Assessment of markers of bone formation under controlled environmental factors and their correlation with serum minerals in adult sheep as a model for orthopaedic research

2008 ◽  
Vol 42 (4) ◽  
pp. 465-472 ◽  
Author(s):  
I R Dias ◽  
C A Viegas ◽  
J T De Azevedo ◽  
E M Costa ◽  
P Lourenço ◽  
...  
Keyword(s):  
Normal Values ◽  
Healing Process ◽  
Average Weight ◽  
Serum Electrolyte ◽  
Blood Samples ◽  
Non Invasive ◽  
Serum Minerals ◽  
The Mean ◽  
Bone Healing Process ◽  
Markers Of Bone Formation

Summary Eighteen healthy skeletally mature (3 years old) ewes, with an average weight of 45 kg, of the Portuguese Churra da Terra Quente breed were used to evaluate the normal values of total and bone-specific isoform of alkaline phosphatase serum activities (ALP and BALP, respectively) and serum osteocalcin (OC) and their correlation with the serum minerals - calcium (Ca), phosphorus (P), magnesium (Mg) and ionized calcium (Ca2+). The sheep were maintained under controlled environmental conditions (constant diurnal photoperiod cycle and identical husbandry and feeding) for six weeks before the collection of the blood samples. The measurement of the total ALP and serum minerals was performed with automated biochemistry analysers using the BioMérieux® kits, the serum electrolyte Ca2+ Diametrics Medical, Inc® specific cassettes and the BALP and OC METRATM kits from QUIDEL® Corporation. The mean ± standard deviation values obtained were: total ALP 90.17 ± 85.72 U/L, BALP 15.0 ± 5.44 U/L, ratio BALP/ total ALP 29.28 ± 24.22, OC 13.02 ± 1.87 ng/mL, Ca 2.57 ± 0.37 mmol/L, P 2.13 ± 0.42 mmol/L, Mg 1.04 ± 0.13 mmol/L, Ca2+ 1.29 ± 0.04 mmol/L. Significant correlations were observed between the total ALP and Ca ( r = 0.5939; P = 0.05) and OC and Ca ( r = 0.5706; P = 0.05). Reference to the serum values of bone turnover parameters in sheep could be of great value in research and could provide complementary non-invasive information on the bone healing process, particularly with regard to obtaining an early prognosis of fracture healing.

Abstract 15974: Non-Invasive Myocardial Work Indices in Normal Adolescents: A Pilot Study

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Sarah Pradhan ◽  
Anna Mullikin ◽  
Vien T Truong ◽  
Wojciech Mazur ◽  
Michael D Taylor ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Pilot Study ◽  
Normal Values ◽  
Global Longitudinal Strain ◽  
Lv Function ◽  
Healthy Adolescent ◽  
Non Invasive ◽  
Work Index ◽  
The Mean ◽  
Global Work

Introduction: Myocardial work may provide a different perspective on LV function and energy consumption. Its non-invasive assessment by echocardiography correlates with invasive measures and there are normal values established in adult patients. We aimed to establish normal values in a healthy adolescent population. Methods: Nineteen healthy subjects (mean age = 15 ± 2 years, 42% male) with normal echocardiograms were prospectively included. Brachial cuff blood pressure was obtained immediately following apical images in the supine position. Post-processing of echocardiograms with speckle tracking echocardiography and derivation of global myocardial work indices from LV pressure-strain loops was done using EchoPAC (General Electric, v203R73.0) (Figure 1). Results: Baseline demographics, echocardiographic measures and global myocardial work indices are reported in Table 1. The mean global work index is 1833 ± 278 mmHg with mean global work efficiency of 95 ± 1.3%. No gender difference in myocardial work indices are found (p>0.05 for all). Correlation between global work indices with age, systolic blood pressure, LV ejection fraction, and global longitudinal strain (GLS) are presented in Table 2. Conclusions: This is a pilot study to begin establishing normal adolescent indices of non-invasive myocardial work. Figure 1. Example

Simplified radioimmunoassay of bradykinin in human plasma.

