scholarly journals Antibiotic Resistance in Escherichia coli and Staphylococcus aureus from Retail Chicken Meat in Surabaya, Indonesia

2020 ◽  
Vol 3 (2) ◽  
pp. 80
Author(s):  
Annisa Aulia Maghfirani Sudarmadi ◽  
Subur Prajitno ◽  
Agung Dwi Wahyu Widodo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Growth Promotion ◽  
Infectious Agent ◽  
Sampling Technique ◽  
Chicken Meat ◽  
Meat Sample ◽  
Meat Samples ◽  
And Staphylococcus Aureus

Introduction: Antimicrobial resistance is becoming a problem in public health. Zoonotic food-borne bacteria is infectious agent that can be transferred from animal to human through the food-producing animal we consume. Nowadays, antibiotic used for human and animal is not only to cure infection but also to aim animal’s growth promotion. It is known as non therapeutic antimicrobial agent (NTA) leading to antibiotic resistance. The third generation cephalosporins, cefotaxime, and also cefoxitin are included as important antibiotic for human. This study aims to identify the presence of cefotaxime-resistant Escherichia coli and cefoxitin-resistant Staphylococcus aureus isolated from chicken meat of both traditional and modern market in Surabaya.Methods: This is descriptive post test only experimental research. We used 8 samples of chicken meat from 4 different market using purposive sampling technique. We cultured Escherichia coli and Staphylococcus aureus from the chicken meat. Sensitivity test was done using Kirby-bauer disk-diffusion method.Results: All chicken meat sample bought from traditional market in Surabaya are contaminated by cefotaxime-sensitive Escherichia coli (n=4/4) while chicken meat sample bought from modern market are not contaminated by Escherichia coli (n=0/4). All chicken meat sample bought from traditional (n=4/4) are also contaminated by cefoxitin-sensitive Staphylococcus aureus. Half of chicken meat sample bought from modern market (n=2/4) are contaminated by cefoxitin-sensitive Staphylococcus aureus, while the other half (n=2/4) are contaminated by cefoxitin-resistant Staphylococcus aureus.Conclusion: Antibiotic resistance is found and all chicken meat samples have been highly contaminated with bacteria therefore food-processing should be done correctly.

scholarly journals  Antibiotic resistance of enterococci, coagulase negative staphylococci and Staphylococcus aureus isolated from chicken meat

2013 ◽  
Vol 31 (No. 1) ◽  
pp. 14-19 ◽  
Author(s):  
N.E. Yurdakul ◽  
Z. Erginkaya ◽  
E. Ünal
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Chicken Meat ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
Enterococcus Spp ◽  
Meat Samples ◽  
And Staphylococcus Aureus

We determined the antibiotic resistance of enterococci, coagulase negative staphylococci, and Staphylococcus aureus isolated from chicken meat samples. The antibiotic resistance of the isolated strains was estimated by the Kirby-Bauer disk diffusion method (according to the NCCLS document M2-A9 suggestions). It was found that all strains of Enterococcus spp. were resistant to tetracycline, 75% of them were resistant to ciprofloxacin, and 50% of them were resistant to erythromycin, vancomycin, and chloramphenicol. Also all strains of S. aureus were resistant to tetracycline and 25% of S. aureus strains were resistant to erythromycin and chloramphenicol, whereas all strains of S. aureus were sensitive to teicoplanin and 25% of them were sensitive to vancomycin and ciprofloxacin. As for the isolate of coagulase negative staphylococci (CNS), 68.1% of them were resistant to erythromycin, 77.2% of them were resistant to tetracycline, 59% of them were resistant to vancomycin, 9% of them were resistant to teicoplanin, and 27.2% of them were resistant to both chloramphenicol and ciprofloxacin. As a result, it was found that most of the strains (all of S. aureus and Enterococcus spp., also 77.2% CNS) were resistant to tetracycline.  

scholarly journals Prevalence of Escherichia Coli, Salmonella Sp, Staphylococcus Aureus Bacteria in Chicken Meat of Traditional Market Surabaya City

2021 ◽  
Vol 8 (2) ◽  
pp. 193-204
Author(s):  
Ulfa Dwi Karisma ◽  
Nurul Wiqoyah ◽  
Suhintam Pusarawati
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Chicken Meat ◽  
Plate Count ◽  
Test Results ◽  
E Coli ◽  
Traditional Markets ◽  
Two Samples ◽  
Contaminated Food ◽  
And Staphylococcus Aureus

