scholarly journals Mentha longifolia and Citrus reticulata Essential Oils: Potential wood-biofungicide and MNDO Quantum Chemical Studies

2020 ◽  
Author(s):  
Hayssam M Ali ◽  
Wael AA Abo Elgat ◽  
Mervat EL-Hefny ◽  
Mohamed ZM Salem ◽  
Ayman S Taha ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Pinus Halepensis ◽  
Fusarium Culmorum ◽  
Chemical Compounds ◽  
Inhibition Zone ◽  
Citrus Reticulata ◽  
Mentha Longifolia ◽  
Chemical Studies ◽  
Vegetables And Fruits

Abstract Essential oils (EOs) from aerial parts from Mentha longifolia (Saudi cultivar) and Citrus reticulata grown in Egypt, were analyzed by GC/MS to identify their chemical compounds. The EOs were tested for their antifungal activity against four common fungi Aspergillus flavus, A. niger, A. fumigatus, and Fusarium culmorum. The most abundant compounds identified in the EO from M. longifolia were menthone, and eucalyptol with percentages of 48.00%, and 21.66%, respectively. The main chemical compounds from C. reticulata EO were β-caryophyllene, β-caryophyllene oxide, and β-elemene with values of 15.57%, 7.04%, and 6.39%, respectively. At the concentrations of 500 and 250 µL/mL, the EOs from M. longifolia and C. reticulata showed potent antifungal activity against A. flavus and A. fumigatus with 100% fungal mycelial inhibition growth (FMIG). FMIG with values of 98% and 95% were observed against A. fumigatus by C. reticulata and M. longifolia EOs, respectively, at 125 µL/mL. At 500 and 250 µL/mL levels of concentration from the EO of M. longifolia, potent antifungal activity found against A. niger with 100% FMIG. F. culmorum completely inhibited (100% FMIG) as EOs from M. longifolia and C. reticulata applied at 500 µL/mL. Pinus halepensis wood treated with M. longifolia at 125 µL/mL showed inhibition zone values of 7.33 mm, 21.33 mm A. flavus and Aspergillus niger, respectively. The results suggested the potential values of the EOs as potent antifungal activity against some common molds that grows over wood packaging during transportation of vegetables and fruits or can cause food spoilage.

scholarly journals New Approach for Using of Mentha longifolia L. and Citrus reticulata L. Essential Oils as Wood-Biofungicides: GC-MS, SEM, and MNDO Quantum Chemical Studies

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1361
Author(s):  
Hayssam M. Ali ◽  
Wael A. A. Abo Elgat ◽  
Mervat EL-Hefny ◽  
Mohamed Z. M. Salem ◽  
Ayman S. Taha ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Fusarium Culmorum ◽  
Inhibition Zone ◽  
Gas Chromatography Mass Spectrometry ◽  
Citrus Reticulata ◽  
Pinus Roxburghii ◽  
Mentha Longifolia ◽  
Chemical Studies ◽  
Vapor Method

Background: Fungi growing on wood cause deterioration of stored food materials or discoloration of the wood itself, and the search for new and safe bioagents is recently needed. Methods: Essential oils (EOs) from aerial parts from Mentha longifolia L. and Citrus reticulata L., analyzed by gas chromatography-mass spectrometry (GC-MS), were tested for their antifungal activity by the vapor method against four common fungi, Aspergillus flavus, A. niger, A. fumigatus, and Fusarium culmorum, and confirmed by SEM examination as the oils applied on wood samples. Results: The most abundant compounds identified in the EO from M. longifolia were menthone and eucalyptol; in C. reticulata EO, they were β-caryophyllene, β-caryophyllene oxide, and β-elemene. EOs from M. longifolia and C. reticulata, at 500 and 250 µL/mL, showed potent antifungal activity against A. flavus and A. fumigatus, with 100% fungal mycelial inhibition growth (FMIG). C. reticulata and M. longifolia EOs, at 125 µL/mL, observed FMIG values of 98% and 95%, respectively, against A. fumigatus. M. longifolia EO, at 500 and 250 µL/mL, showed potent activity against A. niger, with 100% FMIG. F. culmorum completely inhibited (100% FMIG) EOs from M. longifolia and C. reticulata applied at 500 µL/mL. Pinus roxburghii Sarg. Wood, treated with M. longifolia at 125 µL/mL, showed inhibition zone values of 7.33 and 21.33 mm against A. flavus and A. niger, respectively. Conclusions: Both oils possessed good wood-biofungicide activity with the vapor method, as clearly shown by the SEM examination. These activities suggest their possible use as natural wood preservatives.