1980 ◽  
Vol 26 (3) ◽  
pp. 429-432
Author(s):  
P S Verma ◽  
P E Lorenz ◽  
G E Sander
Keyword(s):  
Molecular Mass ◽  
Human Plasma ◽  
Normal Values ◽  
Normal Subjects ◽  
Single Step ◽  
Relative Molecular Mass ◽  
Blood Samples ◽  
Analytical Recovery ◽  
Assay Tube ◽  
The Mean

Abstract A greatly simplified radioimmunoassay for bradykinin in human plasma is described. Current techniques require multiple chromatographic steps or extraction procedures with analytical recoveries of bradykinin of often less than 60%. We present a method in which bradykinin is separated from components of higher relative molecular mass (including kininogens) in a single step, by use of a column of Sephadex G-25 medium (PD-10). The mean analytical recovery of tritiated bradykinin added to plasma is 85.5% (SD, 3.5%). The sensitivity of this radioimmunoassay is 25 pg per assay tube, equivalent to 125 ng per liter of plasma. Twenty to 30 blood samples may be completely processed and assayed within 6 h. As determined with this technique, concentrations of bradykinin in plasma from apparently normal subjects ranged from 2.5 to 5.2 microgram/L (mean 4.2, SD 1.1 microgram/L); these values are consistent with previously reported normal values.

Simplified radioimmunoassay of bradykinin in human plasma.

1980 ◽  
Vol 26 (3) ◽  
pp. 429-432 ◽  
Author(s):  
P S Verma ◽  
P E Lorenz ◽  
G E Sander
Keyword(s):  
Molecular Mass ◽  
Human Plasma ◽  
Normal Values ◽  
Normal Subjects ◽  
Single Step ◽  
Relative Molecular Mass ◽  
Blood Samples ◽  
Analytical Recovery ◽  
Assay Tube ◽  
The Mean

Abstract A greatly simplified radioimmunoassay for bradykinin in human plasma is described. Current techniques require multiple chromatographic steps or extraction procedures with analytical recoveries of bradykinin of often less than 60%. We present a method in which bradykinin is separated from components of higher relative molecular mass (including kininogens) in a single step, by use of a column of Sephadex G-25 medium (PD-10). The mean analytical recovery of tritiated bradykinin added to plasma is 85.5% (SD, 3.5%). The sensitivity of this radioimmunoassay is 25 pg per assay tube, equivalent to 125 ng per liter of plasma. Twenty to 30 blood samples may be completely processed and assayed within 6 h. As determined with this technique, concentrations of bradykinin in plasma from apparently normal subjects ranged from 2.5 to 5.2 microgram/L (mean 4.2, SD 1.1 microgram/L); these values are consistent with previously reported normal values.

scholarly journals Conformation of the clinical diagnoses of some malnutrition diseases in local and Shammi goats in Baghdad province

2012 ◽  
Vol 36 (1) ◽  
pp. 8-14
Author(s):  
Al-Shawi A.F. S.
Keyword(s):  
Body Temperature ◽  
Normal Values ◽  
Mean Body Temperature ◽  
Local Breed ◽  
Blood Samples ◽  
Clinical Diagnoses ◽  
The Mean ◽  
Phosphorus And Potassium ◽  
Hb Concentration ◽  
The Relationship

The relationship between the clinical singes and some of the hematological and biochemical values in 126 cases of local breed and Shammi breed goats (from both sexes) diagnosed as cases of malnutrition from 230 goats examined .The clinical examination include (beside the general inspection and case history) body temperature, respiratory and pulse rates, mucus membranes skin and coat. Examination of the blood samples included RBCs count, Hb concentration, PCV % and values of cupper, magnesium, phosphorus, and potassium. The results showed that the mean body temperature of these goats was lower than in normal goats, while the respiratory and pulse rates were higher .Examination of blood revealed decrease in the means of RBCs counts ,Hb concentration and PCV % in the clinically diagnosed cases , and the decrease was more sever in local goats . However the results indicated lower values of cupper, magnesium, phosphorus, and potassium in comparison with normal values in goats. The ratio of cupper deficiency was the highest followed by phosphorus, magnesium and potassium and the ratio of malnutrition was high in Baghdad province. It was in Shammi breed higher than in local breed.

scholarly journals Conformation of the clinical diagnoses of some malnutrition diseases in local and Shammi goats in Baghdad province

2012 ◽  
Vol 36 (1) ◽  
pp. 8-14
Author(s):  
Al-Shawi A.F. S.
Keyword(s):  
Body Temperature ◽  
Normal Values ◽  
Mean Body Temperature ◽  
Local Breed ◽  
Blood Samples ◽  
Clinical Diagnoses ◽  
The Mean ◽  
Phosphorus And Potassium ◽  
Hb Concentration ◽  
The Relationship

