Bound and Free Phenolics and Its Antioxidant Activity in Grape Pomace

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

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Evaluation of Reducing Ability and Antioxidant Activity of Fruit Pomace Extracts by Spectrophotometric and Electrochemical Methods

Journal of Analytical Methods in Chemistry ◽

10.1155/2020/8869436 ◽

2020 ◽

Vol 2020 ◽

pp. 1-16

Author(s):

Georgii S. Vasyliev ◽

Victoria I. Vorobyova ◽

Olga V. Linyucheva

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Potassium Ferricyanide ◽

Grape Pomace ◽

Black Currant ◽

Ferricyanide Reduction ◽

Electrochemical Testing ◽

Reducing Ability ◽

Fruit Pomace

The component profiles of apricot, grape, and black currant pomace extracts have been analyzed using HPLC coupled to diode-array detection and tandem mass spectrometry (HPLC-DAD-MS). The predominant components in grape, apricot, and black currant pomace extracts were phenolic acids and flavonols. The redox behavior of apricot, black currant, and grape pomace water extracts was evaluated by means of cyclic voltammetry. Also, individual substances mainly present in the extracts were analyzed. The results of electrochemical testing were compared to traditional chemical techniques of potassium ferricyanide reduction (FRAP) and phosphomolybdenum assay, and fair agreement was established. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assays were applied in order to estimate antioxidant activity. The reducing power of the grape extract was found to be higher than that of the apricot and black currant extracts in both potassium ferricyanide reduction (FRAP) and phosphomolybdenum methods.

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Red Grape Pomace Rich in Polyphenols Diet Increases the Antioxidant Status in Key Organs—Kidneys, Liver, and Spleen of Piglets

Animals ◽

10.3390/ani9040149 ◽

2019 ◽

Vol 9 (4) ◽

pp. 149 ◽

Cited By ~ 5

Author(s):

Veronica Sanda Chedea ◽

Laurentiu Mihai Palade ◽

Rodica Stefania Pelmus ◽

Catalin Dragomir ◽

Ionelia Taranu

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Antioxidant Status ◽

Total Antioxidant Status ◽

Good Health ◽

Grape Pomace ◽

Mesenteric Lymph Nodes ◽

Polyphenol Content ◽

Sod Activity ◽

Blood Biochemical

The aim of this work was to evaluate the influence of a diet containing 5% dried GP on the antioxidant status (total antioxidant status (TAS), antioxidant enzyme activity (catalase-CAT, superoxide dismutase-SOD, and gluthatione peroxidase-GPx), and lipid peroxidation) on the key organs of the liver, kidneys, and spleen in relation to health status as indicated by blood biochemical parameters and total polyphenol content in the blood, organs (liver, spleen, kidney, mesenteric lymph nodes, heart, and brain) and Longissimus dorsi muscle in piglets. The GP diet results in a significant increase of TAS in the liver, spleen, and kidneys, with increased CAT activity in the spleen and kidneys, increased SOD activity in the liver, kidneys, and spleen, and increased GPx activity in the kidneys, as well as a decrease in lipid peroxidation in the liver and kidneys. The GP included in the piglets’ feed contained polyphenols that showed antioxidant activity and were absorbed in the plasma, contributing to maintaining the good health of the animals. The inclusion of 5% GP inclusion in the diets of piglets is beneficial for overall normal blood constituent metabolism and helps to maintain piglet health by increasing the polyphenol content in blood plasma and antioxidant activity in the liver, spleen, and kidneys.

