scholarly journals In-vitro Anti-proliferative Effects of Ethanolic Extract of Vanilla Planifolia Leaf Extract against A431 Human Epidermoid Carcinoma Cells

2019 ◽  
Vol 12 (3) ◽  
pp. 1141-1146 ◽  
Author(s):  
Vijaybabu K ◽  
Punnagai K2
Keyword(s):  
Skin Cancer ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Carcinoma Cells ◽  
Dependent Manner ◽  
A431 Cells ◽  
Cancer Suppression ◽  
Dose Dependent ◽  
Human Epidermoid Carcinoma

Squamous cell carcinoma is the second largest among skin cancer diseases. The aim of the present study is to reveal the antiproliferative property of vanilla leaf extract against A431 cells. Antiproliferative property was assessed by MTT assay. Apoptotic property was assessed by DNA fragmentation assay. Antiproliferative property of extract was revealed in a dose dependent manner. IC50 of the extract against A431 cells was revealed at 31.2µg/ml. This study revealed the cancer suppression capability of vanilla leaf extract in skin cancer cell lines.

scholarly journals In-Vitro Evaluation of Anti-Urolithiatic and Larvicidal Activity of Alternanthera sessilis

2021 ◽  
Vol 14 (02) ◽  
pp. 671-680
Author(s):  
Merin Babu ◽  
Uma K.H ◽  
Sherin Joseph ◽  
Amoolya Sree ◽  
Sabin Scariya ◽  
...  
Keyword(s):  
Larvicidal Activity ◽  
Ethanolic Extract ◽  
Objective Evaluation ◽  
Assay Method ◽  
Dependent Manner ◽  
Alternanthera Sessilis ◽  
Larvicidal Effect ◽  
Whole Plant ◽  
Dose Dependent

Objective: Evaluation of Anti-urolithiatic and Larvicidal activity of Alternanthera sessilis. Method: The whole plant of Alternanthera sessilis were extracted using ethanol as solvent. Then it was evaluated for its phytochemicals and later on in vitro anti-urolithiatic study was conducted on the plant using the methods titrimetry, simultaneous flow static model, turbidimetry and gravimetric. The plant showing larvicidal effect was determined by larvicidal assay method. Result: The ethanolic extract of the plant showed the presence of various phytochemicals like phenols, flavonoids, tannins, sterols, saponins. The anti urolithiatic activity conformed that the plant can effectively mineralise calcium oxalate in a dose dependent manner when compared to control and standard. The plant also possesses larvicidal activity and the percentage mortality exhibited a dose dependent manner. Conclusion: The ethanolic extract of the plant possessed anti- urolithiatic as well as larvicidal activity.

scholarly journals Acaricidal activity of Furcraea foetida leaf extract against engorged female Rhipicephalus (Boophilus) microplus ticks

2021 ◽  
Vol 37 ◽  
pp. e37031
Author(s):  
Henrique Aparecido de Sousa Martins ◽  
Maria de Fatima Pereira ◽  
Enéas Ricardo Konzen ◽  
Gilvano Ebling Brondani ◽  
Wellington Ferreira Campos
Keyword(s):  
Crude Extract ◽  
Leaf Extract ◽  
Skin Lesions ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Economic Damage ◽  
Attractive Alternative ◽  
Dependent Manner ◽  
Dose Dependent

The Rhipicephalus (Boophilus) microplus tick is a major concern for the livestock market worldwide, as it causes serious economic damage. Plant-derived acaricides are an attractive alternative to control this ectoparasite and limit the development of resistance. Therefore, the aim of this study was to evaluate the acaricidal activity of Furcraea foetida leaf extract against engorged female R. (B.) microplus ticks. Our in vitro bioassays showed that the crude extract of leaves from F. foetida caused hemorrhagic swelling and skin lesions in the ticks, and three days of treatment caused 100% mortality. Dose-response assay indicated that this toxicity effect was dose-dependent. Similar effects were observed when the crude extract from F. foetida leaves was denatured by boiling at 100°C. These results suggest that the toxicity of the leaf extract might be associated with thermostable biomolecules. Together, our results show for the first time that the crude extract of F. foetida leaves has acaricidal activity against engorged female R. (B.) microplus ticks and it acts in a dose-dependent manner.

scholarly journals Ethanolic extract of ocimum kilimandscharicum linnleaves: phytochemical and in vitro pharmacological activity

2021 ◽  
pp. 106-109
Author(s):  
Yamini N ◽  
Lahari S ◽  
Phani deepthi V
Keyword(s):  
In Vitro Model ◽  
Ethanolic Extract ◽  
Clinical Development ◽  
Dependent Manner ◽  
Thrombolytic Activity ◽  
Standard Drug ◽  
Ethanolic Extracts ◽  
Vitro Model ◽  
Dose Dependent

