scholarly journals A guide to accurate reporting in digital image processing – can anyone reproduce your quantitative analysis?

2021 ◽  
Vol 134 (6) ◽  
pp. jcs254151 ◽  
Author(s):  
Jesse Aaron ◽  
Teng-Leong Chew

ABSTRACTConsiderable attention has been recently paid to improving replicability and reproducibility in life science research. This has resulted in commendable efforts to standardize a variety of reagents, assays, cell lines and other resources. However, given that microscopy is a dominant tool for biologists, comparatively little discussion has been offered regarding how the proper reporting and documentation of microscopy relevant details should be handled. Image processing is a critical step of almost any microscopy-based experiment; however, improper, or incomplete reporting of its use in the literature is pervasive. The chosen details of an image processing workflow can dramatically determine the outcome of subsequent analyses, and indeed, the overall conclusions of a study. This Review aims to illustrate how proper reporting of image processing methodology improves scientific reproducibility and strengthens the biological conclusions derived from the results.

2012 ◽  
Vol 531 ◽  
pp. 547-553 ◽  
Author(s):  
Li Xin Zhang ◽  
Shuai Ling Wei ◽  
Zheng Guang Xu ◽  
Xue Chong Ren ◽  
Li Li Lin ◽  
...  

In the quantitative analysis of metallographic organization, the grain size is a fundamental parameter. The traditional methods of getting the grain size depend on artificiality. These means have strong subjectivity and low efficiency. In this paper, a new digital image processing technology method is proposed for the measurement of the grain size. The experiment with the 7050 Al-alloy samples show that the proposed algorithm has performed well compared with the artificial methods.


An important measure of proof collection, storage, and authentication in forensic sciences, which decide the safety and security of any system documents, which can be either portable document formats or scanned images. To gather evidence, or plan a forensic investigation digital images are secured with different modern methodologies. Digital image analysis includes image recovery and surveillance for image information improvement. The goal of forgery detection is to maximize the extraction of information from manipulated images, particularly noisy and post-processed images. Because digital image processing is becoming popular with many advantages in scientific and engineering applications, the forgery techniques are also growing at a rapid rate. Therefore, the main focus is on different types of forgery detection in digital image processing with the help of all transform techniques and comparing their best results for further improvement in order to generate a new approach for a future forensic science investigation.


Author(s):  
R. C. Gonzalez

Interest in digital image processing techniques dates back to the early 1920's, when digitized pictures of world news events were first transmitted by submarine cable between New York and London. Applications of digital image processing concepts, however, did not become widespread until the middle 1960's, when third-generation digital computers began to offer the speed and storage capabilities required for practical implementation of image processing algorithms. Since then, this area has experienced vigorous growth, having been a subject of interdisciplinary research in fields ranging from engineering and computer science to biology, chemistry, and medicine.


Author(s):  
L. Montoto ◽  
M. Montoto ◽  
A. Bel-Lan

INTRODUCTION.- The physical properties of rock masses are greatly influenced by their internal discontinuities, like pores and fissures. So, these need to be measured as a basis for interpretation. To avoid the basic difficulties of measurement under optical microscopy and analogic image systems, the authors use S.E.M. and multiband digital image processing. In S.E.M., analog signal processing has been used to further image enhancement (1), but automatic information extraction can be achieved by simple digital processing of S.E.M. images (2). The use of multiband image would overcome difficulties such as artifacts introduced by the relative positions of sample and detector or the typicals encountered in optical microscopy.DIGITAL IMAGE PROCESSING.- The studied rock specimens were in the form of flat deformation-free surfaces observed under a Phillips SEM model 500. The SEM detector output signal was recorded in picture form in b&w negatives and digitized using a Perkin Elmer 1010 MP flat microdensitometer.


Author(s):  
J. Hefter

Semiconductor-metal composites, formed by the eutectic solidification of silicon and a metal silicide have been under investigation for some time for a number of electronic device applications. This composite system is comprised of a silicon matrix containing extended metal-silicide rod-shaped structures aligned in parallel throughout the material. The average diameter of such a rod in a typical system is about 1 μm. Thus, characterization of the rod morphology by electron microscope methods is necessitated.The types of morphometric information that may be obtained from such microscopic studies coupled with image processing are (i) the area fraction of rods in the matrix, (ii) the average rod diameter, (iii) an average circularity (roundness), and (iv) the number density (Nd;rods/cm2). To acquire electron images of these materials, a digital image processing system (Tracor Northern 5500/5600) attached to a JEOL JXA-840 analytical SEM has been used.


Author(s):  
K. N. Colonna ◽  
G. Oliphant

Harmonious use of Z-contrast imaging and digital image processing as an analytical imaging tool was developed and demonstrated in studying the elemental constitution of human and maturing rabbit spermatozoa. Due to its analog origin (Fig. 1), the Z-contrast image offers information unique to the science of biological imaging. Despite the information and distinct advantages it offers, the potential of Z-contrast imaging is extremely limited without the application of techniques of digital image processing. For the first time in biological imaging, this study demonstrates the tremendous potential involved in the complementary use of Z-contrast imaging and digital image processing.Imaging in the Z-contrast mode is powerful for three distinct reasons, the first of which involves tissue preparation. It affords biologists the opportunity to visualize biological tissue without the use of heavy metal fixatives and stains. For years biologists have used heavy metal components to compensate for the limited electron scattering properties of biological tissue.


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