Association of Retained Fetal Membranes with Haematological Profile in Crossbred Cows

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
K. Rokde ◽  
S. Kumar ◽  
A. Bhardwaz ◽  
S. S. Mahour ◽  
S. P. Nema ◽  
...  
Keyword(s):  
Eosinophil Count ◽  
Jugular Vein ◽  
Dairy Farm ◽  
Fetal Membranes ◽  
Monocyte Count ◽  
Blood Samples ◽  
Mean Values ◽  
Crossbred Cows ◽  
Haematological Profile ◽  
The Mean

This study was carried out on clinical cases of retained fetal membranes in crossbred cows presented at College Clinics and College dairy farm and from Villages in and around Mhow. The blood samples were collected from jugular vein just before 12 hr. postpartum and on 7th day postpartum. Haematological profile revealed that the mean values of haemoglobin, neutrophil and monocyte count after 12 hrs and 7th day postpartum were significantly lower and lymphocyte count was significantly higher in RFM cows (n=18) than normally calved cows (n=6). The differences in mean TLC, eosinophil and basophil counts were non-significant at 12 hrs postpartum, however on 7th day postpartum the TLC and eosinophil count were significantly higher and basophil count was non-significantly different in RFM cows than the normally calved cows.

Clinico-Haematological Alterations in Goats Affected with Ruminal Acidosis

2017 ◽  
Vol 13 (02) ◽  
Author(s):  
P. R. Chavelikar ◽  
G. C. Mandali ◽  
D. M. Patel
Keyword(s):  
Clinical Signs ◽  
Clinical Manifestations ◽  
Nasal Discharge ◽  
Mean Values ◽  
Ruminal Acidosis ◽  
Reduced Body ◽  
Muscle Twitching ◽  
Haematological Profile ◽  
The Mean ◽  
Severity Of The Disease

Ruminal acidosis is one of the most important clinical emergencies in sheep and goats resulting into high mortality rate. In the present study, eight healthy farm goats and 24 goats presented to the TVCC of the college with clinical signs of ruminal acidosis like anorexia, tympany, increased pulse and respiratory rate, reduced body temperature, doughy rumen, enteritis, oliguria, grinding of teeth, purulent nasal discharge, muscle twitching, arched back, dehydration and recumbency with rumen liquor pH below 6 were examined for haematological alterations using autohaematoanalyzer. Among various haematological parameters evaluated from acidotic goats, the mean values of Hb (12.21±0.17 vs. 10.86±0.15 g/dl), TEC (14.28±0.16 vs. 12.04±0.36 ×106/ μl), TLC (13.43±0.11 vs. 11.11±0.27 ×103/μl), PCV (36.91±0.53 vs. 29.88±0.55%), neutrophils (64.54±0.93 vs. 28.13±0.92%), MCV (23.38±0.37 vs. 19.38±1.34 fl) and MCH (7.03±0.08 vs. 6.31±0.25 pg) were found significantly increased, while the mean values of lymphocytes (28.00±0.82 vs. 65.38±0.80%) and MCHC (24.55 ±0.26 vs. 34.88±0.97 g/dl) were decreased significantly from the base values of healthy goats. It was concluded that ruminal acidosis induced due to accidental heavy ingestion of readily fermentable carbohydrate rich grains and food waste significantly altered the haematological profile concurrent with clinical manifestations in goats, and hence can be used to assess the severity of the disease.

scholarly journals Metabolic evaluation of Crioulo horses participating in competitions of 750 km

2013 ◽  
Vol 33 (12) ◽  
pp. 1471-1477 ◽  
Author(s):  
Lorena Alvariza Amaral ◽  
Millie Marchiori ◽  
Charles Ferreira Martins ◽  
Marcio Nunes Correa ◽  
Carlos Eduardo Wayne Nogueira
Keyword(s):  
Inflammatory Process ◽  
Ionized Calcium ◽  
Metabolic Responses ◽  
Total Calcium ◽  
Blood Cell Count ◽  
Metabolic Evaluation ◽  
Sodium Potassium ◽  
Blood Samples ◽  
Mean Values ◽  
The Mean