Using an in vitro model, the anti-thrombolytic efficacy of ethanolic extracts of Ocimum kilimandscharicum Linn was investigated. The researchers discovered that different concentrations of the extract had significant anti-thrombolytic activity in a dose-dependent manner , which was comparable to a standard drug. As a result of the presence of flavonoids and polyphenols in the plant extract, it can be concluded that it has a promising future in the treatment of thrombosis. This knowledge will be useful in the clinical development of thrombolytic therapeutics by identifying more potent anti-thrombolytic principles from natural resources..    

scholarly journals EFFICACY OF AN ACTIVE COMPOUND OF THE HERB, ASHWAGANDHA IN PREVENTION OF STRESS INDUCED HYPERGLYCEMIA

2018 ◽  
Vol 10 (10) ◽  
pp. 44 ◽  
Author(s):  
Sarjan H. N. ◽  
Yajurvedi H. N.
Keyword(s):  
Phosphoenolpyruvate Carboxykinase ◽  
Ethanolic Extract ◽  
Dependent Manner ◽  
Stress Exposure ◽  
In Vitro Experiment ◽  
Isolated Compound ◽  
Dose Dependent ◽  
Different Doses

Objective: To find out whether an isolated compound (IC) from the ethanolic extract of roots of ashwagandha prevents stress-induced hyperglycemia by direct interference with the action of increased concentration of corticosterone on hepatocytes or by preventing hyper-secretion of corticosterone or both.Methods: A group of rats served as controls, and those in another group were subjected to restraint (1 h) and forced swimming exercise (15 min), after a gap of 4 h daily for 4 w. The third group of rats received orally IC (5 mg/kg bw/rat) 1 h prior to exposure to stressors. After the last treatment period, a blood sample was collected and serum was separated for the estimation of corticosterone and glucose. In in vitro experiment, hepatocytes were treated with different concentrations of corticosterone (100, 200, 300, 400 and 500 ng/ml). In another set of experiment, hepatocytes were treated with different doses of IC (1, 10, 100, 1000 and 10 000 μg/ml of medium) along with corticosterone (400ng/ml). The concentration of glucose and activities of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) were determined after the treatment.Results: Stress exposure caused a significant increase in serum concentration of corticosterone and glucose whereas, administration of IC did not result in similar changes. Further, treatment of corticosterone in in vitro significantly increased the activities of PEPCK and G6Pase and concentration of glucose in a dose-dependent manner in hepatocytes. However, treatment with IC did not interfere with the corticosterone-induced an increase in the activities of PEPCK and G6Pase as well as the concentration of glucose in hepatocytes.Conclusion: The in vivo and in vitro results put together reveal that IC does not directly interfere with the action of corticosterone on hepatocytes. However, it prevents stress-induced hyperglycemia by suppressing hyper-secretion of corticosterone. 

Pharmacognostic Profile, In-vitro Antioxidant and Hepatoprotective Potential of Ethanolic Fruit Extract of Pyrus communis L.

2021 ◽  
Vol 17 ◽  
Author(s):  
Sonia Singh ◽  
Meenakshi Bajpai ◽  
Pradeep Mishra
Keyword(s):  
Antiradical Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Pyrus Communis ◽  
Dependent Manner ◽  
Cell Protection ◽  
Standard Drug ◽  
Pyrus Communis L ◽  
Dose Dependent

Background: The ethanolic extract of Pyrus communis L. fruit (EEPC) was assessed for hepatoprotective and in vitro antiradical activity against carbon tetrachloride-induced hepatotoxicity in rat’s liver. Methods: The degree of hepatoprotection was screened by measuring biochemical parameters including serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total albumin (TA), total protein (TP) and total bilirubin (TB). The antiradical activity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide and nitric oxide free radical scavenging property. The hepatoprotective effects of the extract were compared with Silymarin used as a standard drug (100 mg/kg, p.o, bodyweight). Results: The ethanolic extract of the fruit has the capabilities to scavenge the free radicals, in vitro respectively. Additionally, the ethanolic extract (200 mg/kg and 400 mg/kg, p.o, bodyweight) exhibited marked hepatoprotective action in respect of CCl4 intoxicated rodents in a dose-dependent manner. EEPC at a dose of 400mg/kg could afford significant dose-dependent protection against CCl4 induced hepatocellular injury. Conclusion: Biochemical samples obtained from the animals treated with ethanolic extracts (400mg/kg, body weight) showed a significant decrease in the levels of serum markers indicating the hepatic cell protection.

scholarly journals ERK1/2 and HIF1αAre Involved in Antiangiogenic Effect of Polyphenols-Enriched Fraction from Chilean Propolis

