The Bank of Biological Samples by Seversk Biophysical Research Center

2020 ◽  
Vol 65 (2) ◽  
pp. 21-26
Author(s):  
T. Takhauova ◽  
D. Isubakova ◽  
E. Bronikovskaya ◽  
O. Tsymbal ◽  
M. Khalyuzova ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Radiation Exposure ◽  
Biological Material ◽  
White Blood Cells ◽  
Nuclear Industry ◽  
Healthy People ◽  
Research Center ◽  
Malignant Neoplasms ◽  
Siberian Chemical

Purpose: Description of the collection, structure and development dynamics during the period from 2015 to 2019 of the biological material bank of the Seversk Biophysical Research Center, as well as research works carried out using biological material from its collection. Material and methods: The collection of bank of biological material includes the following types of biological material: venous blood, genomic DNA of white blood cells, cytogenetic suspensions of blood lymphocytes, surgical, biopsy and autopsy material. Biological material was obtained from people exposed to chronic radiation exposure: employees of the nuclear industry and the population permanently residing in the territory located in the area of the nuclear industry. Results: At present, the collection of the biological material bank contains more than 21,000 samples of biological material obtained from more than 8,000 donors. The collection of bank of biological material is represented by 4 main blocks: biological material of conditionally healthy employees of company of the nuclear industry (Siberian Chemical Combine); biological material of conditionally healthy people permanently residing in the territory located in the area of the nuclear industry enterprise (Seversk); biological material of patients (workers of the Siberian Chemical Combine and residents of Seversk) with malignant neoplasms and biological material of patients (workers of the Siberian Chemical Combine and residents of Seversk) with acute myocardial infarction. In the collection of bank of biological material, the proportion of samples of biological material of relatively healthy workers of the Siberian Chemical Combine is 33 %, residents of Seversk — 40.8 %, patients with malignant neoplasms — 22.2 %, patients with acute myocardial infarction — 4 %. Conclusion: Biological material bank Seversk Biophysical Research Center is unique collection of biological material samples of healthy people and patients with socially significant diseases. A feature of the biological material of the biological material bank is that it is obtained from people who have been exposed to chronic low-intensity radiation exposure and can be used to evaluate its radiogenic effects.

scholarly journals Serum Procalcitonin as a Marker for Infection in Patients with Acute Myocardial Infarction

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S352-S352
Author(s):  
Itzhak Vitkon-Barkay ◽  
Tsilia Lazarovitch ◽  
Dror Marchaim ◽  
Hannah Segaloff ◽  
Ronit Zaidenstein ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Predictive Value ◽  
Bacterial Infections ◽  
Medical Center ◽  
White Blood Cells ◽  
Laboratory Data ◽  
Significant Proportion ◽  
Infection Diagnosis ◽  
Wbc Count

Abstract Background Significant proportion of patients with acute myocardial infarction (AMI) also present with systemic inflammatory response syndrome (SIRS). Thus it is difficult to determine in certain situations, whether empiric antibiotic treatment is warranted. Serum procalcitonin (PCT) is known to be elevated in bacterial infections, but its performances in predicting bacterial infection among patients with AMI, who might benefit from appropriate empiric management, is unknown. Methods A prospective observational study was conducted at Assaf Harofeh Medical Center, Israel. Serum PCT was collected within 48 hours from patients presenting with AMI. Demographic, clinical, and laboratory data, were collected prospectively. Two experienced Infectious Diseases (ID) specialists who were blinded to the PCT results, independently determined the gold standard for infection in every patient. By utilizing sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and the area under the ROC curve (AUC), the performance of PCT, fever, white-blood cells (WBC) count and C-reactive protein (CRP) for infection diagnosis was calculated. Results The analysis included 230 AMI patients (age 63.0 ± 13.0 years), of which 36 (15.6%) were determined to be infected. The best cutoff for PCT as a differentiating marker between infected and non-infected patients was achieved at 0.09ng/dl (sensitivity 94.4%, specificity 85.1%, AUC ROC 0.94). This test outperformed CRP, WBC, and fever, for infection diagnosis (figure). Conclusion PCT should be utilized for ruling out infection in AMI patients by utilizing serum PCT>0.09ng/dl (i.e., ≥0.1ng/dl) as a cutoff. Disclosures All authors: No reported disclosures.

scholarly journals The risk of acute myocardial infarction development in long-term radiation exposure

2012 ◽  
Vol 11 (2) ◽  
pp. 146-152
Author(s):  
A. B. Karpov ◽  
R. M. Takhauov ◽  
Yu. V. Semyonova ◽  
T. M. Litvinenko ◽  
D. Ye. Kalinkin ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Radiation Exposure

Downregulated Expression of TRPV2 in Peripheral Blood Cells following Acute Myocardial Infarction Is Inversely Correlated with Serum Levels of CRP and Troponin I

Cardiology ◽  
2018 ◽  
Vol 139 (3) ◽  
pp. 169-174 ◽  
Author(s):  
Zach Rozenbaum ◽  
Lena Cohen ◽  
Einat Bigelman ◽  
Yacov Shacham ◽  
Gad Keren ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Transient Receptor Potential ◽  
Mononuclear Cells ◽  
Troponin I ◽  
Gradient Centrifugation ◽  
White Blood Cells ◽  
Transient Receptor Potential Vanilloid

