scholarly journals Prevalence and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry

2018 ◽  
Vol 6 (2) ◽  
pp. 96-102 ◽  
Author(s):  
Zohreh Mashak
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Raw Meat ◽  
Meat Samples

scholarly journals Isolation, Identification and Antibiotic Susceptibility of Escherichia coli Isolated from Raw Meat from Modake and Ile-Ife, Osun State, Nigeria

2021 ◽  
pp. 9-13
Author(s):  
Wilkie Eunice Damilola ◽  
Oluduro Anthonia Olufunke ◽  
Ezeani Chidinma Vivian ◽  
Sotala Toyosi Teniola
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antibiotic Susceptibility ◽  
Multiple Antibiotic Resistance ◽  
Raw Meat ◽  
E Coli ◽  
Antibiotic Susceptibility Test ◽  
Mueller Hinton Agar ◽  
Meat Samples ◽  
High Level

The study reported isolation, identification and antibiotic susceptibility of Escherichia coli isolated from raw meat from Modakeke and Ile-ife, Osun State, Nigeria, with the view to determining the antibiogram profiling of the bacterial isolates.  In this study, five samples of fresh meat were collected from different abattoirs in Ile-Ife and Modakeke, Osun State. Isolates of Escherichia coli were isolated, identified morphologically based on their growth on nutrient agar and subjected to antibiotic susceptibility test on Mueller Hinton agar. The mean microbial load from the meat samples ranged from 8.85 x 102cfu/ml to 5.77 x 104cfu/ml. A total of 69 E. coli isolates were recovered from the meat sampled. All the isolates appeared cream, translucent, entire, convex, circular, smooth and glistering. The isolates were identified as Gram negative rods, non-motile, lactose fermenters, positive for indole test and negative for citrate utilization test. All the E. coli isolates were resistant to augmentin, ceftriazone, nitrofurantoin and gentamycin. 98.55% of E. coli isolated was resistant to amoxillin and the least resistant was recorded in ofloxacin (8.70%). However, 91.30% of the E. coli isolates was sensitive to ofloxacin, 81.16% to ciprofloxacin and 36.23% to pefloxacin while none was sensitive to augmentin, ceftriazone, nitrofurantoin and gentamycin. A total of 19 different multiple antibiotic resistance patterns were observed among the isolates. Thirty isolates (43.48%) showed multiple antibiotic resistance to 5 and 10 different antibiotic types each. The study concluded that occurrence of E. coli infection is high in the study area with high level of multiple antibiotic resistance.

Occurrence and Survival of Verocytotoxin-Producing Escherichia coli O157 in Meats Obtained from Retail Outlets in The Netherlands

1999 ◽  
Vol 62 (10) ◽  
pp. 1115-1122 ◽  
Author(s):  
A. E. HEUVELINK ◽  
J. T. M. ZWARTKRUIS-NAHUIS ◽  
R. R. BEUMER ◽  
D E. de BOER
Keyword(s):  
Escherichia Coli ◽  
The Netherlands ◽  
Meat Products ◽  
Storage Period ◽  
Processed Meat ◽  
Raw Meat ◽  
Pork Products ◽  
Minced Beef ◽  
Beef Products ◽  
Meat Samples

In 1996 and 1997, 2,941 fresh and processed meat products obtained from supermarkets and butcher shops in The Netherlands were examined for the presence of verocytotoxin-producing Escherichia coli of serogroup O157 (O157 VTEC). Additionally, the fate of O157 VTEC in raw meat products stored at low temperatures and the effect of different additives were evaluated. O157 VTEC strains were isolated from 6 (1.1%) of 571 samples of raw minced beef, 2 (0.5%) of 402 samples of raw minced mixed beef and pork, 1 (1.3%) of 76 samples of raw minced pork, 1 (0.3%) of 393 samples of other raw pork products, and 1 (0.3%) of 328 samples of cooked or fermented ready-to-eat meats. Other raw beef products (n = 223) and meat samples originating from poultry (n = 819), sheep or lamb (n = 46), or wild animals (n = 83) were all found to be negative for O157 VTEC. For the survival experiments we used tartaar (minced beef with a fat content of less than 10%) and filet americain (tartaar mixed with a mayonnaise-based sauce [80 to 20%]). The O157 VTEC strain tested was able to survive in tartaar and filet americain stored at −20, 0, 5, or 7°C for 3 days. At both 7 and at 15°C, O157 VTEC counts in tartaar and filet americain remained virtually unchanged throughout a storage period of 5 days. Addition of acetic acid (to pH 4.0), sodium lactate (1 and 2% [wt/wt]), or components of the lactoperoxidase–thiocyanate–hydrogen peroxide system to filet americain did not result in a reduction of viable O157 VTEC cells during storage at 7 or 15°C. It was concluded that raw meat contaminated with O157 VTEC will remain a hazard even if the meat is held at low or freezing temperatures.

