THE STATE OF HUMORAL IMMUNITY IN PAIRS “MOTHER-CHILD” AT INFLUENZA A(H3N2) IN THE FIRST TRIMESTER OF PREGNANCY

2016 ◽  
Vol 1 (61) ◽  
pp. 66-68
Author(s):  
Луценко ◽  
Mikhail Lutsenko ◽  
Гориков ◽  
Igor Gorikov ◽  
Андриевская ◽  
...  
Keyword(s):  
Antibody Titer ◽  
Influenza A ◽  
Influenza Infection ◽  
First Trimester ◽  
Full Term ◽  
Fourth Group ◽  
The Third ◽  
Umbilical Blood ◽  
Antibody Titers ◽  
Somatic Diseases

At full-term pregnancy the level of anti-influenza antibodies in 126 pairs “mother-child” at influenza A(H3N2) in the first trimester of gestation was studied. The first group (comparison) was made of 40 pairs “mother-child” at pregnancy not complicated with acute respiratory virus infection, but with exacerbation of somatic diseases as well as moderate and severe somatic and obstetric pathology. The second group consisted of 43 pairs “mother-child” with influenza infection with antibody titer 1:4-1:16; the third group had 41 pairs “mother-child” with influenza А(Н3N2) with antibody titer 1:8-1:32; the fourth group had 42 pairs “mother-child” with influenza infection (antibody titers were 1:32-1:128) associated with the threat of miscarriage at early stages of gestation. It was found out that in the second group in 80% of cases mothers had higher titers of anti-virus antibodies in comparison with the titers of their children; in 12.5% of cases there were equal titers of antibodies in maternal and umbilical blood. In the second and the third groups unlike the first one there was a tendency to the decrease of a number of pairs that had higher titers of antibodies in mothers and there was an increase of pairs with equal titers of anti-virus antibodies. In the fourth group the number of pairs with domination of titers of anti-influenza antibodies increased till 28.6% in children in comparison with the titers in their mothers (in the second group it was in 4.7%, р<0.05), which suggested antenatal infecting of full term newborns.

ULTRASOUND CHARACTERISTIC OF THYMUS GLAND IN MATURE NEWBORNS FROM MOTHERS WITH INFLUENZA A(H3N2) IN THE FIRST TRIMESTER OF PREGNANCY

2016 ◽  
Vol 1 (62) ◽  
pp. 68-71
Author(s):  
Игорь Гориков ◽  
Igor Gorikov ◽  
Михаил Луценко ◽  
Mikhail Lutsenko
Keyword(s):  
Influenza Virus ◽  
Antibody Titer ◽  
Influenza A ◽  
First Trimester ◽  
Thymus Gland ◽  
Fourth Group ◽  
Influenza Virus A ◽  
The Third ◽  
Early Stages ◽  
First Trimester Of Pregnancy

Ultrasound characteristic of thymus gland was studied in 109 mature newborns at the 3-5th days of life. All the examined patients were divided into 4 groups. The first group (control) included 30 healthy newborns of 38-40 weeks from mothers with physiologic course of pregnancy. The second group had 28 newborns whose mothers at early stages of gestation suffered influenza virus А(Н3N2) with antibody titer 1:4-1:16; in the third group there were 26 children of 38-40 weeks from mothers who suffered in the first trimester of pregnancy influenza virus A(H3N2) with antibody titer 1:8-1:32; the fourth group consisted of 25 newborns from mothers who suffered at early stages of gestation influenza virus A(H3N2) with antibody titer 1:32-1:128. The children in the second and the third group in comparison with the first one did not have any changes of organometric parameters of the immune organ. In the newborns of the fourth group the length of the thymus gland decreased till 3.8±0.11 cm, the width till 2.4±0.06 cm, the mass till 8.6±0.53 g and the volume of the organ till 6.0±0.37 sm3. In the first group these parameters were 4.2±0.09 cm (р<0.05), 2.8±0.05 cm (р<0.001), 10.5±0.37 g (р<0.01), 7.4±0.25 sm3 (р<0.01), respectively. This suggests the suppression of organogenesis of thymus in children with antenatal anamnesis burdened with influenza virus А(Н3N2) with antibody titer 1:32-1:128 in their mothers at early stages of gestation

