Zebrafish Model of Lipid Metabolism for Drug Discovery

2004 ◽  
pp. 423-431
Author(s):  
Steven Farber ◽  
Amy Rubinstein ◽  
Shiu-Ying Ho
Keyword(s):  
Lipid Metabolism ◽  
Drug Discovery ◽  
Zebrafish Model

scholarly journals Zebrafish Model in Ophthalmology to Study Disease Mechanism and Drug Discovery

2021 ◽  
Vol 14 (8) ◽  
pp. 716
Author(s):  
Yiwen Hong ◽  
Yan Luo
Keyword(s):  
Drug Discovery ◽  
Behavioral Assessment ◽  
Age Related Macular Degeneration ◽  
Disease Mechanism ◽  
Zebrafish Model ◽  
Age Related ◽  
Eye Disorders ◽  
Vertebrate Model ◽  
Drug Treatments ◽  
Novel Drug

Visual impairment and blindness are common and seriously affect people’s work and quality of life in the world. Therefore, the effective therapies for eye diseases are of high priority. Zebrafish (Danio rerio) is an alternative vertebrate model as a useful tool for the mechanism elucidation and drug discovery of various eye disorders, such as cataracts, glaucoma, diabetic retinopathy, age-related macular degeneration, photoreceptor degeneration, etc. The genetic and embryonic accessibility of zebrafish in combination with a behavioral assessment of visual function has made it a very popular model in ophthalmology. Zebrafish has also been widely used in ocular drug discovery, such as the screening of new anti-angiogenic compounds or neuroprotective drugs, and the oculotoxicity test. In this review, we summarized the applications of zebrafish as the models of eye disorders to study disease mechanism and investigate novel drug treatments.

Plasmalogens improve swimming performance by modulating the expression of genes involved in amino acids and lipid metabolism, oxidative stress, and ferroptosis in an Alzheimer's disease zebrafish model

2021 ◽  
Author(s):  
Junli Feng ◽  
Gongshuai Song ◽  
Yuanyuan Wu ◽  
Xi Chen ◽  
Jie Pang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Lipid Metabolism ◽  
Human Health ◽  
Swimming Performance ◽  
Cognitive Impairments ◽  
Zebrafish Model ◽  
Expression Of Genes ◽  
Health Studies ◽  
Underlying Mechanisms

Plasmalogens (PLs) are critical to human health. Studies have reported a link between downregulation of PLs levels and cognitive impairments in patients with Alzheimer´s disease (AD). however, the underlying mechanisms...

scholarly journals Analysis of mycobacterial infection-induced changes to host lipid metabolism in a zebrafish infection model reveals a conserved role for LDLR in infection susceptibility

2018 ◽  
Author(s):  
Matt D. Johansen ◽  
Elinor Hortle ◽  
Joshua A. Kasparian ◽  
Alejandro Romero ◽  
Beatriz Novoa ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Low Density Lipoprotein ◽  
Lipoprotein Metabolism ◽  
Density Lipoprotein ◽  
Mycobacterial Infection ◽  
Infection Model ◽  
Zebrafish Model ◽  
Marine Research ◽  
Functional Relevance ◽  
Induced Changes

AbstractChanges to lipid metabolism are well-characterised consequences of human tuberculosis infection but their functional relevance are not clearly elucidated in these or other host-mycobacterial systems. The zebrafish-Mycobacterium marinum infection model is used extensively to model many aspects of human-M. tuberculosis pathogenesis but has not been widely used to study the role of infection-induced lipid metabolism. We find mammalian mycobacterial infection-induced alterations in host Low Density Lipoprotein metabolism are conserved in the zebrafish model of mycobacterial pathogenesis. Depletion of LDLR, a key lipid metabolism node, decreased M. marinum burden, and corrected infection-induced altered lipid metabolism resulting in decreased LDL and reduced the rate of macrophage transformation into foam cells. Our results demonstrate a conserved role for infection-induced alterations to host lipid metabolism, and specifically the LDL-LDLR axis, across host-mycobacterial species pairings.FundingThis work was supported by the Australian National Health and Medical Research Council (APP1099912 and APP1053407 to S.H.O.); Meat and Livestock Australia (P.PSH. 0813 to A.C.P. and K. dS); the Marie Bashir Institute for Infectious Diseases and Biosecurity (grant to S.H.O., A.C.P. and K. dS); the Kenyon Family Foundation Inflammation Award (grant to S.H.O.); the University of Sydney (fellowship to S.H.O.); Consellería de Economía, Emprego e Industria (GAIN), Xunta de Galicia (grant IN607B 2016/12 to Institute of Marine Research (IIM-CSIC)).

scholarly journals Zebrafish as a Model System to Study the Mechanism of Cutaneous Wound Healing and Drug Discovery: Advantages and Challenges

2021 ◽  
Vol 14 (10) ◽  
pp. 1058
Author(s):  
Ruth Naomi ◽  
Hasnah Bahari ◽  
Muhammad Dain Yazid ◽  
Hashim Embong ◽  
Fezah Othman
Keyword(s):  
Wound Healing ◽  
Drug Discovery ◽  
Drug Formulation ◽  
New Drugs ◽  
Current Evidence ◽  
Cutaneous Wound Healing ◽  
Zebrafish Model ◽  
Signaling Mechanism ◽  
Cutaneous Wound

In humans, cutaneous wounds may heal without scars during embryogenesis. However, in the adult phase, the similar wound may undergo a few events such as homeostasis, blood clotting, inflammation, vascularization, and the formation of granulation tissue, which may leave a scar at the injury site. In consideration of this, research evolves daily to improve the healing mechanism in which the wound may heal without scarring. In regard to this, zebrafish (Danio rerio) serves as an ideal model to study the underlying signaling mechanism of wound healing. This is an important factor in determining a relevant drug formulation for wound healing. This review scrutinizes the biology of zebrafish and how this favors the cutaneous wound healing relevant to the in vivo evidence. This review aimed to provide the current insights on drug discovery for cutaneous wound healing based on the zebrafish model. The advantages and challenges in utilizing the zebrafish model for cutaneous wound healing are discussed in this review. This review is expected to provide an idea to formulate an appropriate drug for cutaneous wound healing relevant to the underlying signaling mechanism. Therefore, this narrative review recapitulates current evidence from in vivo studies on the cutaneous wound healing mechanism, which favours the discovery of new drugs. This article concludes with the need for zebrafish as an investigation model for biomedical research in the future to ensure that drug repositions are well suited for human skin.

scholarly journals Zebrafish-Based Screening Models for the Identification of Anti-Metastatic Drugs

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2407
Author(s):  
Joji Nakayama ◽  
Hideki Makinoshima
Keyword(s):  
Animal Model ◽  
Drug Discovery ◽  
Cancer Cells ◽  
High Throughput Screening ◽  
Metastatic Tumor ◽  
Tumor Formation ◽  
Zebrafish Model ◽  
Step Process ◽  
Physiological Relevance

Metastasis, a leading contributor to the morbidity of cancer patients, occurs through a multi-step process: invasion, intravasation, extravasation, colonization, and metastatic tumor formation. Each process is not only promoted by cancer cells themselves but is also affected by their microenvironment. Given this complexity, drug discovery for anti-metastatic drugs must consider the interaction between cancer cells and their microenvironments. The zebrafish is a suitable vertebrate animal model for in vivo high-throughput screening studies with physiological relevance to humans. This review covers the zebrafish model used to identify anti-metastatic drugs.

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