scholarly journals Antimicrobial Susceptibility, Tetracycline and Erythromycin Resistance Genes, and Multilocus Sequence Typing of Streptococcus suis Isolates from Diseased Pigs in China

2013 ◽  
Vol 75 (5) ◽  
pp. 583-587 ◽  
Author(s):  
Lei CHEN ◽  
Yajing SONG ◽  
Zigong WEI ◽  
Hongkui HE ◽  
Anding ZHANG ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Antimicrobial Susceptibility ◽  
Multilocus Sequence Typing ◽  
Streptococcus Suis ◽  
Erythromycin Resistance

scholarly journals Antimicrobial susceptibility, multilocus sequence typing, and virulence of listeria isolated from a slaughterhouse in Jiangsu, China

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Liting Wu ◽  
Hongduo Bao ◽  
Zhengquan Yang ◽  
Tao He ◽  
Yuan Tian ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Antimicrobial Susceptibility ◽  
Multilocus Sequence Typing ◽  
Tetracycline Resistance ◽  
Dilution Method ◽  
Processing Plant ◽  
Meat Processing ◽  
Processing Plants

Abstract Background Listeria monocytogenes is one of the deadliest foodborne pathogens. The bacterium can tolerate severe environments through biofilm formation and antimicrobial resistance. This study aimed to investigate the antimicrobial susceptibility, resistance genes, virulence, and molecular epidemiology about Listeria from meat processing environments. Methods This study evaluated the antibiotic resistance and virulence of Listeria isolates from slaughtering and processing plants. All isolates were subjected to antimicrobial susceptibility testing using a standard microbroth dilution method. The harboring of resistant genes was identified by polymerase chain reaction. The multilocus sequence typing was used to determine the subtyping of the isolates and characterize possible routes of contamination from meat processing environments. The virulence of different STs of L. monocytogenes isolates was evaluated using a Caco-2 cell invasion assay. Results A total of 59 Listeria isolates were identified from 320 samples, including 37 L. monocytogenes isolates (62.71%). This study evaluated the virulence of L. monocytogenes and the antibiotic resistance of Listeria isolates from slaughtering and processing plants. The susceptibility of these 59 isolates against 8 antibiotics was analyzed, and the resistance levels to ceftazidime, ciprofloxacin, and lincomycin were as high as 98.31% (L. m 37; L. innocua 7; L. welshimeri 14), 96.61% (L. m 36; L. innocua 7; L. welshimeri 14), and 93.22% (L. m 35; L. innocua 7; L. welshimeri 13), respectively. More than 90% of the isolates were resistant to three to six antibiotics, indicating that Listeria isolated from meat processing environments had high antimicrobial resistance. Up to 60% of the isolates harbored the tetracycline-resistance genes tetA and tetM. The frequency of ermA, ermB, ermC, and aac(6′)-Ib was 16.95, 13.56, 15.25, and 6.78%, respectively. Notably, the resistant phenotype and genotype did not match exactly, suggesting that the mechanisms of antibiotic resistance of these isolates were likely related to the processing environment. Multilocus sequence typing (MLST) revealed that 59 Listeria isolates were grouped into 10 sequence types (STs). The dominant L. monocytogenes STs were ST5, ST9, and ST121 in the slaughtering and processing plant of Jiangsu province. Moreover, ST5 subtypes exhibited high invasion in Caco-2 cells compared with ST9 and ST121 cells. Conclusion The dominant L. monocytogenes ST5 persisted in the slaughtering and processing plant and had high antimicrobial resistance and invasion characteristics, illustrating a potential risk in food safety and human health.

Capsular serotypes, antimicrobial susceptibility, and the presence of transferable oxazolidinone resistance genes in Streptococcus suis isolated from healthy pigs in China

2020 ◽  
Vol 247 ◽  
pp. 108750
Author(s):  
Chaoyang Zhang ◽  
Peng Zhang ◽  
Yao Wang ◽  
Lizhi Fu ◽  
Lu Liu ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Antimicrobial Susceptibility ◽  
Streptococcus Suis

scholarly journals Antimicrobial Susceptibility, Multilocus Sequence Typing, and Virulence of Listeria Isolated From A Slaughterhouse

2021 ◽  
Author(s):  
Liting Wu ◽  
Hongduo Bao ◽  
Zhengquan Yang ◽  
Tao He ◽  
Yuan Tian ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Antimicrobial Susceptibility ◽  
Multilocus Sequence Typing ◽  
Tetracycline Resistance ◽  
Dilution Method ◽  
Processing Plant ◽  
Meat Processing ◽  
Processing Plants

