scholarly journals ISOLASI SENYAWA AKTIF DAN UJI AKTIVITAS EKSTRAK JAHE MERAH (ZINGIBER OFFICINALE) SEBAGAI IMUNOMODULATOR

2021 ◽  
Vol 3 (2) ◽  
pp. 77-91
Author(s):  
Masniah Masniah ◽  
Jafril Rezi ◽  
Ahmad Purnawarman Faisal
Keyword(s):  
Lymphocyte Proliferation ◽  
Ethanol Extract ◽  
Zingiber Officinale ◽  
Immunomodulatory Activity ◽  
Ginger Extract ◽  
Immunostimulatory Activity ◽  
Macrophage Cells ◽  
Macrophage Phagocytosis ◽  
Red Ginger

Red ginger (Zingiber officinale) contains many chemical compounds that have many activities. This study aims to determine the active compounds of Zingiber officinale and immunomodulatory activity that can increase the phagocytosis of macrophage cells and lymphocyte cell proliferation. Red ginger extract compounds were identified using GC-MS. The parameters of immunostimulatory activity were macrophage phagocytosis and lymphocyte proliferation, the preparations were made in several extract concentrations. This research is an experimental study in the laboratory. The research included fresh red ginger, made into ethanol extract, dried. The results were made a suspension, the extract was then given to macrophage cells (phagocytosis testing) and lymphocyte cells (proliferation testing) in vitro to test the immunomodulatory effect. The results showed that the most active extract in macrophage phagocytosis activity was the extract concentration of 500 ppm. While the activity of lymphocyte proliferation was shown by the concentration of group A (Cell, 500 ppm extract, MK, Engerik). The active compound contained in the ethanol extract of red ginger is thought to be Geraniol

scholarly journals Aggressiveness and Libido of Male Mus musculus L. in Ethanol Extract of Red Ginger (Zingiber officinale Roxb. var. Rubrum)

2019 ◽  
Vol 6 (1) ◽  
pp. 8-14
Author(s):  
Nida Nurhanifah ◽  
Sutyarso Sutyarso ◽  
M. Kanedi ◽  
Hendri Busman
Keyword(s):  
Free Radicals ◽  
Ethanol Extract ◽  
Serum Testosterone ◽  
Zingiber Officinale ◽  
The Body ◽  
Male Mice ◽  
Ginger Extract ◽  
Treatment Groups ◽  
Randomized Design ◽  
Red Ginger

The residue of paraquat herbicides may cause human health problems. Paraquat causes the testosterone hormone to decline. Testosterone hormone affects aggressive and sexual behavior. It is caused by free radicals in the body. Free radicals can be non-reactive if in the body there are antioxidants. Red ginger could be used as an antioxidant because it has active phenolic compounds, such as arginine, gingerol, shogaol, zingerone, gingerdiol, and zingiber. Ginger could increase serum testosterone levels. The purpose of this research is to know the effect of red ginger ethanol extract on aggressiveness and libido of male mice induced by paraquat herbicide. This study used a Completely Randomized Design (RAL) consisting of six treatment groups with four replications each. Group P1 as control (aqua dest), P2 (paraquat at dose 20 mg/kg BM), P3, P4, P5 (paraquat with dose 20 mg/kg BM and red ginger extract at 200 mg/kg BM, 400 mg/kg BM, 600 mg/kg BM) and P6 (red ginger extract at 600mg/kg BM). Paraquat is given 2 times a week for 21 days and the red ginger extract is given daily for 35 days. The result of analysis with One-way ANOVA and continued BNT at 5% showed that giving of red ginger ethanol extract can increase aggressive behavior and libido of male mice induced paraquat herbicide.

scholarly journals UJI AKTIVITAS FAGOSITOSIS MAKROFAG EKSTRAK ETANOL DAUN SUJI (Dracaena angustifolia (Medik.)Roxb. ) SECARA IN VITRO

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Nestri Handayani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Immunomodulatory Activity ◽  
Macrophage Activity ◽  
Macrophage Phagocytosis ◽  
Phagocytosis Index ◽  
Layer Chromatography

