scholarly journals Conditional Mutagenesis of Gata6 in SF1-Positive Cells Causes Gonadal-Like Differentiation in the Adrenal Cortex of Mice

Endocrinology ◽  
2013 ◽  
Vol 154 (5) ◽  
pp. 1754-1767 ◽  
Author(s):  
Marjut Pihlajoki ◽  
Elisabeth Gretzinger ◽  
Rebecca Cochran ◽  
Antti Kyrönlahti ◽  
Anja Schrade ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Adrenal Cortex ◽  
Adrenal Glands ◽  
Conditional Knockout ◽  
Hormonal Changes ◽  
Corticosterone Secretion ◽  
Steroidogenic Cell ◽  
Nulliparous Female ◽  
And Function

Abstract Transcription factor GATA6 is expressed in the fetal and adult adrenal cortex and has been implicated in steroidogenesis. To characterize the role of transcription factor GATA6 in adrenocortical development and function, we generated mice in which Gata6 was conditionally deleted using Cre-LoxP recombination with Sf1-cre. The adrenal glands of adult Gata6 conditional knockout (cKO) mice were small and had a thin cortex. Cytomegalic changes were evident in fetal and adult cKO adrenal glands, and chromaffin cells were ectopically located at the periphery of the glands. Corticosterone secretion in response to exogenous ACTH was blunted in cKO mice. Spindle-shaped cells expressing Gata4, a marker of gonadal stroma, accumulated in the adrenal subcapsule of Gata6 cKO mice. RNA analysis demonstrated the concomitant upregulation of other gonadal-like markers, including Amhr2, in the cKO adrenal glands, suggesting that GATA6 inhibits the spontaneous differentiation of adrenocortical stem/progenitor cells into gonadal-like cells. Lhcgr and Cyp17 were overexpressed in the adrenal glands of gonadectomized cKO vs control mice, implying that GATA6 also limits sex steroidogenic cell differentiation in response to the hormonal changes that accompany gonadectomy. Nulliparous female and orchiectomized male Gata6 cKO mice lacked an adrenal X-zone. Microarray hybridization identified Pik3c2g as a novel X-zone marker that is downregulated in the adrenal glands of these mice. Our findings offer genetic proof that GATA6 regulates the differentiation of steroidogenic progenitors into adrenocortical cells.

Role of Transcription Factor Oct4 in Postnatal Development and Function of the Adrenal Cortex

2019 ◽  
Vol 167 (4) ◽  
pp. 568-573
Author(s):  
N. V. Yaglova ◽  
S. S. Obernikhin ◽  
S. V. Nazimova ◽  
V. V. Yaglov
Keyword(s):  
Transcription Factor ◽  
Postnatal Development ◽  
Adrenal Cortex ◽  
And Function

scholarly journals The role of Hath6, a newly identified shear-stress-responsive transcription factor, in endothelial cell differentiation and function

2014 ◽  
Vol 127 (7) ◽  
pp. 1428-1440 ◽  
Author(s):  
F. Fang ◽  
S. M. Wasserman ◽  
J. Torres-Vazquez ◽  
B. Weinstein ◽  
F. Cao ◽  
...  
Keyword(s):  
Shear Stress ◽  
Transcription Factor ◽  
Endothelial Cell ◽  
Cell Differentiation ◽  
Endothelial Cell Differentiation ◽  
And Function

scholarly journals Role of the Trps1 transcription factor in odontoblasts differentiation and function

2009 ◽  
Vol 331 (2) ◽  
pp. 436-437
Author(s):  
Dobrawa Napierala ◽  
Izabela Maciejewska ◽  
Elda Munivez ◽  
Brian Dawson ◽  
Rena D'Souza ◽  
...  
Keyword(s):  
Transcription Factor ◽  
And Function

scholarly journals The R2R3-MYB transcription factor family in Taxus chinensis: identification, characterization, expression profiling and posttranscriptional regulation analysis

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8473
Author(s):  
Xinling Hu ◽  
Lisha Zhang ◽  
Iain Wilson ◽  
Fenjuan Shao ◽  
Deyou Qiu
Keyword(s):  
Transcription Factor ◽  
Posttranscriptional Regulation ◽  
Gene Families ◽  
Myb Transcription Factor ◽  
Taxus Chinensis ◽  
Transcription Factor Family ◽  
Myb Genes ◽  
And Function ◽  
R2r3 Myb

The MYB transcription factor family is one of the largest gene families playing regulatory roles in plant growth and development. The MYB family has been studied in a variety of plant species but has not been reported in Taxus chinensis. Here we identified 72 putative R2R3-MYB genes in T. chinensis using a comprehensive analysis. Sequence features, conversed domains and motifs were characterized. The phylogenetic analysis showed TcMYBs and AtMYBs were clustered into 36 subgroups, of which 24 subgroups included members from T. chinensis and Arabidopsis thaliana, while 12 subgroups were specific to one species. This suggests the conservation and specificity in structure and function of plant R2R3-MYBs. The expression of TcMYBs in various tissues and different ages of xylem were investigated. Additionally, miRNA-mediated posttranscriptional regulation analysis revealed that TcMYBs were the targets of miR858, miR159 and miR828, suggesting the posttranscriptional regulation of MYBs is highly conserved in plants. The results provide a basis for further study the role of TcMYBs in the regulation of secondary metabolites of T. chinensis.