Abstract Background: Listeria monocytogenes is one of the deadliest foodborne pathogens, and the bacterium can tolerate severe environments through biofilm formation and antimicrobial resistance. The objective of this study was to investigate the antimicrobial susceptibility, resistance genes,virulence and molecular epidemiology about Listeria from meat processing environments. Methods: This study evaluated the antibiotic resistance and virulence of Listeria isolates from slaughtering and processing plants. All isolates were subjected to antimicrobial susceptibility testing by using a standard microbroth dilution method. The carrying of resistant genes were identified by Polymerase Chain Reaction (PCR). The multilocus sequence typing (MLST) was determined subtyping of the isolates and to characterize possible routes of contamination from meat processing environments. The virulence of different STs of L. monocytogenes isolates were evaluated by Caco-2 cells invasion assay. Results: A total of 59 Listeria isolates were identified from 320 samples, including 37 L. monocytogenes (62.71%). This study evaluated the virulence of L. monocytogenes and antibiotic resistance of Listeria isolates from slaughtering and processing plants. The susceptibility of these 59 isolates against eight antibiotics was analyzed, and the resistance levels to ceftazidime, ciprofloxacin, and lincomycin were as high as 98.31% (L. m 37; L. innocua 7; L. welshimeri 14), 96.61% (L. m 36; L. innocua 7; L. welshimeri 14), and 93.22% (L. m 35; L. innocua 7; L. welshimeri 13) respectively. Over 90% of the isolates were resistant to 3-6 antibiotics, indicating that Listeria isolated from meat processing environments has high antimicrobial resistance. Up to 60% of the isolates carried the tetracycline-resistance genes tetA and tetM. The frequencies of ermA, ermB, ermC, and aac(6’)-Ib were 16.95%, 13.56%, 15.25%, and 6.78%, respectively. Notably, the resistant phenotype and genotype did not match exactly, suggesting that the mechanisms of antibiotic resistance of these isolates were likely related to the processing environment. Multilocus sequence typing (MLST) revealed that 59 Listeria isolates were grouped into 10 sequence types (STs). The dominant L. monocytogenes STs were ST5, ST9, and ST121 in the slaughtering and processing plant of Jiangsu province. Moreover, ST5 subtypes exhibited high invasion in Caco-2 cells compared with ST9 and ST121. Conclusions: The results of this study predict a prevalence of Listeria contamination in the slaughtering and processing plant , and resistance of the ST5 subtypes isolates to the antimicrobials may cause potential public health risks.

scholarly journals Prevalence of Multidrug-Resistant Enterococcus faecalis in Hospital-Acquired Surgical Wound Infections and Bacteremia: Concomitant Analysis of Antimicrobial Resistance Genes

2019 ◽  
Vol 12 ◽  
pp. 117863371988292 ◽  
Author(s):  
Mona Abdel Monem Esmail ◽  
Hend M Abdulghany ◽  
Rasha MM Khairy
Keyword(s):  
Enterococcus Faecalis ◽  
Resistance Genes ◽  
Antimicrobial Susceptibility ◽  
Antimicrobial Agents ◽  
Blood Cultures ◽  
Wound Infections ◽  
Surgical Wound ◽  
Erythromycin Resistance ◽  
Antimicrobial Resistance Genes ◽  
Hospital Acquired

Background: The study aimed to assess the prevalence of Enterococcus faecalis infections among patients with hospital-acquired surgical wound sepsis and bacteremia in surgical wards and identify the antimicrobial susceptibility in these pathogens. Genetic role of erythromycin, vancomycin, and cephalosporin resistance in these pathogens was also examined. Methods: Two hundred samples were collected from surgical wound infections and 100 blood cultures from patients with suggested bacteremia to identify E faecalis by phenotypic and genotypic methods. Antimicrobial susceptibility to 12 antimicrobial agents was tested. The presence of resistance genes was examined by polymerase chain reaction (PCR) assay. Results: E faecalis was isolated with a frequency of 24/200 (12%) from surgical wound samples and 2/100 (2%) from blood cultures. All isolates were completely resistant to cefepime, ampicillin, and tetracycline, 96% of isolates were resistant to erythromycin, 53.8% to vancomycin, and 23.1% to linezolid. Multidrug resistance (MDR) was found in 100% of isolates. ere(B) and erm(B) genes were present in 20/25 (80%) and 17/25 (68%) of erythromycin-resistant isolates, respectively, 15 (60%) isolates carry both ere(B) and erm(B) genes. Van A gene was detected in 71.4% of vancomycin-resistant isolates. All isolates were negative for mef(A/E), blaSHV, and blaTEM genes. Conclusion: MDR in all isolates (100%) and high-level resistance to gentamicin, erythromycin, and vancomycin were reported in E Faecalis isolates. In the studied isolates, erythromycin resistance mainly related to the presence of ere(B) and erm(B) genes and vancomycin resistance was mainly related to the presence of vanA gene.