SYSTEM INFLAMMATORY RESPONSE AT THE EXACERBATION OF CHRONIC SIMPLE BRONCHITIS CAUSED BY INFLUENZA VIRUS B IN WOMEN IN THE FIRST TRIMESTER OF PREGNANCY

2016 ◽  
Vol 1 (60) ◽  
pp. 67-69
Author(s):  
Смирнова ◽  
Tatyana Smirnova ◽  
Резник ◽  
Vadim Reznik ◽  
Одиреев ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Inflammatory Cytokines ◽  
First Trimester ◽  
Fourth Group ◽  
The Third ◽  
Early Stages ◽  
Tnf Α ◽  
Antibody Titers ◽  
Anti Inflammatory ◽  
Pro Inflammatory Cytokines

The contents of anti-inflammatory and pro-inflammatory cytokines in 95 women in the first trimester of gestation were studied. The first (control) group included 25 women with physiologic course of pregnancy; the second group consisted of 25 patients with exacerbation of chronic simple bronchitis caused by influenza virus B (antibody titers were 1:16-1:64); the third group consisted of 24 pregnant women with exacerbation of chronic simple bronchitis under influenza virus В (antibody titers were 1:32-1:128); the fourth group consisted of 21 women with exacerbation of chronic simple bronchitis induced by influenza virus B (antibody titers were 1:64-1:256) at early stages of gestation. In the patients of the second group in comparison with the patients of the first group there was an increase of IL-4 till 19.7±2.17 pg/ml, of TNF-α till 33.4±3.09 pg/ml and of INF-γ till 30.9±2.64 pg/ml (in the control it was 12.9±1.16 pg/ml, р<0.01; 24.7±2.27 pg/ml, р<0.05 and 22.3±2.08 pg/ml, р<0.05, respectively). In the third group in comparison with the second group there was no growth of anti-inflammatory and pro-inflammatory cytokines. In the fourth group in comparison with the second group there was the biggest increase of IL-4 till 27.7±2.42 pg/ml (р<0.05), of TNF-α till 43.6±2.79 pg/ml (p<0.05) and of INF-γ till 40.7±2.96 pg/ml (p<0.05). This suggest the paramount importance of the growth of anti-virus antibodies titers in the pathogenesis of exacerbation of chronic simple bronchitis under influenza virus B in women at early stages of gestation.

THE STATE OF HEPATOBILIARY SYSTEM IN TERM NEWBORNS FROM MOTHERS WHO SUFFERED INFLUENZA VIRUS A(H3N2) IN THE FIRST TRIMESTER OF PREGNANCY

2016 ◽  
Vol 1 (62) ◽  
pp. 72-75
Author(s):  
Наталия Ишутина ◽  
Nataliya Ishutina ◽  
Игорь Гориков ◽  
Igor Gorikov ◽  
Ирина Андриевская ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Total Cholesterol ◽  
Total Protein ◽  
Influenza A ◽  
Influenza Infection ◽  
Direct Bilirubin ◽  
Control Group ◽  
Umbilical Blood ◽  
Antibody Titers ◽  
Term Newborns

