scholarly journals Breeding Rice lines for physio-functional food through indica ‘Zhaxima’ × japonica ‘Nanjing 46’ haploid technique

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Q. Q. Tian ◽  
X. Li ◽  
C. M. Lu ◽  
X. W. Fang
Keyword(s):  
Oryza Sativa ◽  
Rice Variety ◽  
Short Chain Fatty Acids ◽  
Basic Medium ◽  
Eating Quality ◽  
Induction Medium ◽  
Efficient Technology ◽  
Plant Materials ◽  
Regenerated Plants ◽  
Basic Induction

AbstractResistant starch (RS) encompasses those forms of starch which are not accessible to human digestive enzymes and are fermented in the colons producing short chain fatty acids. The plant materials containing RS are few in the world. In this contribution, the culture ability of callus from anthers of F1 plants from, landraces, ‘Zhaxima’(Oryza sativa var. indica, high-RS rice line with 7.705 ± 0.142, g/100 g) × ‘Nanjing 46’ (Oryza sativa var. japonica, rice variety with RS content (g/100 g) of 0.200 ± 0.001 crosses were studied for obtaining high RS rice plants. The results showed that when M8 basic induction medium was added with 1.5 mg /L 2,4-D、2 mg /LNAA and 0.3 mg /L KT, the inductivity of callus was high as 32.14% for 21 d after pretreatment at 4 °C for 3 d; When MS differentiation basic medium was added with 2 mg /LKT and 3 mg /L ABA, the frequency of regeneration for callus was 50.3% with only a regeneration frequency of 4.55% grown into green seedlings. The RS content in the seeds was between those of the two parents and was partially normally distributed, the highest RS contents of the regenerated plants was as high as 7.66 ± 1.197%. This produced an efficient technology for regenerating stable rice lines with high RS and good eating quality using anthers culture.

scholarly journals Optimizing Osteogenic Differentiation of Ovine Adipose-Derived Stem Cells by Osteogenic Induction Medium and FGFb, BMP2, or NELL1 In Vitro

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Emil Østergaard Nielsen ◽  
Li Chen ◽  
Jonas Overgaard Hansen ◽  
Matilda Degn ◽  
Søren Overgaard ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Basic Medium ◽  
Induction Medium ◽  
Control Analysis ◽  
Alizarin Red ◽  
Osteogenic Induction Medium ◽  
Osteogenic Induction ◽  
Fibroblast Growth Factor Basic

Although adipose-derived stromal cells (ADSCs) have been a major focus as an alternative to autologous bone graft in orthopedic surgery, bone formation potential of ADSCs is not well known and cytokines as osteogenic inducers on ADSCs are being investigated. This study aimed at isolating ADSCs from ovine adipose tissue (AT) and optimizing osteogenic differentiation of ovine ADSCs (oADSC) by culture medium and growth factors. Four AT samples were harvested from two female ovine (Texel/Gotland breed), and oADSCs were isolated and analyzed by flow cytometry for surface markers CD29, CD44, CD31, and CD45. Osteogenic differentiation was made in vitro by seeding oADSCs in osteogenic induction medium (OIM) containing fibroblast growth factor basic (FGFb), bone morphogenetic protein 2 (BMP2), or NEL-like molecule 1 (NELL1) in 4 different dosages (1, 10, 50, and 100 ng/ml, respectively). Basic medium (DMEM) was used as control. Analysis was made after 14 days by Alizarin red staining (ARS) and quantification. This study successfully harvested AT from ovine and verified isolated cells for minimal criteria for adipose stromal cells which suggests a feasible method for isolation of oADSCs. OIM showed significantly higher ARS to basic medium, and FGFb 10 ng/ml revealed significantly higher ARS to OIM alone after 14 days.

scholarly journals The Effect of Concentration of Tobacco (Nicotiana tabacum) Extract on Growth Parameters of Rice (Oryza sativa) Inpari-32

2021 ◽  
Vol 940 (1) ◽  
pp. 012026
Author(s):  
D Sumardi ◽  
M Bahariawan ◽  
R R Maulani ◽  
S Suhandono ◽  
C Novia ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Nicotiana Tabacum ◽  
Growth Parameters ◽  
Rice Variety ◽  
Dry Weight ◽  
Growth And Yield ◽  
Food Crop ◽  
Number Of Leaves ◽  
Generative Phase ◽  
Tobacco Extract

