scholarly journals Phenotypic characterization of extended-spectrum-beta‑lactamase producing E. coli from healthy individuals, patients, sewage sludge, cattle, chickens and raw meat

2017 ◽  
Vol 33 (4) ◽  
Author(s):  
Rabia Saleem ◽  
Hasan Ejaz ◽  
Aizza Zafar ◽  
Sonia Younas ◽  
Ahsan Waheed Rathore
Keyword(s):  
Sewage Sludge ◽  
Phenotypic Characterization ◽  
Beta Lactamase ◽  
Healthy Individuals ◽  
Raw Meat ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

scholarly journals Molecular characterization of bla TEM and bla CTX-M in Multi-Drug Resistance E. coli isolated from Country Chicken and Country Chicken Eggs in Poultry Farms of Malaysia

2020 ◽  
Vol 2 (1) ◽  
pp. 78
Keyword(s):  
Multidrug Resistance ◽  
Multidrug Resistant ◽  
Disk Diffusion ◽  
Phenotypic Characterization ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Chicken Eggs

Over the years, multidrug-resistant Escherichia coli has contributed to the development of extended-spectrum beta-lactamase (ESBLs), which evolved primarily from poultry in every corner of the world. The unregulated use of antibiotics commonly administered to poultry products to prevent any subclinical infections that lead to multidrug resistance (MDR) that is due to acquired bacteria resistance. The main aim of this study is to investigate the prevalence in Malaysia of multidrug-resistant, extended-spectrum beta-lactamase (ESBL) producing E.coli from poultry country chicken and country eggs. In several states of Malaysia, fifty samples from country chicken and country chicken eggs were extracted randomly from chosen poultry. The sample was taken from May to June 2019. The samples were tested using traditional microbiological techniques for the presence of E. coli. Antibiotics susceptibility test using 5 forms of β-lactam antibiotics was used using the double-disk diffusion screening, and confirmation of the test is performed by a combination disk diffusion process to establish the strains generating the ESBL. Although, the phenotypic characterization of bla TEM and bla CTX-M ESBL was carried out using PCR and SDS methods. Twenty of the fifty samples collected were classified as E.coli (20/50), suggesting 40%. The results of the distribution of the β-lactamases genes were reported as bla TEM, bla CTX-M with results of 75% (3/4), and 100% (4/4), respectively. The findings indicate a high prevalence of multidrug resistance as the most prevalent of all ESBL genes in ESBL infections with CTX-M genes. Consequently, effective monitoring of MDR infections, in particular resistance to β-lactamases in poultry chicken, can predict the potential for ESBL infections in humans and animals.

scholarly journals Molecular characterization of extended-spectrum beta-lactamase producing Enterobacteriaceae in a Saudi Arabian tertiary hospital

2014 ◽  
Vol 8 (03) ◽  
pp. 282-288 ◽  
Author(s):  
Hoda Hassan ◽  
Baha Abdalhamid
Keyword(s):  
Tertiary Hospital ◽  
Multidrug Resistant ◽  
Beta Lactamase ◽  
M Gene ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Vitek 2 ◽  
High Level

Introduction: The aim of this study was to determine the prevalence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), and Proteus mirabilis (P. mirabilis). In addition, different methods for detection of these enzymes, including the newly introduced CHROMagar ESBL, were evaluated. Methodology: A total of 382 Enterobacteriaceae clinical isolates were obtained from King Fahad Specialist Hospital – Dammam, during 2011 and screened for production of ESBL using advanced expert system of Vitek 2, CHROMagar and ESBL-E-strips. PCR assay was used to detect blaTEM, blaSHV, and blaCTX-M genes. Susceptibility to a panel of antibiotics was determined. Results: The overall proportion of ESBL-producing enterobacterial isolates was 30.6%, which was higher in E. coli (35.8%) than in K. pneumoniae (25.7%). ESBL genotypes showed remarkable increase in the CTX-M (97.4%) compared to SHV (23.1%). The predominant ESBL was CTX-M- 15 (92.1 %). No TEM ESBL was detected in this study. The Vitek2 showed the highest sensitivity (100%), and the CHROMagar had the lowest specificity (97.3%) compared to the molecular method. All isolates were susceptible to imipenem and meropenem. Conclusions: This study confirms a high level of blaCTX-M positive ESBL isolates are circulating in the Eastern Province of Saudi Arabia. The trend of a multidrug-resistant profile associated with the recovery of the blaCTX-M gene is alarming.

