scholarly journals Sterile Tissue Preparation and Callus Induction of Curcuma longa Linn.

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
Warunya Kaewthip ◽  
Srisulak Dheeranupattana ◽  
Pornchai Junta ◽  
Lalida Shank
Keyword(s):  
Callus Induction ◽  
Agar Medium ◽  
Curcuma Longa ◽  
Multiple Shoots ◽  
Leaf Sheath ◽  
Naphthaleneacetic Acid ◽  
Tissue Preparation ◽  
Friable Callus ◽  
Sterile Leaf ◽  
Dichlorophenoxy Acetic Acid

Curcuma longa Linn. (family Zingiberaceae), commonly known as ‘turmeric’, is native to Southeast Asia. Turmeric has been used for color, flavor as a spice in cuisine and employed for treatment of various diseases. The major component in yellow-pigmented fraction of turmeric is curcuminoids. Curcuminoid production in callus of C. longa Linn. is our focus of study. Sterile techniques to obtain germ-free of C. longa Linn. explants were investigated and the results showed that immersing rhizome buds in 70% ethanol for 5 min, followed by 0.10% HgCl2 for 10 min offered approximately 66% survival rate. Multiple shoots were generated from the aseptic rhizome explants cultured on Murashige and Skoog (MS) agar medium fortified with 3.00 µM of 6-Benzylaminopurine (BA) and 0.50 µM of 1-Naphthaleneacetic acid (NAA) at 25 ± 2°C under a photoperiod of 16 h light and 8 h dark. The sterile leaf sheath and root were subsequently used for callus induction which produced various responses when cultured on MS agar medium fortified with different concentrations of 2,4-dichlorophenoxy acetic acid (2, 4-D), Thidiazuron (TDZ) and BA. The highest induction yields of friable callus were obtained from leaf sheath segments cultured on MS agar medium fortified with 0.50 mg/l 2, 4-D which are the conditions proposed for successful production of callus culture of C. longa Linn. Keywords: Callus induction, Curcuma longa Linn., Turmeric, Plant tissue culture

scholarly journals Micropropagation, Callus Induction and Regeneration of Ginger (Zingiber officinale Rosc.)

2020 ◽  
Vol 5 (1) ◽  
pp. 75-84
Author(s):  
Seied Mehdi Miri
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Shoot Number ◽  
Micro Propagation ◽  
Dichlorophenoxy Acetic Acid

AbstractThe present study describes a protocol for micro-propagation, callus induction, and shoot regeneration of ginger (Zingiber officinale). The rhizomes were surface-sterilized with ethanol (70%) for 45 s, sodium hypochlorite (2.5%) for 10 min, and mercuric chloride (0.1%) for 10 min. Multiple shoots were induced from sprouting bud explants cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) combined with kinetin (Kin). The maximum shoot number was obtained from MS medium containing 10 mg/l BA with a mean of 20.6 shoots per explant. The leaf explants were cultured on MS medium supplemented with indole-3-acetic acid (IAA), naphthaleneacetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), Dicamba, or BA for callus culture. Green-red compact calli were induced using 2,4-D, Dicamba or BA. Also, BA successfully induced plant regeneration. The multiplied shoots that were transferred to the rooting medium (½MS supplemented with 0, 1 and 2 mg/l IAA, indole-3-butyric acid (IBA) or NAA) showed development of roots (100%). The rooted plantlets were transferred to pots containing a 1:1 mixture of cocopeat and perlite, and acclimatization was successful, resulting in 85% survival of the plantlets in the greenhouse.

High-Frequency Shoot Regeneration From Flower Bud Derived Callus of Gymnostachyum Febrifugum Benth., an Endemic Medicinal Plant to the Western Ghats

2021 ◽  
Author(s):  
Silpa P ◽  
Dennis Thuruthiyil Thomas
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Shoot Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Flower Bud ◽  
The Family ◽  
Endemic Medicinal Plant ◽  
Dichlorophenoxy Acetic Acid ◽  
The Western Ghats

Abstract Gymnostachyum febrifugum Benth. is a small, scapigerous, rare and endemic medicinal herb indigenous to India belonging to the family Acanthaceae. This study reports an efficient protocol for high-frequency flower bud derived callus induction and shoot organogenesis in G. febrifugum. Flower buds at 7d before anthesis (dBA) were excised from the inflorescence and cultured on MS medium supplemented with various concentrations of 2, 4-dichlorophenoxy acetic acid (2, 4-D; 0.5-2.0 mg/l) for callus induction. The optimum callus induction (78%) was obtained on MS medium supplemented with 1.5 mg/l 2, 4-D. The calli when subcultured on MS medium supplemented with different concentrations of thidiazuron (TDZ; 0.5-2.5 mg/l) or 6-benzylaminopurine BAP (0.5-2.5 mg/l) alone or in combination with 1- naphthaleneacetic acid (NAA; 0.2-0.7 mg/l) induced shoots. The highest frequency (94%) and number of shoots (44.6 shoots/unit callus) were obtained on MS medium supplemented with 2.0 mg/l TDZ and 0.5 mg/l NAA. The optimum rooting frequency (95%) and number of roots (10.2) were observed on ½ MS medium supplemented with 3.0 mg/l indole-3- butyric acid (IBA). The rooted plantlets were acclimatized and transferred to soil with 94% success.