The relationship between the clinical singes and some of the hematological and biochemical values in 126 cases of local breed and Shammi breed goats (from both sexes) diagnosed as cases of malnutrition from 230 goats examined .The clinical examination include (beside the general inspection and case history) body temperature, respiratory and pulse rates, mucus membranes skin and coat. Examination of the blood samples included RBCs count, Hb concentration, PCV % and values of cupper, magnesium, phosphorus, and potassium. The results showed that the mean body temperature of these goats was lower than in normal goats, while the respiratory and pulse rates were higher .Examination of blood revealed decrease in the means of RBCs counts ,Hb concentration and PCV % in the clinically diagnosed cases , and the decrease was more sever in local goats . However the results indicated lower values of cupper, magnesium, phosphorus, and potassium in comparison with normal values in goats. The ratio of cupper deficiency was the highest followed by phosphorus, magnesium and potassium and the ratio of malnutrition was high in Baghdad province. It was in Shammi breed higher than in local breed.

Plasma Free Carnitine Levels in 0–12-Month-Old Infants in Relation to Feeding Styles

2006 ◽  
Vol 76 (3) ◽  
pp. 117-123
Author(s):  
Selda Hizel ◽  
Fatma Kaya Kiliç ◽  
Cihat Sanli ◽  
Turgay Coskun ◽  
Saniye Önal
Keyword(s):  
Normal Values ◽  
Free Carnitine ◽  
Tandem Mass ◽  
Carnitine Level ◽  
Blood Samples ◽  
Feeding Styles ◽  
Feeding Style ◽  
Breastfed Infants ◽  
The Mean ◽  
Guthrie Cards

This study was planned to determine the blood carnitine levels of children aged 0–1 year in Kırıkkale. Blood samples were taken on Guthrie cards and plasma free carnitine levels were analyzed by tandem mass spectrometry. The mean free carnitine level was 25.12 ± 10.1 μmol/L (10.1–49.5 μmol/L). To assess the plasma free carnitine levels according to feeding style, babies were grouped as exclusively breastfed (n = 67, 49.3%), artificially fed (n = 24, 17.7%), and mixed fed (n = 45, 33%). The exclusively breastfed infants displayed the lowest free carnitine levels (p < 0.05). However, when this was analyzed in accordance with age, no significant change was observed in plasma free carnitine levels according to feeding style. Results of this study are important as they reveal an indication about the normal values of plasma free carnitine in Turkish infants and their relationship to feeding styles.

scholarly journals Normal ranges of global left ventricular myocardial work indices in adults: a meta-analysis

2021 ◽  
Vol 42 (Supplement_1) ◽  
Author(s):  
T Ngo ◽  
V Truong ◽  
T Phan ◽  
T Pham ◽  
T Nguyen ◽  
...  
Keyword(s):  
Normal Values ◽  
Meta Analysis ◽  
Left Ventricular ◽  
Work Efficiency ◽  
Non Invasive ◽  
Normal Ranges ◽  
Work Index ◽  
Constructive Work ◽  
The Mean ◽  
Global Work

Abstract Background Non-invasive global myocardial work recently emerged as new parameter to characterize left ventricle function with potential advantages over both ejection fraction and global longitudinal strain. Purpose We aimed to perform a meta-analysis of normal ranges of non-invasive left ventricular myocardial work (MW) indices including global constructive work (GCW), global work index (GWI), global wasted work (GWW), and global work efficiency (GWE) and to identify confounding factors that may contribute to variance in reported measures. Methods The authors searched four databases, Pubmed, Scopus, Embase, and Cochrane Library through January 2021 using the key terms “myocardial work”,“global constructive work”, “global wasted work”, “global work index”, “global work efficiency”. Studies were included if the articles reported LV myocardial work using 2D transthoracic echocardiography in healthy normal subjects, either in the control group or comprising the entire study cohort. The weighted mean was estimated by using the random effect model with a 95% confidence interval. Heterogeneity across studies was assessed using the I2 test. Publication bias was examined by funnel plot and Egger's regression test. Results The search yielded 476 articles. After abstract and full text screening we included 13 datasets with 1665 patients for meta-analysis. The reported normal mean values of GCW and GWI among the studies were 2278 (95% CI, 2167 to 23878; I2=95%), and 2.010 (95% CI, 1922 to 2098, I2=97%), respectively. The mean GWE was 96.0 (95% CI, 95.6% to 96.5; I2=92%), and the mean GWW was 79.7% (95% CI, 68.8% to 90.7%; I2=90%) (Figure). Furthermore, age and gender did not significantly contribute to variations in normal values. No evidence of significant publication bias was observed in the funnel plots and the Egger test. Conclusion In this meta-analysis, we provide echocardiographic reference ranges for non-invasive indices of MW. These normal values should serve as a template for clinical and research use for this promising technology. FUNDunding Acknowledgement Type of funding sources: None.