scholarly journals The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 28 ◽  
Author(s):  
Leta Elias ◽  
David C. Gillis ◽  
Tanya Gurrola-Rodriguez ◽  
Jeong Ho Jeon ◽  
Jung Hun Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pcr Analysis ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Esbl Gene ◽  
Extended Spectrum ◽  
Carbapenemase Gene ◽  
Type Gene

Escherichia coli isolates were recovered from clinical specimens of equine patients admitted to the Texas A&M Veterinary Medical Teaching Hospital over a five-year period. Ceftiofur resistance was used as a marker for potential extended-spectrum beta-lactamase (ESBL)-activity, and of the 48 ceftiofur-resistant E. coli isolates, 27.08% (n = 13) were phenotypically ESBL-positive. Conventional PCR analysis followed by the large-scalebla Finder multiplex PCR detected the ESBL genes, CTX-M-1 and SHV, in seven out of the 13 isolates. Moreover, beta-lactamase genes of TEM-1-type, BER-type (AmpC), and OXA-type were also identified. Sequencing of these genes resulted in identification of a novel TEM-1-type gene, called blaTEM-233, and a study is currently underway to determine if this gene confers the ESBL phenotype. Furthermore, this report is the first to have found E. coli ST1308 in horses. This subtype, which has been reported in other herbivores, harbored the SHV-type ESBL gene. Finally, one out of 13 E. coli isolates was PCR-positive for the carbapenemase gene, blaIMP-1 despite the lack of phenotypically proven resistance to imipenem. With the identification of novel ESBL gene variant and the demonstrated expansion of E. coli sequence types in equine patients, this study underscores the need for more investigation of equines as reservoirs for ESBL-producing pathogens.

Multidrug Resistance and the Predominance of blaCTX-M in Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae of Animal and Water Origin

2018 ◽  
Vol 28 (4) ◽  
pp. 201-206 ◽  
Author(s):  
Bilel Hassen ◽  
Senda Sghaier ◽  
Mohamed Salah Abbassi ◽  
Mohamed Amine Ferjani ◽  
Meriam Ben Said ◽  
...  
Keyword(s):  
Wastewater Treatment Plants ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Water Origin ◽  
Class 1 ◽  
Synergy Test ◽  
Animal Isolates

The aim of this work was the genetic characterization of cefotaxime-resistant enterobacteria from animals (53 samples), the surface water of rivers (17 samples), and wastewater treatment plants (43 samples) in Tunisia. A total of 48 (42.4%) cefotaxime-resistant isolates were recovered. An extended spectrum beta-lactamase (ESBL) phenotype with a positive double-disk synergy test (DDST) was exhibited by 34 (70.8%) and 14 (29.1%) isolates from water and animal origins, respectively. Isolates from water were identified as: <i>Escherichia coli</i> (<i>n</i> = 17), <i>Hafnia</i> spp<i>.</i> (<i>n</i> = 13), <i>Citrobacter</i> spp<i>.</i> (<i>n</i> = 1), <i>Enterobacter cloacae</i> (<i>n</i> = 1), <i>Klebsiella pneumoniae</i> (<i>n</i> = 1), and <i>K. oxytoca</i> (<i>n</i> = 1). Animal isolates were identified as: <i>E. coli</i> (<i>n</i> = 11), <i>E. cloacae</i> (<i>n</i> = 1), <i>Hafnia</i> spp<i>.</i> (<i>n</i> = 1), and <i>K. pneumoniae</i> (<i>n</i> = 1). PCR investigation of <i>bla</i><sub>CTX-M</sub>, <i>bla</i><sub>TEM</sub>, and <i>bla</i><sub>SHV</sub> genes showed that amongst the 48 isolates with a positive DDST, 41 (87.5%) carried the <i>bla</i><sub>CTX-M</sub> gene, 1 isolate harbored the <i>bla</i><sub>SHV</sub> gene, and 1 isolate coharbored <i>bla</i><sub>CTX-M</sub> with <i>bla</i><sub>SHV</sub> genes. The class 1 and 2 integrons were detected in 27 (56.2%) and 1 (2%) isolates, respectively. Our study showed a significant occurrence of ESBL-producing enterobacteria in animals and aquatic environments with a predominance of <i>bla</i><sub>CTX-M</sub> genes.

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