scholarly journals FOXL2: a central transcription factor of the ovary

2013 ◽  
Vol 52 (1) ◽  
pp. R17-R33 ◽  
Author(s):  
Adrien Georges ◽  
Aurelie Auguste ◽  
Laurianne Bessière ◽  
Anne Vanet ◽  
Anne-Laure Todeschini ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Forkhead Transcription Factor ◽  
Gene Encoding ◽  
Post Translational Modifications ◽  
Forkhead Box ◽  
Molecular Aspects ◽  
And Function ◽  
Craniofacial Defects ◽  
The Impact

Forkhead box L2 (FOXL2) is a gene encoding a forkhead transcription factor preferentially expressed in the ovary, the eyelids and the pituitary gland. Its germline mutations are responsible for the blepharophimosis ptosis epicanthus inversus syndrome, which includes eyelid and mild craniofacial defects associated with primary ovarian insufficiency. Recent studies have shown the involvement of FOXL2 in virtually all stages of ovarian development and function, as well as in granulosa cell (GC)-related pathologies. A central role of FOXL2 is the lifetime maintenance of GC identity through the repression of testis-specific genes. Recently, a highly recurrent somatic FOXL2 mutation leading to the p.C134W subtitution has been linked to the development of GC tumours in the adult, which account for up to 5% of ovarian malignancies. In this review, we summarise data on FOXL2 modulators, targets, partners and post-translational modifications. Despite the progresses made thus far, a better understanding of the impact of FOXL2 mutations and of the molecular aspects of its function is required to rationalise its implication in various pathophysiological processes.

scholarly journals The Regulatory Role of Activating Transcription Factor 2 in Inflammation

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Tao Yu ◽  
Yong Jun Li ◽  
Ai Hong Bian ◽  
Hui Bin Zuo ◽  
Ti Wen Zhu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Leucine Zipper ◽  
Regulatory Role ◽  
Neurological Development ◽  
Potential Applications ◽  
Inflammatory Drugs ◽  
Activating Transcription Factor ◽  
And Function ◽  
Potential Inhibitors

Activating transcription factor 2 (ATF2) is a member of the leucine zipper family of DNA-binding proteins and is widely distributed in tissues including the liver, lung, spleen, and kidney. Like c-Jun and c-Fos, ATF2 responds to stress-related stimuli and may thereby influence cell proliferation, inflammation, apoptosis, oncogenesis, neurological development and function, and skeletal remodeling. Recent studies clarify the regulatory role of ATF2 in inflammation and describe potential inhibitors of this protein. In this paper, we summarize the properties and functions of ATF2 and explore potential applications of ATF2 inhibitors as tools for research and for the development of immunosuppressive and anti-inflammatory drugs.

scholarly journals Dpy30 is critical for maintaining the identity and function of adult hematopoietic stem cells

2016 ◽  
Vol 213 (11) ◽  
pp. 2349-2364 ◽  
Author(s):  
Zhenhua Yang ◽  
Kushani Shah ◽  
Alireza Khodadadi-Jamayran ◽  
Hao Jiang
Keyword(s):  
Significant Loss ◽  
Conditional Knockout ◽  
H3k4 Methylation ◽  
Hematopoietic Stem ◽  
Conditional Knockout Mouse ◽  
Stem And Progenitor Cells ◽  
Severe Pancytopenia ◽  
And Function ◽  
Methylation Activity

As the major histone H3K4 methyltransferases in mammals, the Set1/Mll complexes play important roles in animal development and are associated with many diseases, including hematological malignancies. However, the role of the H3K4 methylation activity of these complexes in fate determination of hematopoietic stem and progenitor cells (HSCs and HPCs) remains elusive. Here, we address this question by generating a conditional knockout mouse for Dpy30, which is a common core subunit of all Set1/Mll complexes and facilitates genome-wide H3K4 methylation in cells. Dpy30 loss in the adult hematopoietic system results in severe pancytopenia but striking accumulation of HSCs and early HPCs that are defective in multilineage reconstitution, suggesting a differentiation block. In mixed bone marrow chimeras, Dpy30-deficient HSCs cannot differentiate or efficiently up-regulate lineage-regulatory genes, and eventually fail to sustain for long term with significant loss of HSC signature gene expression. Our molecular analyses reveal that Dpy30 directly and preferentially controls H3K4 methylation and expression of many hematopoietic development-associated genes including several key transcriptional and chromatin regulators involved in HSC function. Collectively, our results establish a critical and selective role of Dpy30 and the H3K4 methylation activity of the Set1/Mll complexes for maintaining the identity and function of adult HSCs.

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