Occurrence and Characterization of Escherichia coli O157 and Other Serotypes in Raw Meat Products in Morocco

2008 ◽  
Vol 71 (10) ◽  
pp. 2082-2086 ◽  
Author(s):  
LUCIANO BENEDUCE ◽  
GIUSEPPE SPANO ◽  
ARI Q. NABI ◽  
FRANCESCO LAMACCHIA ◽  
SALVATORE MASSA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Susceptibility Testing ◽  
Meat Products ◽  
Molecular Techniques ◽  
Escherichia Coli O157 ◽  
Raw Meat ◽  
E Coli ◽  
Meat Samples ◽  
The City

In this study, 100 raw meat samples were collected from 15 local Moroccan butcheries in five different areas of the city of Rabat during a period of 4 months. Overall, 7 of 15 butcheries from three areas of the city yielded strains of Escherichia coli O157. Single isolates from 9 (9%) of 100 raw meat samples were biochemically and serologically confirmed as E. coli O157. Using molecular techniques, two strains were positive for the Shiga toxin, with two additional strains containing an attaching-effacing gene. All potentially virulent serotypes isolated from these meat samples showed distinct pulsed-field gel electrophoresis profiles. Based on antibiotic susceptibility testing, more than 70% of the isolates were resistant to ampicillin and clavulanic acid–amoxicillin. Moreover, one strain was resistant to more than three antibiotics. Our study represents the first survey of E. coli O157 and related serotypes in raw meat products in Morocco.

scholarly journals Antibiotic Resistance in Escherichia coli and Staphylococcus aureus from Retail Chicken Meat in Surabaya, Indonesia

2020 ◽  
Vol 3 (2) ◽  
pp. 80
Author(s):  
Annisa Aulia Maghfirani Sudarmadi ◽  
Subur Prajitno ◽  
Agung Dwi Wahyu Widodo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Growth Promotion ◽  
Infectious Agent ◽  
Sampling Technique ◽  
Chicken Meat ◽  
Meat Sample ◽  
Meat Samples ◽  
And Staphylococcus Aureus

Introduction: Antimicrobial resistance is becoming a problem in public health. Zoonotic food-borne bacteria is infectious agent that can be transferred from animal to human through the food-producing animal we consume. Nowadays, antibiotic used for human and animal is not only to cure infection but also to aim animal’s growth promotion. It is known as non therapeutic antimicrobial agent (NTA) leading to antibiotic resistance. The third generation cephalosporins, cefotaxime, and also cefoxitin are included as important antibiotic for human. This study aims to identify the presence of cefotaxime-resistant Escherichia coli and cefoxitin-resistant Staphylococcus aureus isolated from chicken meat of both traditional and modern market in Surabaya.Methods: This is descriptive post test only experimental research. We used 8 samples of chicken meat from 4 different market using purposive sampling technique. We cultured Escherichia coli and Staphylococcus aureus from the chicken meat. Sensitivity test was done using Kirby-bauer disk-diffusion method.Results: All chicken meat sample bought from traditional market in Surabaya are contaminated by cefotaxime-sensitive Escherichia coli (n=4/4) while chicken meat sample bought from modern market are not contaminated by Escherichia coli (n=0/4). All chicken meat sample bought from traditional (n=4/4) are also contaminated by cefoxitin-sensitive Staphylococcus aureus. Half of chicken meat sample bought from modern market (n=2/4) are contaminated by cefoxitin-sensitive Staphylococcus aureus, while the other half (n=2/4) are contaminated by cefoxitin-resistant Staphylococcus aureus.Conclusion: Antibiotic resistance is found and all chicken meat samples have been highly contaminated with bacteria therefore food-processing should be done correctly.