UJI AKTIVITAS FAGOSITOSIS  MAKROFAG  EKSTRAK ETANOL  DAUN SUJI (Dracaena angustifolia (Medik.)Roxb. ) SECARA  IN VITRONestri Handayani 1,2*), Subagus Wahyuono2), Triana Hertiani2), Retno Murwanti2)1)Program Studi Farmasi, FMIPA, Universitas Sebelas Maret Surakarta2)Fakultas Farmasi Universitas Gadjah Mada Yogyakarta*Email : [email protected]  ABSTRACT Immunomodulatory activity has been carried out on ethanol extract of suji leaf (Dracaena angustifolia (Medik.) Roxb with in vitro macrofag phagocytosis method. . Suji is a plant that is often used as a food coloring and has long been used medicinally. This study aimed to test in vitro macrophage phagocytosis on ethanol extract of suji leaf. The suji leaves are extracted by maceration method. The obtained viscous extract was then tested for Thin Layer Chromatography (TLC) and in vitro macrophage phagocytosis test. The parameters used are Phagocytosis Index and Macrophage Capacity. The results of in vitro macrophage phagocytosis test with 4 concentrations of 10.25,50 and 100 microgram / ml, showed for the largest average Fagocytosis (IF) Index shown at concentration 10 mcg / ml, while concentrations 25 and 50 mcg / ml showed values The same phagocytic (KF) capacity. Keywords :  Suji leaf, Phagocytic Macrophage Activity,  ethanolic  extract   ABSTRAKUji aktivitas imunomodulator telah dilakukan terhadap ekstrak etanol  daun suji (Dracaena angustifolia (Medik.)Roxb..  Suji merupakan tanaman yang sering digunakan sebagai pewarna makanan dan telah lama digunakan untuk obat. Penelitian ini bertujuan untuk melakukan uji fagositosis makrofag in vitro terhadap ekstrak etanol daun suji. Daun suji diekstraksi dengan metode maserasi. Ekstrak kental yang diperoleh kemudian dilakukan uji Kromatografi Lapis Tipis (KLT) dan uji fagositosis makrofag in vitro. Parameter yang digunakan adalah Indeks fagositosis dan Kapasitas makrofag. Hasil uji fagositosis makrofag in vitro dengan 4 konsentrasi sebesar 10,25,50 dan 100 mikrogram/ml, menunjukkan untuk Indeks Fagositosis (IF) rata-rata terbesar ditunjukkan pada konsentrasi 10 mcg/ml, sedangkan konsentrasi 25 dan 50 mcg/ml menunjukkan nilai  Kapasitas Fagositosis (KF) yang sama besar. Kata kunci : Daun suji, fagositosis makrofag, ekstrak etanol    

scholarly journals Organ-Protective Effect of Red Ginger Extracts in Male Mice Induced by Paraquat

2019 ◽  
Vol 6 (2) ◽  
pp. 8-17
Author(s):  
Kamiliah Tsany ◽  
Sutyarso Sutyarso ◽  
M. Kanedi ◽  
Hendri Busman
Keyword(s):  
Ethanol Extract ◽  
Zingiber Officinale ◽  
The Body ◽  
Control Group ◽  
Male Mice ◽  
Ginger Extract ◽  
Randomized Design ◽  
Significant Difference ◽  
Lung Histology ◽  
Red Ginger

Paraquat is a toxic compound that disrupts biochemical processes in the body, cell death, and multi-organ failure. The liver and lung are the target organ of paraquat toxicity. The body needs antioxidants to counteract free radicals. Red ginger (Zingiber officinale Roxb. Var rubrum) is a spice that contains antioxidants. This study was conducted to determine the effect of ethanol extract of red ginger (Zingiber officinale Roxb. Var rubrum) on liver and lung histology male mice induced by paraquat. The research used completely randomized design and use of 6 treatment groups, i.e. control group (given distilled water and standard feed), PQ group (induced by paraquat 20 mg/kg per BW), group P1 (given paraquat and ethanol extracts of red ginger 200 mg/kg per BW), group P2 (given paraquat and ethanol extract of red ginger 400 mg/kg per BW), group P3 (given paraquat and ethanol extract of red ginger 600 mg/kg per BW), group P4 (given ethanol extract of red ginger extract 600 mg/kg per BW). The data obtained will be tested using One Way ANOVA and continued by Least Significant Difference (LSD) with a 95% confidence level. The results showed ethanol extract of red ginger effective against liver and lung histology male mice induced by paraquat. The results also showed ethanol extract of red ginger with a dose of 400 mg/kg per BW is effective in protecting the liver and lung from damage caused induced by paraquat.

scholarly journals Antibacterial Activity Test of Red Ginger Extract (Zingiber officinale var. Rubrum) Against Streptococcus pyogenes In vitro

2020 ◽  
Vol 3 (1) ◽  
pp. 24
Author(s):  
Samira Assegaf ◽  
Arthur Pohan Kawilarang ◽  
Retno Handajani
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Bacterial Suspension ◽  
Ginger Extract ◽  
Mueller Hinton ◽  
Red Ginger ◽  
Mueller Hinton Broth

