Purpose:The purpose of this study was to use a novel accelerometer metric, the minimum acceleration value above which the most active accumulated x-min are accumulated, and to apply it to data collected in primary school children to explore its utility for reporting the most active 30-min during the school day. The aims were to (i) investigate associations between the most active 30-min during the school day (M30ACC) metric and health indicators, and (ii) demonstrate that applying an equivalent cut-point to the M30ACC metric will give similar, and therefore comparable prevalence results as a traditional moderate-to-vigorous physical activity (MVPA) cut-point approach.Methods:Data were available for 297 children (156 girls; age 10 years) who wore wrist-mounted accelerometers for 7-days. School day MVPA and the minimum acceleration value above which the most active 30-min were accumulated during school (M30ACC) were calculated. Body mass index (BMI), waist-to-height ratio (WHtR), and cardiorespiratory fitness (CRF) (number of 20-m shuttle run test (SRT) laps) were also measured. Mixed linear models investigated associations between M30ACC and health indicators. Agreement between ranked MVPA and M30ACC values was assessed using percent agreement, kappa, sensitivity, and specificity statistics. Results:The mean M30ACC value was 242.7 (99.3) mg, and mean school day MVPA was 34.3 (14.8) min. M30ACC thresholds related to health indicators were 213 mg (BMI), 206 mg (WHtR), and 269 mg (CRF) for girls. The equivalent values for boys were 234mg (BMI), 230 mg (WHtR), and 327 mg (CRF). The 30 min school day MVPA guideline averaged for the week and on every valid day was achieved by 54.9% and 24.9% of the sample, respectively. 63.0% of the participants achieved the equivalent M30ACC threshold averaged for the week, and 20.2% achieved it on every valid day of the week. Less than half of girls and 75% of boys accumulated 30 min of school day MVPA. Just less than 50% of girls and >80% of boys had M30ACC values ≥200 mg, which is equivalent to brisk walking. Agreement between MVPA and M30ACC tertiles was high, reflected by the agreement (89.2% to 94.6%), Kappa scores (0.76 to 0.86), and values for sensitivity and specificity, which exceeded 90%.Conclusions:The results demonstrate the utility of M30ACC as a PA metric that is based on measured accelerations and is not heavily influenced by researcher decisions. M30ACC has potential as an accelerometer-specific metric for generating PA guidelines related to health indicators, and easily understood forms of activity, such as brisk walking.