scholarly journals Immunohistochemical analysis of the distribution of classical swine fever (CSF) viral antigen in boar-pig hybrids and pigs four weeks after infection

2021 ◽  
Author(s):  
Mayuko OKI ◽  
Mitsutaka IKEZAWA ◽  
Tatsuya NISHI ◽  
Katsuhiko FUKAI ◽  
Manabu YAMADA
Keyword(s):  
Viral Antigen ◽  
Classical Swine Fever ◽  
Immunohistochemical Analysis

scholarly journals Effects of classical swine fever virus infection on the porcine leukocyte subsets

2000 ◽  
Vol 48 (1) ◽  
pp. 35-42 ◽  
Author(s):  
H. J. Schubert ◽  
P. Soós ◽  
K. R. Depner
Keyword(s):  
Flow Cytometry ◽  
Virus Infection ◽  
Viral Antigen ◽  
Classical Swine Fever ◽  
Virus Antigen ◽  
Lower Percentage ◽  
Cd4 Cells ◽  
Double Positive ◽  
Cd8 Cells ◽  
Acute Form

The effects of classical swine fever (CSF) virus infection on the porcine leukocyte subsets were investigated by flow cytometry in acute, chronic and convalescent forms of the disease. The virus antigen could be first detected in the monocytes on postinfection (p.i.) day 10 while in the lymphocytes on p.i. day 13. It could be established that the ratio of CD6+ cells decreased until p.i. day 6, but afterwards it started to increase and reached different values. The CD4+CD8+, the CD8+ and the CD6- cells were obviously higher virus positive than the CD4+ and the CD4-CD8-subsets, but essentially all subsets could be infected. The ratio of CD8+ cells increased during the disease, while the number of double positive cells decreased, and that of the CD4+ cells was variable. The viral antigen could be detected in a lower percentage of the CD4+CD8+, CD8+, CD6+ and CD6- cells of the pigs affected with the chronic form of the disease than in those with the acute form. During the experiments no viral antigen could be detected in the leukocytes of the pig that became convalescent, though the changes in its leukocyte subsets were very similar to those seen in pigs in which the viral antigen could be detected. The studies have revealed that essentially all leukocyte subsets can be infected with the CSF virus, but in very different amounts.

scholarly journals CLASSICAL SWINE FEVER VIRUS DETECTION IN FETAL SWINE TISSUES BY IMMUNOHISTOCHEMISTRY

2020 ◽  
Vol 13 (1) ◽  
pp. 83-100
Author(s):  
Vladimir Polaček ◽  
Biljana Đurđević ◽  
Tamaš Petrović ◽  
Jasna Prodanov-Radulović ◽  
Milena Samojlović ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Endothelial Cells ◽  
Classical Swine Fever Virus ◽  
Viral Antigen ◽  
Ileocecal Valve ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Late Gestation ◽  
Fixed Tissue ◽  
Formalin Fixed

The classical swine fever virus has the ability to cross the placental barrier, resulting in the infection of fetuses, which may consequently lead to persistent infection in piglets. The aim of this study was to report the lesions in fetuses naturally infected with CSFV during late gestation and clarify the nature of infected cells and the distribution of viral antigen in different tissues. A total of twenty-nine fetuses aged 82, 83 and 95 gestational days originating from three naturally CSFV infected sows were examined in this study. In all tested sows and their fetuses CSFV was detected using RT-PCR method. Immunohistochemistry method was used to detect viral antigen and monoclonal antibody WH303 was used on formalin fixed tissue samples of brain, spleen, heart, tonsils, kidney, ileocecal valve and umbilical cord. The most common lesions in the majority of fetuses were hyperemia, petechial haemorrhages in the skin, lymph nodes and kidneys. With the exception of myocardium, CSF viral antigen was detected in all the examined tissues. WH303 positive cells included endothelial cells, monocytes, macrophages and lymphocytes. The largest number of positive cells was found in kidneys in all of the examined fetuses. Reticular cells, macrophages, lymphocytes and endothelial cells in the spleen were also intensely and widely stained in most of the fetuses. These results showed that CSFV antigen can be detected in formalin-fixed, paraffin-embedded fetal tissue specimens originating from naturally CSFV infected sows by using monoclonal antibody WH303. Fetal kidneys proved to be a very useful organ for diagnosis of the CSF virus. Having that in mind, it is assumed that persistently infected piglets may shed a high amount of viral particles through urine. However, further research is needed to confirm this hypothesis.