Abstract Rice plants (Oryza sativa) are a significant food crop. Tobacco plants (Nicotiana tabacum) can be beneficial and affect growth due to allelochemical content. The srudy aims to determine the effect of tobacco extract (N. tabacum) on the growth and yield of rice (O. sativa) Inpari-32 variety. The study method used the RBD as an experimental design. Three treatments with different concentrations of tobacco extract and one control with three replications. Growth variables were observed: growth rate, plant height, number of tillers, number of leaves, shoot and root dry weight, flowering age, and phytohormones. Observations of the harvest stage include the weight of stover, number of panicle seeds, the total number of tillers, weight of 1000 seeds, the ratio of the weight of filled grain, and productivity (tons/ha). The results showed that although not significant, tobacco extract treatment increased the number of tillers and leaves, the content of phytohormon IAA in the vegetative phase, the content of GA3 in the generative phase, shoot-root ratio, number of tillers, and accelerated flowering age. The implication of tobacco extract positively affects the plant growth variable and accelerated flowering age of rice variety (O. sativa) Inpari 32.

scholarly journals In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 712
Author(s):  
Marzena Nowakowska ◽  
Žaklina Pavlović ◽  
Marcin Nowicki ◽  
Sarah L. Boggess ◽  
Robert N. Trigiano
Keyword(s):  
Endangered Species ◽  
Shoot Proliferation ◽  
Genetic Fidelity ◽  
Induction Medium ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Regenerated Plants ◽  
Micropropagated Plants ◽  
Ssrs Markers

Helianthus verticillatus (Asteraceae), whorled sunflower, is a perennial species restricted to a few locations in the Southeastern United States. Habitat loss has caused H. verticillatus to become rare, and since 2014, it has been federally listed as an endangered species. As a part of the recovery plan for the restoration and protection of H. verticillatus, an efficient micropropagation protocol based on axillary shoot proliferation was developed. Various concentrations of 6-benzylaminopurine (BAP; 0 to 4.44 µM) were examined for their morphogenetic potential in the regeneration of six genotypes of H. verticillatus from the nodal explants derived from greenhouse-grown plants. Both the BAP concentration and genotype had significant effects on the regeneration capacity of H. verticillatus. Although the induced buds were observed on ½-strength Murashige and Skoog medium without plant growth regulators, a higher rate of induction and bud development were achieved on media with either 0.88 or 2.22 µM BAP, regardless of the genotype. Successful rooting of the induced shoots was achieved within four weeks after the transfer from the induction medium to the fresh ½-strength MS medium, but the rooting efficiency was dependent on the plant’s genetic background. Regenerated plantlets, with well-developed shoots and roots, were acclimatized successfully to greenhouse conditions with a 97% survival rate. Simple sequence repeats (SSRs) markers were employed to assess the genetic uniformity of the micropropagated plants of H. verticillatus. No extraneous bands were detected between regenerants and their respective donor plants, confirming the genetic fidelity and stability of regenerated plants. To our knowledge, the protocol developed in this study is the first such report for this endangered species.

scholarly journals Lectin Sequence Distribution in QTLs from Rice (Oryza sativa) Suggest A Role in Morphological Traits and Stress Responses

2019 ◽  
Vol 20 (2) ◽  
pp. 437
Author(s):  
Mariya Tsaneva ◽  
Kristof De Schutter ◽  
Bruno Verstraeten ◽  
Els J.M. Van Damme
Keyword(s):  
Oryza Sativa ◽  
Stress Responses ◽  
Biotic Resistance ◽  
Eating Quality ◽  
Defense Proteins ◽  
Genetic Method ◽  
Biotic Stresses ◽  
Yield Information ◽  
As Stress