The functional state of liver in 112 term newborns, who depending on their antenatal ontogenesis were divided into 4 groups, was studied. The first group (control) was made of 30 healthy newborns from mothers with physiological course of pregnancy; the second group consisted of 30 children whose mothers suffered influenza A(H3N2) (antibody titers were 1:4-1:16), the third group had 27 newborns with antenatal anamnesis complicated with influenza A(H3N2) (antibody titers were 1:8-1:32) and the fourth group had 25 children whose mothers suffered A(H3N2) (antibody titers were 1:32-1:128) at early stages of gestation. In newborns in the serum of the umbilical blood there were found the contents of total protein, albumins, total, indirect and direct bilirubin, activity of alanine aminotransferase, serum glutamate-oxaloacetate-transaminase, gamma-glutamyl transpeptidase, lactic dehydrogenase and alkaline phosphatase, concentration of total cholesterol, lipoproteins of low density and triglycerides. In babies from mothers who suffered influenza infection with antibody titers 1:32-1:128 there was the decrease of total protein till 55.1±1.27 g/l (in the control group it was 62.1±1.45 g/l; р<0.01) and albumins till 34.2±1.01 g/l (in the control it was 38.2±1.13 g/l; р<0.05) against the increase of total bilirubin till 35.5±1.29 mcmole/l (in the control it was 25.3±1.67 g/l; р<0.001), of indirect bilirubin till 33.2±1.28 mcmole/l (in the control it was 23.4±1.67 mcmole/l; р<0.001), of direct bilirubin till 2.33±0.10 mcmole/l (in the control it was 1.91±0.09; р<0.01) and the activity of alkaline phosphatase till 175.7±10.62 МЕ/l (in the control it was 141.6±6.39 МЕ/l; р<0.01). This showed the suppression of protein-synthesizing and pigment function of the liver as well as of the process of cholestasis. At the same time there was the decrease of total cholesterol till 1.83±0.06 mmole/l (in the control it was 2.08±0.07 mmole/l; р<0.01) and lipoproteins of high density till 1.02±0.06 mmole/l (in the control it was 1.27±0.06 mmole/l; р<0.01). Their concentration causes the stability of cellular membranes and hypothalamic-pituitarium-adrenal system in newborns to unfavourable factors in the early postnatal period of their development.

THE CHANGES IN CYTOKINS LEVEL AT THE EXACERBATION OF CHRONIC SIMPLE BRONCHITIS CAUSED BY VIRUS OF PARAINFLUENZA OF THE THIRD TYPE IN WOMEN IN THE FIRST TRIMESTER OF PREGNANCY

2016 ◽  
Vol 1 (62) ◽  
pp. 64-67
Author(s):  
Артем Колосов ◽  
Artem Kolosov ◽  
Татьяна Смирнова ◽  
Tatyana Smirnova ◽  
Вадим Резник ◽  
...  
Keyword(s):  
Antibody Titer ◽  
First Trimester ◽  
Interleukin 4 ◽  
Control Group ◽  
Fourth Group ◽  
The Third ◽  
Early Stages ◽  
Tnf Α ◽  
First Trimester Of Pregnancy ◽  
Ifn Γ

The contents of interleukin-4 (IL-4), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in the blood serum were studied in 89 women in the first trimester of pregnancy. The first group (control) was made out of 25 women with uncomplicated pregnancy; the second had 23 women with the exacerbation of chronic simple bronchitis caused by parainfluenza of the 3rd type with antibody titer 1:16-1:64; the third one consisted of 21 patients with the exacerbation of chronic simple bronchitis induced by parainfluenza infection with antibody titers 1:32-1:128; the fourth group had 20 women with exacerbation of chronic simple bronchitis of parainfluenza etiology with antibody titer 1:64-1:256 at early stages of gestation. In the second group in comparison with the first one there were higher contents of IL-4: 21.1±2.00 pg/ml and INF-γ: 29.5±2.31 pg/ml than in the control group: 12.9±1.16 pg/ml (р<0.001) and 22.3±2.08 pg/ml (р<0.05), respectively. In the third group in comparison with the first one there were higher values of IL-4 (the growth by 132.6%, р<0.001), TNF-α (by 36%, p<0.01) and IFN-γ (by 43%, p<0.01). The patients in the fourth group in comparison with the third one did not have significant differences in TNF-α and IFN-γ. Buy there was the decrease of the level of IL-4 by 11.3% (р<0.05) that shows the imbalance of cytokines in women with exacerbation of chronic simple bronchitis caused by parainfluenza of the third type with antibody titer 1:64-1:256 at early stages of gestation. This may lead to persistent course of infectious-inflammatory process as well as to autoimmune damage of vital organs and the formation of initial placental insufficiency.

