scholarly journals Regulation of skeletal muscle fiber type and slow myosin heavy chain 2 gene expression by inositol trisphosphate receptor 1

2005 ◽  
Vol 118 (10) ◽  
pp. 2295-2302 ◽  
Author(s):  
T. Jordan
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Muscle Fiber ◽  
Heavy Chain ◽  
Fiber Type ◽  
Skeletal Muscle Fiber ◽  
Inositol Trisphosphate Receptor ◽  
Slow Myosin Heavy Chain ◽  
Trisphosphate Receptor

scholarly journals Calcineurin plays a modulatory role in loading-induced regulation of type I myosin heavy chain gene expression in slow skeletal muscle

2009 ◽  
Vol 297 (4) ◽  
pp. R1037-R1048 ◽  
Author(s):  
Clay E. Pandorf ◽  
Weihua H. Jiang ◽  
Anqi X. Qin ◽  
Paul W. Bodell ◽  
Kenneth M. Baldwin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Fiber Type ◽  
Hindlimb Suspension ◽  
Chain Gene ◽  
Type I ◽  
Thyroid State

The role of calcineurin (Cn) in skeletal muscle fiber-type expression has been a subject of great interest because of reports indicating that it controls the slow muscle phenotype. To delineate the role of Cn in phenotype remodeling, particularly its role in driving expression of the type I myosin heavy chain (MHC) gene, we used a novel strategy whereby a profound transition from fast to slow fiber type is induced and examined in the absence and presence of cyclosporin A (CsA), a Cn inhibitor. To induce the fast-to-slow transition, we first subjected rats to 7 days of hindlimb suspension (HS) + thyroid hormone [triiodothyronine (T3)] to suppress nearly all expression of type I MHC mRNA in the soleus muscle. HS + T3 was then withdrawn, and rats resumed normal ambulation and thyroid state, during which vehicle or CsA (30 mg·kg−1·day−1) was administered for 7 or 14 days. The findings demonstrate that, despite significant inhibition of Cn, pre-mRNA, mRNA, and protein abundance of type I MHC increased markedly during reloading relative to HS + T3 ( P < 0.05). Type I MHC expression was, however, attenuated by CsA compared with vehicle treatment. In addition, type IIa and IIx MHC pre-mRNA, mRNA, and relative protein levels were increased in Cn-treated compared with vehicle-treated rats. These findings indicate that Cn has a modulatory role in MHC transcription, rather than a role as a primary regulator of slow MHC gene expression.

scholarly journals A Calcineurin-NFATc3-Dependent Pathway Regulates Skeletal Muscle Differentiation and Slow Myosin Heavy-Chain Expression

2000 ◽  
Vol 20 (17) ◽  
pp. 6600-6611 ◽  
Author(s):  
Ulrike Delling ◽  
Jolana Tureckova ◽  
Hae W. Lim ◽  
Leon J. De Windt ◽  
Peter Rotwein ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Signal Transduction ◽  
Gene Transfer ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myogenic Differentiation ◽  
Fiber Type ◽  
Slow Myosin ◽  
Slow Myosin Heavy Chain ◽  
Myocyte Differentiation

ABSTRACT The differentiation and maturation of skeletal muscle cells into functional fibers is coordinated largely by inductive signals which act through discrete intracellular signal transduction pathways. Recently, the calcium-activated phosphatase calcineurin (PP2B) and the family of transcription factors known as NFAT have been implicated in the regulation of myocyte hypertrophy and fiber type specificity. Here we present an analysis of the intracellular mechanisms which underlie myocyte differentiation and fiber type specificity due to an insulinlike growth factor 1 (IGF-1)–calcineurin–NFAT signal transduction pathway. We demonstrate that calcineurin enzymatic activity is transiently increased during the initiation of myogenic differentiation in cultured C2C12 cells and that this increase is associated with NFATc3 nuclear translocation. Adenovirus-mediated gene transfer of an activated calcineurin protein (AdCnA) potentiates C2C12 and Sol8 myocyte differentiation, while adenovirus-mediated gene transfer of noncompetitive calcineurin-inhibitory peptides (cain or ΔAKAP79) attenuates differentiation. AdCnA infection was also sufficient to rescue myocyte differentiation in an IGF-depleted myoblast cell line. Using 10T1/2 cells, we demonstrate that MyoD-directed myogenesis is dramatically enhanced by either calcineurin or NFATc3 cotransfection, while a calcineurin inhibitory peptide (cain) blocks differentiation. Enhanced myogenic differentiation directed by calcineurin, but not NFATc3, preferentially specifies slow myosin heavy-chain expression, while enhanced differentiation through mitogen-activated protein kinase kinase 6 (MKK6) promotes fast myosin heavy-chain expression. These data indicate that a signaling pathway involving IGF-calcineurin-NFATc3 enhances myogenic differentiation whereas calcineurin acts through other factors to promote the slow fiber type program.

Growth rate in beef cattle affects adipose gene expression and skeletal muscle fiber type

2013 ◽  
pp. 389-390
Author(s):  
P. A. Lancaster ◽  
M. A. Vaughn ◽  
J. D. Starkey ◽  
E. D. Sharman ◽  
C. R. Krehbiel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Growth Rate ◽  
Beef Cattle ◽  
Muscle Fiber ◽  
Fiber Type ◽  
Skeletal Muscle Fiber ◽  
Muscle Fiber Type

scholarly journals Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition

Physiology ◽  
2015 ◽  
Vol 30 (1) ◽  
pp. 30-39 ◽  
Author(s):  
K. M. Haizlip ◽  
B. C. Harrison ◽  
L. A. Leinwand
Keyword(s):  
Skeletal Muscle ◽  
Thyroid Hormone ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Contractile Function ◽  
Fiber Type ◽  
Skeletal Muscle Fiber ◽  
Male And Female ◽  
Type Composition ◽  
Males And Females

Previous studies have identified over 3,000 genes that are differentially expressed in male and female skeletal muscle. Here, we review the sex-based differences in skeletal muscle fiber composition, myosin heavy chain expression, contractile function, and the regulation of these physiological differences by thyroid hormone, estrogen, and testosterone. The findings presented lay the basis for the continued work needed to fully understand the skeletal muscle differences between males and females.

scholarly journals Genome-wide expression analysis and EMX2 gene expression in embryonic myoblasts committed to diverse skeletal muscle fiber type fates

2013 ◽  
Vol 242 (8) ◽  
pp. 1001-1020 ◽  
Author(s):  
Kristina Weimer ◽  
Jillian Theobald ◽  
Kenneth S. Campbell ◽  
Karyn A. Esser ◽  
Joseph X. DiMario
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Expression Analysis ◽  
Muscle Fiber ◽  
Fiber Type ◽  
Skeletal Muscle Fiber ◽  
Muscle Fiber Type ◽  
Genome Wide ◽  
Genome Wide Expression
Sign in / Sign up

Export Citation Format

Share Document