scholarly journals Induction and Analysis of the Alkaloid Mitragynine Content of aMitragyna speciosaSuspension Culture System upon Elicitation and Precursor Feeding

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Nor Nahazima Mohamad Zuldin ◽  
Ikram Md. Said ◽  
Normah Mohd Noor ◽  
Zamri Zainal ◽  
Chew Jin Kiat ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Yeast Extract ◽  
Callus Induction ◽  
Cell Suspension Cultures ◽  
Naphthaleneacetic Acid ◽  
Precursor Feeding ◽  
Petiole Explants ◽  
Mitragyna Speciosa ◽  
Dichlorophenoxy Acetic Acid

This study aimed to determine the effects of different concentrations and combinations of the phytohormones 2,4-dichlorophenoxy acetic acid (2,4-D), kinetin, 6-benzylaminopurine (BAP), and 1-naphthaleneacetic acid (NAA) on callus induction and to demonstrate the role of elicitors and exogenous precursors on the production of mitragynine in aMitragyna speciosasuspension culture. The best callus induction was achieved from petiole explants cultured on WPM that was supplemented with 4 mg L−12, 4-D (70.83%). Calli were transferred to liquid media and agitated on rotary shakers to establishMitragyna speciosacell suspension cultures. The optimum settled cell volume was achieved in the presence of WPM that contained 3 mg L−12,4-D and 3% sucrose (9.47±0.4667 mL). The treatment of cultures with different concentrations of yeast extract and salicylic acid for different inoculation periods revealed that the highest mitragynine content as determined by HPLC was achieved from the culture treated with 250 mg L−1yeast extract (9.275±0.082 mg L−1) that was harvested on day 6 of culturing; salicylic acid showed low mitragynine content in all concentrations used. Tryptophan and loganin were used as exogenous precursors; the highest level of mitragynine production was achieved in cultures treated with 3 μM tryptophan and harvested at 6 days (13.226±1.98 mg L−1).

Plant Regeneration from Excised Cotyledons of Mature Strawberry Achenes

HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 569-571 ◽  
Author(s):  
A. Raymond Miller ◽  
Craig K. Chandler
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Multiple Shoot ◽  
Naphthaleneacetic Acid ◽  
Developmental Studies ◽  
Shoot Buds ◽  
Cotyledon Explants ◽  
Multiple Shoot Buds ◽  
Rapid Regeneration ◽  
Dichlorophenoxy Acetic Acid

A protocol was developed for excising and culturing cotyledon explants from mature achenes of strawberry (Fragaria × ananassa Duch.). Cotyledon explants formed callus with multiple shoot buds on agar-solidified Murashige and Skoog media containing several combinations of hormones (1 μm 2,4-D; 10 μm 2,4-D; 1 μm BA + 1 μm 2,4-D; 1 μm BA + 10 μm 2,4-D; 5 μm BA; 5 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μ m 2,4-D; 5 μ m BA + 5 μm NAA; 5 μ m BA + 15 μ m NAA). After three subcultures, only tissues maintained on the medium containing 5 μm BA + 5 μm NAA continued to form shoots. Tissues transferred to other media eventually died (1 μm 2,4-D; 1 μ m BA + 10 μ m 2,4-D; 5 μ m BA; 5 μ m BA + 1 μ m 2,4-D), became unorganized (1 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μm 2,4-D; 5 μm BA + 15 μm NAA), or formed roots (10 μm 2,4-D). Whole plantlets were produced by transferring callus with buds to medium lacking hormones. The rapid regeneration of clonal plantlets from cotyledon explants may be useful for reducing variability in future developmental studies. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); (2,4-dichlorophenoxy) acetic acid (2,4-D); and 1-naphthaleneacetic acid (NAA).

