Efficacy of Ovsynch and Ovsynch Plus Protocol for Improvement of Fertility in Postpartum Sahiwal Cows

Author(s):  
J. P. Lakher ◽  
M. K. Awasthi ◽  
J. R. Khan ◽  
M. R. Poyam

The present study was conducted to investigate the efficacy of Ovsynch and Ovsynch plus protocol in postpartum (day 60) Sahiwal cows (n=18). Animals were randomly divided into three equal groups, viz., Ovsynch group Ovsynch plus group and Control group. Animals of group I (n = 6) were treated with traditional Ovsynch protocol. The animals (n = 6) of group II were treated with Ovsynch plus protocol which consisted of an initial intramuscular injection of eCG (Folligon) @ 250 IU on day 60 postpartum followed 3 days later by GPG (Ovsynch) protocol. In group-III Control, no treatment was given to animals (n = 6). Treated animals were inseminated at a fixed time between 14 and 20 hrs after second GnRH injection, irrespective of estrus detection. Blood samples were collected from each animal on days 50 and 60 postpartum to determine the status of cyclicity in animals based on serum concentrations of progesterone (P4). A third blood sample was collected on the day of prostaglandin treatment to determine the response of first GnRH injection. Four animals each were cyclic, and two were acyclic in both treatment groups. Four animals each responded to first GnRH treatment in both treatment groups. Similarly, two animals each got conceived giving conception rate of 50% (2/4) in each treatment. In the control group, one out of 6 animals got conceived yielding 16.66 % conception rate (1/6) during the study period. It may be thus concluded that Ovsynch and Ovsynch plus protocol may be used during the early postpartum period to improve the reproductive efficiency in postpartum Sahiwal cows.

Author(s):  
C. T. Khasatiya ◽  
H. R. Savani ◽  
Swati Saxena ◽  
M. D. Patel

The study was undertaken to find out the estrus induction and conception rate in 18 postpartum anestrus Surti buffaloes divided equally into three groups and treated with Ovsynch protocol alone and in combination with PRID, keeping one group as control. The use of Ovsynch alone and Ovsynch + PRID protocols with fixed-time artificial insemination resulted in 100% estrus induction with 66.66% and 50.00% conception rate at induced estrus, respectively, and the overall conception rate was 83.33% in both the groups. In untreated anestrus control (n=6), only four (66.66%) buffaloes exhibited spontaneous estrus within 120 days of follow-up and conceived giving the first service and overall conception rate as 75.00% and 66.66%, respectively. The mean number of services per conception did not differ significantly among the treatment and control groups. The service periods of buffaloes under both the treatment groups were significantly (p less than 0.05) shorter (57.00±2.81 and 61.20±3.11 days) than the control group (104.00±20.22 days). Thus, the Ovsynch and Ovsynch + PRID protocol can be successfully used to induce fertile estrus in postpartum anestrus buffaloes.


2017 ◽  
Vol 2 (01) ◽  
pp. 43-46
Author(s):  
Dinesh Mahto

A total of 50 cross bred repeat breeder cattle were selected and grouped into 4 treatment and one control group which belonging to organized farms around, K. V. K. Gandhar, Jehanabad. Hydroxyprogesterone caproate (500 mg) was administered I.M. on day 4 post A. I. in group I, In group II, 500mg progesterone was administered I M on day 4 and again on day 10. Evatocin (40 I.U.) was administered I.M. 5 minutes before A.I. to animals belonging to group III. The same drug was used through Intra vulvo-submucosal route (15 I.U.) to animals of group IV. Group V animals received no treatment and served as control. Pregnancy diagnosis was done by rectal palpation D 45-60 after artificial insemination. In group I, the conception rate was found to be 60% as against 20 % in the control (Non-significant), Gr II, the conception rate improved from 20% in control animals to 70% in treated animals (Significant) and Gr III, the C.R. 60% than control(20%) where as Gr IV, C.R. 40% . The difference in conception rate of treatment and control group animals was non-significant.