Classical Swine Fever: Pathology of Bone Marrow

2003 ◽  
Vol 40 (2) ◽  
pp. 157-163 ◽  
Author(s):  
J. C. Gómez-Villamandos ◽  
F. J. Salguero ◽  
E. Ruiz-Villamor ◽  
P. J. Sánchez-Cordón ◽  
M. J. Bautista ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Viral Infection ◽  
Viral Antigen ◽  
Classical Swine Fever ◽  
Morphometric Study ◽  
Immunohistochemical Detection ◽  
Middle Phase ◽  
Virulent Isolate ◽  
Quantitative Changes ◽  
Qualitative Changes

Twenty pigs were inoculated with a virulent isolate (Quillota strain) of classical swine fever (CSF) virus to determine the chronological development of lesions in bone marrow. Histopathologic, ultrastructural and immunohistochemical (detection of viral antigen gp55, myeloid-histiocyte antigen, CD3 antigen, and FVIII-rag), and morphometric techniques were employed. Viral antigen was detected from 2 days postinfection (dpi) in stromal and haematopoitic cells, and severe atrophy related to apoptosis of haematopoitic cells was observed. Megakaryocytes (MKs) did not show significant changes in number, but there were important qualitative changes including 1) increased numbers of cloud-nuclei MKs, microMKs, apoptotic MKs, and atypical nucleated MKs and 2) decreased number of typical nucleated MKs. Morphometric study of these cells showed a decrease in cytoplasmic area. MK infection was detected from 2 dpi, but in a small percentage of cells. Myeloid cells showed quantitative changes, with an increase in granulocyte numbers. Apoptosis of lymphocytes and viral infection of erythroblasts were also observed. The main changes in stroma were depletion of T lymphocytes in the middle phase of the experiment and macrophages. Viral infection was also observed in these cells. MK lesions suggest dysmegakaryocytopoiesis, which would aggravate the thrombocytopenia already present and could be responsible for it. Granulocyte changes would lead to the appearance of circulating immature forms, whereas lymphocyte apoptosis in bone marrow would contribute to lymphopenia.

scholarly journals Viral gene expression during acute simian varicella virus infection

2002 ◽  
Vol 83 (4) ◽  
pp. 841-846 ◽  
Author(s):  
Wayne L. Gray ◽  
Lisa Mullis ◽  
Kenneth F. Soike
Keyword(s):  
Gene Expression ◽  
Viral Antigen ◽  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Pcr Analysis ◽  
Viral Gene ◽  
Vervet Monkeys ◽  
Simian Varicella Virus ◽  
Varicella Zoster ◽  
Varicella Virus

Simian varicella virus (SVV) causes a natural varicella-like disease in nonhuman primates. Outbreaks of simian varicella occur sporadically in primate facilities. Simian varicella is used as a model for investigation of varicella-zoster virus (VZV) pathogenesis and latency. In this study, SVV gene expression and histopathology were analysed in tissues of acutely infected vervet monkeys. RT–PCR analysis demonstrated expression of specific SVV immediate early, early and late genes in the skin, lung, liver and ganglia tissues of acutely infected monkeys. Viral antigen expression and histopathology, including necrosis and inflammation, were detected in the skin, lungs, liver and spleen of infected monkeys by immunohistochemical analysis. Viral antigen expression, but little or no histopathology, was evident in the neural ganglia, the eventual site of viral latency. The study provides a foundation for further investigation on the role of viral genes in varicella pathogenesis and latency.

