scholarly journals Effect of Cefazolin on endometrial cytology and reagent test strips parameters in bovine endometritis

2021 ◽  
Vol 71 (4) ◽  
pp. 2505
Author(s):  
Τ.Μ. BARAKAT ◽  
G. SHAWKY ◽  
G. ABSY ◽  
M. ABD EL-RAHMAN RAGAB
Keyword(s):  
Dairy Cows ◽  
Treatment Program ◽  
Ph Value ◽  
Saline Group ◽  
Conception Rate ◽  
Test Strips ◽  
Group I ◽  
End Of Treatment ◽  
Subclinical Endometritis ◽  
Endometrial Cytology

Endometritis is one of uterine disorders in dairy cows causing low fertility. This study explored effects of intrauterine (IU) infusion of Cefazolin on endometrial cytology and reagent test strips parameters (leukocyte esterase [LE] and pH) in bovine subclinical endometritis. A total of 90 cows at 49-56 days in milk (DIM) diagnosed for subclinical endometritis were divided randomlyinto three groups, group I (n = 35), treated with IU infusion of 2 grams Cefazolin (Zinol ®, Pharco) diluted with 50 ml saline; group II (n = 28), treated with IU infusion of 2 gramsCephalexin (Ceporex®, GlaxoSmithKline) diluted with 50 ml saline; group III (n = 27), cows kept as untreated control. Cytological examination and reagent test strips were performed in uterine material derived with the cotton swab technique before treatment program and repeated two times later, in 10 days interval. The cows were artificially inseminated at first oestrous after the end of treatment program and conception rates were evaluated. After the end of treatment program, polymorphonuclear cells (PMN) decreased significantly (P <0.05) in Cefazolin and Cephalexin groups (3.23% and 4.39% respectively) compared tocontrol (24.89%) group, also means of LE in these groups became significantly (P<0.05) lower. The Ph value decreased after treatment, this reduction was significant after the first dose of Cefazolin, while in Cephalexin reduction of pH became significant after second dose. Cefazolin had conception rate (77.14%) significantly (P<0.05) higher than cephalexin and control groups (57.14% and 25.93% respectively). Cefazolin and Cephalexin decrease uterine PMN, LE concentration. The pH value after treatment by Cefazolin was lower than in Cephalexin. Moreover, treatment with Cefazolin resulted in a significantly higher conception rate. Thus, Cefazolin is recommended for the treatment of subclinical endometritis in dairy cows.

scholarly journals Determination of thiol/disulphide homeostasis as a new indicator of oxidative stress in dairy cows with subclinical endometritis

2021 ◽  
Vol 91 (2) ◽  
pp. 137-148
Author(s):  
Birten Emre ◽  
◽  
Ömer Korkmaz ◽  
Ismail Koyuncu ◽  
Selim Çomaklı ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dairy Cows ◽  
Immunocytochemical Staining ◽  
Control Group ◽  
Roc Curve Analysis ◽  
Group Iii ◽  
Total Thiol ◽  
Group I ◽  
Subclinical Endometritis

The objective of this study was to determine thiol/disulphide homeostasis (TDH) in infertile cows with subclinical endometritis (SCE). Endometrial cytological samples were collected using a cytobrush to diagnose SCE in 36 infertile cows. According to the results of the cytology examination, those with acute endometritis were classified as Group I (n = 20) and those with chronic endometritis were classified as Group II (n = 16). A control group was formed of heifers as Group III (n = 20). Blood samples were taken from each group on the day of diagnosis (day 0) to analyse TDH. In the cytology examination, both the Giemsa method and immunocytochemical staining were applied to determine chronic inflammation and activity status. In 55.55% (20/36) of the infertile cows with cytological endometritis, the inflammation was determined to be active, and in 44.44% (16/36) it had become chronic. The native thiol and total thiol levels were found to be statistically significantly lower in the acute (206.54 ± 8.30 μmol/L; 227.11 ± 9.30 μmol/L) and chronic SCE cases (225.15 ± 11.89 μmol/L; 247.96 ± 10.80 μmol/L) compared to the heathy control group (308.47 ± 13.59 μmol/L; 336.83 ± 15.5 μmol/L respectively) (P<0.001). Disulphide levels, disulphide/total thiol, native thiol/total thiol and disulphide/native thiol ratios were similar in all the groups (P>0.05). The diagnostic accuracy of native thiol, which can be used in the diagnosis of SCE, was 92.8%, that of total thiol was 89.3% and that of disulphide was 64.3% according to the ROC curve analysis. These results demonstrate that TDH is a reliable and sensitive indicator of oxidative stress in cow SCE, and that abnormal TDH might play a role in SCE pathogenic mechanisms. This is the first study to evaluate thiol/disulphide homeostasis in dairy cows with SCE as a new indicator of oxidative stress.

scholarly journals Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows

2014 ◽  
Vol 97 (1) ◽  
pp. 195-201 ◽  
Author(s):  
L.V. Madoz ◽  
M.J. Giuliodori ◽  
A.L. Migliorisi ◽  
M. Jaureguiberry ◽  
R.L. de la Sota
Keyword(s):  
Dairy Cows ◽  
Grazing Dairy Cows ◽  
Subclinical Endometritis ◽  
Endometrial Cytology

scholarly journals Effect of semen deposition site on conception rate in dairy cows

2008 ◽  
Vol 64 (7) ◽  
pp. 912-914
Author(s):  
Kozdrowski R. ◽  
Dzięcioł M. ◽  
Twardoń J. ◽  
Dejneka G.J.
Keyword(s):  
Dairy Cows ◽  
Significant Role ◽  
Uterine Horn ◽  
Conception Rate ◽  
Group I ◽  
Group Ii ◽  
Uterine Body

The aim of this study was to determine the efficiency of intracornual insemination versus uterine body insemination using a standard insemination dose. All cows have synchronised oestrus with Ovsynch protocol and only cows with strong oestrus signs were inseminated. An experiment was conducted in autumn. In group I 36 cows were inseminated into the uterine body and in group II 33 cows were inseminated into the uterine horn ipsilateral to the ovary with the largest follicle determined by ultrasonography. The conception rate was 69.44% in group I and 69.66% in group II (there were no significant differences). It was concluded that in routine insemination practice the site of semen deposition (into uterine body or into ipsilateral horn) does not play a significant role.

