Identification of chemical compounds in essential oils from stems, leaves and flowers of Mentha longifolia Var. kermanensis by GC/MS

Abstract Essential oils (EOs) from aerial parts from Mentha longifolia (Saudi cultivar) and Citrus reticulata grown in Egypt, were analyzed by GC/MS to identify their chemical compounds. The EOs were tested for their antifungal activity against four common fungi Aspergillus flavus, A. niger, A. fumigatus, and Fusarium culmorum. The most abundant compounds identified in the EO from M. longifolia were menthone, and eucalyptol with percentages of 48.00%, and 21.66%, respectively. The main chemical compounds from C. reticulata EO were β-caryophyllene, β-caryophyllene oxide, and β-elemene with values of 15.57%, 7.04%, and 6.39%, respectively. At the concentrations of 500 and 250 µL/mL, the EOs from M. longifolia and C. reticulata showed potent antifungal activity against A. flavus and A. fumigatus with 100% fungal mycelial inhibition growth (FMIG). FMIG with values of 98% and 95% were observed against A. fumigatus by C. reticulata and M. longifolia EOs, respectively, at 125 µL/mL. At 500 and 250 µL/mL levels of concentration from the EO of M. longifolia, potent antifungal activity found against A. niger with 100% FMIG. F. culmorum completely inhibited (100% FMIG) as EOs from M. longifolia and C. reticulata applied at 500 µL/mL. Pinus halepensis wood treated with M. longifolia at 125 µL/mL showed inhibition zone values of 7.33 mm, 21.33 mm A. flavus and Aspergillus niger, respectively. The results suggested the potential values of the EOs as potent antifungal activity against some common molds that grows over wood packaging during transportation of vegetables and fruits or can cause food spoilage.

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Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase

Genes ◽

10.3390/genes12040518 ◽

2021 ◽

Vol 12 (4) ◽

pp. 518

Author(s):

Zequn Chen ◽

Xiwu Qi ◽

Xu Yu ◽

Ying Zheng ◽

Zhiqi Liu ◽

...

Keyword(s):

Catalytic Activity ◽

Essential Oils ◽

Gene Family ◽

Genome Sequence ◽

Sequence Assembly ◽

Terpene Synthase ◽

Genome Sequence Assembly ◽

Mentha Longifolia ◽

Specific Expansion ◽

Species Specific

Terpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. Mentha plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However, there is a lack of systematic identification and study of TPS in Mentha plants. In this work, we genome-widely identified and analyzed the TPS gene family in Mentha longifolia, a model plant for functional genomic research in the genus Mentha. A total of 63 TPS genes were identified in the M. longifolia genome sequence assembly, which could be divided into six subfamilies. The TPS-b subfamily had the largest number of genes, which might be related to the abundant monoterpenoids in Mentha plants. The TPS-e subfamily had 18 members and showed a significant species-specific expansion compared with other sequenced Lamiaceae plant species. The 63 TPS genes could be mapped to nine scaffolds of the M. longifolia genome sequence assembly and the distribution of these genes is uneven. Tandem duplicates and fragment duplicates contributed greatly to the increase in the number of TPS genes in M. longifolia. The conserved motifs (RR(X)8W, NSE/DTE, RXR, and DDXXD) were analyzed in M. longifolia TPSs, and significant differentiation was found between different subfamilies. Adaptive evolution analysis showed that M. longifolia TPSs were subjected to purifying selection after the species-specific expansion, and some amino acid residues under positive selection were identified. Furthermore, we also cloned and analyzed the catalytic activity of a single terpene synthase, MlongTPS29, which belongs to the TPS-b subfamily. MlongTPS29 could encode a limonene synthase and catalyze the biosynthesis of limonene, an important precursor of essential oils from the genus Mentha. This study provides useful information for the biosynthesis of terpenoids in the genus Mentha.

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Essential Oils from Indigenous Iranian Plants: A Natural Weapon vs. Multidrug-Resistant Escherichia coli

Microorganisms ◽

10.3390/microorganisms10010109 ◽

2022 ◽

Vol 10 (1) ◽

pp. 109

Author(s):

Mohammadreza Pajohi Alamoti ◽

Behnaz Bazargani-Gilani ◽

Razzagh Mahmoudi ◽

Anna Reale ◽

Babak Pakbin ◽

...