The Crioulo breed of horses performs in one of the most physically demanding equestrian competitions, the Marcha de Resistência, which is a contest in which the horses run 750 km in 15 days. The study's aim was to characterize the metabolic responses during this period. We evaluated eleven Crioulo horses in the competition, specifically, two males and nine females. Blood samples were collected 24 hours before the contest and on the 4th, 9th, 11th, 14th and 15th days of competition. We evaluated CK, AST, LDH, glucose, lactate, urea, creatinine, sodium, potassium, chloride, magnesium, total calcium, ionized calcium, total protein, hematocrit and the white blood cell count. At the end of the competition, the mean values of serum AST were 1151±358 IU/ L the mean LDH values were 7418±1695 IU/L and CK was 13,867±3998UI /L. There was a significant increase in urea, creatinine and lactate (p<0.0001). A decrease in the mean values of chloride, sodium, potassium, and total and ionized calcium was observed (p≤0.0002). An evaluation of the total leukocytes and segmented neutrophils (p≤0.0002) revealed their increased values, and decreased values were observed for hematocrit, plasma protein and total lymphocytes (p≤0.0003). The values of glucose, on average, remained constant. Based on these data, we conclude that the Marcha de Resistência competition necessitated a high muscular demand and the depletion of energy and electrolytes, suggesting an inflammatory process in the animals evaluated.

Interference of Plasma Fluorophores on the Red Blood Cell Zinc Protoporphyrin : Haemoglobin Ratio as Determined on a Haematofluorimeter

1989 ◽  
Vol 26 (4) ◽  
pp. 368-373 ◽  
Author(s):  
P C Bartels ◽  
P W Helleman ◽  
J B J Soons
Keyword(s):  
Blood Cells ◽  
Extraction Method ◽  
Isotonic Saline ◽  
Zinc Protoporphyrin ◽  
Reference Ranges ◽  
Reference Groups ◽  
Blood Samples ◽  
Mean Values ◽  
Good Relationship ◽  
The Mean

Direct measurement of the zinc protoporphyrin:haemoglobin ratio (ZPP: Hb ratio) in blood samples is performed by using a haematofluorimeter. Interference by non-specific fluorophores can be eliminated by removing the plasma and making the measurement on washed red blood cells (RBCs). After re-suspending RBCs in isotonic saline, haematofluorimeter readings for the ZPP: Hb ratios revealed higher stability in the course of time whereas a good relationship was found with results obtained by application of an extraction method. Separate reference ranges were established for adult male and female subjects. After washing, the mean values calculated for ZPP: Hb ratios of subjects belonging to the reference groups demonstrated a reduction of 0·04 μmol ZPP mol Hb, corresponding with approximately 30%. In the patients' group, application of washing resulted in a variable decrease of ZPP: Hb ratios.

HOURLY FLUCTUATIONS IN THE BLOOD LEVELS OF MELATONIN, PROLACTIN, LUTEINIZING HORMONE, FOLLICLE-STIMULATING HORMONE, TESTOSTERONE, TRI-IODOTHYRONINE, THYROXINE AND CORTISOL IN RAMS UNDER ARTIFICIAL PHOTOPERIODS, AND THE EFFECTS OF CRANIAL SYMPATHECTOMY

1982 ◽  
Vol 92 (2) ◽  
pp. 237-250 ◽  
Author(s):  
G. A. LINCOLN ◽  
O. F. X. ALMEIDA ◽  
H. KLANDORF ◽  
R. A. CUNNINGHAM
Keyword(s):  
Follicle Stimulating Hormone ◽  
Time Of Day ◽  
Blood Levels ◽  
Blood Concentrations ◽  
Blood Samples ◽  
Mean Values ◽  
Change In The Mean ◽  
The Mean ◽  
Cervical Ganglia ◽  
Short Days