Presence of gammaherpesvirus BoHV-4 in endometrial cytology samples is not associated with subclinical endometritis diagnosed at artificial insemination in dairy cows

2019 ◽  
Vol 229 ◽  
pp. 130-137 ◽  
Author(s):  
Bo Yang ◽  
Osvaldo Bogado Pascottini ◽  
Jiexiong Xie ◽  
Lowiese Desmarets ◽  
Tingting Cui ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Artificial Insemination ◽  
Subclinical Endometritis ◽  
Endometrial Cytology

Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows

2004 ◽  
Vol 62 (1-2) ◽  
pp. 9-23 ◽  
Author(s):  
R Kasimanickam ◽  
T.F Duffield ◽  
R.A Foster ◽  
C.J Gartley ◽  
K.E Leslie ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Subclinical Endometritis ◽  
Endometrial Cytology

Effect of Herbal Galactogogue (Sanjivani biokseera) on Milk Yield and Milk Constituents in Lactating Kankrej Cattle at Organised Farm

2019 ◽  
Vol 15 (02) ◽  
pp. 39-41
Author(s):  
H H Panchasara ◽  
A B Chaudhari ◽  
D A Patel ◽  
Y M Gami ◽  
M P Patel
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Composition ◽  
Nutritional Requirements ◽  
Not Given ◽  
Distinct Effect ◽  
Lactating Dairy Cows ◽  
Group I ◽  
Effect Of Feeding

The study was conducted to evaluate the effect of feeding herbal galactogogue preparation (Sanjivani biokseera) on the milk yield and milk constituents in lactating Kankrej cows. Thirty-two lactating Kankrej cows in their 1st to 6th lactation were taken for the experiment from 3 days after calving up to 52 days postpartum. All the animals were fed as per the standard seasonally available roughages and concentrates to meet their nutritional requirements. The cows were randomly divided into two uniform groups of 16 cows in each according to initial milk yield and milk composition. The animals in group-I were not given any supplement and served as control. The animals in group-II were given Sanjivani biokseera (Naturewell Industries) @ 60 g per day for 1-month, commencing 3 days after calving, in addition to the usual feeds/fodders. A clear difference was observed in milk yield from day 8 onward of experiment between groups with significant (plessthan0 0.05) higher values from day 16-52 in cows fed herbal galactogogue as compared to control, but no such distinct effect on milk constituents was observed on day 52 when analyzed. The use of herbal galactogogue significantly (p lessthan 0.05) increased the overall average of 52 days milk production, which was 9.34 ± 0.21 lit/day in supplemented as compared to 7.75 ± 0.26 lit/day in control animals. It was concluded that herbal galactogogue (Sanjivani biokseera) could increase milk yield in lactating dairy cows through its galactopoetic property and improved rumen environment.

Efficacy of Ovsynch and Ovsynch Plus Protocol for Improvement of Fertility in Postpartum Sahiwal Cows

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
J. P. Lakher ◽  
M. K. Awasthi ◽  
J. R. Khan ◽  
M. R. Poyam
Keyword(s):  
Fixed Time ◽  
Conception Rate ◽  
Control Group ◽  
Group Iii ◽  
Treatment Groups ◽  
Group I ◽  
Early Postpartum ◽  
The Status ◽  
Sahiwal Cows ◽  
And Control

The present study was conducted to investigate the efficacy of Ovsynch and Ovsynch plus protocol in postpartum (day 60) Sahiwal cows (n=18). Animals were randomly divided into three equal groups, viz., Ovsynch group Ovsynch plus group and Control group. Animals of group I (n = 6) were treated with traditional Ovsynch protocol. The animals (n = 6) of group II were treated with Ovsynch plus protocol which consisted of an initial intramuscular injection of eCG (Folligon) @ 250 IU on day 60 postpartum followed 3 days later by GPG (Ovsynch) protocol. In group-III Control, no treatment was given to animals (n = 6). Treated animals were inseminated at a fixed time between 14 and 20 hrs after second GnRH injection, irrespective of estrus detection. Blood samples were collected from each animal on days 50 and 60 postpartum to determine the status of cyclicity in animals based on serum concentrations of progesterone (P4). A third blood sample was collected on the day of prostaglandin treatment to determine the response of first GnRH injection. Four animals each were cyclic, and two were acyclic in both treatment groups. Four animals each responded to first GnRH treatment in both treatment groups. Similarly, two animals each got conceived giving conception rate of 50% (2/4) in each treatment. In the control group, one out of 6 animals got conceived yielding 16.66 % conception rate (1/6) during the study period. It may be thus concluded that Ovsynch and Ovsynch plus protocol may be used during the early postpartum period to improve the reproductive efficiency in postpartum Sahiwal cows.

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