Keyword(s):

Escherichia Coli ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Properties ◽

Multidrug Resistant ◽

Zataria Multiflora ◽

Cuminum Cyminum ◽

Plant Essential Oils ◽

E Coli ◽

Mentha Longifolia

Aim of this study was to investigate the antimicrobial properties of herbal plant essential oils (EOs) from selected Iranian plant species such as Ferulago angulata, Zataria multiflora, Cuminum cyminum, and Mentha longifolia against antibiotic-resistant Escherichia coli (E. coli) strains. For this purpose, the Escherichia coli strains, isolated from raw cow’s milk and local dairy products (yogurt, cream, whey, cheese, and confectionery products) collected from different areas of Hamedan province, Iran, were investigated for their resistance to antibiotics (i.e., streptomycin, tetracycline, gentamicin, chloramphenicol, ciprofloxacin, and cefixime). Thus, the E. coli strains were tested for their susceptibility to the above-mentioned essential oils. Regarding antibiotics, the E. coli strains were highly sensitive to ciprofloxacin. In relation to essential oils, the most effective antibacterial activity was observed with Zataria multiflora; also, the bacteria were semi-sensitive to Cuminum cyminum and Mentha longifolia essential oils. All strains were resistant to Ferulago angulata essential oil. According to the results, the essential oil of Zataria multiflora can be considered as a practical and alternative antibacterial strategy to inhibit the growth of multidrug-resistant E. coli of dairy origin.

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CHEMICAL COMPOSITION, ADULTICIDAL AND REPELLENT ACTIVITY OF ESSENTIAL OILS FROM Mentha longifolia L. AND Lavandula dentata L. AGAINST Culex pipiens L.

Journal of Plant Protection and Pathology ◽

10.21608/jppp.2014.87995 ◽

2014 ◽

Vol 5 (7) ◽

pp. 817-826

Author(s):

Ali Al-Sarar

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Culex Pipiens ◽

Repellent Activity ◽

Mentha Longifolia

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Investigating the Effects of Major Ecological Factors on Essential Oils and Existing Chemical Compounds of Nepeta menthoides in Habitat Conditions

Environment Conservation Journal ◽

10.36953/ecj.2015.se1654 ◽

2015 ◽

Vol 16 (SE) ◽

pp. 457-459

Author(s):

Mozhgan Omidi ◽

Ali Akbar Imani

Keyword(s):

Essential Oils ◽

Standard Condition ◽

Ecological Factors ◽

Chemical Compounds ◽

Hot Water ◽

Research Station ◽

Natural Habitats ◽

Water Steam ◽

North West ◽

Specific Species

A native Iranian plant, Nepeta menthoides, located and distributed in the north west of Iran, is a gramineous, perennial plant species which belong s to mint family. Highlands of Sabalan Mountain, in Ardebil province, Shabil and Gotur Suyi regions are habitats for this specific species. This study enjoys samples collected from selective habitats in the stage of 50% flowering; moreover, the collected samples were dried in an appropriate and standard condition. Abooreyhan laboratory of Jungle and Pasture research institute was the place in which the above mentioned samples have undergone the process of essential oils, using the water steam method of distillation. The extracted components were gauged and identified by means of gas chromatography system connected to mass spectrograph. The results of the study distinctly indicated that in the habitat condition, the species grows in the highlands of Sabalan Mountain over the attitude of 3200 to 4000 meters, this specific species is highly visible in series and sandy clay hills, it is demonstrated that the density of the species has been increased as the height was increasing. Essential oil in the investigated area was ranging from 1.6% to 2.40%, admittedly, the least amount was in Gotur Suyi, while, the highest amount belonged to Shabil. Nineteen chemical compounds were identified in samples collected from natural habitats, amongst all, the highest proportion belongs to 8,10-Cineole and Citronellol acetate.8,10- Cineole was ranging from 33 to 34 percent, the least amount was identified in Gotur Suyi region (before hot water spa) and the highest amount was observed between Gotur Suyi and Shabil. As mentioned earlier, Citronellol acetate was the second chemical compound, which was ranging from 16% to 31% and the least and the highest amount were known in Gotur suyi and Shabil, respectively. Samian medicinal plants research station possesses the highest level with the value of 57%.

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Synergistic effects of deuterium depleted water and Mentha longifolia L. essential oils on sepsis-induced liver injuries through regulation of cyclooxygenase-2

Pharmaceutical Biology ◽

10.1080/13880209.2018.1563622 ◽

2019 ◽

Vol 57 (1) ◽

pp. 125-132 ◽

Cited By ~ 7

Author(s):

Azadeh Rasooli ◽

Faezeh Fatemi ◽

Reza Hajihosseini ◽

Atoosa Vaziri ◽

Kambiz Akbarzadeh ◽

...