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Polyphenol-enriched fractions from Sicilian grape pomace: HPLC–DAD analysis and antioxidant activity

Bioresource Technology ◽

10.1016/j.biortech.2007.10.037 ◽

2008 ◽

Vol 99 (13) ◽

pp. 5960-5966 ◽

Cited By ~ 47

Author(s):

V. Amico ◽

R. Chillemi ◽

S. Mangiafico ◽

C. Spatafora ◽

C. Tringali

Keyword(s):

Antioxidant Activity ◽

Grape Pomace

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RELATIONSHIP BETWEEN INDIGENOUS PROCESSING METHODS OFXYLIA XYLOCARPASEEDS AND THEIR TOTAL FREE PHENOLICS, ANTIOXIDANT ACTIVITY AND HEALTH-RELEVANT FUNCTIONALITY

Journal of Food Biochemistry ◽

10.1111/j.1745-4514.2011.00636.x ◽

2012 ◽

Vol 37 (3) ◽

pp. 343-352

Author(s):

VELLINGIRI VADIVEL ◽

HANS KONRAD BIESALSKI

Keyword(s):

Antioxidant Activity ◽

Processing Methods ◽

Free Phenolics

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Monitoring the antioxidant activity of an eco-friendly processed grape pomace along the storage

Natural Product Research ◽

10.1080/14786419.2020.1815741 ◽

2020 ◽

pp. 1-4 ◽

Cited By ~ 1

Author(s):

Miluska Cisneros-Yupanqui ◽

Anna Zagotto ◽

Angelica Alberton ◽

Anna Lante ◽

Giuseppe Zagotto ◽

...

Keyword(s):

Antioxidant Activity ◽

Grape Pomace

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Polyphenol constituents and antioxidant activity of grape pomace extracts from five Sicilian red grape cultivars

Food Chemistry ◽

10.1016/j.foodchem.2005.09.041 ◽

2007 ◽

Vol 100 (1) ◽

pp. 203-210 ◽

Cited By ~ 148

Author(s):

Giuseppe Ruberto ◽

Agatino Renda ◽

Carmelo Daquino ◽

Vincenzo Amico ◽

Carmela Spatafora ◽

...

Keyword(s):

Antioxidant Activity ◽

Grape Pomace ◽

Red Grape ◽

Grape Cultivars

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Cytoprotection of Antioxidant Biocompounds from Grape Pomace: Further Exfoliant Phytoactive Ingredients for Cosmetic Products

Cosmetics ◽

10.3390/cosmetics5030046 ◽

2018 ◽

Vol 5 (3) ◽

pp. 46 ◽

Cited By ~ 5

Author(s):

Daniela F. Maluf ◽

Melissa Gonçalves ◽

Rebeca D’Angelo ◽

Ana Girassol ◽

Ana Tulio ◽

...

Keyword(s):

Antioxidant Activity ◽

Water System ◽

Grape Pomace ◽

Raw Material ◽

Butylated Hydroxytoluene ◽

Total Phenolic ◽

Acetone Water ◽

Freeze Dried ◽

Dpph Method ◽

Cosmetic Formulations

The goal of this study was to investigate the efficacy and the safety of grape pomace (Vitis labrusca L.) obtained from the winemaking process as an antioxidant raw material for cosmetic formulations. Grape pomace was dried and submitted to extraction with solvents. The extracts were filtered and freeze-dried. The extraction carried out with 75% acetone-water showed a better yield (1.9 g·g−1). High performance liquid chromatography (HPLC) analysis confirmed the presence of ellagic acid as an active biocompound in the extracts. The Total phenolic content of freeze-dried extracts reached 69.83 ± 1.02 mg for 75% acetone-water system. Antioxidant activity determined by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method revealed there was no statistical difference between the extract (EC50 6.9 ± 0.21) and butylated hydroxytoluene (BHT) (EC50 7.6 ± 0.71). Results of cytotoxicity showed that extracts from grape pomace were safe, even at the highest concentration tested (200 mg·mL−1) against the 3T3 cell line. Scanning Electron Microscopy (SEM) analysis revealed that there were no morphological cell changes of cells treated. Cytoprotection of cells towards the oxidation promoted by the peroxide solution was observed in the lowest concentration tested (0.73 mg·mL−1). Grape pomace from the winemaking process revealed a significant antioxidant activity attributed to its biocompounds. The safety and efficacy of extracts of grape pomace as raw material for cosmetic formulations was proposed through cell culture assays.

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