Mitigation of free radical toxicity in hyperoxaluric condition by a novel derivative eicosapentaenoate-lipoate

2002 ◽  
Vol 21 (3) ◽  
pp. 153-158 ◽  
Author(s):  
M Lenin ◽  
LM Latha ◽  
M Nagaraj ◽  
P Varalakshmi
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Antioxidant Status ◽  
Stone Formation ◽  
Ammonium Oxalate ◽  
Supporting Evidence ◽  
Antioxidant Defence ◽  
Stone Formers ◽  
Implantation Method ◽  
Foreign Body Implantation

Lipoic acid (LA) and eicosapentaenoic acid (EPA) have been shown to ameliorate the changes associated with hyperoxaluria. This prompted us to study the effect of EPA-LA, a new derivative, in experimental urolithiatic condition. Foreign body implantation method followed by supple-mentation of ammonium oxalate was adopted to induce stone formation in the bladder. Significant depletion in the antioxidant status was observed in the kidney and bladder of stone-forming animals, associated with increased lipid peroxidation. The present observations provide supporting evidence to the hypothesis that free radicals might be involved in causing toxicity in hyperoxaluric condition. The three drugs, namely LA, EPA and EPA-LA had reversed the above changes, but the effect was more pronounced in EPA-LA-treated stone formers. These features highlight the beneficial effect of EPA-LA wherein the potency of two drugs has been combined. The practical outcome of these findings is that the cellular antioxidant defence can be increased by the supplementation of lipoate and its derivative EPA-LA. Human & Experimental Toxicology (2002) 21, 153–158.

scholarly journals Rate of lipid peroxidation in brain and liver tissues and the total antioxidant status of blood plasma in developing chicks

2001 ◽  
Vol 49 (2) ◽  
pp. 197-202
Author(s):  
N. Balogh ◽  
T. Gaál ◽  
F. Husvéth ◽  
P. Vajdovich
Keyword(s):  
Lipid Peroxidation ◽  
Blood Plasma ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Broiler Chicks ◽  
Antioxidant Defence ◽  
Plasma Antioxidant Capacity ◽  
Total Antioxidant ◽  
Plasma Antioxidant ◽  
The Brain

Age-related changes of tissue lipid peroxidation (LPO) of liver and brain, as well as plasma antioxidant capacity of broiler chicken cockerels were investigated. Tissue LPO was characterised by the spectrophotometric assessment of thiobarbituric acid reactive substances (TBARS). Plasma antioxidant power was evaluated by the measurement of total antioxidant status (TAS). Newly hatched broiler chicks had similar TAS value (1.19 mmol/l) as newborns of mammalian species. Significant changes (p < 0.05) were observed in the time course of all parameters. Tissue TBARS concentration was higher in the brain than in the liver at hatching, while the latter organ was found to have more effective antioxidant defence during embryonic life. The concentration of TBARS increased up to the 10th day in the liver but only up to the 21st day in the brain, and the former was accompanied by an approximately 50% decrease of plasma antioxidant capacity. This suggests that the liver plays an important role in forming the antioxidant defence mechanisms of the blood plasma in broiler chicks.

scholarly journals UV-vis spectroscopic study of lipid peroxidation and antioxidant contents of kidney of Albino mice co-administrated with sildenafil citrate and alcohol

2011 ◽  
Vol 26 (4-5) ◽  
pp. 279-288
Author(s):  
K. Soumya ◽  
Raju Udayakumar ◽  
K. Viswanathan
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Free Radicals ◽  
Spectroscopic Study ◽  
Sildenafil Citrate ◽  
Antioxidant Defence ◽  
Defence System ◽  
Antioxidant Defence System ◽  
Albino Mice ◽  
Enzymic Antioxidants

Male Wistar Albino mice were co-administrated with appropriate dose of sildenafil citrate and alcohol. The kidney samples of those animals were analyzed for lipid peroxidation and antioxidant contents. Lipid peroxidation was measured based on TBARS. Antioxidant such as SOD, CAT, GPx and GSH were also determined. From the present investigation, an enhanced level of TBARS accompanied by a decreased concentration of enzymic as well as non-enzymic antioxidants in kidney was detected for 30 days animals treated concomitantly with sildenafil citrate and alcohol. Increased level of lipid peroxidation lead to accumulation of free radicals and increased oxidative threat in kidney tissues. Also, the decreased concentration of antioxidant enzymes in renal tissues indicates the failure of antioxidant defence system.