Keyword(s):

Essential Oils ◽

Synergistic Effects ◽

Cyclooxygenase 2 ◽

Liver Injuries ◽

Mentha Longifolia ◽

Deuterium Depleted Water

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Chemical Composition and Antimicrobial and Antioxidant Activities of Mentha (longifolia L. and viridis) Essential Oils

Journal of Food Science ◽

10.1111/j.1750-3841.2009.01272.x ◽

2009 ◽

Vol 74 (7) ◽

pp. M358-M363 ◽

Cited By ~ 91

Author(s):

Mounira Mkaddem ◽

Jalloul Bouajila ◽

Monia Ennajar ◽

Ahmed Lebrihi ◽

Florence Mathieu ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Antioxidant Activities ◽

Mentha Longifolia

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Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia

Food Chemistry ◽

10.1016/j.foodchem.2006.10.061 ◽

2007 ◽

Vol 103 (4) ◽

pp. 1449-1456 ◽

Cited By ~ 267

Author(s):

M. Gulluce ◽

F. Sahin ◽

M. Sokmen ◽

H. Ozer ◽

D. Daferera ◽

...

Keyword(s):

Essential Oils ◽

Methanol Extract ◽

Antioxidant Properties ◽

Mentha Longifolia

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Essential Oils from Two Endemic Species of Apiaceae from Iran

Zeitschrift für Naturforschung C ◽

10.1515/znc-2003-7-802 ◽

2003 ◽

Vol 58 (7-8) ◽

pp. 459-463 ◽

Cited By ~ 10

Author(s):

Narguss Yassa ◽

Hossein Akhani ◽

Majid Aqaahmadi ◽

Mojtaba Salimian

Keyword(s):

Essential Oils ◽

Endemic Species ◽

Chemical Compounds ◽

Oxygenated Monoterpenes ◽

Monoterpene Hydrocarbons ◽

Sesquiterpene Hydrocarbons

Abstract The composition of essential oils of Leutea glaucopruinosa (Rech.f.) Akhani & Salimian comb, nov., and Zeravschania (Boiss. & Hausskn.) Salimian & Akhani comb. nov. were analysed by GC-MS. 49 compounds are identified in the former and 33 compounds in the latter, comprising a total of 76 compounds in both species. Both species were originally described under Peucedanum, which are transferred in this paper into Leutea and Zeravschania, respectively. The chemical compounds of the essential oils show that there are only seven common compounds between two species. The major compounds of L. glaucopruinosa are mostly monoterpene hydrocarbons and oxygenated monoterpenes, in which α-pinene (31.5%), sabinene (9.7%), β-pinene (9.2%), exo-fenchyl acetate (4.5%) are dominant. In Z. pastinacifolia sesquiterpene hydrocarbons and phenylpropanoids dominate with β-bisabolene (37.3%), 3,1-butyl-1.2-dimethoxy benzene (14.9%), 10,11-dimethylbicyclo[6.3.0]undec-(8)-en-9-one (12.9%), 4-t-butyl-1,2-dimethoxy benzene (6.8%), (E)-asarone (5.1%) and elemicine (4.1%) as major compounds.

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CHEMICAL COMPOUNDS AND ANTIOXIDANT CONTENTS OF CLOVES LEAVES ESSENTIAL OIL

AGROLAND The Agricultural Sciences Journal ◽

10.22487/agroland.v7i1.459 ◽

2020 ◽

Vol 7 (1) ◽

pp. 37-44

Author(s):

Muhammad Tahir ◽

Siti Chuzaemi ◽

Eko Widodo ◽

Hafsah Hafsah

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Chemical Compounds ◽

Antioxidant Content ◽

Ic50 Values ◽

Anti Fungal ◽

Central Sulawesi

Essential oils from the distillation of clove leaves could act as anti-bacterial and anti-fungal agentsforeither food or feed. The purpose of this study was to identify chemical compounds and antioxidant content of clove leaves essential oils. The material used was clove leaves essential oil obtained by soxlet extraction. Clove leaves were taken from trees of over 10 years old in Palu, Central Sulawesi. Chemical compounds of the sample were analyzed by GC-MS method. Each peak that appeared on the chromatogram was identified by comparing to the standard. While the antioxidant analysis was carried out by using the DPPH (IC50) of spectrophotometry method. The results showed that the total essential oils content of clove leaves was 3,957% and there were 6 chemical compounds identified, i.e. caryophyllen (0,96%); phenol, 2-methoxy (guaiacol) (4,16%); phenol,2-methoxy-4-methyl (9.79%); phenol, 4-ethyl-2-methoxy (1.5%); phenol 2-methoxy-4-propyl (3.87%); phenol, 2-methoxy-4-(2-prophenyl) andEugenol (79.72%). While the results of antioxidant identification based on DPPH and IC50 values before purification were 0.597 and 74.358, and the respective values after purification were 0.597 and 42.255. In conclusion, the antioxidant content of clove leaves essential oils after purification is higher than before purification and it contains 3.957% essential oil.

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