scholarly journals Antifungal activity of Mentha piperita and Carum carvi essential oils

2017 ◽  
pp. 201-207 ◽  
Author(s):  
Dragana Plavsic ◽  
Gordana Dimic ◽  
Djordje Psodorov ◽  
Dragan Psodorov ◽  
Ljubisa Saric ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Biologically Active ◽  
Mentha Piperita ◽  
Diffusion Method ◽  
Aromatic Plants ◽  
Disc Diffusion Method ◽  
Penicillium Aurantiogriseum

Aromatic plants are one of the most important sources of biologically active secondary metabolites, which possess various antimicrobial characteristics. The aim of this work was to examine the effect of antifungal activities of mint and caraway essential oils against the selected fungi. Eight species of molds were selected for antifungal testing: Alternaria alternata, Aspegillus flavus, A. niger, A. versicolor, Eurotium herbariorum, Penicillium aurantiogriseum, P. chrysogenum and P. expansum. Testing of essential oils antifungal activity against the selected species was conducted using the disc diffusion method by adding mint and caraway essential oils (0.5, 1, 5, and 10 ?l per disc). Antifungal activity of essential oils was expressed by the diameter of inhibition zone (mm). The most powerful effect of mint essential oil was recorded against E. herbariorum, as its growth was completely inhibited by the quantity of 5 ?l. The weakest inhibitory effect was observed against P. chrysogenum (inhibition zone 13.67 mm) by the quantity of 10 ?l. The most powerful antifungal activity of caraway was observed against E. herbariorum as growth was completely inhibited by the quantity of 10 ?l. The weakest inhibitory effect was observed against A. niger (inhibition zone 28 mm) by the quantity of 10 ?l.

scholarly journals Antifungal activity of Ocotea odorifera (Vell.) Rowher, Ocotea puberula (Rich.) Nees and Cinnamodendron dinisii Schwanke essential oils

2019 ◽  
Author(s):  
P. Mezzomo ◽  
T.L. Sausen ◽  
N. Paroul ◽  
S.S. Roman ◽  
A.A.P. Mielniczki ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Chemical Compounds ◽  
Inhibitory Effect ◽  
Pathogenic Microorganisms ◽  
Biological Interest ◽  
Penicillium Crustosum

AbstractBiocompounds are promising tools with the potential to control pathogenic microorganisms. The medicinal plant species Ocotea odorifera, Ocotea puberula and Cinnamodendron dinisii, distributed along Brazilian biomes, are sources of chemical compounds of biological interest. This study aimed to evaluate the antifungal activity of the essential oils of O. odorifera, O. puberula and C. dinisii essential oils upon the mycotoxin producers Alternaria alternata, Aspergillus flavus and Penicillium crustosum. The essential oils where characterized by gas chromatography coupled to mass spectrometer (CG-MS). The majority compounds identified were: safrol (39.23%) and camphor (31.54%) in O. odorifera, Beta-caryophyllene (25.01%) and spathulenol (17.74%) in O. puberula, and bicyclogermacrene (23.19%) and spathulenol (20.21%) in C. dinisii. The Minimal Inhibitory Concentration (MIC) of antifungal activity considered diameters higher than 10 mm after 72 h of incubation at 30 ºC. A. alternata presented higher resistance to O. odorifera and C. dinisii oils. The inhibitory effect of O. odorifera on A. flavus showed stabilization at oils concentrations between 50% and 80%, increasing at 90% and 100% (pure oil) treatments. We observed that the essential oils of O. odorifera and C. dinisii have potential in the control of the analyzed fungi species. The essential oil of O. odorifera presented a better activity in all the assays, which can be related to the presence of safrole and phenylpropenes, compounds with known antifungal activity.

scholarly journals Eucalyptus camaldulensis, Citrus aurantium, and Citrus sinensis Essential Oils as Antifungal Activity against Aspergillus flavus, Aspergillus niger, Aspergillus terreus, and Fusarium culmorum

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 1003
Author(s):  
Wael A.A. Abo Elgat ◽  
Ahmed M. Kordy ◽  
Martin Böhm ◽  
Robert Černý ◽  
Ahmed Abdel-Megeed ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Aspergillus Flavus ◽  
Fusarium Culmorum ◽  
Eucalyptus Camaldulensis ◽  
Cost Effective ◽  
Solid Wood ◽  
Chemical Compositions ◽  
Citrus Aurantium ◽  
Aerial Parts