Introduction: Red ginger (Zingiber officinale var. rubrum) is known as an antibacterial agent. Streptococcus pyogenes is the most common bacterial cause of pharyngitis in children and adults. Over the years, antibiotic resistance case is increasing. Therefore, further research of other substances to find an bactericidal or bacteriostatic agent is needed. The aim of this study is to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Z. officinale var. rubrum against S. pyogenes. Methods: Serial dilution test of red ginger extract in Mueller hinton broth (8 concentrations of red ginger extract : 80%, 40%, 20%, 10%, 5%, 2,5%, 1,25%, and 0,625%) and positive control (1 ml bacterial suspension in Mueller hinton broth) was used to determine MIC. MBC was determined by culturing solutions from the previous dilution test into blood agar plate.lResults: MIC of red ginger extract against S. pyogenes could not be determined because the color of red ginger extract affected the turbidity of the dilution test result. MBC of red ginger extract against S. pyogenes was at concentration 20% of red ginger extract.Conclusion: Z. officinale var. rubrum showed antibacterial activity against S. pyogenes.

scholarly journals Influence of Zingiber officinale Extract on Push-Out Bond Strength of Glass-Fiber Post

2018 ◽  
Vol 29 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Tânia Mara da Silva ◽  
Virgílio Vilas Boas Fernandes Junior ◽  
Rosemary Soares de Santana ◽  
Renata Marques de Melo Marinho ◽  
Márcia Carneiro Valera ◽  
...  
Keyword(s):  
Bond Strength ◽  
Glass Fiber ◽  
Zingiber Officinale ◽  
Resin Cement ◽  
Fiber Post ◽  
Ginger Extract ◽  
Etch And Rinse ◽  
Glass Fiber Post ◽  
Push Out

Abstract The aim of this study was to evaluate in vitro the influence of 20% glycolic ginger extract on the bond strength of glass-fiber post cemented with etch-and-rinse or self-etching resin cement. Forty-eight bovine roots were standardized (17±0.5 mm) and randomly divided into two groups, according to irrigant used during biomechanical preparation: NaOCl: 1% sodium hypochlorite and GEN: 20% glycolic ginger extract. Root canal was prepared and randomly assigned to one of two subgroups (n=12), according to luting protocol: self-etching (RelyX U200) and etch-and-rinse (RelyX ARC). After 48 h, the roots were sectioned perpendicularly (4 in cervical third and 3 in middle third) and submitted to push-out bond strength test (50 kgf load cell, 1.0 mm/min). Failure mode was analyzed in SEM and stereomicroscopy. Data were analyzed by three-way ANOVA and Tukey tests (p<0.05). ANOVA revealed that there were no significant differences between U200 (2.01±0.17)B and ARC (1.93±0.12)B in GEN group, and at the cervical third for both irrigants, 2.69±0.16A and 2.54±0.17A, respectively. Middle third showed lower bond strength than cervical third, regardless the irrigant. Overall, the cervical third regardless the irrigant employed and the association between NaOCl and ARC, presented better adhesive performance.

OPTIMASI FORMULA EKSTRAK JAHE MERAH (Zingiber officinale) DENGAN METODE KEMPA LANGSUNG MENGGUNAKAN ANALISIS SIMPLEX LATTICE DESIGN

2017 ◽  
Vol 1 (1) ◽  
pp. 47
Author(s):  
Hayatus Sa'adah
Keyword(s):  
Dosage Form ◽  
Ethanol Extract ◽  
Zingiber Officinale ◽  
Disintegration Time ◽  
Lattice Design ◽  
Simplex Lattice Design ◽  
Optimum Proportion ◽  
Simplex Lattice ◽  
Red Ginger ◽  
Tablet Hardness

Processing of medicinal plants into the appropriate dosage form can ensure security during use. It is a motivation in making acceptable dosage form which is easy and convenient to use, especially the manufacture of ethanol extract red ginger tablets using a combination of starch 1500 and amprotab.The study begins with the manufacture of dry extract of red ginger. Optimization of making tablets using a combination of starch 1500 and disintegrator with simplex lattice design using three formulas is done by direct compaction method. Further testing on the tablet hardness, friability and disintegration time.The results showed starch 1500 has a greater influence increase hardness and disintegration time of tablets, as well as lowering the fragility of the tablet. While the interaction of starch 1500 and disintegrator has no effect on the physical tablet. The optimum proportion of the combination of starch 1500 and disintegrator meet the physical requirements of tablets with a ratio of 4: 6 with 7.99 kg hardness, the friability of 0.32% and disintegration time of 2.42 minutes

scholarly journals Immunomodulatory Activity and Phytochemical Profile of Infusions from Cleavers Herb

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3721 ◽  
Author(s):  
Tetiana Ilina ◽  
Weronika Skowrońska ◽  
Natalia Kashpur ◽  
Sebastian Granica ◽  
Agnieszka Bazylko ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Herbal Remedy ◽  
Folk Medicine ◽  
Immunomodulatory Activity ◽  
Blast Transformation ◽  
Immunostimulatory Activity ◽  
Traditional Use ◽  
Aerial Parts ◽  
Lymphocyte Blast Transformation

