scholarly journals Significance of Fecal Volatile Fatty Acids in Shedding of Escherichia coli O157 from Calves: Experimental Infection and Preliminary Use of a Probiotic Product.

2000 ◽  
Vol 62 (11) ◽  
pp. 1151-1155 ◽  
Author(s):  
Tatsuo OHYA ◽  
Toshihiko MARUBASHI ◽  
Hiroya ITO
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Experimental Infection ◽  
Volatile Fatty Acids ◽  
Escherichia Coli O157 ◽  
Probiotic Product

Factors influencing the persistence ofEscherichia coliO157:H7 lineages in feces from cattle fed grain versus grass hay diets

2010 ◽  
Vol 56 (8) ◽  
pp. 667-675 ◽  
Author(s):  
Ross M.S. Lowe ◽  
Krysty Munns ◽  
L. Brent Selinger ◽  
Linda Kremenik ◽  
Danica Baines ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Hemorrhagic Colitis ◽  
Escherichia Coli O157 ◽  
Negative Bacterium ◽  
Gram Negative ◽  
E Coli ◽  
Factors Influencing ◽  
Uremic Syndrome

Escherichia coli O157:H7 is a pathogenic, gram-negative bacterium that causes diarrhea, hemorrhagic colitis, and can lead to fatal hemolytic uremic syndrome in humans. We examined the persistence of E. coli O157:H7 lineages I and II in feces held at 4, 12, and 25 °C, from animals fed either grain or hay diets. Three strains of each lineage I and II were inoculated into grain-fed or hay-fed feces, and their persistence was monitored over 28 days. No significant differences in E. coli O157:H7 survival between the 2 lineages in both fecal types was found at the examined temperatures. Volatile fatty acids were higher in grain-fed than in hay-fed feces, resulting in consistently lower pH in the grain-fed feces at 4, 12 and 25 °C. Regardless of lineage type, E. coli O157:H7 CFUs were significantly higher in grain-fed than in hay-fed feces at 4 and 25 °C. Escherichia coli O157:H7 survival was highest in grain-fed feces at 25 °C up to 14 days. Our results indicate that the 2 lineages of E. coli O157:H7 do not differ in their persistence; however, it appears that temperature and feces type both affect the survival of the pathogen.

scholarly journals Utilization of14C-labelledBacillus subtilisandEscherichia coliby sheep

1970 ◽  
Vol 24 (1) ◽  
pp. 129-144 ◽  
Author(s):  
N. J. Hoogenraad ◽  
F. J. R. Hird ◽  
R. G. White ◽  
R. A. Leng
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
Fatty Acids ◽  
Cell Walls ◽  
Volatile Fatty Acids ◽  
The Body ◽  
Air Plasma ◽  
Whole Cells ◽  
Total Glucose ◽  
Bacterial Preparations

1.Bacillus subtilisandEscherichia coliwere grown on14C-labelled glucose and used for the preparation of labelled whole cells, cell walls, cell contents and peptidoglycan.2. The radioactive samples were injected into the abomasum of sheep and the14C appearing in expired air, plasma glucose, urine and faeces was determined. Whole cells were also injected into the rumen and the incorporation of14C into volatile fatty acids was measured.3. All the bacterial preparations, including cell walls, were extensively digested and absorbed, Less than 15% of the radioactivity was recovered in the faeces.4. Up to 20% of the radioactivity injected was recovered in expired carbon dioxide with only 2.4–8.1% passing through the glucose pool.5. It has been calculated that under the conditions of the experiment 18.5 % of the total glucose entering the body pool of glucose in 24 h was derived from bacterial carbon.

scholarly journals Survival of Escherichia coli O157:H7 in Soils from Vegetable Fields with Different Cultivation Patterns

2013 ◽  
Vol 79 (5) ◽  
pp. 1755-1756 ◽  
Author(s):  
Zhiyuan Yao ◽  
Gang Wei ◽  
Haizhen Wang ◽  
Laosheng Wu ◽  
Jianjun Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Organic Carbon ◽  
Open Field ◽  
Soil Ph ◽  
Phospholipid Fatty Acids ◽  
Escherichia Coli O157 ◽  
Field Soil ◽  
Content Type ◽  
Greenhouse Cultivation

ABSTRACTEscherichia coliO157:H7 survived longer in soils from plastic-greenhouse cultivation than soils from the open field. Soil pH, organic carbon levels, and the ratio of bacterial phospholipid fatty acids (PLFAs) to fungal PLFAs played the significant roles in survival of O157:H7. Greater attention should be paid to the control of pathogen contamination under conditions of plastic-greenhouse cultivation.

Survival of Escherichia coli O157:H7 in ruminal or fecal contents incubated with corn or wheat dried distillers’ grains with solubles

2010 ◽  
Vol 56 (11) ◽  
pp. 890-895 ◽  
Author(s):  
H.E. Yang ◽  
W.Z. Yang ◽  
J.J. McKinnon ◽  
T.W. Alexander ◽  
Y.L. Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Volatile Fatty Acids ◽  
Barley Grain ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Distillers Grains With Solubles ◽  
The Media ◽  
Distillers Grain ◽  
Dried Distillers Grain ◽  
Corn Ddgs

Dried distillers’ grain with solubles (DDGS) is a by-product of ethanol production, and its use as cattle feed has increased as a result of the expansion of the fuel ethanol industry. However, the inclusion of corn DDGS into feedlot diets may increase the shedding of Escherichia coli O157:H7. This study investigated whether corn or wheat DDGS at 2 concentrations (20% or 40% vs. 100% barley grain) affected the survival of E. coli O157:H7 in incubations of ruminal digesta and feces. Neither the type nor the level of DDGS had any effect on fermentation or the survival of E. coli O157:H7 in ruminal digesta. However, there was a time by DDGS interaction (p < 0.05), where the numbers of E. coli O157:H7 in feces did not differ after 4 or 12 h of incubation but were greater after 24 h in both 40% wheat and 40% corn DDGS as compared with other treatments. Additionally, after 24 h, the numbers of E. coli O157:H7 were greater in fecal incubations with corn DDGS than with wheat DDGS (p < 0.05). The differences in the numbers of E. coli O157:H7 were not attributable to changes in pH or in concentrations of volatile fatty acids in the media. These results suggest that the inclusion of high levels of corn or wheat DDGS in feedlot diets of cattle may encourage the survival of E. coli O157:H7 in feces.

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