Several molds are able to colonize wood and many building products or solid wood causing losses for their valuable uses. Essential oils (EOs) from aromatic plants can be used as an ecofriendly biofungicide against the growth of several molds. EOs from Eucalyptus camaldulensis, Citrus aurantium, and C. sinensis have a broad-spectrum antimicrobial activity. EOs from of E. camaldulensis air-dried aerial parts, C. aurantium leaf and C. sinensis peel, and their combinations (1:1 v/v) were evaluated for their antifungal activity against the growth of four common mold fungi (Aspergillus flavus, A. niger, A. terreus, and Fusarium culmorum). The chemical compositions of the EOs were analyzed with GC/MS. The main compounds in EO from E. camaldulensis were spathulenol (20.84%), eucalyptol (12.01%), and sabinene (9.73%); in C. aurantium were linalyl acetate (42.29%), and linalool (29.76%); and in C. sinensis were D-limonene (73.4%) and γ-terpinene (22.6%). At 50 µL/mL, C. sinensis EO showed the highest fungal mycilial growth inhibition (FMGI) percentage (86.66%) against A. flavus. C. sinensis, E. camaldulensis, and E. camaldulensis/C. sinensis showed FMGI values of 96%, 91.66%, and 75.66% respectively, against A. niger. EOs from C. aurantium and C. sinensis showed potent activity against A. terreus (100% FMGI), while C. aurantium/E. camaldulensis and E. camaldulensis/C. sinensis showed FMGI values of 74.33% and 70.66%, respectively. Potent activity against F. culmorum with 100% was observed as the application of E. camaldulensis and C. sinensis EOs at 50 µL/mL, while E. camaldulensis/C. sinensis (50 µL/mL) showed FMGI value of 65.66%. The results suggest using the EOs and their combinations from E.camaldulensis, C. aurantium, and C. sinensis as a biofungicide against molds. The potent properties of EOs offer the possibility of using them as eco-friendly, safe, and cost-effective antimicrobials for molds that could cause discoloration of the wood packaging or food spoilage.

scholarly journals Comparison of In Vitro Testing Antifungal Activity between Rosemary Essentials Oil and Fluconazol on Candida species Isolate from HIV/AIDS Patients with Candidiasis Oral

2020 ◽  
Vol 32 (3) ◽  
pp. 182
Author(s):  
Putri Intan Primasari ◽  
Dwi Murtiastutik ◽  
Pepy Dwi Endraswari ◽  
Cita Rosita Sigit Prakoeswa ◽  
Evy Ervianti
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Aids Patients ◽  
Candida Sp ◽  
Significant Difference ◽  
Rosemary Essential Oil ◽  
Hiv Aids

Background: The most common opportunistic infection obtained in patients with Human Immunodeficiency Virus (HIV)/Acquired Immune Deficiency Syndrome (AIDS) is Oral Candidiasis (OC). Currently widely reported about increased fluconazole resistance, as a therapy that is often used in patients with HIV/AIDS with OC. For this reason, effective antifungal agents are needed as alternative therapies. Several studies have reported some antifungal activity from rosemary essential oils. Purpose: The aim of this study is to evaluate the antifungal activity between rosemary essential oils and fluconazole against the isolates used by Candida sp. taken from HIV / AIDS patients with OC. Methods: This research was an experimental laboratory study with a post-test only design of 40 Candida sp. isolates from HIV / AIDS patients with OC in the Outpatient Unit and Inpatient Installation of the Infectious Disease Intermediate Care Unit (UPIPI) Dr. Soetomo General Academic Teaching Hospital, Surabaya. Examination of antifungal activity using conventional test methods with disk diffusion. Result: Disk diffusion test result revealed average inhibition zone of rosemary essential oil in Candida albicans 1,75±3,64 mm and Candida non-albicans 1,5±3,08 mm, while the average inhibition zone of fluconazole in Candida albicans 17,9±8,62 mm and Candida non-albicans 4,85±7,9 mm. There was significant difference (p <0.001) between the inhibition zone formed in Candida albicans by fluconazole and rosemary essential oil, but no significant difference of inhibition zone formed in Candida non-albicans. Conclusion: Antifungal activity fluconazole has greater than Rosemary essential oils in Candida albicans.

scholarly journals Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

2019 ◽  
Vol 201 (8) ◽  
pp. 1085-1097 ◽  
Author(s):  
Adam Perczak ◽  
Daniela Gwiazdowska ◽  
Katarzyna Marchwińska ◽  
Krzysztof Juś ◽  
Romuald Gwiazdowski ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Secondary Metabolites ◽  
Essential Oils ◽  
Fusarium Culmorum ◽  
Wheat Seeds

Antifungal Activity of Essential Oils from Two Varieties of Sweet Basil (Ocimum basilicum L.)