Phenolic constituents and inhibitory effects of the leaf of Rauvolfia vomitoria Afzel on free radicals, cholinergic and monoaminergic enzymes in rat’s brain in vitro

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Olubukola H. Oyeniran ◽  
Adedayo O. Ademiluyi ◽  
Ganiyu Oboh
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Methanolic Extract ◽  
Array Detector ◽  
Oh Radicals ◽  
Inhibitory Effects ◽  
Methanolic Extracts ◽  
Phenolic Constituents ◽  
Rauvolfia Vomitoria

AbstractObjectivesRauvolfia vomitoria is a medicinal plant used traditionally in Africa in the management of several human diseases including psychosis. However, there is inadequate scientific information on the potency of the phenolic constituents of R. vomitoria leaf in the management of neurodegeneration. Therefore, this study characterized the phenolic constituents and investigated the effects of aqueous and methanolic extracts of R. vomitoria leaf on free radicals, Fe2+-induced lipid peroxidation, and critical enzymes linked to neurodegeneration in rat’s brain in vitro.MethodsThe polyphenols were evaluated by characterizing phenolic constituents using high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). The antioxidant properties were assessed through the extracts ability to reduce Fe3+ to Fe2+; inhibit ABTS, DPPH, and OH radicals and Fe2+-induced lipid peroxidation. The effects of the extracts on AChE and MAO were also evaluated.ResultsThe phenolic characterization of R. vomitoria leaf revealed that there were more flavonoids present. Both aqueous and methanolic extracts of R. vomitoria leaf had inhibitory effects with the methanolic extract having higher significant (p≤0.05) free radicals scavenging ability coupled with inhibition of monoamine oxidases. However, there was no significant (p≤0.05) difference obtained in the inhibition of lipid peroxidation and cholinesterases.ConclusionThis study suggests that the rich phenolic constituents of R. vomitoria leaf might contribute to the observed antioxidative and neuroprotective effects. The methanolic extract was more potent than the aqueous extract; therefore, extraction of R. vomitoria leaf with methanol could offer better health-promoting effects in neurodegenerative condition.

Ethane production as a measure of lipid peroxidation after renal ischemia

1986 ◽  
Vol 251 (5) ◽  
pp. F839-F843 ◽  
Author(s):  
M. S. Paller ◽  
R. P. Hebbel
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Superoxide Radical ◽  
Oxygen Free Radicals ◽  
Tissue Injury ◽  
Renal Ischemia ◽  
Radical Scavenger ◽  
Base Line ◽  
Ethane Production

After renal ischemia, oxygen free radicals are formed and produce tissue injury, in large part, through peroxidation of polyunsaturated fatty acids. We used an in vivo method to monitor lipid peroxidation after renal ischemia, the measurement of ethane in expired gas, to determine the time course of lipid peroxidation and the effect of several agents to limit lipid peroxidation after renal ischemia. In anesthetized rats there was no significant increase in ethane production during 60 min of renal ischemia. During the first 10 min of renal reperfusion, there was a prompt increase in ethane production from 2.9 +/- 1.3 to 6.3 +/- 1.9 pmol/min (P less than 0.05). Ethane production was significantly increased during the first 50 min of reperfusion and then rapidly tapered to base-line levels. Preischemic administration of allopurinol to prevent superoxide radical generation or the superoxide radical scavenger superoxide dismutase prevented the increase in ethane production during postischemic reperfusion. These studies confirm that there is increase lipid peroxidation following renal ischemia that can be prevented by agents which limit the formation or accumulation of oxygen free radicals. This in vivo method for measuring lipid peroxidation could also be employed to study the effects of ischemia on lipid peroxidation in other organs, as well as to monitor lipid peroxidation in other forms of injury.