Germinal centre localization of bovine viral diarrhoea virus in persistently infected animals

Microbiology ◽  
2000 ◽  
Vol 81 (7) ◽  
pp. 1669-1673 ◽  
Author(s):  
M. D. Fray ◽  
E. A. Supple ◽  
W. I. Morrison ◽  
B. Charleston
Keyword(s):  
B Cell ◽  
Viral Genome ◽  
Viral Antigen ◽  
Bovine Viral Diarrhoea Virus ◽  
Immunohistochemical Analysis ◽  
Bovine Viral Diarrhoea ◽  
Persistently Infected ◽  
Germinal Centres ◽  
Peripheral Lymph ◽  
Diarrhoea Virus

Immunohistochemical analysis of peripheral lymph nodes from gnotobiotic calves persistently infected with bovine viral diarrhoea virus (BVDV) revealed extensive deposition of Erns and localization of the viral genome in the light zone of germinal centres. Viral antigen co-localized with immunoglobulin in the germinal centres and was shown to be extracellular. Despite the presence of viral antigen in germinal centres, circulating anti-BVDV antibody was not detected. These findings provide evidence that calves persistently infected with BVDV, in the absence of adventitious infection, can generate a B cell response to the persisting virus. The nature of the tolerance in calves persistently infected with BVDV is discussed in light of these findings.

995: Immunohistochemical Analysis of Tumor Polyamines Discriminates High Risk Patients Undergoing Nephrectomy for Renal Cell Carcinoma

2004 ◽  
Vol 171 (4S) ◽  
pp. 263-263
Author(s):  
Nathalie Rioux-Leclercq ◽  
Florence Jouan ◽  
Pascale Bellaud ◽  
Jacques-Philippe Moulinoux ◽  
Karim Bensalah ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
High Risk ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Immunohistochemical Analysis ◽  
High Risk Patients ◽  
Risk Patients

Immunohistochemical analysis of CD138-positive Plasma cells in the endometrium

2016 ◽  
Vol 76 (05) ◽  
Author(s):  
M Weber ◽  
B Toth ◽  
E Schleußner ◽  
UR Markert
Keyword(s):  
Plasma Cells ◽  
Immunohistochemical Analysis

Expression of Platelet Membrane Glycoprotein V in Human Megakaryocytes and Megakaryocytic Cell Lines: A Study Using a Novel Monoclonal Antibody against GPV

1994 ◽  
Vol 72 (05) ◽  
pp. 762-769 ◽  
Author(s):  
Toshiro Takafuta ◽  
Kingo Fujirmura ◽  
Hironori Kawano ◽  
Masaaki Noda ◽  
Tetsuro Fujimoto ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Lines ◽  
Immunohistochemical Analysis ◽  
Specific Protein ◽  
Platelet Membrane ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Flow Cytometric ◽  
Polymerase Chain ◽  
Megakaryocytic Cell

SummaryGlycoprotein V (GPV) is a platelet membrane protein with a molecular weight of 82 kD, and one of the leucine rich glycoproteins (LRG). By reverse transcription-polymerase chain reaction (RT-PCR), GPV cDNA was amplified from mRNA of platelets and megakaryocytic cell lines. However, since there are few reports indicating whether GPV protein is expressed in megakaryocytes as a lineage and maturation specific protein, we studied the GPV expression at the protein level by using a novel monoclonal antibody (1D9) recognizing GPV. Flow cytometric and immunohistochemical analysis indicated that GPV was detected on the surface and in the cytoplasm of only the megakaryocytes in bone marrow aspirates. In a megakaryocytic cell line UT-7, GPV antigen increased after treatment with phorbol-12-myri-state-13-acetate (PMA). These data indicate that only megakaryocytes specifically express the GPV protein among hematopoietic cells and that the expression of GPV increases with differentiation of the megakaryocyte as GPIb-IX complex.

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