Objectives: We have recently shown that the transient receptor potential vanilloid 2 (TRPV2) channel is exclusively upregulated in rat/murine peri-infarct monocytes/macrophages following an acute myocardial infarction (AMI), and that this overexpression might be detrimental for cardiac recovery. We aimed to characterize the expression levels of TRPV2 in peripheral blood mononuclear cells (PBMCs) of AMI patients relative to individuals with normal coronaries, and to analyze potential associations with inflammatory and cardiac ischemic markers. Methods: Patients who underwent coronary angiography due to AMI or chest pain were prospectively included. PBMCs were isolated from whole blood by Ficoll gradient centrifugation. TRPV2 expression was analyzed by real-time PCR. C-reactive protein (CRP) and troponin I (TpI) levels were determined at the central chemistry laboratory; interleukin 6 and insulin-like growth factor (IGF)-1 levels were tested by ELISA. Results: Following AMI, the number of TRPV2-expressing PBMCs was reduced when compared to in patients with normal coronaries. An inverse correlation was documented between the numbers of circulating macrophages and TRPV2 expression. Additionally, TRPV2 expression was inversely correlated with CRP and TpI and directly correlated with serum IGF-1. Conclusions: We assume that peripheral TRPV2 downregulation occurs concomitantly with the accumulation of TRPV2-white blood cells in the peri-infarct zone. TRPV2 may thus represent a novel target for treatment in the acute phase after MI.

scholarly journals Changes in the flow properties of white blood cells after acute myocardial infarction.

Heart ◽  
1989 ◽  
Vol 62 (5) ◽  
pp. 329-334 ◽  
Author(s):  
G B Nash ◽  
B Christopher ◽  
A J Morris ◽  
J A Dormandy
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Blood Cells ◽  
White Blood Cells ◽  
Flow Properties

scholarly journals Ionizing Radiation Exposure to Patients Admitted With Acute Myocardial Infarction in the United States

Circulation ◽  
2010 ◽  
Vol 122 (21) ◽  
pp. 2160-2169 ◽  
Author(s):  
Prashant Kaul ◽  
Sofia Medvedev ◽  
Samuel F. Hohmann ◽  
Pamela S. Douglas ◽  
Eric D. Peterson ◽  
...  
Keyword(s):  
United States ◽  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Ionizing Radiation ◽  
Radiation Exposure ◽  
The United States ◽  
Ionizing Radiation Exposure ◽  
Radiation Exposure To Patients

T lymphocytes alterations are associated with oxidized LDL, troponin T, white blood cells and C-reactive protein during acute myocardial infarction

2014 ◽  
Vol 56 (1) ◽  
pp. 57-66
Author(s):  
Patrícia Napoleão ◽  
Mafalda Selas ◽  
Cláudia Freixo ◽  
Miguel Mota Carmo ◽  
Ana Maria Viegas-Crespo ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
T Lymphocytes ◽  
Blood Cells ◽  
Troponin T ◽  
Oxidized Ldl ◽  
White Blood Cells ◽  
C Reactive Protein ◽  
Reactive Protein

T lymphocytes alterations are associated with oxidized LDL, troponin T, white blood cells and C-reactive protein during acute myocardial infarction

2013 ◽  
Vol 55 (3) ◽  
pp. 349-358 ◽  
Author(s):  
Patrícia Napoleão ◽  
Mafalda Selas ◽  
Cláudia Freixo ◽  
Miguel Mota Carmo ◽  
Ana Maria Viegas-Crespo ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
T Lymphocytes ◽  
Blood Cells ◽  
Troponin T ◽  
Oxidized Ldl ◽  
White Blood Cells ◽  
C Reactive Protein ◽  
Reactive Protein

An electron microscopic study of the intra-myocardial lesions in cases of acute myocardial infarction

1978 ◽  
Vol 36 (2) ◽  
pp. 484-485
Author(s):  
Masahiro Ono ◽  
Kaoru Aihara ◽  
Gompachi Yajima
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Coronary Vessels ◽  
Vascular Wall ◽  
Vascular Changes ◽  
Electron Microscopic ◽  
Main Trunk ◽  
Mechanical Destruction ◽  
Transmission Electron ◽  
Myocardial Lesions

The pathogenesis of the arteriosclerosis in the acute myocardial infarction is the matter of the extensive survey with the transmission electron microscopy in experimental and clinical materials. In the previous communication,the authors have clarified that the two types of the coronary vascular changes could exist. The first category is the case in which we had failed to observe no occlusive changes of the coronary vessels which eventually form the myocardial infarction. The next category is the case in which occlusive -thrombotic changes are observed in which the myocardial infarction will be taken placed as the final event. The authors incline to designate the former category as the non-occlusive-non thrombotic lesions. The most important findings in both cases are the “mechanical destruction of the vascular wall and imbibition of the serous component” which are most frequently observed at the proximal portion of the coronary main trunk.

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