2021 ◽  
Vol 9 (3) ◽  
pp. 87-90
Author(s):  
A. Jyoth ◽  

The effect of fiber rich product on child hood obesity studied with 60 sample which were collected randomly among 10-12 years and categorized into 2 groups i.e, experimental group and control group. Experimental group further categorized into three groups i.e, supplementation with exercise (n=15), only supplementation group (n=15), only exercise group (n=15) and control group consists of (n=15).Anthropometric, and diet survey conducted as parameters. A fiber rich product was prepared and supplemented for 2 months to the selected subjects and it consists of whole Bengal gram, whole green gram, green peas, barley and jaggery. Positive results were obtained in three experimental groups. Significant decrease observed inweight, and BMI, Total cholesterol, Triglycerides, LDL, VLDL significant increase observed in HDL in group I II and III. The results were (51.60-48.26kg, 24.7-23.1, 195.2-152.3 mg/dl, 168.2-145.0 mg/dl, 52.2-54.13 mg/dl, 109.4- 69.4mg/dl, 33.6-28.7mg/dl) in group I, In group II the results are (50.3-49.86kg, 23.7-23.4, 168.6-161.0mg/dl, 145.4-129.6mg/dl, 44.2-45.2 mg/dl, 95.3-90.0mg/dl, 29.1-28.3mg/dl).In group III the tesults aere (50.7-49.6kg, 24.5-23.9, 143.2-139.3mg/dl, 139-134.5mg/dl, 38.2-38.7mg/dl 76.8-74.1 mg/dl, 25.6-28.1mg/dl) .From the results it was clear that when compared to group II and III group I has shown better results.


2020 ◽  
Vol 5 (3) ◽  
pp. 92-97
Author(s):  
Siska ◽  
Diene Roufiani ◽  
Ema Dewanti

Anaphylaxis is the most common allergic reaction triggered by allergens such as insect poisons, food, and drugs through skin contact, injection, or inhalation. In vitro previous research showed that strawberries fruit have activity as antioxidant, anticancer, anti-inflammation, and anti-allergic. The research aimed to determine the antianaphylaxis strawberry fruit extract in mice (Balb/C strain) with ovalbumin-induced.  Twenty-four Balb/C strain mice were divided into six groups (n=4). Group I and II as a normal and control group. Group III till VI as a treatment group was given cetirizine dose 0.042 mg/20 g BW and strawberry extract doses 0,68; 1,36; and 2,72 mg/20 g BW, respectively. This research showed that 70 % of ethanol extract of strawberries fruit have antiallergic activity in response to active cutaneous anaphylaxis. 70% ethanol extract of strawberries doses 2.72 mg/20 g BW had similar antiallergic activity compare with cetirizine. The conclusion of this study showed that strawberries fruit extract could be developed as an alternative medicine to anti-anaphylaxis or anti-allergic.


2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Steviany Towoliu

Abstract: E. coli is part of the normal flora of the human and animal intestine and is commonly non pathogenic. However, one of the serotypes of this bacteria, which is enteropathogenic E. coli (EPEC), can cause primary infection on the intestine such diarrhea.  The growth of pathogenic bacteria in diarrheal patients can be inhibited by Lactobacillus.  Lactobacillus can function as probiotic which can affect the immune system of the digestive canals.  In addition, Lactobacillus also produce mucus  which can act as barrier to the pathogens.  The objective of this study was to reveal the effects of the administration of Lactobacillus on the microscopic features of the mucosa of the intestine of wistar rats infected by Escherichia coli.  This study was a laboratory experimental research employing 16 wistar rats divided into the control group (4 rats) and three treatment groups (12 rats) consisting of 4 rats each.  Results showed that; in group I the histological features were normal, in group II part of the epithelium of the mucosa showed erosion, dilatation of the capillary vessels, and many lymphosites were observed, in group III the epithelium of the mucosa was intact and the number of lymphosites was liitle, and in group IV the surface of the epithelium was intact, the presence of cell regeneration indicated by the increase number of goblet cells and a small number of  lymphocyes. Conclusions: The administration of after infection by E. coli has benefial effects indicated by the improvement of epithelial cells and the absence of denudation of the epithelium of the intestine. Keywords: E.coli, Lactobacillus, intestinal mucosa.   Abstrak: E.coli merupakan flora normal usus halus manusia dan hewan umumnya tidak menyebabkan penyakit.  Namun salah satu serotipe E.coli yaitu E.coli Enteropatogenik (EPEK) bersifat patogen dan dapat menyebabkan infeksi primer pada usus misalnya diare.  Pertumbuhan bakteri patogen pada pasien diare dapat dihambat oleh Lactobacillus. Lactobacillus merupakan probiotik yang akan mempengaruhi sistem imun saluran cerna serta memproduksi mukus yang berfungsi sebagai penghalang saluran cerna terhadap bakteri patogen.  Penelitian ini bertujuan untuk melihat efek pemberian Lactobacillus terhadap gambaran mikroskopis mukosa usus halus tikus wistar yang diinfeksi dengan Escherichia coli.  Penelitian ini merupakan penelitian eksperimental laboratorik.  Subjek penelitian terdiri dari 16 ekor tikur wistar yang dibagi dalam kelompok kontrol (4 ekor) dan kelompok  perlakuan (12 ekor) dibagi dalam 3 kelompok masing-masing 4 ekor.  Hasil penelitian kelompok I dengan gambaran histologik jaringan usus normal, kelompok II sebagian epitel mukosa usus halus terlihat erosi,  ada pelebaran pembuluh darah kapiler, dan banyak limfosit, kelompok III dengan permukaan epitel mukosa yang utuh dan jumlah limfosit sedikit, dan kelompok IV dengan permukaan epitel yang utuh, adanya regenerasi sel ditandai dengan bertambahnya sel goblet, dan sedikit limfosit.  Simpulan: Pemberian Lactobacillus setelah diberikan E.coli memberi efek yang baik berupa perbaikan sel epitel permukaan dan tidak terlihat denudasi epitel permukaan usus halus. Kata Kunci: E.coli, Lactobacillus, mukosa usus halus.