THE PASSIVE TRANSFER OF ANTIBODIES TO ESCHERICHIA COLI 0111:B4 FROM MOTHER TO OFFSPRING

PEDIATRICS ◽  
1961 ◽  
Vol 27 (2) ◽  
pp. 308-313
Author(s):  
Sidney Sussman
Keyword(s):  
Escherichia Coli ◽  
Antibody Titer ◽  
Passive Transfer ◽  
The Third ◽  
Coli Antibody ◽  
Antibody Titers

Esch. coli antibody titers in 27 mothers and their respective offspring were studied by the trypsinated and nontrypsinated hemagglutination technic. All of the maternal sera and colostra contained Esch. coli 0111-B4 antibody. In 19 cases the antibody titer in the specimens of colostrum on the first day was higher than that of the corresponding sera. The antibody titer in the colstrum fell rapidly during the next 3 to 4 days. Five cord sera had a low antibody titer to Esch. coli 0111:B4 when tested by the trypsinated hemagglutination method. By contrast, only two cord sera were positive for Esch. coli 0111:B4 antibody when tested by the untrypsinated hemagglutination technic. With the trypsinated method, two infants showed a 2-tube rise and one infant had a 1-tube rise in titer at the end of the third colostrum day; one infant demonstrated a 1-tube rise in titer when tested by the untrypsinated hemagglutination technic. In general, there was a 1-to-3-tube difference between the trypsinated and untrypsinated hemagglutination procedures.

THE STATE OF THE IMMUNE SYSTEM IN WOMEN IN THE SECOND TRIMESTER OF PREGNANCY AT EXACERBATION OF CHRONIC SIMPLE BRONCHITIS CAUSED BY INFLUENZA A(H3N2)

2017 ◽  
pp. 7-7
Author(s):  
Леонид Нахамчен ◽  
Leonid Nakhamchen ◽  
Игорь Гориков ◽  
Igor Gorikov ◽  
Виктор Колосов ◽  
...  
Keyword(s):  
Optical Density ◽  
Influenza A ◽  
Immunoglobulin A ◽  
Second Trimester ◽  
Local Immunity ◽  
The Third ◽  
Persistent Virus Infection ◽  
Antibody Titers ◽  
Persistent Virus ◽  
Secretory Immunoglobulin

The contents of anti-influenza antibodies, secretory immunoglobulin A (sIgA) and circulating immune complexes (CIC) were studied in 145 women in the second trimester of pregnancy. All women were divided into 5 groups. The first group included 30 women with the physiologic course of pregnancy. The second one consisted of 30 patients with chronic simple bronchitis (CB) in remission. The third group had 30 patients with the exacerbation of CB caused by influenza A(H3N2) (antibody titers were 1:16-1:64); the fourth group included 30 patients with CB, whose exacerbation was caused by influenza (antibody titers were 1:32-1:128). It was found out that in the second group there were higher concentrations of sIgA (7.34±0.37 mg/L) and CIC (0.191±0.006 units of optical density), than in the first group (5.86±0.43 mg/L, p<0.05 and 0.095±0.003 units of optical density, respectively, p<0.001). It can probably be explained by the stimulating influence of persistent virus infection on the local immunity and its important role in the development of autoimmune responses in pregnant women. In the fifth group unlike the third group there were registered minimal contents of sIgA: 3.86±0.25 mg/L (p<0.001) and maximal level of CIC: 0.232±0.006 units of optical density (p<0.001). The revealed changes suggested the decrease of local immune protection of airways and autoimmune alteration of a woman’s body caused by high intensity of humoral immunity to influenza virus A(H3N2) under exacerbation of chronic simple bronchitis in patients in the second trimester of gestation.

