Persimmon Leaf Extract Inhibits the ATM Activity during DNA Damage Response Induced by Doxorubicin in A549 Lung Adenocarcinoma Cells

2011 ◽  
Vol 75 (4) ◽  
pp. 650-655 ◽  
Author(s):  
Kayoko KAWAKAMI ◽  
Hiroshi NISHIDA ◽  
Naoto TATEWAKI ◽  
Yuki NAKAJIMA ◽  
Tetsuya KONISHI ◽  
...  
Keyword(s):  
Dna Damage ◽  
Lung Adenocarcinoma ◽  
Dna Damage Response ◽  
Leaf Extract ◽  
Adenocarcinoma Cells ◽  
Damage Response ◽  
Lung Adenocarcinoma Cells ◽  
Persimmon Leaf Extract ◽  
A549 Lung ◽  
Persimmon Leaf

Abstract 3875: Persimmon leaf extract inhibit gamma ray and heavy ion induced DNA damage checkpoint activity in A549 lung adenocarcinoma cells

2010 ◽  
Author(s):  
Kayoko Nakayama-Kawakami ◽  
Hiroshi Nishida ◽  
Naoto Tatewaki ◽  
Yuki Nakajima ◽  
Testuya Konishi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Leaf Extract ◽  
Gamma Ray ◽  
Heavy Ion ◽  
Dna Damage Checkpoint ◽  
Adenocarcinoma Cells ◽  
Lung Adenocarcinoma Cells ◽  
Persimmon Leaf Extract ◽  
A549 Lung ◽  
Persimmon Leaf

scholarly journals DNA damage response induced by exposure of human lung adenocarcinoma cells to smoke from tobacco- and nicotine-free cigarettes

Cell Cycle ◽  
2010 ◽  
Vol 9 (11) ◽  
pp. 2170-2176 ◽  
Author(s):  
Ellen D. Jorgensen ◽  
Hong Zhao ◽  
Frank Traganos ◽  
Anthony P. Albino ◽  
Zbigniew Darzynkiewicz
Keyword(s):  
Dna Damage ◽  
Lung Adenocarcinoma ◽  
Dna Damage Response ◽  
Human Lung ◽  
Human Lung Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Human Lung Adenocarcinoma Cells ◽  
Damage Response ◽  
Lung Adenocarcinoma Cells

Baicalein increases cisplatin sensitivity of A549 lung adenocarcinoma cells via PI3K/Akt/NF-κB pathway

2017 ◽  
Vol 90 ◽  
pp. 677-685 ◽  
Author(s):  
Meiling Yu ◽  
Benquan Qi ◽  
Wu Xiaoxiang ◽  
Jian Xu ◽  
Xiaolin Liu
Keyword(s):  
Lung Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Cisplatin Sensitivity ◽  
Lung Adenocarcinoma Cells ◽  
A549 Lung

Knockdown of integrin β4-induced autophagic cell death associated with P53 in A549 lung adenocarcinoma cells

FEBS Journal ◽  
2008 ◽  
Vol 275 (22) ◽  
pp. 5725-5732 ◽  
Author(s):  
Qiuxia He ◽  
Bin Huang ◽  
Jing Zhao ◽  
Yun Zhang ◽  
Shangli Zhang ◽  
...  
Keyword(s):  
Cell Death ◽  
Lung Adenocarcinoma ◽  
Autophagic Cell Death ◽  
Adenocarcinoma Cells ◽  
Integrin Β4 ◽  
Lung Adenocarcinoma Cells ◽  
A549 Lung

scholarly journals Promoter activation and following induction of the p21/WAF1 gene by flavone is involved in G1 phase arrest in A549 lung adenocarcinoma cells

FEBS Letters ◽  
1998 ◽  
Vol 437 (1-2) ◽  
pp. 61-64 ◽  
Author(s):  
Fulu Bai ◽  
Takayoshi Matsui ◽  
Naoko Ohtani-Fujita ◽  
Yoshizumi Matsukawa ◽  
Yi Ding ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
G1 Phase ◽  
Promoter Activation ◽  
Phase Arrest ◽  
Adenocarcinoma Cells ◽  
G1 Phase Arrest ◽  
Lung Adenocarcinoma Cells ◽  
P21 Waf1 ◽  
A549 Lung

scholarly journals ITRAQ-Based Proteomics Analysis of Triptolide On Human A549 Lung Adenocarcinoma Cells