Foodborne disease is a disease caused by contaminated food. Chicken meat is very susceptible to contamination by bacteria. Escherichia coli, Salmonella sp, and Staphylococcus aureus are types of bacteria found in chicken meat. The purpose of this study was to see how many Escherichia coli, Salmonella sp, and Staphylococcus aureus colonies in raw chicken meat in traditional markets in Surabaya City. The research sample used was 30 samples of chicken meat taken in 5 traditional markets in Surabaya. Six chicken meat sellers were taken with 1 sample and put in the sterile tube from each market. The test was carried out using the Total Plate Count (TPC) method. The TPC test results showed that all samples were contaminated with E. coli, with the most colony being 2.2X10-7 CFU/g while the minor colony was 5X10-4 CFU/g. There are two samples contaminated with Salmonella sp, in the G market and the W market, with code D6 4.7X10-6 CFU/g and code E5 1X10-6 CFU/g. Meanwhile, S. aureus was only found in the W market with sample codes E1 & E6, and each sample had a colony of 2.7X 10-7 CFU/g and 2X10-5 CFU/g.

scholarly journals Comparative bacteriological analysis of frozen and fresh chicken meats sold around old site Bayero University Kano

2021 ◽  
Vol 14 (1) ◽  
pp. 258-262
Author(s):  
Habibu Usman Abdu ◽  
Aisha Mustapha Abubakar
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Chicken Meat ◽  
Salmonella Spp ◽  
E Coli ◽  
Meat Samples ◽  
And Staphylococcus Aureus ◽  
Klebsiella Spp ◽  
Campylobacter Spp

Foodborne pathogens are serious public healthproblems that greatly affect the cost of food production and healthcare while poultry are often associated with foodborne disease outbreaks. This study aimed at investigating the occurrence and distribution of bacterial pathogens associated with chicken meats sold around Bayero University, Kano old site. A total of twenty (20) meat samples comprised of 10 frozen and ten freshly slaughtered chicken were purchased from different selling outlets around the study area. The samples were processed using standard cultural and biochemical procedures. The results of the study showed the mean aerobic mesophilic bacterial count of 5.93 x 106 CFU/g and 3.12 x 106  CFU/g for frozen and freshly slaughtered chicken meat samples respectively. The coliform counts were 420.1 and 347.7 CFU/100g for frozen and freshly slaughtered chicken meat samples respectively. The distribution of different bacterial species revealed E. coli (26.0%), Klebsiella spp (4.0%), Salmonella spp (19.5%), Campylobacter spp. (14.6%) and Staphylococcus aureus (30%) in frozen chicken meat samples while freshly slaughtered chicken meat samples revealed E. coli (21.0%), Klebsiella spp (8.9%), Salmonella spp (30%), Campylobacter spp. (15.0%) and Staphylococcus aureus (24.0%). This showed that frozen chicken meats were more contaminated than freshly slaughtered chicken meats which might be associated with poor handling and storage conditions.

scholarly journals Antibacterial Activity of Lemongrass (Cymbopogon nardus) Ethanolic Extract

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Waras Nurcholis ◽  
Mira Takene ◽  
Ratna Puspita ◽  
Lisnawati Tumanggor ◽  
Eka Nurul Qomariyah ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibiotic Resistance ◽  
Infectious Diseases ◽  
Ethanolic Extract ◽  
Leaf Extracts ◽  
E Coli ◽  
Cymbopogon Nardus ◽  
And Staphylococcus Aureus

Infectious diseases are a disease caused by bacteria such as Escherichia coli and Staphylococcus aureus. One way to overcome the disease by using antibiotics. However, wise use can lead to antibiotic resistance, so many bacteria are resistant to antibiotics. Therefore it is necessary to search the natural compounds as antibacterials, one of them by using lemongrass (Cymbopogon nardus). This study aims to determine the effectiveness of leaf extracts, stems, and roots lemongrass as antibacterial. The method used in this research is the disc method. Bacteria used are E. coli and S. aureus. The results show extracts of roots, stems, and leaf has activity against S. aureus, while  E. coli shows no activity as antibacterial, it can be possible because of the different properties of the bacteria.   Keywords: anti-bacteria, lemongrass, Escherichia coli, Staphylococcus aureus

scholarly journals Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

2020 ◽  
Author(s):  
Thang Nam Nguyen ◽  
Diep Thi Khong ◽  
Ha Viet Le ◽  
Hoa Thi Tran ◽  
Quang Ngoc Phan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Contamination ◽  
Chicken Meat ◽  
Resistant Bacteria ◽  
Meat Sample ◽  
E Coli ◽  
Drug Resistant Bacteria ◽  
The Status ◽  
Retail Meat ◽  
Meat Samples

