. destructor. Toxicity and survival bioassays were conducted on adult bees with different concentrations of CFSs. Nutritional parameters such as soluble proteins and fat bodies in abdomens were measured. Varroa destructor toxicity was analyzed by a contact exposure method and via bee hemolymph. At low concentrations, two of CFSs tends to enhance bee survival. Remarkably fat bodies maintained their levels with all CFS concentrations in the abdomens, and soluble protein increased at a high concentration of two CFSs. Toxicity against V. destructor was observed only via hemolymph, and results were in agreement with the treatment that produced an increase in bee proteins. Finally, CFS produced by L. johnsonii AJ5 could be a promising natural alternative for strengthening bee health.

scholarly journals ISOLATION OF MODERATELY HALOPHILIC LIPASE PRODUCING BACTERIA FROM SPONGES IN PAHANG COASTAL WATER, MALAYSIA

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Tengku Haziyamin Tengku Abdul Hamid ◽  
Azzmer Azzar Abdul Hamid ◽  
Nurul Husna Padzil
Keyword(s):  
16S Rrna ◽  
Coastal Water ◽  
Agar Plate ◽  
Enterobacter Aerogenes ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Important Species ◽  
Moderately Halophilic ◽  
Lactose Fermentation ◽  
Paenibacillus Illinoisensis

Sponges (Porifera) harbour diverse microorganisms which can be the potential source for microbial enzymes such as lipase. In this study, moderately halophilic lipase producing bacteria were isolated from sponges tissues collected near Balok, at Pahang coastal water. Out of 70 isolates that grew on tributyrin agar plate, only 7 isolates had produced clear zones surrounding their colonies. Out of these, 5 isolates appeared to be gram-positive rod; meanwhile, the other 2 isolates were gram-negative rod in morphologies. These isolates were subjected to several biochemical tests i.e., oxidase, gelatin hydrolysis, lactose fermentation, citrate and motility test, and 16S rRNA gene amplification and sequencing. The results from 16S rRNA sequencing showed that 2 isolates (NHTH 6B and NHTH 28A) were highly similar (>97%) with Paenibacillus illinoisensis; isolate NHTH 26A with Stenotrophomonas pavanii; and isolate NHTH 29A with Enterobacter aerogenes. Phylogenetic analysis on selected isolates (NHTH 6B, NHTH 26A, NHTH 28A and NHTH 29A) with other species from the database showed high bootstrap values of above 50%. This showed that diverse phyla of lipase producing bacteria were isolated from the sponge collected from Pahang coastal water. In the isolation of industrial important species, the presence of pathogenic group of microorganism in this sponge could indicate issues on water quality and safety in this area.

Efficacy of serum laminin measurement for diagnosis of fibrotic liver diseases.

1988 ◽  
Vol 34 (10) ◽  
pp. 2026-2030 ◽  
Author(s):  
J Kropf ◽  
A M Gressner ◽  
A Negwer
Keyword(s):  
Liver Diseases ◽  
Reference Population ◽  
Diagnostic Methods ◽  
Biochemical Tests ◽  
Diagnostic Efficacy ◽  
Fibrotic Liver ◽  
Predictive Values ◽  
Amino Terminal ◽  
Better Than

Abstract We examined the efficacy of laminin assay in serum for diagnosis of fibrotic liver diseases. Values for subjects with liver disease significantly (P less than 0.05) exceeded those for healthy subjects and patients with nonhepatic diseases. At a cutoff value of 1.45 kilo-units(arb.)/L (approximately 330 micrograms/L) and an assumed prevalence of fibrotic liver diseases of 0.5, positive and negative predictive values of the test were 0.97 and 0.83, respectively, for the comparison with a healthy reference population and 0.81 and 0.80 for nonhepatic diseased patients. Increases in laminin concentration were positively correlated with the extent of fibrotic transition of the liver. Discrimination between fibrotic and cirrhotic stages of chronic liver diseases by means of laminin assay was better than with the amino-terminal propeptide of type III procollagen. According to the criteria of diagnostic efficacy, we conclude that determination of laminin in serum improves the possibilities of clinical-chemical diagnosis of liver fibrosis and cirrhosis. However, as commonly true for other biochemical tests, determination of laminin cannot replace conventional diagnostic methods.

