The Changes in the Tensile Properties of Tendons after Freeze Storage in Saline Solution

Author(s):  
Boon Ho Ng ◽  
Siaw Meng Chou ◽  
Beng Hai Lim ◽  
Alphonsus Chong

The objective of this study is to evaluate the influence of saline solution (0.9 per cent NaCl) on the tensile properties of freeze-stored tendons. Firstly, 170 pieces of chicken flexor digitorum profundus tendons were retrieved and wrapped in saline-soaked gauze before they were stored at -40 °. Then specimens were tensile tested at various time points over 360 days, scanning electron microscopy (SEM) was performed on fresh specimens, and specimens were freeze-stored for 233 days to investigate microstructure change after freeze storage. The mean values of strain ultimate tensile strength (UTS) did not deviate significantly (analysis of variance; p = 0.249) following freeze storage while the UTS and elastic modulus increased gradually with the duration of freeze storage and the growth became significant ( p < 0.01) for durations longer than 70 and 40 days respectively. The SEM study showed that the collagen fibre density of specimens stored for 233 days decreased because of porosity growth. These findings suggested that the saline increased the tensile strength and modulus of the collagen.

2003 ◽  
Vol 03 (03n04) ◽  
pp. 299-308 ◽  
Author(s):  
BOON-HO NG ◽  
SIAW-MENG CHOU

The objective of the current study is to elucidate the influence of freeze storage on the tensile properties of tendons by testing specimens at numerous adjacent durations to eliminate the influence of biological variation and experimental errors. Chicken flexor digitorum profundus tendons were stored intact in the digits at -20°C and specimens were tensile tested at 33 durations over 360 days. Scanning electron microscopy was done on fresh specimens and specimens freeze-stored for 30, 233 and 427 days to investigate microstructure change after freeze storage. The tensile properties of tendons, as represented by ultimate tensile strength, strained at ultimate tensile strength. The elastic modulus did not deviate significantly (p>0.01) after freeze storage while the collagen fiber density of specimens stored for 233 and 427 days decreased with porosity growth. In contrast, no obvious microstructure difference was observed between fresh tendons and tendons stored for 30 days. These findings suggest that the postmortem degradation incurred a minor effect on the structural integrity of the tendons and therefore did not affect the overall tensile properties. We conclude that freeze storage at -20°C does not alter the tensile properties of tendons for as many as 360 days.


2003 ◽  
Vol 28 (5) ◽  
pp. 478-482 ◽  
Author(s):  
J. TAN ◽  
B. WANG ◽  
B. TAN ◽  
Y. XU ◽  
J. B. TANG

We performed a study to evaluate the tensile properties of partial tendon lacerations and the effects of peripheral sutures on the tendon strength. Seventy-two fresh pig flexor digitorum profundus tendons were divided equally into eight groups. Tendons in four of the groups were subjected to partial lacerations (60%, 70%, 80%, and 90%) and were not repaired. In the other four similar groups partial lacerations were repaired with running peripheral sutures. The tendons were subjected to load-to-failure tests in an Instron tensile machine to determine the initial, 1 mm, 2 mm gap formation forces and the ultimate strength. The tendons with lacerations of 80% and 90% had a remarkably lower tensile strength than those 60% and 70% lacerations. Running peripheral sutures increased the gap formation forces and the ultimate strength of all the tendons, though particularly those with 80% and 90% lacerations.


Hand Surgery ◽  
2013 ◽  
Vol 18 (03) ◽  
pp. 375-379 ◽  
Author(s):  
Muntasir Mannan Choudhury ◽  
Shian Chao Tay

Surgical treatment for trigger finger involves division of the A1 pulley. Some surgeons perform an additional step of traction tenolysis by sequentially bringing the flexor digitorum superficialis and flexor digitorum profundus tendons out of the wound gently with a Ragnell retractor. There is currently no study which states whether flexor tendon traction tenolysis should be routinely performed or not. The objective of this study is to compare the outcome in patients who have traction tenolysis performed (A group) versus those who did not have traction tenolysis (B group) performed. It was noted that even though the mean total active motion (TAM) for the B group in our study was lower preoperatively, it was consistently higher than the A group in all the 3 post-operative visits demonstrating a better outcome in the B group. Even though it was not statistically significant, our data also showed that patients with traction tenolysis appeared to have more postoperative pain compared to those without.


1998 ◽  
Vol 23 (1) ◽  
pp. 37-40 ◽  
Author(s):  
L. GORDON ◽  
M. TOLAR ◽  
K. T. VENKATESWARA RAO ◽  
R. O. RITCHIE ◽  
S. RABINOWITZ ◽  
...  