2017 ◽  
Vol 2 (01) ◽  
pp. 103-109
Author(s):  
Dinesh Mahto

Gastrointestinal parasitic infected ewes and does were kept almost parasite free for about 3 months using Albendazole alone or supplemented with mineral mixture. The Hb (g/dl) level ranged from 8.83±0.08 to 8.96±0.03 in ewes and does was 8.78±0.02 to 8.95±0.24 before treatment. The level of Hb increased significantly (P less than 0.01) in both the treatment groups after treatment on day 20.The Serum level of Ca, P, Cu and Zn in ewes and does increased significantly after treatment on day 20 in both the treatment groups as comparison to control group. Although they did not vary significantly among themselves except the level of Cu which was significantly (P less than 0.01) higher in group II than the group I in ewes and does. The level of Zn was higher in group I than group II in does. The minimum interval from treatment to onset of estrus were recorded in group II , 41 days in ewes and 43 days in does which was followed by group I 44 days in ewes and 46 days in does and control group 55 days in ewes and 52 days in does. The average required number of services per conception in does were 1.33, 1.00 and 2.00 for I, II and control groups, respectively. The corresponding values in ewes were 1.67, 1.17 and 2.17, respectively. The pregnancy rates in the three groups were 66.66, 83.33 and 33.33%, respectively in case of does. The corresponding values in ewes were 50.00, 66.66 and 33.36%, respectively.


2017 ◽  
Vol 32 (2) ◽  
pp. 60 ◽  
Author(s):  
Mailza Costa De Almeida ◽  
Luiz Carlos De Lima Ferreira ◽  
Gisely Naura Venâncio ◽  
Risonilce Fernandes Silva De Souza ◽  
Emerson Silva Lima ◽  
...  

OBJECTIVE: The aim of this study was to histopathologically analyze, in subcutaneous connective tissue in rats, a propolis solution for cavity cleansing and its toxicity through hemolytic and Artemia franciscana tests.METHODS: Fifteen male rats were selected and randomly distributed in three experimental periods (07, 30 and 45 days), in which each animal received the four treatment groups in rounds: Group I – Propolis I; Group II – Propolis II; Group III – Calcium Hydroxide Water and Group IV – 2% Chlorexidine; the sides of the tube were the control group. The data were analyzed using descriptive statistics. The results showed, in terms of biocompatibility, that all materials presented a significant reduction of the inflammatory infiltrate and an increase of the thickness in the collagen fibers. It may be suggested, in decreasing order of biocompatibility, the use of following materials: calcium hydroxide-water, 2% chlorexidine, propolis I and propolis II.RESULTS: In the cytotoxicity test using A. franciscana, the propolis extract showed high toxicity when tested at concentrations and in the hemolytic activity test the propolis I extract showed greater activity than propolis II.CONCLUSION: The present study suggests the use of propolis as a cavity cleansing solution for shallow and medium cavities similar to 2% chlorexidine.


2017 ◽  
Vol 8 (2) ◽  
pp. 61-65
Author(s):  
Arati C Koregol ◽  
TJ Jerin Mary ◽  
Shivaraj Warad ◽  
Tejaswini Annam ◽  
Nitesh Kataria ◽  
...  