Age does not necessarily influence latency of event-related potentials

2014 ◽  
Vol 33 (10) ◽  
pp. 723-727
Author(s):  
M. Westermann ◽  
I. W. Husstedt ◽  
A. Okegwo ◽  
S. Evers
Keyword(s):  
Normal Values ◽  
Rank Correlation ◽  
Event Related Potentials ◽  
Intrinsic Property ◽  
Total Sample ◽  
Oddball Paradigm ◽  
Age Dependent ◽  
Related Potentials ◽  
The Mean ◽  
P3 Component

SummaryEvent-related potentials (ERP) are regarded as age dependent. However, it is not known whether this is an intrinsic property of ERP or an extrinsic factor. We designed a setting in which ERP were evoked using a modified oddball paradigm with highly differentiable and detectable target and non-target stimuli. A total of 98 probands were enrolled in this study. We evaluated the latency and amplitude of the P3 component of visually evoked ERP. The mean P3 latency was 294 ± 28 ms and was not related to age (r = –0.089; p = 0.382; Spearman-rank-correlation). The P3 amplitude was related to age in the total sample (r = –0.323; p = 0.001; Spearmanrank-correlation) but not in the probands under the age of 60 years. There were no significant differences regarding sex. Our findings suggest that ERP are not age dependent if highly differentiable and detectable stimuli are used. This should be considered when normal values of ERP are created for clinical use.

HAPTEN-RADIOIMMUNOASSAY OF PLASMA OESTRADIOL

1973 ◽  
Vol 72 (2) ◽  
pp. 330-344 ◽  
Author(s):  
Peter Doerr
Keyword(s):  
Adult Male ◽  
Normal Values ◽  
Normal Adult ◽  
Adult Males ◽  
Reagent Blank ◽  
Keyhole Limpet Haemocyanin ◽  
Ether Extraction ◽  
The Mean ◽  
Assay Precision ◽  
Plasma Oestradiol

ABSTRACT A hapten-radioimmunoassay for plasma oestradiol is described and information about the reliability of the method is given in detail. Oestradiol-3-hemisuccinate coupled to keyhole limpet haemocyanin is used for immunization of rabbits. The antiserum utilized for the assay is characterized by its titer, affinity and specificity. Following ether extraction and NaOH-light petroleum partition oestradiol is separated from crossreacting oestrogens by TLC. Oxidation of oestradiol on the plate is prevented by mercaptoethanol. To separate free and antibody bound ligand 250 μg dextran-coated charcoal per tube is used in the presence of bovine serum gammaglobulin (1 mg/ml). The between-assay precision based on 15 different determinations of control samples from normal adult male plasma was 9.4% (C. V.). The mean reagent blank value of 31 determinations was equivalent to 0.3 pg oestradiol and the detection limit in terms of the 99% confidence limit for a single blank value, was equivalent to 4.3 pg oestradiol. A procedure for detecting plasma blanks is described. Plasma oestradiol is separated from approximately all concomitant substances originally present in the sample by enzymatic conversion into oestrone and a second TLC. No plasma blanks could be detected with respect to normal adult male plasma. Normal values for adult males based on 51 subjects were characterized by a median of 17.2 pg/ml and the 95 percentiles of 9.5–27.6.

Association of Retained Fetal Membranes with Haematological Profile in Crossbred Cows

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
K. Rokde ◽  
S. Kumar ◽  
A. Bhardwaz ◽  
S. S. Mahour ◽  
S. P. Nema ◽  
...  
Keyword(s):  
Eosinophil Count ◽  
Jugular Vein ◽  
Dairy Farm ◽  
Fetal Membranes ◽  
Monocyte Count ◽  
Blood Samples ◽  
Mean Values ◽  
Crossbred Cows ◽  
Haematological Profile ◽  
The Mean

This study was carried out on clinical cases of retained fetal membranes in crossbred cows presented at College Clinics and College dairy farm and from Villages in and around Mhow. The blood samples were collected from jugular vein just before 12 hr. postpartum and on 7th day postpartum. Haematological profile revealed that the mean values of haemoglobin, neutrophil and monocyte count after 12 hrs and 7th day postpartum were significantly lower and lymphocyte count was significantly higher in RFM cows (n=18) than normally calved cows (n=6). The differences in mean TLC, eosinophil and basophil counts were non-significant at 12 hrs postpartum, however on 7th day postpartum the TLC and eosinophil count were significantly higher and basophil count was non-significantly different in RFM cows than the normally calved cows.

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