Abstract Objective: The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the level of resistant bacteria in food, have not yet been well elucidated.Results: In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

scholarly journals Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

2020 ◽  
Author(s):  
Thang Nam Nguyen ◽  
Diep Thi Khong ◽  
Ha Viet Le ◽  
Hoa Thi Tran ◽  
Quang Ngoc Phan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Contamination ◽  
Chicken Meat ◽  
Resistant Bacteria ◽  
Meat Sample ◽  
E Coli ◽  
Drug Resistant Bacteria ◽  
The Status ◽  
Retail Meat ◽  
Meat Samples

Abstract The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the quantitative level of resistant bacteria in food, has not yet been well elucidated. In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median quantitative levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

Incidence of Staphylococcus aureus and Escherichia coli in Ground Beef, Broilers and Processed Meats in Pretoria, South Africa

1994 ◽  
Vol 57 (4) ◽  
pp. 305-310 ◽  
Author(s):  
S. M. VORSTER ◽  
R. P. GREEBE ◽  
G. L. NORTJÉ
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Staphylococcus Aureus ◽  
Ground Beef ◽  
Processed Meat ◽  
Processed Meats ◽  
E Coli ◽  
Plate Counts ◽  
Meat Samples ◽  
And Staphylococcus Aureus

Three types of processed meats (vienna sausages, shoulder ham, and cervelat), ground beef and broilers were purchased from 17 different supermarkets in the Pretoria area (South Africa) during 1991. The 232 samples were analyzed for the presence of Escherichia coli and Staphylococcus aureus, with total aerobic plate counts (APCs) also being determined. Escherichia coli was found in 74.5% of the ground beef samples, in 79.1% of the broilers, and in 27.7% of the processed meats. Staphylococcus aureus was found in 23.4% ground beef, 39.5% broiler and 7.1% processed meat samples. The total APCs ranged from as low as log10 1 CFU/g of sample (shoulder ham) to as high as log10 12.1 CFU/g (ground beef). No identifiable relationship between the total APCs and the occurrence of E. coli and/or S. aureus was evident. This study confirms the view that E. coli and S. aureus are frequent contaminants of meat, with South Africa being no exception.

scholarly journals Antimicrobial Drug Resistance of Escherichia coli and Staphylococcus aureus Isolated from Milk and Milk Based Beverages of Dhaka City, Bangladesh

2021 ◽  
Author(s):  
Khondaker Wahid Hasan ◽  
Nourin Tarannum ◽  
Sahana Parveen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Raw Milk ◽  
Penicillin G ◽  
Resistance Pattern ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Antibiotic Resistance Pattern ◽  
And Staphylococcus Aureus

The increase of pathogens in milk is threatening for the human beings. This is an investigation on overall microbiological analysis of raw and pasteurized milk as well as the milk-based beverages and also determining the antibiotic resistance pattern of isolated Escherichia coli and Staphylococcus aureus. A total of 100 samples (raw milk, pasteurized milk, mattha, lassi and laban) were taken from various locations of the capital city of Bangladesh, Dhaka. Total Viable Count, Total Coliform Count and Yeast and Moulds Count were performed as the microbiological inspection of selected samples. E.coli and Staphylococcus aureus were identified by conducting morphological analysis, gram-staining and biochemical tests. Antibiotic resistance pattern of isolated Escherichia Coli and Staphylococcus aureus were also detected with 11 commonly used antibiotics by conducting disc-diffusion method, following the CLSI guideline. The TVC range was the highest in raw milk samples (3.8×104 – 4.1×108 cfu/ml), and the lowest in pasteurized milk samples (1.2×102 – 5.4×103 cfu/ml), while 70% raw milk and 10% pasteurized milk samples strains were above the acceptable limit of Food and Drug Administration (FDA). Thirty-six Escherichia coli and thirty-two Staphylococcus aureus were isolated from all the 100 milk and milk-based beverage samples. The isolated Escherichia coli strains were most resistant to Penicillin G (81.58%), Erythromycin (78.94%) and Ampicillin (73.68%), and isolated Staphylococcus aureus strains were most resistant to Penicillin G (90.62%), Ampicillin (81.25%) and Methicillin (71.87%). respectively. Public awareness is needed to reduce the redundant use of antibiotics.

scholarly journals Quantitative Analysis of Colistin-Resistant Escherichia coli in Retail Meat from Local Vietnamese Markets

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Thang N. Nguyen ◽  
Diep T. Khong ◽  
Ha V. Le ◽  
Hoa T. Tran ◽  
Quang N. Phan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Contamination ◽  
Chicken Meat ◽  
Resistant Bacteria ◽  
Meat Sample ◽  
E Coli ◽  
Drug Resistant Bacteria ◽  
The Status ◽  
Retail Meat ◽  
Meat Samples

The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the quantitative level of resistant bacteria in food, has not yet been well elucidated. In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene, mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median quantitative levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 10 4 and 4.2 × 10 3  CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli was a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

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