scholarly journals Prevalence and Loads of Fecal Pollution Indicators and the Antibiotic Resistance Phenotypes of Escherichia coli in Raw Minced Beef in Lebanon

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1543 ◽  
Author(s):  
Issmat I. Kassem ◽  
Nivin A Nasser ◽  
Joanna Salibi
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Food Safety ◽  
Food Systems ◽  
Meat Products ◽  
Fecal Coliforms ◽  
E Coli ◽  
Beef Meat ◽  
Minced Beef ◽  
Meat Samples

Meat is an important source of high biological value proteins as well as many vitamins and minerals. In Lebanon, beef meats, including raw minced beef, are among the most consumed of the meat products. However, minced beef meat can also be an important source of foodborne illnesses. This is of a major concern, because food safety in Lebanon suffers from well-documented challenges. Consequently, the prevalence and loads of fecal coliforms and Escherichia coli were quantified to assess the microbiological acceptability of minced beef meat in Lebanon. Additionally, antibiotic resistance phenotypes of the E. coli were determined in response to concerns about the emergence of resistance in food matrices in Lebanon. A total of 50 meat samples and 120 E. coli isolates were analyzed. Results showed that 98% and 76% of meat samples harbored fecal coliforms and E. coli above the microbial acceptance level, respectively. All E. coli were resistant to at least one antibiotic, while 35% of the isolates were multidrug-resistant (MDR). The results suggest that Lebanon needs to (1) update food safety systems to track and reduce the levels of potential contamination in important foods and (2) implement programs to control the proliferation of antimicrobial resistance in food systems.

Prevalence of Campylobacter, Salmonella, and Escherichia coli on the External Packaging of Raw Meat

2005 ◽  
Vol 68 (3) ◽  
pp. 469-475 ◽  
Author(s):  
F. BURGESS ◽  
C. L. LITTLE ◽  
G. ALLEN ◽  
K. WILLIAMSON ◽  
R. T. MITCHELL
Keyword(s):  
Escherichia Coli ◽  
External Surface ◽  
Hazard Analysis ◽  
Campylobacter Coli ◽  
Microbiological Contamination ◽  
Antimicrobial Drugs ◽  
Raw Meat ◽  
E Coli ◽  
Meat Samples ◽  
Raw Meats

During September and October 2002, 3,662 prepackaged raw meat samples were collected to evaluate the extent and nature of microbiological contamination on external surfaces of the packaging, which could potentially cross-contaminate ready-to-eat foods during and after purchase. Salmonella was detected on two (<1%) samples of external packaging (both from raw chicken), and Campylobacter was detected on 41 (1.1%) samples of external packaging. The external packaging of game fowl exhibited the highest Campylobacter contamination (3.6%), followed by raw chicken (3.0%), lamb (1.6%), turkey (0.8%), pork (0.2%), and beef (0.1%); Campylobacter jejuni and Campylobacter coli accounted for 59% (24 of 41) and 24% (10 of 41) of the contaminating Campylobacter species, respectively. C. coli isolates from the external packaging were more multiresistant to antimicrobial drugs, including quinolones such as ciprofloxacin, than was C. jejuni. Escherichia coli (an indicator of fecal contamination) was isolated from the external packaging on 4% of the raw meat samples at levels of 40 to 105 CFU per swab. The external packaging of raw meats is a vehicle for potential cross-contamination by Campylobacter, Salmonella, and E. coli in retail premises and consumers' homes. The external surface of heat-sealed packaging was less frequently contaminated with Campylobacter and E. coli compared with other types of packaging (e.g., overwrapping, bag, and tie tape) (P < 0.0001 to 0.01). In addition, external packaging of raw meats was contaminated less frequently with Campylobacter and E. coli when packaging was intact, packaging and display areas were visually clean, display temperatures were below 8°C, and hazard analysis systems were in place.