2011 ◽  
Vol 74 (1) ◽  
pp. 97-106 ◽  
Author(s):  
Paulina Jakowienko ◽  
Barbara Wójcik-Stopczyńska ◽  
Dorota Jadczak
Keyword(s):  
Antifungal Activity ◽  
Growth Inhibition ◽  
Essential Oils ◽  
Fungal Growth ◽  
Inhibition Zone ◽  
Ocimum Basilicum ◽  
Chemical Compositions ◽  
Mycelium Growth ◽  
Sweet Basil ◽  
Inhibition Zones

Antifungal Activity of Essential Oils from Two Varieties of Sweet Basil (Ocimum basilicumL.)The aim of this work was an evaluation of the antifungal activity of essential oils from two cultivars of sweet basil (Ocimum basilicumL.): ‘Wala’ and ‘Fine Verde’. The essential oils from dried, ground herbs were extracted with the hydro-distillation method using Deryng apparatus. The composition of the oils was analyzed by gas chromatography. The antifungal activity of the oils was tested against the following strains:Alternariasp.,Aspergillus flavus, Botrytis cinerea, Cladosporium herbarum, Eurotium amstelodamiandEurotium chevalieri. The disc diffusion agar method was applied. Each oil was used in two doses, 10 and 5 μl/disc. A disc (6 mm dia.), soaked with oil, was placed on agar plates, which had been previously inoculated with spores of the different strains. The diameters of fungal growth inhibition zones were measured after 72h of incubation at 25°C and expressed in millimeters. The obtained results showed that the diameter of the mycelium growth inhibition zone significantly depended on the source of oil, the dose of oil, and the species of fungi. The essential oil from the ‘Wala’ variety was more effective against the tested fungi than the oil from ‘Fine Verde’ and the inhibition zones were greater when the dose of the oils was higher.E. chevalieriwas the most sensitive to both of the oils andA. flavuswas the most resistant. The differences in the antifungal activity of the tested oils were probably the result of their different chemical compositions. In comparison with the ‘Fine Verde’ oil, the oil from ‘Wala’ had more than twice the amount of linalool, more 1,8-cineole, and contained geraniol and methyl chavicol. In the conditions of the experiment both oils showed greater antifungal activity than cycloheximide (actidion) and methyl thiophanate (Topsin M 500 SC) used as controls.

scholarly journals Antifungal properties of Foeniculum vulgare, Carum carvi and Eucalyptus sp. essential oils against Candida albicans strains

2013 ◽  
pp. 195-202 ◽  
Author(s):  
Jelica Skrobonja ◽  
Dafina Delic ◽  
Maja Karaman ◽  
Milan Matavulj ◽  
Mirjana Bogavac
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Foeniculum Vulgare ◽  
Minimum Fungicidal Concentration ◽  
Carum Carvi ◽  
Microdilution Method

Aromatic plants are among the most important sources of biologically active secondary metabolites, with high antimicrobal potential. This study was carried out to examine in vitro antifungal activity of Foeniculum vulgare (Apiaceae), Carum carvi (Apiaceae) and Eucalyptus sp.(Myrtaceae) essential oils against three Candida albicans strains of different origin (laboratory-CAL, human pulmonary-CAH and ATCC10231-CAR). The essential oils were screened on C. albicans using disc and well-diffusion and microdilution method, and compared to Nystatine and Fluconazole as standard anti-mycotics. The activity of tested oils was expressed by inhibition zone diameter (mm), minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) (mg/ml). The results indicated that studied essential oils show antifungal activity against all three isolates of C. albicans. It was observed that each oil exhibits different degree of antifungal activity depending on the oil concentration applied as well as on analyzed strain of C. albicans. Carum carvi demonstrated the strongest antifungal effect to all tested strains, showing the lowest MIC values (0.03mg/ml for CAL, 0.06mg/ml for CAH, and 0.11mg/ml for CAR, respectively). Eucalyptus sp. exhibited the lowest antifungal activity, with MIC values ranging from 0.11 mg/ml for CAL to 0.45 mg/ml for both CAH and CAR.

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