scholarly journals Occurrence and Characterization of Salmonella Isolated from Large-Scale Breeder Farms in Shandong Province, China

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Jie Yang ◽  
Siwei Gao ◽  
Yajie Chang ◽  
Mingliu Su ◽  
Yutong Xie ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Antimicrobial Susceptibility ◽  
Multilocus Sequence Typing ◽  
Large Scale ◽  
Shandong Province ◽  
Sequence Type ◽  
Class 1 ◽  
Resistance Rates

This study aimed to investigate the prevalence and antimicrobial resistance of Salmonella spp. isolated from large-scale breeder farms in Shandong Province, China. A total of 63 Salmonella isolates (63/409, 15.4%) were identified from 409 samples collected from five large-scale breeder farms in Shandong Province. These Salmonella isolates were assayed for serotype, antimicrobial susceptibility, prevalence of class 1 integrons, quinolone resistance genes, and β-lactamase genes and subtyped by multilocus sequence typing (MLST). Among these isolates, S. Enteritidis (100%) was the predominant serovar, and high antimicrobial resistance rates to nalidixic acid (100.0%), streptomycin (100.0%), ampicillin (98.4%), and erythromycin (93.7%) were observed. All of the isolates carried blaTEM. MLST results showed that only one sequence type (ST11) was identified. Our findings indicated that Salmonella was generally prevalent not only on broiler farms but also on breeder farms.

scholarly journals Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Quan Li ◽  
Jian Yin ◽  
Zheng Li ◽  
Zewei Li ◽  
Yuanzhao Du ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Antimicrobial Susceptibility ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Resistance Rate ◽  
Economic Losses ◽  
Production Chain ◽  
Antimicrobial Resistance Genes

AbstractSalmonella is an important food-borne pathogen associated with public health and high economic losses. To investigate the prevalence and the characteristics of Salmonella in a pig slaughterhouse in Yangzhou, a total of 80 Salmonella isolates were isolated from 459 (17.43%) samples in 2016–2017. S. Derby (35/80, 43.75%) was the most prevalent, followed by S. Rissen (16/80, 20.00%) and S. Newlands (11/80, 13.75%). The highest rates of susceptibility were observed to cefoxitin (80/80, 100.0%) and amikacin (80/80, 100.0%), followed by aztreonam (79/80, 98.75%) and nitrofurantoin (79/80, 98.75%). The highest resistance rate was detected for tetracycline (65/80, 81.25%), followed by ampicillin (60/80, 75.00%), bactrim (55/80, 68.75%), and sulfisoxazole (54/80, 67.50%). Overall, 91.25% (73/80) of the isolates were resistant to at least one antibiotic, while 71.25% (57/80) of the isolate strains were multidrug resistant in the antimicrobial susceptibility tested. In addition, 86.36% (19/22) of the 22 antimicrobial resistance genes in the isolates were identified. Our data indicated that the resistance to certain antimicrobials was significantly associated, in part, with antimicrobial resistance genes. Furthermore, 81.25% (65/80) isolates harbored the virulence gene of mogA, of which 2 Salmonella Typhimurium isolates carried the mogA, spvB and spvC virulence genes at the same time. The results showed that swine products in the slaughterhouse were contaminated with multidrug resistant Salmonella commonly, especially some isolates carry the spv virulence genes. The virulence genes might facilitate the dissemination of the resistance genes to consumers along the production chain, suggesting the importance of controlling Salmonella during slaughter for public health.

Loss of erythromycin resistance genes from strains of Streptococcus pyogenes that have developed resistance to levofloxacin

2009 ◽  
Vol 64 (2) ◽  
pp. 225-228 ◽  
Author(s):  
Dewan Sakhawat Billal ◽  
Muneki Hotomi ◽  
Steve S. Yan ◽  
Daniel P. Fedorko ◽  
Jun Shimada ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Resistance Genes ◽  
Erythromycin Resistance

High Rates of Streptococcus Agalactiae Clindamycin and Erythromycin Resistance in Vietnamese Pregnant Women

2021 ◽  
Author(s):  
Vu Du ◽  
Pham Thai Dung ◽  
Nguyen Linh Toan ◽  
Can Mao ◽  
Nguyen Thanh Bac ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Prevention And Control ◽  
Control Strategies ◽  
Geographic Area ◽  
Resistance Rate ◽  
Antibiotics Resistance ◽  
Erythromycin Resistance ◽  
Antimicrobial Susceptibility Pattern ◽  
And Control