We have developed a stainless steel internal tendon anchor that is used to strengthen a tendon repair. This study tested its use in vitro to produce a repair that can withstand the tensile strength demands of early active flexion. Fresh human cadaver flexor digitorum profundus tendons were harvested, divided, and then repaired using four different techniques: Kessler, Becker or Savage stitches, or the internal tendon anchor. The internal splint repairs demonstrated a 99–270% increase in mean maximal linear tensile strength and a 49–240% increase in mean ultimate tensile strength over the other repairs. It is hoped that this newly developed internal anchor will provide a repair that will be strong enough to allow immediate active range of motion.


2012 ◽  
Vol 37 (2) ◽  
pp. 101-108 ◽  
Author(s):  
T. H. Low ◽  
T. S. Ahmad ◽  
E. S. Ng

We have compared a simple four-strand flexor tendon repair, the single cross-stitch locked repair using a double-stranded suture (dsSCL) against two other four-strand repairs: the Pennington modified Kessler with double-stranded suture (dsPMK); and the cruciate cross-stitch locked repair with single-stranded suture (Modified Sandow). Thirty fresh frozen cadaveric flexor digitorum profundus tendons were transected and repaired with one of the core repair techniques using identical suture material and reinforced with identical peripheral sutures. Bulking at the repair site and tendon–suture junctions was measured. The tendons were subjected to linear load-to-failure testing. Results showed no significant difference in ultimate tensile strength between the Modified Sandow (36.8 N) and dsSCL (32.6 N) whereas the dsPMK was significantly weaker (26.8 N). There were no significant differences in 2 mm gap force, stiffness or bulk between the three repairs. We concluded that the simpler dsSCL repair is comparable to the modified Sandow repair in tensile strength, stiffness and bulking.


2006 ◽  
Vol 21 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Elcio Shiyoiti Hirano ◽  
Mario Mantovani ◽  
Rosana Celestina Morandin ◽  
Jarbas de Brito ◽  
Lilian Pavani

PURPOSE: To evaluate and compare neutrophils sequestration in the renal cortex of rats, result of total hepatic ischemia and reperfusion after controlled hemorrhagic shock, with use of different electrolytic solutions. METHODS: Used 18 rats Wistar, males, adult, divided into three groups as the solution used to reanimation: Group PSS: physiologic saline solution; Group HSS: hypertonic saline hypertonic (7,5%) followed by lactated ringer's solution; Group LRS: lactated ringer's solution. All the animals were submitted to the bleeding controlled until mean arterial pressure (MAP) 40 mmHg, for 20 minutes. Performed volemic replacement until PAM=80 mmHg with the solution according the studied group, followed by laparotomy and Pringle's Maneuver for 15 minutes. The animals were accompanied until for two hours. To statistical comparisons between mean of neutrophils sequestration, in interstitium of the renal cortex, were made the tests One-way ANOVA and covariance analysis, adjusting itself for time of supervened. The hemodynamic parameters evaluated were: MAP, heat rate, cardiac index, vascular resistance system index. The analyzed metabolic variables were: pH, bicarbonate, base deficit and lactato, besides electrolytes. RESULTS: The mean values of supervened, in minutes, for group were: Group PSS 79,0±12,0; Group LRS 97,0±11,0; Group HSS 67,0±10. The mean values of neutrophils/field in the renal cortex were: Group PSS 0,55±0,68; Group LRS 1,68±0,53; Group HSS 1,33±0,43. When adjusted for time of supervened: Group PSS 0,55; Group LRS 1,62; Group HSS 1,39. There was statistically significant difference in neutrophils sequestration, between Group PSS regarding the others groups, using itself or not the adjustment by time of supervened (p=0,016 and p=0,0128). CONCLUSION: Both critical situations in this model, controlled hemorrhagic shock followed by Pringle's maneuver, promoted neutrophils sequestration in the interstitium renal of rat, and the physiologic saline solution demonstrated minor mean, differentiating statistically of the others solutions.


2005 ◽  
Vol 284-286 ◽  
pp. 905-908
Author(s):  
Jae Hyup Lee ◽  
Hyun Seung Ryu ◽  
Jun Hyuk Seo ◽  
Hwan Kim ◽  
Kug Sun Hong ◽  
...  

The purpose of our study is to compare the biodegradation and osteoconduction between CaO-SiO2-B2O3 glass-ceramics(CS10B) and hydroxyapatite(HA), tricalcium phosphate(TCP). Porous CS10B implants were prepared by polymer sponge method. Single-level posterolateral spinal fusions were performed on thirty rabbits. The animals were divided into three groups by implant material: HA, TCP and CS10B. Radiographs checked every two weeks. All animals were sacrificed 12 weeks after surgery. The proportion of the area occupied by ceramics in final radiography on the initial radiography was calculated. Uniaxial tensile strength was measured on 7 cases in each group. The proportion of the area of HA(88.7%±16.1) was significantly higher than those of the others(p<0.05), and the proportion of the area of CS10B(28.2%±9.3) was significantly lower than those of HA and TCP(37%±9.6)(p<0.05). The mean values of tensile strength of the HA(191.4±33.5 N) and CS10B(182.7±19.9 N) were significantly higher(p<0.05) than those of the TCP(141.1±28.2 N). CS10B showed the tensile strength of fusion masses similar to those of the HA, however, more rapid biodegradation than HA and TCP. These findings suggest that CS10B grafts have the possibility as a bone graft substitute.