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

2015 ◽  
Vol 44 ◽  
pp. 38-44 ◽  
Author(s):  
H. Sandhya ◽  
Rao Srinath
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Basal Medium ◽  
Direct Regeneration ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Dichlorophenoxy Acetic Acid ◽  
Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

scholarly journals Effects of Plant Growth Regulators and Sugars on Ehretia asperula Zoll. et Mor. Cell Cultures

2021 ◽  
Author(s):  
P.T.M. Tram ◽  
N.K. Suong ◽  
L.T.T. Tien
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Malignant Tumors ◽  
Single Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Human Immune System ◽  
Friable Callus ◽  
2,4 Dichlorophenoxyacetic Acid

Background: Belonging to the Boraginacae family, Ehretia asperula Zoll. et Mor., called “Xa den”, is a precious medicinal plant also known as the “cancer tree” by the Muong ethnic group in Hoa Binh Province, Vietnam. Xa den has been demonstrated to inhibit the development of malignant tumors, reduce oxidation and enhance the human immune system. This research focused on examining friable callus induction from young stems of Ehretia asperula Zoll. et Mor. Methods: Samples of Xa den were less than two years old. Young stems with 2 to 6 leaves served as explants for callus induction. Explants placed on autoclaved B5 nutrients incubated at 25oC, in the dark. The testing factors were concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Benzyl adenine (BA), types and concentrations of sugars.Result: Friable callus was induced on B5 medium with 0.4 mg/L of 2,4-D, 0.1 mg/L of BA and 30 g/L of glucose at the highest rate (100%). Additionally, callus grew best after 5 weeks of culture weighing 0.194 g. Friable callus was used as material for cell suspension cultures. After two weeks, the Xa den cell suspension cultures contained single cells and small cell clumps. The liquid medium had changed from dark yellow to light brown.

scholarly journals Organogenesis Kelapa Sawit (Elaeis guineensis Jacq.) Asal Eksplan Bunga Betina

2016 ◽  
Vol 2 (2) ◽  
pp. 104
Author(s):  
Tyas Larasati ◽  
Suci Rahayu ◽  
Fauziyah Harahap
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Callus Induction ◽  
Plant Growth Regulator ◽  
Elaeis Guineensis ◽  
Female Flower ◽  
Dry Weight ◽  
Flower Plant ◽  
Elaeis Guineensis Jacq ◽  
Dichlorophenoxy Acetic Acid

The objectives of this research were to composed organ from callus culture and to found the best concentration of plant growth regulator for organ growth from female flower explant of oil palm. This research has already done from June 2014 to May 2015 at Laboratory of Plant Physiology and Tissue Culture Department of Biology Faculty of Mathematics and Science University of North Sumatera. This research used Nonfactorial Completely Random Design. Explant was treated with five concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D; 99, 110, 120, 132, and 140 mg/L) for callus induction on Y3 medium (Eeuwens 1976). The result of this research showed that organ was formed from this treatment (basal segment of female flower explant) was root organ. 2,4-D plant growth regulator positively affected to growing of the root. The best result for time of callus induction, time of root growth, the highest percentage of explants that formed the root, fresh weight and dry weight of callus that has become the root generation was resulted from 99 mg/L 2,4-D.   Key words: Elaeis guineensis Jacq., female flower, plant growth regulator 2,4-D, organogenesis

scholarly journals Etude Des Effets Des Régulateurs De Croissance Sur La Les Stades De Prolifération Et De Développement De La Pomme De Terre (Solanum Tuberosum. L) In Vitro

2017 ◽  
Vol 13 (24) ◽  
pp. 145
Author(s):  
Sadek Chahredine ◽  
Nadia Ykhlef
Keyword(s):  
Solanum Tuberosum ◽  
Callus Induction ◽  
Solanum Tuberosum L ◽  
Naphthaleneacetic Acid ◽  
Compact Structure ◽  
Potato Plants ◽  
Régulateurs De Croissance ◽  
Role Of Light

The aim of this study is to determine the effects of different concentrations and combinations of the phytohormones, 1-naphthaleneacetic acid (NAA), and 6-benzylaminopurine (BAP): M1 (0.5 mg / l +1 mg / l), M2 (1 mg / l + 0.5 mg / l) , M3 (2 mg / l +2 mg / l), M4 (0.5 mg / l + l mg / l, NAA), M5 (1.0 mg / l + l mg / l , NAA), and M6 (2.0 mg / l + l mg / l, NAA). This study was carried out in dark condition on callus induction of potato plants (Solanum tuberosum L.) cultivars from potato tuber bud so as to demonstrate the role of light. The callus initiation begins after 7 days of incubation for all studied media. After two months of incubation, the better development of callus was noted in Spunta variety by using medium M1, M2, M3, and M6. The calluses took a compact structure of brown-white color for both varieties with a callus induction rate of 20- 40%. This was collected with kondor variety for M2 and (M3, M4, M5) media respectively and 10-30% for M4 (M1, M2, M3) for Spunta variety also. The highest fresh weight was recorded on M2 medium with 0.26g for Kondor variety and 0.93g for Spunta variety.

scholarly journals In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

2010 ◽  
Vol 6 ◽  
pp. 103-105 ◽  
Author(s):  
Aditi Singh ◽  
Saroj K Sah ◽  
Aunji Pradhan ◽  
Sabari Rajbahak ◽  
Niran Maharajan
Keyword(s):  
Medicinal Plant ◽  
Callus Induction ◽  
Shoot Growth ◽  
Tinospora Cordifolia ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

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