Extracts from aerial parts of G. aparine (cleavers) constitute a herbal remedy with monography in British Herbal Pharmacopeia. On the European market, there are several drugs and food supplements consisting of Galium extracts. In folk medicine, cleavers was used topically in Europe, Asia, and the Americas to treat skin diseases. In several remedies, cleavers is also listed as an immunomodulatory active herb influencing the defense response of the human body. The aim of this study was to investigate the immunostimulatory activity and antioxidant potential in vitro of a raw infusion of cleavers and bioactive fractions. The functional activity of lymphocytes in the reaction of the lymphocyte blast transformation (RLBT) method was used for immunomodulatory activity assays and direct scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), and hydrogen peroxide (H2O2) was chosen for the examination of antioxidant activity. It was shown that both the raw extract and fractions show significant immunostimulatory and scavenging activities. The obtained data partially justify the traditional use of cleavers as topical remedy for skin infections and for wounds.

scholarly journals WS-5 Extract of Curcuma longa, Chaenomeles sinensis, and Zingiber officinale Contains Anti-AChE Compounds and Improves β-Amyloid-Induced Memory Impairment in Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-16 ◽  
Author(s):  
Ju Eun Kim ◽  
Abinash Chandra Shrestha ◽  
Hyo Shin Kim ◽  
Ha Neul Ham ◽  
Jun Hyeong Kim ◽  
...  
Keyword(s):  
Memory Impairment ◽  
Antioxidant Activities ◽  
Curcuma Longa ◽  
Senile Plaques ◽  
Total Antioxidant Status ◽  
Ethanol Extract ◽  
Zingiber Officinale ◽  
Chaenomeles Sinensis

Alzheimer's disease (AD) is linked to an extensive neuron loss via accumulation of amyloid-beta (Aβ) as senile plaques associated with reactive astrocytes and microglial activation in the brain. The objective of this study was to assess the therapeutic effect of WS-5 ethanol extract in vitro and in vivo against Aβ-induced AD in mice and to identify the extract’s active constituents. In the present study, WS-5 exerted a significant inhibitory effect on acetylcholinesterase (AChE). Analysis by transmission electron microscopy (TEM) revealed that WS-5 prevented Aβ oligomerization via inhibition of Aβ1-42 aggregation. Evaluation of antioxidant activities using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) demonstrated that WS-5 possessed a high antioxidant activity, which was confirmed by measuring the total antioxidant status (TAS). Furthermore, the anti-inflammatory properties of WS-5 were examined using lipopolysaccharide-stimulated BV-2 microglial cells. WS-5 significantly inhibited the lipopolysaccharide–induced production of nitric oxide and two proinflammatory cytokines, TNF-α and IL-6. The memory impairment in mice with Aβ-induced AD was studied using the Morris water maze and passive avoidance test. Immunohistochemistry was performed to monitor pathological changes in the hippocampus and cortex region of the mouse brain. The animal study showed that WS-5 (250 mg/kg) treatment improved learning and suppressed memory impairment as well as reduced Aβ plaque accumulation in Aβ-induced AD. HPLC analysis identified the extract’s active compounds that exert anti-AChE activity. In summary, our findings suggest that WS-5 could be applied as a natural product therapy with a focus on neuroinflammation-related neurodegenerative disorders.

scholarly journals IMMUNOMODULATORY EFFECTS OF ETHANOL EXTRACT OF CURCUMA MANGGA RHIZOMES IN MICE

2017 ◽  
Vol 10 (9) ◽  
pp. 148 ◽  
Author(s):  
Yuandani Yuandani ◽  
Edy Suwarso
Keyword(s):  
Therapeutic Potential ◽  
Ethanol Extract ◽  
Negative Control ◽  
Total Leukocyte Count ◽  
Phagocytic Index ◽  
Immunomodulatory Activity ◽  
Immunomodulatory Effects ◽  
Immunostimulatory Activity ◽  
Immunomodulatory Potential

Objective: This study was conducted to evaluate the immunomodulatory effects of ethanol extract of Curcuma mangga by in vivo study.Methods: The ethanol extract of C. mangga was comprised to carbon clearance method for its immunomodulatory potential. The extract wasadministered orally at doses of 100, 200, and 400 mg/kg BW to mice for 7 days. On day 8, carbon ink was injected, and the blood was collected formeasurement of elimination of carbon. Total leukocyte count was also determined.Results: The evaluation of immunomodulatory potential of ethanol extract of C. mangga revealed a dose-dependent increase in phagocytosis ability.The phagocytic index of ethanol extract of C. mangga was more than those of negative control, indicating the immunostimulatory activity of C. mangga.It showed low stimulation on total leukocyte count.Conclusion: The results indicate that ethanol extract of C. mangga rhizomes possesses immunomodulatory activity and has therapeutic potential forthe treatment of infectious diseases.

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