scholarly journals Occurrence of Enterobacteriaceae in Raw Meat and in Human Samples from Egyptian Retail Sellers

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Mayada Gwida ◽  
Helmut Hotzel ◽  
Lutz Geue ◽  
Herbert Tomaso
Keyword(s):  
Escherichia Coli ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Risk Of Infection ◽  
Raw Meat ◽  
Human Samples ◽  
Flight Mass Spectrometry ◽  
Health Authorities ◽  
Public Health Authorities ◽  
Meat Samples

The present study was performed to assess the presence of Enterobacteriaceae in raw meat and handlers in Egypt using cultivation and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 100 raw meat samples (chicken and beef meat, 50 each) were randomly purchased from butchers and local meat retailers located at Mansoura city, Egypt. Fifty human samples were collected from meat handlers (hand swabs and stool specimens, 25 each). 228 bacterial isolates were recovered from these samples. Unidentified isolates were characterized by partial 16S rRNA gene sequencing. Escherichia coli isolates were further typed using a DNA microarray system. Proteus spp. (60.0%) were found to be the most abundant followed by Escherichia coli (38.7%), Klebsiella spp. (17.3%), and Citrobacter spp. (13.3%). The presence of different Enterobacteriaceae in locally produced retail raw meat demonstrates the risk of infection of people through consumption of raw or undercooked meat and the risk for cross-contamination of other food products. Harmonized and concerted actions from veterinary and public health authorities are needed to reduce the risk of infection.

Prevalence and Antibiotic Resistance Profiles of Escherichia coli O157:H7 in Beef Products from Retail Outlets in Gaborone, Botswana

2005 ◽  
Vol 68 (2) ◽  
pp. 403-406 ◽  
Author(s):  
CLIFF A. MAGWIRA ◽  
BERHANU A. GASHE ◽  
ERNEST K. COLLISON
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Escherichia Coli O157 ◽  
Potassium Tellurite ◽  
Selective Enrichment ◽  
E Coli ◽  
Retail Outlets ◽  
Beef Products ◽  
Meat Samples ◽  
Minced Meat

Four hundred meat samples (134 meat cubes, 133 minced meat, 133 fresh sausages) were collected from 15 supermarkets and butcheries in Gaborone, Botswana, between the summer months of October 2002 and March 2003. Samples were assayed for Escherichia coli O157 by selective enrichment in modified E. coli broth containing novobiocin, followed by immuno-magnetic separation and plating onto sorbitol MacConkey agar supplemented with potassium tellurite. The isolates were biochemically and serologically confirmed by API 20E and O157 antisera, respectively. The prevalence rates for E. coli O157 were 5.22% in meat cube samples, 3.76% in minced meat samples, and 2.26% in fresh sausages. The isolates showed single, double, and triple antibiotic resistance. Fifty-three percent of them were resistant to cephalothin. Resistance was also recorded for sulphatriad (33%), colistin sulphate (26%), streptomycin (0.7%), and tetracycline (26%). It is recommended that the cause for antibiotic resistance be investigated using a larger number of samples from cattle, especially from ranching areas of the country.

scholarly journals Antibiotic Resistance in Diarrheagenic Escherichia coli Isolated from Broiler Chickens in Pakistan

2021 ◽  
Author(s):  
M. Amir ◽  
M. Riaz ◽  
Y.-F. Chang ◽  
A. Ismail ◽  
A. Hameed ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Broiler Chickens ◽  
Virulence Genes ◽  
Broiler Meat ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Polymerase Chain ◽  
Meat Samples ◽  
Open Markets