Abstract Background Group B streptococcus (GBS) is a leading cause of morbidity and mortality in newborns. Maternal GBS colonization rates vary depending on geographic area, ethnic and social conditions worldwide. Many studies suggested the continuous surveillance of GBS to provide data to guide decision-making and planning prevention and control strategies. Here, we report the rate and the antimicrobial susceptibility pattern of GBS from Vietnamese pregnant women over 5 year period. Methods We worked with 3863 Vietnamese pregnant women at < 37 weeks of gestation at the National Hospital of Obstetrics and Gynecology, Hanoi, Vietnam from Jan 2016 to Dec 2020. The data were recorded and retrieved from the computerized laboratory database. GBS was identified according to the American Society for Microbiology’s guidelines. Antimicrobial susceptibility was tested by the VITEK 2 system or E-test strips. The results were calculated according to the MIC breakpoints recommended by the Clinical and Laboratory Standards Institute. Results The positivity for GBS was 8.02% (310/3863) and the highest resistance rate was to tetracycline 89.66% (234/261), followed by 76.23% (202/265) for erythromycin, 58.21% (156/268) for clindamycin. The multidrug-resistance rate was 59.19% (161/272), and 8.46% (23/272) of isolates were resistant to 6 to 7 of the 12 antibiotics. Resistance to clindamycin in the absence of erythromycin resistance was found in 6/272 (2.2%) samples. The resistance rate to clindamycin was significantly increased (p < 0.01) over the time study period. Nevertheless, all isolates were sensitive to penicillin, ampicillin, ceftriaxone, cefotaxime, quinupristin/dalfopristin, and vancomycin. Conclusion Our results indicate that penicillin and ampicillin are currently the drugs of choice for the prevention and treatment of GBS-related diseases for Vietnamese pregnant women. However, antibiotic resistance to erythromycin and clindamycin was high. Thus, it reinforces the need for continuous surveillance of GBS to provide data to guide planning prevention and control strategies.

Antimicrobial Susceptibility and Characterization ofPropionibacterium acnesby Multilocus Sequence Typing and Repetitive-Sequence-Based PCR

2016 ◽  
Vol 46 (3) ◽  
pp. 135 ◽  
Author(s):  
Shukho Kim ◽  
Hyesoon Song ◽  
Weon-Ju Lee ◽  
Jungmin Kim
Keyword(s):  
Antimicrobial Susceptibility ◽  
Repetitive Sequence ◽  
Multilocus Sequence Typing

scholarly journals Non-Penicillin-Susceptible Streptococcus suis Isolated from Humans

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1178
Author(s):  
Nichari Bamphensin ◽  
Peechanika Chopjitt ◽  
Rujirat Hatrongjit ◽  
Parichart Boueroy ◽  
Nahuel Fittipaldi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Streptococcus Suis ◽  
Amino Acid Substitutions ◽  
Continuous Control ◽  
Antimicrobial Resistance Genes ◽  
Genes Encoding ◽  
Invasive Infections ◽  
Almost All

Streptococcus suis is a pathogen that causes invasive infections in humans and pigs. In this study, 448 S. suis isolates recovered from human infections in Thailand were characterized with regard to their antimicrobial susceptibility and antimicrobial resistance genes, including, for non-penicillin-susceptible isolates, sequence analyses of five genes encoding penicillin-binding proteins (pbp1a, pbp1b, pbp2a, pbp2b, and pbp2x). All 448 isolates were susceptible to cefepime and ceftriaxone, whereas 99.6%, 91.7%, and 72.9% of the isolates were susceptible to levofloxacin, penicillin, and chloramphenicol, respectively. Almost all isolates were resistant to tetracycline (98.2%), clindamycin (94%), erythromycin (92.4%), and azithromycin (82.6%). Genes tet(O) and ermB were the predominant resistance genes detected among macrolide- and tetracycline-resistant isolates. A total of 37 out of 448 isolates (8.2%) showed intermediately resistance to penicillin. Most of these isolates (59.5%) belonged to serotype 2-ST233. Comparison of the predicted translated sequences of five PBP proteins of a penicillin-susceptible isolate (strain P1/7) to the respective PBP sequences of ten non-penicillin-susceptible isolates revealed multiple amino acid substitutions. Isolates of CC221/234 showed highly variable amino acid substitutions in all PBP proteins. An ST104 isolate had a higher number of amino acid substitutions in PBP2X. Isolates belonging to CC233/379 had numerous substitutions in PBP2B and PBP2X. ST25 isolates exhibited fewer amino acid substitutions than isolates of other STs in all five PBPs. The antimicrobial resistance of S. suis is increasing worldwide; therefore, restrictions on antimicrobial use, continuous control, and the surveillance of this bacterium throughout the pork supply chain are crucial for ensuring public health and must be a priority concern.

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