scholarly journals Malondialdehyde levels can be measured in serum and saliva by using a fast HPLC method with visible detection / Determinarea printr-o metodă HPLC-VIS rapidă a concentraţiilor serice şi salivare ale malondialdehidei

2016 ◽  
Vol 24 (3) ◽  
pp. 319-326 ◽  
Author(s):  
Erzsébet Fogarasi ◽  
Mircea Dumitru Croitoru ◽  
Ibolya Fülöp ◽  
Enikő Nemes-Nagy ◽  
Robert Gabriel Tripon ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Free Radicals ◽  
Unsaturated Fatty Acids ◽  
Pearson Correlation ◽  
Living Organism ◽  
Hplc Method ◽  
Limit Of Quantification ◽  
Pearson Correlation Test ◽  
Repeated Sample

Abstract Oxidative stress appears when the amount of free radicals that are formed in a living organism exceed its spin-trapping ability. One of the most dangerous free radicals that are formed in the human body is the hydroxyl radical. It can alter several biomolecules, including the unsaturated fatty acids; this process is known as lipid peroxidation and can lead to cell necrosis and generation of several harmful byproducts including malondialdehyde, which serves also as a biomarker of oxidative stress. A new HPLC method with visible detection was developed for the detection of malondialdehyde in human serum and saliva samples. The method was verified in terms of specificity, linearity, limits of detection (0.35 ng/ml), limit of quantification (1.19 ng/ml), recovery (90.13±10.25 – 107.29±14.33) and precision (3.84±1.49% – 6.66±1.76%). An analysis time of only 1 minute was obtained and no interferences from the matrices were observed. Statistical analysis (Pearson correlation test) showed a moderate correlation (R = 0.5061, p = 0.0099) between serum and saliva concentrations (N = 25). The possibility of measuring salivary concentrations of malondialdehyde extents the applications of oxidative stress/lipid peroxidation estimations to categories of population unreachable before (pregnant women, small children, etc); repeated sample studies are also easier to make.

Blood antioxidant status and erythrocyte lipid peroxidation following distance running

1990 ◽  
Vol 282 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Garry G. Duthie ◽  
John D. Robertson ◽  
Ronald J. Maughan ◽  
Philip C. Morrice
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Distance Running ◽  
Erythrocyte Lipid

Manganese neurotoxicity: a model for free radical mediated neurodegeneration?

1982 ◽  
Vol 60 (11) ◽  
pp. 1398-1405 ◽  
Author(s):  
John Donaldson ◽  
Duncan McGregor ◽  
Frank LaBella
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Neuropsychiatric Symptoms ◽  
Brain Regions ◽  
Neonatal Rats ◽  
Manganese Neurotoxicity ◽  
Neuronal Homeostasis ◽  
Significant Depression ◽  
Disease Study ◽  
Bizarre Behavior

In man, manganese neurointoxication is characterised in the early phase by bizarre behavior reminiscent of that observed in schizophrenia. During chronic manganese intoxication the neuropsychiatric symptoms manifested earlier disappear and are followed by a permanent neurological phase typified by extrapyramidal symptoms similar to those of Parkinson's disease. Study of manganese intoxication in animals may provide important clues towards elucidation of the biochemical defect underlying neuropsychiatric as well as extrapyramidal diseases. Investigations in our laboratory suggest that neurotoxicity of manganese is an exaggeration of function in normal neuronal homeostasis. Manganese neurointoxication in neonatal rats resulted in significant depression of lipid peroxidation in several rat brain regions examined. In the striatum, lipid peroxidative activity was abolished, an effect which may be related to alteration in neurotransmitters often observed in the striatum of manganese-treated rats. The chronic, extrapyramidal stage of manganism, may ensue when excess Mn2+ is oxidised to higher valency forms where it can potentiate the autoxidation of catecholamines, like dopamine, resulting in concomitant formation of free radicals and cytotoxic quinones. This latter effect may arise preferentially in the substantia nigra, where neuromelanin is formed nonenzymatically by autoxidation of dopamine.

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