THE CHANGE OF HORMONAL STATUS IN WOMEN WITN INFLUENZA VIRUS A(H3N2) IN THE FIRST TRIMESTER OF PREGNANCY

2016 ◽  
Vol 1 (60) ◽  
pp. 80-84
Author(s):  
Луценко ◽  
Mikhail Lutsenko ◽  
Гориков ◽  
Igor Gorikov ◽  
Андриевская ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Influenza Infection ◽  
First Trimester ◽  
Negative Influence ◽  
Placental Lactogen ◽  
Premature Delivery ◽  
Hormonal Status ◽  
Child Bearing ◽  
Antibody Titers ◽  
First Trimester Of Pregnancy

The contents of β-subunit of chorionic gonadotropin (β-CG), estriol, progesterone, dehydroepiandrosterone (DEAS), placental lactogen and cortisol were studied in 164 women of child-bearing age at the 7-9th weeks of pregnancy. The first group (control) consisted of 30 women with physiologic course of pregnancy. The second group (comparison) had 32 patients with the threat of premature delivery of non-infectious etiology. The third group included 36 patients with influenza virus А(Н3N2) (anti-virus antibody titers were 1:4-1:16); the fourth group had 34 women with influenza virus А(Н3N2) (antibody titers were 1:8-1:32); in the fifth group there were 32 patients with influenza virus А(Н3N2) (antibody titers were 1:32-1:128) and the threat of premature delivery in the period of reconvalescence. The patients of the fifth group had the decrease of the level of β-CG till 40.5±2.0 mМЕ/ml, of estriol till 3.4±0.21 nmole/l, of progesterone till 35.1±3.13 nmole/l, of DEAS till 7.3±0.68 nmole/l, of placental lactogen till 12.1±1.0 nmole/l, as well as the increase of cortisol till 738.7±22.08 nmole/l in comparison with 73.0±2.0 mМЕ/ml (р&#60;0.001), 5.9±0.33 nmole/l (р&#60;0.001), 70.6±4.14 nmole/l (р&#60;0.001); 7.3±0.68 nmole/l (р&#60;0.001), 12.1±1.0 nmole/l (р&#60;0.001) and 567.8±24.02 nmole/l (р&#60;0.001) in the first group, respectively. The differences of hormonal status in the fifth and third group consisted of the drop of concentration of β-CG by 22.8% (р&#60;0.001), of estriol by 26.1% (р&#60;0.01), of progesterone by 32.1% (р&#60;0.001) and placental lactogen by 32.4% (р&#60;0.001). At the same time there were no significant changes in the concentration of DEAS and there were registered higher indicators of cortisol, i.e. the increase by 11.5% (р&#60;0.05), which suggested the significance of the growth of antivirus antibody titers in the suppression of synthesis of hormones in ovaries and in syncytiotrophoblast as a result of direct and indirect negative influence of influenza infection.

scholarly journals Comparison of Serum Hemagglutinin and Neuraminidase Inhibition Antibodies After 2010–2011 Trivalent Inactivated Influenza Vaccination in Healthcare Personnel

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Maryrose R. Laguio-Vila ◽  
Mark G. Thompson ◽  
Sue Reynolds ◽  
Sarah M. Spencer ◽  
Manjusha Gaglani ◽  
...  
Keyword(s):  
Influenza Vaccine ◽  
Influenza A ◽  
Influenza Infection ◽  
Fold Increase ◽  
Healthcare Personnel ◽  
Virus Infections ◽  
Influenza A Viruses ◽  
Duration Of Illness ◽  
Antibody Titers ◽  
Influenza Vaccinations

Abstract Background.  Most inactivated influenza vaccines contain purified and standardized hemagglutinin (HA) and residual neuraminidase (NA) antigens. Vaccine-associated HA antibody responses (hemagglutination inhibition [HAI]) are well described, but less is known about the immune response to the NA. Methods.  Serum of 1349 healthcare personnel (HCP) electing or declining the 2010–2011 trivalent-inactivated influenza vaccine ([IIV3], containing A/California/7/2009 p(H1N1), A/Perth/16/2009 [H3N2], B/Brisbane/60/2008 strains) were tested for NA-inhibiting (NAI) antibody by a modified lectin-based assay using pseudotyped N1 and N2 influenza A viruses with an irrelevant (H5) HA. Neuraminidase-inhibiting and HAI antibody titers were evaluated approximately 30 days after vaccination and end-of-season for those with polymerase chain reaction (PCR)-confirmed influenza infection. Results.  In 916 HCP (68%) receiving IIV3, a 2-fold increase in N1 and N2 NAI antibody occurred in 63.7% and 47.3%, respectively. Smaller responses occurred in HCP age &gt;50 years and those without prior 2009–2010 IIV3 nor monovalent A(H1N1)pdm09 influenza vaccinations. Forty-four PCR-confirmed influenza infections were observed, primarily affecting those with lower pre-exposure HAI and NAI antibodies. Higher pre-NAI titers correlated with shorter duration of illness for A(H1N1)pdm09 virus infections. Conclusions.  Trivalent-inactivated influenza vaccine is modestly immunogenic for N1 and N2 antigens in HCP. Vaccines eliciting robust NA immune responses may improve efficacy and reduce influenza-associated morbidity.