Eight adult Soay rams (four control and four cranially sympathectomized by removal of the superior cervical ganglia) were exposed to alternating 16-week periods of short days of 8 h light: 16 h darkness (8L : 16D) and long days (16L : 8D) for more than 3 years, and the changes in the diameter of the testes were recorded. Once during short days and once during long days blood samples were collected hourly for 28 h, and the concentrations of melatonin, prolactin, FSH, LH, testosterone, tri-iodothyronine (T3), thyroxine (T4) and cortisol were measured by radioimmunoassay. In the control rams the testes were reduced in size during long days compared to short days, and the blood concentrations (24-h mean values) of FSH, LH and testosterone were decreased. The levels of prolactin were much increased, while there was no significant change in the mean levels of melatonin, T3, T4 and cortisol. During both photoperiods there was considerable hour-to-hour variation in all eight hormones indicative of episodic secretion, as well as a consistent variation related to the time of day which was most pronounced for melatonin and T3. There was a clear difference in the daily profile of plasma melatonin levels between short and long days. In the superior cervical ganglionectomized rams there were no significant changes in the size of the testes or in the hormone titres between short and long days. Compared to the controls the plasma levels of LH, FSH, testosterone and prolactin were in the intermediate range. Some consistent diurnal variation was evident in the levels of all the hormones measured, with a pattern similar to the controls for a few of the hormones (e.g. T3) but quite different for others (e.g. melatonin).

scholarly journals Comparative evaluation of haematological profile of the Nigerian indigenous and Large White x Landrace crossbred pigs vaccinated with sheep RBC

2021 ◽  
Vol 43 (2) ◽  
pp. 192-201
Author(s):  
F. O Abonyi ◽  
U. C Arinzechukwu ◽  
D. C Eze ◽  
E. C Okwor ◽  
J. I. Eze ◽  
...  
Keyword(s):  
Comparative Evaluation ◽  
Large White ◽  
Mean Values ◽  
Haematological Response ◽  
Animal Management ◽  
Standard Procedures ◽  
Haematological Profile ◽  
The Mean ◽  
Wbc Count ◽  
Crossbred Pigs

This study was designed to evaluate haematological response between Nigerian indigenous pigs (NIP) and Large White x Landrace crossbred pigs (LWL) when vaccinated with sheep RBC. Twenty male pigs with average age of 42 ± 3 days and weighed 3.10 ± 0.29 kg (NIP) and 6.90 ± 0.43 kg (LWL), were used. They were assigned to groups A (10 NIP) and B (10 LWL); each was further divided into two with five animals in each replicate. Processing of sheep RBC followed standard procedures; feed and animal management were similar. All the pigs were vaccinated with1 ml of 10 % sheep RBC per pig at week 4 and revaccinated 3 weeks later. Their haematological profiles were determined using standard procedures and the study lasted ten weeks. There was a rise in WBC count of both groups after initial vaccination. Mean WBC count of LWL was significantly (p ≤ 0.05) higher at weeks 0, 1, 4 and 6, respectively. Lymphocyte count of the two groups also increased sharply after initial vaccination; values recorded for LWL showed a slight but a continuous rise while those of the NIP generally decreased and increased in value. A significant differences in mean values of neutrophil were recorded prior to initial vaccination (p = 0.011, t = 8), on weeks 1 (p = 0.013, t = 8) and 3(p = 0.005, t = 8) with NIP maintaining higher values than LWL. There was no significance (p ≥0.05) difference in the mean values of eosinophil, basophil and monocytes for both groups.