2017 ◽  
Vol 22 (01) ◽  
pp. 46-52
Author(s):  
Mokhtar Abdul Azeem ◽  
Yousef Marwan ◽  
Ahmed El Morshidy ◽  
Ali Esmaeel ◽  
Yehia Zakaria

Background: Closed avulsion injury of the flexor digitorum profundus (FDP) tendon is a relatively common condition of the hand. Its present classification system seems to be deficient in including all possible patterns of injury, leading to improper selection of the best treatment method for the injury. We aim to provide a new classification scheme for this injury. Methods: We developed a new classification scheme based on the possible pattern of FDP tendon injury. It consisted of three main types, and 10 sub-types. This was used to classify the injury of 34 patients, and help in choosing the best management approach. All patients underwent surgical treatment of their injury. The distal interphalangeal (DIP) joint extension deficit and total active motion (TAM) of the proximal interphalangeal (PIP) and DIP joints were considered as the main outcomes. Five orthopaedic surgeons used the new classification system for our cases, and the inter-rater reproducibility was tested with Fleiss' kappa. Results: The multi-rater kappa for the classification was excellent. At the final follow-up visit 24 months following surgery, the mean loss of extension of the DIP joint was 13.76° ± 13.53° (range 0° to 45°), while the mean TAM was 148.88° ± 22.64° (range 94° to 172°). Based on the TAM score, 21 (61.76%), 8 (23.53%) and 5 (14.71%) patients had excellent, good and fair results, respectively. None of our patients had poor results. Conclusions: Our new classification scheme of FDP tendon avulsion appeared to be comprehensive and useful in guiding the surgeon for the best treatment option. Nevertheless, this should be confirmed by using it for larger number of patients with different patterns of injury.


2007 ◽  
Vol 32 (3) ◽  
pp. 302-307 ◽  
Author(s):  
M. SIEMIONOW ◽  
G. AGAOGLU ◽  
R. HOFFMANN

This study describes the characteristics of a fascia overlying the ulnar nerve for 10 cm distal to the midpoint of the retrocondylar groove. A total of 28 cadaver upper extremities were dissected. The ulnar nerve between the flexor carpi ulnaris and flexor digitorum profundus was traced distally underneath a thin fascia. The length of the fascia was measured and examined for the presence of segmental fascial thickenings, referred to as ‘Bands’. Two types of fascia were found. In Type I, three Bands were identified within the fascia and the mean length of the fascia was 5.6 cm. In Type II, four Bands were identified and the mean length of the fascia was 7.7 cm. The presence of Bands within the fascia overlying the ulnar nerve in the proximal forearm may require release at the time of decompression, or anterior transposition, of the ulnar nerve at the cubital tunnel.


2021 ◽  
Vol 880 ◽  
pp. 117-124
Author(s):  
Harini Sosiati ◽  
Angga Ardinista ◽  
M. Dirga Rianto ◽  
Ardhia Revarti ◽  
Sinin Hamdan ◽  
...  

The commercially available Curcuma mangga Val extract (CMVE) and poly (vinyl alcohol) (PVA) blends were studied by electrospinning. The result showed inadequate tensile properties of commercial CMVE/PVA nanofiber mats with very high modulus (220.0 MPa) and low strain (50%). The commercial CMVE was then replaced by a type of non-commercial CMVE to compare them with those of the commercial bandage. A non-commercial CMVE was prepared by simply extracting of the fresh Curcuma mangga Val (CMV) produced in a liquid extract. This non-commercial CMVE was then blended with 10% (w/w) PVA at various concentrations of CMVE (0, 2, 5, 10 and 15%) (w/w). The morphology and tensile properties of the mats were investigated. The findings indicated that the tensile strength and modulus increased with CMVE concentrations, decreased of the mean fiber diameter. In this study, a nanofiber mat with a concentration of 2% CMVE was selected and compared to others. This mat had lower average fiber diameter (187.50 nm), tensile strength (8.93 ± 0.36 MPa) and modulus (31.37 ± 4.91MPa), and higher tensile strain (90%) than the commercial CMVE/PVA. Compared to the tensile properties of the Hansaplast bandage, the current tensile strength was higher, the tensile modulus was equivalent and lower tensile strain.


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