The Assessment of the Umbilical Blood Flow of the Surviving Twin after the Intrauterine Death of the Other Twin

1996 ◽  
Vol 45 (3) ◽  
pp. 383-386 ◽  
Author(s):  
W. Malinowski ◽  
W. Dec ◽  
I. Biskup
Keyword(s):  
First Trimester ◽  
The Other ◽  
Intrauterine Death ◽  
Second Trimester ◽  
Third Trimester ◽  
Venous Flow ◽  
The Third ◽  
Diastolic Flow ◽  
Umbilical Blood ◽  
Umbilical Blood Flow

AbstractThis paper summarizes our experience with Doppler velocimetry in survivors of intrauterine co-twin demise. In the first trimester, ten dichorionic deaths occurred; none of the survivors developed flow disorders. During the second trimester, there were three intrauterine demises, two of them were monochorionic and the survivors developed flow disorders: one presented transitory venous flow aberration, the other one an impaired development of diastolic flow. In the third trimester, two intrauterine deaths occurred. One case of twin to twin transfusion syndrome (TTTS) was complicated by the donor's death and the recipient showed a loss of diastolic flow. The second one happened during a dichorionic twin pregnancy. The survivor presented high systolic/diastolic daily ratio (S/D = 7.8).

scholarly journals Evaluation of correlates of protection against influenza A(H3N2) and A(H1N1)pdm09 infection: Applications to the hospitalized patient population

2018 ◽  
Author(s):  
Joshua G. Petrie ◽  
Emily T. Martin ◽  
Rachel Truscon ◽  
Emileigh Johnson ◽  
Caroline K. Cheng ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Influenza A ◽  
Influenza Infection ◽  
Geometric Mean ◽  
Influenza Vaccines ◽  
Respiratory Illnesses ◽  
Illness Onset ◽  
Correlates Of Protection ◽  
Antibody Titers ◽  
H3n2 Vaccine

ABSTRACTBackgroundInfluenza vaccines are important for prevention of influenza-associated hospitalization. Assessments of serologic correlates of protection can support interpretation of influenza vaccine effectiveness evaluations in hospitalized populations.MethodsSerum specimens collected at admission from adults hospitalized for treatment of acute respiratory illnesses during two influenza seasons were tested in hemagglutination-inhibition (HAI) and neuraminidase-inhibition (NAI) assays. We evaluated the suitability of these specimens as proxies for pre-infection immune status, and measured associations between antibody titers and influenza vaccination and infectionResultsSpecimens were collected within 3 days of illness onset from 65% of participants; geometric mean titers (GMTs) did not vary by day of collection. In both seasons, vaccinated participants had higher HAI and NAI GMTs than unvaccinated participants. HAI titers against the 2014-2015 A(H3N2) vaccine strain did not correlate with protection from infection with antigenically-drifted A(H3N2) viruses that circulated that season. In contrast, higher HAI titers against the A(H1N1)pdm09 vaccine strain were associated with reduced odds of A(H1N1)pdm09 infection in 2015-2016.ConclusionsSerum collected after hospital admission can be used to assess correlates of protection against influenza infection. Broader implementation of similar studies would provide an opportunity to understand the successes and shortcomings of current influenza vaccines.

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