THE INFLUENCE OF GILT AGE ON THE ENDOCRINE AND OVULATORY RESPONSE TO DIFFERENT DOSES OF ESTRADIOL BENZOATE

1988 ◽  
Vol 68 (3) ◽  
pp. 979-982 ◽  
Author(s):  
R. N. KIRKWOOD ◽  
F. X. AHERNE
Keyword(s):  
Body Weight ◽  
Luteinizing Hormone ◽  
Key Words ◽  
Jugular Vein ◽  
Estradiol Benzoate ◽  
Blood Samples ◽  
Estrous Cyclicity ◽  
The Mean ◽  
Different Doses ◽  
Phasic Release

Fifty-five prepubertal Yorkshire × Landrace gilts were randomly assigned to one of six treatments involving the injection (i.m.) of estradiol benzoate (EB) at either 10 or 20 μg kg−1 body weight at gilt ages of 130, 150 or 170 d. Blood samples were taken by jugular vein puncture at 0, 24, and 36 h, and then at 6-h intervals until 84 h, after injection and assayed for plasma luteinizing hormone (LH) concentrations. The mean magnitude of the EB-induced LH peak was 6.1 ± 0.2 ng mL−1, and was not affected by treatment. Fifty-six percent of gilts ovulated in response to EB injection, and 34% had a subsequent ovulation. The initial ovulation rate was unaffected by EB dose, but was higher (P < 0.05) in 170 d (9.9 ± 1.0) compared to younger gilts (4.2 ± 1.1 and 5.5 ± 1.1 for 130-dand 150-d gilts, respectively). Subsequent ovulation rates were unaffected by treatment. It is concluded that EB treatment will usually induce a phasic release of LH from prepubertal gilts, but this release is not necessarily accompanied by normal estrous cyclicity. Key words: Estradiol, gilts, ovulation

Serum cholesterol levels in Hair goats of Aksaray Region

2018 ◽  
Author(s):  
Tahir Karaþahin ◽  
Neþe Hayat Aksoy ◽  
Ali Evren Haydardedeoðlu ◽  
Þükrü Dursun ◽  
Gaye Bulut ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Young Male ◽  
Young Female ◽  
Blood Samples ◽  
Mean Values ◽  
Cholesterol Levels ◽  
Gender And Age ◽  
The Mean ◽  
Sera Samples

This study was conducted to determine the levels of serum total cholesterol in Hair goats in different gender and age ranges. For this purpose, the blood samples from 120 Hair goats, young (£ 6 months) and adult (2-4 years), which were found to be clinically healthy, were taken from the vena jugularis. Triglyceride, total cholesterol, HDL and LDL cholesterol levels were measured in sera samples. The mean values in adult female goats, young female goats, adult male goats and young male goats were found. Triglyceride levels were found to be higher in male goats than in females. It was observed that the HDL values obtained from male kids were lower than the other ones. The LDL results obtained from the goats were close to each other and statistically insignificant. Thus, in this study, the data obtained is considered to contribute to the formation of reference values in the Hair goats.n

APPLICATION OF PIXE TO DETERMINATION OF ELEMENTS IN THE PLACENTA, MATERNAL BLOOD AND CORD BLOOD

1997 ◽  
Vol 07 (03n04) ◽  
pp. 211-217 ◽  
Author(s):  
Yoshito Watanabe ◽  
Yoshie Takubo ◽  
Masae Yukawa ◽  
Yoshikazu Nishimura ◽  
Hitoshi Imaseki ◽  
...  
Keyword(s):  
Cord Blood ◽  
Maternal Blood ◽  
Pixe Analysis ◽  
Blood Samples ◽  
Mean Values ◽  
The Mean ◽  
Standard Deviations ◽  
Homogenize Sample ◽  
Cu And Zn

PIXE technique was applied to the measurement of elements in the placenta, maternal blood and cord blood of human. The elements determined in these samples include Cl , K , Ca , Fe , Cu and Zn . The values obtained by PIXE were compared with those by ICP-AES to test the accuracy of the method. In placental samples, the mean values of K , Fe , Cu and Zn concentrations obtained by the two methods agree, while Ca concentration is lower in PIXE analysis. The values from PIXE, however, show larger variations resulting from inhomogeneity of the placental samples composed of various tissues different in histological functions. In the analysis of blood samples, the results of the two methods agree for Cl , K , Ca , Fe , Cu and Zn , although the standard deviations tend to be larger in PIXE. These results indicate that PIXE is a useful method for the determination of elements in placental and blood samples, although the preparation to homogenize sample is necessary to obtain accurate results.