Rice (Oryza sativa) is one of the main staple crops worldwide but suffers from important yield losses due to different abiotic and biotic stresses. Analysis of quantitative trait loci (QTL) is a classical genetic method which enables the creation of more resistant cultivars but does not yield information on the genes directly involved or responsible for the desired traits. Lectins are known as proteins with diverse functions in plants. Some of them are abundant proteins in seeds and are considered as storage/defense proteins while other lectins are known as stress-inducible proteins, implicated in stress perception and signal transduction as part of plant innate immunity. We investigated the distribution of lectin sequences in different QTL related to stress tolerance/resistance, morphology, and physiology through mapping of the lectin sequences and QTL regions on the chromosomes and subsequent statistical analysis. Furthermore, the domain structure and evolutionary relationships of the lectins in O. sativa spp. indica and japonica were investigated. Our results revealed that lectin sequences are statistically overrepresented in QTLs for (a)biotic resistance/tolerance as well as in QTLs related to economically important traits such as eating quality and sterility. These findings contribute to the characterization of the QTL sequences and can provide valuable information to the breeders.

scholarly journals Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang)

2017 ◽  
Vol 18 (2) ◽  
pp. 91
Author(s):  
Kunti Anis Azizah ◽  
Didik Pudji Restanto ◽  
Bambang Sugiharto
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Rice Variety ◽  
Rice Cultivar ◽  
Medium Composition ◽  
Regeneration Frequency ◽  
Optimum Result

Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization. Keywords: Somatic embryo, Indica rice (Oryza sativa L. var. Ciherang), Callus

scholarly journals Evaluation of seedling growth of rice (Oryza sativa L.) genotypes under water stress and non-stress conditions

2020 ◽  
Vol 5 (2) ◽  
pp. 174-178
Author(s):  
Shreejana K. C. ◽  
Kabita Kumari Shah ◽  
Niru Baidhya ◽  
Prashamsa Neupane ◽  
Surakshya Pokhrel ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Water Stress ◽  
Drought Tolerance ◽  
Stress Condition ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Research Work ◽  
Higher Plant ◽  
Water Stress Condition ◽  
Number Of Leaves

Rice (Oryza sativa L.) is a species of crops which is highly sensitive to drought. The sensitivity to water stress varies from genotypes to genotypes of rice. An experiment was performed to compare twenty-five different rice genotypes for drought tolerance in the seedling stage at Gokuleshwor Agriculture and Animal Science College, Baitadi Nepal from July to August 2018. The genotypes were tested under two conditions viz., water stress and non-water stress. The research work was carried out in a completely random design (CRD) with three replications. The results exhibited the height of seedling, length & breadth of leaf, number of leaves/plant decreased, and number of leaves shedding increased under water stress condition. Clustering was done by the method of average linkage, and genotypes were categorized into five clusters. The genotype, namely Radha 11 grouped in Cluster-V had higher plant height, bigger length & breadth of leaf, maximum number of leaves/plant and minimum number of leaf shedding. Thus, this genotype showed the best performance for morphological traits under water stress condition. Therefore, this genotype Radha 11 can be utilized further for developing rice variety with drought-tolerance.

scholarly journals Genome sequence of the model rice variety KitaakeX

2019 ◽  
Author(s):  
Rashmi Jain ◽  
Jerry Jenkins ◽  
Shengqiang Shu ◽  
Mawsheng Chern ◽  
Joel A. Martin ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Rice Plant ◽  
De Novo ◽  
Rice Variety ◽  
High Quality ◽  
Protein Coding ◽  
Genomic Variations ◽  
Protein Coding Genes ◽  
Gene Annotations ◽  
High Quality Genome

AbstractHere, we report the de novo genome sequencing and analysis of Oryza sativa ssp. japonica variety KitaakeX, a Kitaake plant carrying the rice XA21 immune receptor. Our KitaakeX sequence assembly contains 377.6 Mb, consisting of 33 scaffolds (476 contigs) with a contig N50 of 1.4 Mb. Complementing the assembly are detailed gene annotations of 35,594 protein coding genes. We identified 331,335 genomic variations between KitaakeX and Nipponbare (ssp. japonica), and 2,785,991 variations between KitaakeX and Zhenshan97 (ssp. indica). We also compared Kitaake resequencing reads to the KitaakeX assembly and identified 219 small variations. The high-quality genome of the model rice plant KitaakeX will accelerate rice functional genomics.

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