2018 ◽  
Vol 45 (3) ◽  
pp. 917-934 ◽  
Author(s):  
Fangqiong Li ◽  
Dongxiao Zhao ◽  
Suwen Yang ◽  
Juan Wang ◽  
Qin Liu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lung Adenocarcinoma ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Cell Apoptosis ◽  
A549 Cells ◽  
Adenocarcinoma Cells ◽  
Lung Adenocarcinoma Cells ◽  
A549 Lung

Background/Aims: Triptolide (TP) is a diterpenoid triepoxide extracted from the traditional Chinese medical herb Tripterygium wilfordii that exerts prominent broad-spectrum anticancer activity to repress proliferation and induce cancer cell apoptosis through various molecular pathways. We previously observed that TP inhibits the progression of A549 cells and pancreatic cancer cells (PNCA-1) in vitro. However, the complex molecular mechanism underlying the anticancer activity of TP is not well understood. Methods: To explore the molecular mechanisms by which TP induces lung cancer cell apoptosis, we investigated changes in the protein profile of A549 cells treated with TP using a proteomics approach (iTRAQ [isobaric tags for relative and absolute quantitation] combined with NanoLC-MS/MS [nano liquid chromatography-mass spectrometry]). Changes in the profiles of the expressed proteins were analyzed using the bioinformatics tools OmicsBean and the Kyoto Encyclopedia of Genes and Genomes (KEGG) and were verified using western blotting. Apoptosis and cell cycle effects were analyzed using flow cytometry. Results: TP induced apoptosis in A549 cells and blocked A549 cells at the G2/M phase. Using iTRAQ technology, we observed 312 differentially expressed proteins associated in networks and implicated in different KEGG pathways. Gene Ontology (GO) analysis showed the overviews of dysregulated proteins in the biological process (BP), cell component (CC), and molecular function (MF) categories. Moreover, some candidate proteins involved in PARP1/AIF and nuclear Akt signaling pathways or metastasis processes were validated by western blotting. Conclusion: TP exerted anti-tumor activity on non-small cell lung cancer (NSCLC) A549 lung adenocarcinoma cells by dysregulating tumor-related protein expression. Herein, we provide a preliminary study of TP-related cytotoxicity on A549 cells using proteomics tools. These findings may improve the current understanding of the anti-tumor effects of TP on lung cancer cells and may reveal candidate proteins as potential targets for the treatment of lung cancer.

scholarly journals Myrcene Exhibits Antitumor Activity Against Lung Cancer Cells by Inducing Oxidative Stress and Apoptosis Mechanisms

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2096118
Author(s):  
Xudong Bai ◽  
Jin Tang
Keyword(s):  
Oxidative Stress ◽  
Lung Cancer ◽  
Dna Damage ◽  
Cell Death ◽  
Cancer Cells ◽  
Metabolic Activity ◽  
Lung Cancer Cells ◽  
Adenocarcinoma Cells ◽  
Lung Adenocarcinoma Cells ◽  
A549 Lung

Myrcene, a natural olefinic hydrocarbon, possesses anti-inflammatory, analgesic, antibiotic, and antimutagenic properties, but its anticancer effect has not yet been elucidated. Hence, the present study was framed to investigate the molecular mechanism by which myrcene mediates the anticancer activity of A549 lung adenocarcinoma cells. In vitro, A549 lung cancer cells were cultured either with or without myrcene, and the effects on cellular metabolic activity, levels of reactive oxygen species (ROS), mitochondrial integrity, deoxyribonucleic acid (DNA) damage, and activity of caspases were analyzed. The study demonstrated that compared with control cells, myrcene induces cell death in a dose-dependent manner while inducing ROS levels. Further experiments revealed that the metabolic activity of the A549 lung adenocarcinoma cells was diminished with increased DNA damage and altered cellular integrity. In addition, increased activity of caspase-3 was also evidenced with reduced mitochondrial membrane potential synthesis in the myrcene-treated cells, which demonstrate that lung cancer cells experience signs of toxicity during myrcene treatment through the activation of the apoptosis mechanism via mitochondria-mediated cell death signaling and induction of oxidative stress. The results provide the first report on the evidence of anticancer activity and the possibility of a new drug that could be used for the treatment of lung cancer.

Enhancing effect of connexin 32 gene on vinorelbine-induced cytotoxicity in A549 lung adenocarcinoma cells

2007 ◽  
Vol 60 (3) ◽  
pp. 449-457 ◽  
Author(s):  
Hiromi Sato ◽  
Keiko Fukumoto ◽  
Sachio Hada ◽  
Hiromi Hagiwara ◽  
Eriko Fujimoto ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Connexin 32 ◽  
Enhancing Effect ◽  
Adenocarcinoma Cells ◽  
Lung Adenocarcinoma Cells ◽  
A549 Lung
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