ABSTRACT Introduction Micronutrients have been extensively studied in recent years; of these magnesium and iron are essential for a variety of physiologic functions. Activation of neutrophils is an early effect of hypomagnesemia, which, in turn, causes periodontal tissue destruction. Since magnesium also influences blood pressure regulation by vascular tone and reactivity, its altered levels could contribute to the pathophysiology of hypertension. Iron plays a key role in the formation of reactive oxygen species that causes peroxidative damage to tissues. Aims and objectives The present study is designed to estimate the levels of serum iron and magnesium in healthy, chronic periodontitis patients with and without hypertension. Materials and methods This study includes 90 subjects, age ranging from 18 to 65 years. They were categorized into three groups as group I: 30 periodontally healthy, group II: 30 chronic periodontitis, and group III: 30 chronic periodontitis with hypertension. Serum magnesium levels were determined using the modified xylidyl blue reaction method and serum iron levels using ferrozine method without deproteinization via photometry. Results Biochemical parameters showed mean serum iron levels in chronic periodontitis (59.17) to be significantly reduced as compared with the control group (76.90) and slightly increased in the chronic periodontitis with hypertension group (69.63). Serum magnesium levels were found to be significantly lower in the hypertensive group as compared with the chronic periodontitis and control groups. Conclusion Iron and magnesium have a link in the pathophysiology of periodontitis. Serum iron and serum magnesium levels were closely linked to periodontitis and hypertension. How to cite this article Kalburgi NB, Koregol AC, Mary TJJ, Warad S, Annam T, Kataria N. Associating Serum Iron and Magnesium Levels in Hypertensive and Chronic Periodontitis Patients: Do They have a Link? J Health Sci Res 2017;8(2):61-65.


2020 ◽  
pp. 1-3
Author(s):  
Semmalar. R ◽  
Jain Vanitha. N. S ◽  
Fabiola M. Dhanraj ◽  
Anitha Rajendra Babu ◽  
Susila Susila

An experimental post test only control design was used to assess the effectiveness of selected interventions on pain perception and parental satisfaction among infants receiving immunizations at Public Health Centre, Chennai. Using simple random sampling method, the total of 40 infants were allocated in four groups where each group had 10 infants. The interventions provided are oral sucrose for Experimental group I, 4 S’s technique for experimental group II, oral sucrose with 4 S’s technique for Experimental group III and routine care for Control group. The data collection tool consists of demographic profile, modified riley pain scale and parental satisfaction likert scale were used to collect data. The result of the study revealed that mean pain perception of experimental group III (3.21) was less than the experimental group I (4.08), experimental group II (3.40) and control group (4.22). There was no statistical difference between experimental group II and experimental group III at the level of 0.693 and also between experimental group I and control group at the level of 1.000 when other groups had statistically significant difference in pain perception. In mean parental satisfaction, the experimental group III (43.20) had high parental satisfaction than the experimental group I (39.80), experimental group II (41.90) and control group (38.60).Hence the pilot study concluded that 4S’s technique was the effective intervention for infant immunizations.


Author(s):  
Deepak J ◽  
C. Jayakumar, Hiron M. Harshan and K. D. John Martin M. P. ◽  
Hiron M. Harshan and K. D. John Martin C. ◽  
John Martin K. D. ◽  
John Martin K. D.

A study was conducted in 18 healthy, fertile, non-pregnant, dioestrous female dogs to evaluate the efficacy of PGF2? analogue (cloprostenol sodium) in shortening the dioestrus. Bitches that were diagnosed non-pregnant by trans-abdominal ultrasonography, on day 30 of breeding and their dioestrual stage confirmed by serum progesterone assay, were randomly allotted to three groups of six bitches each. Group I and II bitches were treated with cloprostenol sodium @ 2.5 µg/kg b. wt., subcutaneously, thrice at 48 h interval, beginning on day 30 and 45 of last breeding, respectively. Group III animals were assigned as control and were left untreated. Fifty days from first injection of cloprostenol, serum progesterone assay was carried out among the animals in Group I and II whereas the same was done among Group III bitches on day 90 after last breeding. Among Group I and II, mean serum progesterone concentrations were 0.93 ± 0.04 and 0.87 ± 0.01ng/ mL, respectively whereas the level was 1.72 ± 0.9 ng/mL among control group; highly significant difference (p<0.01) existed between control and treatment groups. The present study indicated that administration of PGF2? analogue during dioestrus enhances the pace of luteal regression among non-pregnant bitches.


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