scholarly journals Effect of Ecbolic Therapy on Postpartum Reproductive Parameters and Serum Hormonal Profile in Dangi Cows

2018 ◽  
Vol 14 (3) ◽  
Author(s):  
C.T. Khasatiya ◽  
D.B. Bhoi ◽  
V.K. Desai
Keyword(s):  
Jugular Vein ◽  
Conception Rate ◽  
Reproductive Parameters ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Service Period ◽  
Increasing Trend ◽  
The Mean ◽  
Postpartum Estrus ◽  
Estradiol 17Β

The study was conducted on eighteen dystocia affected Dangi cows to evaluate the effect of ecbolic therapy on postpartum reproductive parameters as well as serum progesterone and estradiol-17β profile. The cows were randomly divided into three groups, each of 6 animals. The cows in Gr-I and Gr-II were injected with Methyl ergometrine (5 mg) and Dinoprost tromethamine (PGF2α, 25 mg) i/m, respectively, immediately after parturition and the cows of Gr-III received herbal ecbolic boli (2-4 boli, bid, PO) for first 10 days postpartum. The blood samples were collected on 0 day (day of calving), 7th day, 14th day, 21st day and 28th day postpartum from the jugular vein. The mean first postpartum estrus interval in Gr-I, Gr-II and Gr-III were 181.50 ± 44.73 days, 195.33 ± 23.38 days and 155.33 ± 27.81 days. The service period was 237.66 ± 28.01 days with 50 per cent (3/6) conception rate in Gr-I and 402.00 ± 46.12 days and 368.66 ± 53.46 days with 100 per cent (6/6) conception rate in each Gr-II and Gr-III, respectively. The postpartum estrus interval and service period did not differ significantly (p>0.05) between Gr-I, Gr-II and Gr-III. A highly significant (p less than 0.01) decrease in both progesterone and estradiol-17β concentration was observed on 7th day postpartum and thereafter fluctuated non-significantly with increasing trend on 14th , 21st and 28th day postpartum in all groups.

Impact of Subclinical Mastitis on Milk Quality in Different Seasons

2020 ◽  
Keyword(s):  
Chemical Constituents ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Strong Positive Correlation ◽  
Strong Negative Correlation ◽  
Mean Values ◽  
Milk Samples ◽  
Significant Difference ◽  
Different Seasons ◽  
The Mean

This study was carried out to evaluate the influence of subclinical mastitis on chemical constituents of cow’s milk samples in different seasons; which collected from 444 apparently healthy Holstein Frisian dairy animals during the period of December 2016 to November 2017 in a private dairy farm located in Fayoum district, Egypt. The results of chemical analysis of the milk samples along the four seasons of the year which done by the Lactoscan SLC milk analyzer device revealed that; the values of mean for (Fat%, Protein%, SNF%, Lactose%, Salt% and SCC/ml) in winter were (2.22±0.06, 2.90±0.08, 7.79±0.22, 4.14±0.12, 0.72±0.02 and 6.9×105±1.9×104 respectively).While the mean values of the same parameters in spring were (2.72±0.23, 2.58± 0.22, 6.93±0.58, 3.67±0.31, 0.64±0.05 and 9.2×105±7.7×104 respectively). In the summer, the mean values of (Fat%, Protein%, SNF%, Lactose%, Salt% and SCC/ml) were (2.35± 0.24, 2.80 ±0.28, 7.55±0.76, 4.00±0.40, 0.70±0.07 and 7.7×105±7.7×104 respectively), while the mean values in the autumn were (2.89±0.09, 2.69±0.08, 7.25±0.21, 3.84±0.11, 0.67±0.02 and 11.0×105±3.2×104 respectively). The analysis of findings specified that; there is a significant difference (P<0.05) in fat % and SCC between winter and autumn, however there is a significant difference (P<0.05) between (winter and autumn) and (winter and spring) in the parameters of protein, SNF and lactose content. The results reveal that there is a strong negative correlation between SCC and fat %; however there is a strong positive correlation between SCC and salt %.

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