Background: Diarrheagenic Escherichia coli (DEC) strains are predominant cause of gastrointestinal tract illnesses. The main objective of the study was to determine antibiotic resistance in various types of DEC isolated from chicken broilers farmed in Pakistan. Methods: A total of 200 feces and 200 meat samples from broiler chickens were collected from the slaughtering shops in Southern Punjab, Pakistan. The confirmed fecal (n=150) and meat (n=150) E. coli isolates were investigated against 16 antibiotics. Fourteen virulence genes specific for Enteropathogenic (EPEC), Shiga Toxin-producing (STEC), Enteroinvasive (EIEC), Enteroaggregative (EAEC), and Enterotoxigenic (ETEC) E. coli were identified using Polymerase Chain Reaction. Results: EPEC was the most detected pathotype in both feces (76%) and meat (90%) samples, followed by STEC, EIEC, and ETEC. The highest resistance (40-90%) was observed against penicillin, oxytetracycline, and nalidixic acid in fecal isolates. More than 50% EPEC and EAEC fecal isolates, and 60% EAEC meat isolates were simultaneously resistant to 6 or more antibiotics. Conclusion: Conclusively, the broiler meat sold in open markets of Pakistan was considerably contaminated with multi-drug resistant DEC. To mitigate the issue, the gov- ernment should regulate the use of antibiotics at poultry farms and monitor slaughtering practices in slaughterer houses.

scholarly journals Characteristics of quinolone and beta-lactam antibiotic resistance in Salmonella isolated from raw meat in Ho Chi Minh City

2021 ◽  
Vol 4 (2) ◽  
pp. 127-137
Author(s):  
Anh Vu Truong Huynh ◽  
Khue Tu Nguyen Hoang ◽  
Hai Chu Van ◽  
Ha Huynh Yen ◽  
Duc Nguyen Anh ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antibiotic Sensitivity ◽  
Acid Resistance ◽  
Ho Chi Minh City ◽  
Beta Lactam ◽  
Salmonella Spp ◽  
Raw Meat ◽  
Beta Lactam Antibiotics ◽  
Lactam Antibiotic ◽  
Meat Samples

In this study, a total of 380 raw meat samples (pork, beef, and chicken) were collected randomly from traditional markets in Ho Chi Minh City (Vietnam). Salmonella strains were isolated by cultivation methods according to ISO 6579-1:2017, and subsequently confirmed by PCR method (TCVN 8342:2010). These strains were used to test antibiotic susceptibility in six kinds of antibiotics belonging to two groups of quinolones (nalidixic, ciprofloxacin, and ofloxacin), and β-lactam antibiotics (ampicillin, amoxicillin, cephalexin) together with the detection of their resistant genotypes was estimated by Kirby-Bauer method and multiplex PCR. It was noted that the proportion of Salmonella spp. contaminated samples was 42.37% (161/380). Specifically, Salmonella spp. strains found in 50.00 % (63/126), 49.62% (65/131), and 26.83% (33/123) of pork, chicken, and beef samples, respectively. It was noticed that all isolated strains were resistant to six types of antibiotics. The highest proportion was 22.98% for ampicillin (AM), followed by 10.56% for nalidixic acid (NA). The proportions of amoxicillin/acid clavulanic (AMC), ceftazidime (CAZ), ciprofloxacin (CIP), and ofloxacin (OPX) resistant strains were remarkably low (< 10%). There were 37/161 (22.98%) Salmonella strains carrying TEM genes and 5/161 (3.11%) carrying CTX genes. On the other hand, there was no strain carrying SHV genes. Four quinolone-resistant genes including gyrA, gyrB involved in nalidixic acid resistance, and parC, parE involved in ofloxacin and ciprofloxacin resistance were detected in all Salmonella strains that also carried β-lactamase encoding genes. It is important to note that blaTEM-gyrA/B-parC/E genes were simultaneously found in all tested strains. While the proportion of strains containing blaCTX-gyrA/B-parC/E genes was 40.54%, the proportion of strains carrying blaTEM/CTX-gyrA/B-parC/E genes was 5.41%. The results revealed that raw meat might be contaminated with Salmonella spp. that are highly resistant to β-lactam antibiotics and quinolones. Henceforth, it is necessary to establish monitoring and surveillance programs on Salmonella spp. contamination and their antibiotic sensitivity in Vietnam to protect consumers’ health. The study also provided direct evidence for Salmonella contamination and antibiotic resistance situation in Ho Chi Minh City.

Sign in / Sign up

Export Citation Format

Share Document