Characterization of rhizobia from root nodule and rhizosphere of Vicia faba in Algeria

2018 ◽  
Author(s):  
Benselama Amela ◽  
Tellah Sihem ◽  
Ourem Faiza ◽  
Ounane Sidi Mohamed
Keyword(s):  
Nitrogen Fixation ◽  
Vicia Faba ◽  
Congo Red ◽  
Total Carbon ◽  
Leguminous Plant ◽  
Available Phosphorus ◽  
Good Growth ◽  
Biochemical Tests ◽  
Poor Growth

Nitrogen fixation resulting from mutual symbiosis of rhizobia and cultivated legume plants is therefore critical to food security as it directly affects agricultural production. Biological Nitrogen Fixation (BNF) can be an important factor in sustainable agriculture. A collection of 20 isolates from fresh Nodules of the legume plant Vicia faba was isolated from five soil of Algeria. The soil from each region has undergone physical-chemical analysis : (granulometry, total carbon, organic matter, pH, and electrical conductivity, determination of available phosphorus and determination of total nitrogen). A media viz., Glucose-Peptone Agar (GPA), Congo red, Yeast Mannitol Agar (YMA) containing 2% NaCl were employed to make presumptive decisions on the recognition and classification of the isolated bacterial strains. All the isolates were found with poor absorption of dye Congo red and little or no growth on the media of GPA and without altering the pH. Almost all of the isolates exhibit good growth on 2% NaCl, poor growth on GPA, thus confirming the rhizobia. After biochemical tests like catalase test and citrate utilization test isolates were confirmed. The presence of rhizobia on root nodules of leguminous plant.

Determination of power-time curves of bacterial growth and study of optimum growth temperature and acidity

1995 ◽  
Vol 45 (1-2) ◽  
pp. 93-98 ◽  
Author(s):  
Zhaodong Nan ◽  
Yongjun Liu ◽  
Haitao Sun ◽  
Honglin Zhang ◽  
Shan Qingzhu ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Growth Temperature ◽  
Optimum Growth ◽  
Optimum Growth Temperature

scholarly journals Colorimetric Determination of Amino Acids using Fluorescent Copper Nanoparticle

2019 ◽  
Vol 9 (2S2) ◽  
pp. 748-751
Keyword(s):  
Amino Acids ◽  
Copper Nanoparticles ◽  
Rhodamine B ◽  
Binding Potential ◽  
Colorimetric Determination ◽  
Copper Nanoparticle ◽  
Important Species ◽  
Relative Binding ◽  
The Status

Among the 20 amino acids, cysteine plays a major role in communication of the cells, especially towards immune system and thus developing sensor for cysteine is very important to understand the status of the human health. Copper nanoparticles decorated with Rhodamine B (RBCN) have the potential to detect the biologically important species such as amino acids, especially cysteine. RBCN has been previously has demonstrated for the sensing of host molecules such as cucurbituril based on the relative binding potential of rhodamine B on the surface of copper nanoparticles. Based on that concept, now we have developed the sensor for amino acids, especially for the cysteine

scholarly journals Identification of Pathogenic Bacteria from Diseased Thai Pangas Pangasius hypophthalmus with Their Sensitivity to Antibiotics

2020 ◽  
pp. 7-21
Author(s):  
Moslima Parven ◽  
M. M. Mahbub Alam ◽  
Sarker Md. Ibrahim Khalil ◽  
Aishi Hamom ◽  
Osman Goni ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Antibiotic Sensitivity ◽  
Pseudomonas Sp ◽  
Biochemical Tests ◽  
Important Species ◽  
High Productivity ◽  
Fresh Fish ◽  
Sugar Fermentation ◽  
Antibiotic Sensitivity Test ◽  
Diseased Fish

Thai Pangas (Pangasiaus hypophthalmus) has been emerging as an economically very important species due their faster growth, year round production and high productivity in South-East Asian aquaculture. It has good market value as a food fish due to its good taste and deliciousness with high protein, minerals and fat content. It is also popular as a game fish. Aims: The present study was conducted to identify bacterial pathogens in diseased P. hypophthalmus and evaluate their sensitivity to antibiotics. Methodology: To identify the causativeagent of diseased fish pure culture of bacteria using slant and streak plate techniques and different biochemical tests such as Gram’s Staining, Motility Test, Sugar Fermentation Test, MR-VP test, Indole Test etc. were performed. To assess the sensitivity of the isolated bacteria to antibiotics five antibiotics disks i.e. Ciprofloxacin (5μg), Azithromycin (15μg), Ampicillin/Sulbactam (20μg), Tetracycline (30μg) and Erythromycin (15μg) were used. Results: Three pathogenic bacteria i.e. Aeromonas hydrophila, Edwardsiella ictaluri and Pseudomonas sp. were identified in the studied diseased fish. Only Pseudomonas sp. were identified from Fresh fish. E. ictaluri was found only in diseased pangas which was the causative agent for thedisease, Bacillary Necrosis. The results of the antibiotic sensitivity test showed multi-resistances of the identified bacteria to the tested antibiotics. The identified bacteria were 100% sensitive to Ciprofloxacin (5μg), intermediate to Azithromycin (15μg) and Tetracycline (30μg), but resistant to Erythromycin (15μg) and Ampicilin/Sulbactam (20μg). Conclusion: Ciprofloxacin (5µg) could be used to control bacillary necrosis disease in Thai pangas. The results of this study will be helpful to the fish farmers for the management of bacterial diseases in fish.

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