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Alejandro Cuevas ◽  
Nicolás Saavedra ◽  
Martina Rudnicki ◽  
Dulcineia S. P. Abdalla ◽  
Luis A. Salazar
Keyword(s):  
Endothelial Cells ◽  
Western Blot ◽  
Mrna Expression ◽  
Ethanolic Extract ◽  
Tube Formation ◽  
Dependent Manner ◽  
Antiangiogenic Effect ◽  
Dose Dependent

Propolis has been shown to modulate the angiogenesis in bothin vitroandin vivomodels. Thus, we aimed to evaluate the antiangiogenic properties of an ethanolic extract of Chilean propolis (EEP) and Pinocembrin (Pn). Migration, formation of capillary-like structures of endothelial cells, and sprouting from rat aortic rings were used to assess the antiangiogenic properties of EEP or Pn. In addition, microRNAs and VEGFA mRNA expression were studied by qPCR. ERK1/2 phosphorylation and HIF1αstabilization were assessed by western blot. EEP or Pn attenuated the migration, the capillary-like tube formation, and the sprouting in thein vitroassays. In addition, the activation of HIF1αand ERK1/2 and the VEGFA mRNA expression was significantly inhibited in a dose-dependent manner. In summary, these results suggest that HIF1αand ERK1/2 phosphorylation could be involved in the antiangiogenic effect of Chilean propolis, but more studies are needed to corroborate these findings.

scholarly journals Amaranth leaf extract protects against hydrogen peroxide induced oxidative stress in Drosophila melanogaster

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ndinawe Johnmark ◽  
Hellen W. Kinyi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Drosophila Melanogaster ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Dependent Manner ◽  
Polyphenol Compounds ◽  
Dose Dependent

Abstract Objective Amaranths leaves are rich in ascorbic acid and polyphenol compounds which have antioxidant activity. The aim of this study was to evaluate their in vivo antioxidant activity. The effect of consumption of Amaranth leaf extract on in vivo antioxidant activity, catalase enzyme activity and H2O2 induced oxidative stress in Drosophila melanogaster flies was assessed. Results Consumption of Amaranth leaf extract was associated with increased survival on exposure to H2o2 in a dose dependent manner in Drosophila melanogaster flies. The study concludes that the ethanolic extract of Amaranth leaves offer protection against hydrogen peroxide-induced oxidative stress.

IN VITRO ANTI-INFLAMMATORY AND ANTI-ARTHRITIC ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF PYRENACANTHA VOLUBILIS (EEPV)

2020 ◽  
pp. 156-158
Author(s):  
ANJALI P ◽  
VIMALAVATHINI R ◽  
KAVIMANI S
Keyword(s):  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Dependent Manner ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Human Red Blood Cells ◽  
Anti Inflammatory ◽  
Dose Dependent

Objectives: The study was undertaken to evaluate the in vitro anti-inflammatory and anti-arthritic activity of the ethanolic extract of leaves of Pyrenacantha volubilis (EEPV) using human red blood cells (HRBCs) membrane stabilization and protein denaturation methods. Methods: In the present study, the in vitro anti-inflammatory and anti-arthritic activity of EEPV was carried out using HRBC membrane stabilization by hypotonicity-induced hemolysis and protein denaturation using egg albumin methods at various concentrations (100, 200, 400, 800, and 1000) of EEPV. Diclofenac sodium was used as reference standard. Results: P. volubilis was effective in inhibiting HRBC membrane stabilization and protein denaturation in a dose-dependent manner and was comparable to the standard drug diclofenac sodium. Conclusion: The study suggests that P. volubilis has potential anti-inflammatory and anti-arthritic activity.

scholarly journals Continentalic Acid Rather Than Kaurenoic Acid Is Responsible for the Anti-Arthritic Activity of Manchurian Spikenard In Vitro and In Vivo

2019 ◽  
Vol 20 (21) ◽  
pp. 5488
Author(s):  
Riwon Hong ◽  
Kyoung Soo Kim ◽  
Gwang Muk Choi ◽  
Mijung Yeom ◽  
Bombi Lee ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Dependent Manner ◽  
Amino Terminal ◽  
Kaurenoic Acid ◽  
Mitogen Activated Protein ◽  
Continentalic Acid ◽  
Pg E2 ◽  
Dose Dependent

The aim of this study was to identify the active compound responsible for the pharmacological activities of Manchurian spikenard (Aralia continentalis Kitag.). Interleukin (IL)-1β-stimulated human chondrocytes and monoiodoacetate (MIA)-induced osteoarthritic rats were treated with the 50% ethanolic extract of spikenard or its major components, such as continentalic acid (ent-pimara-8(14),15-diene-19-oic acid) and kaurenoic acid (ent-kaura-16-en-19-oic acid). The spikenard extract significantly inhibited IL-1β-stimulated production of IL-6, IL-8, metalloproteinase (MMP)-1, MMP-13, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) and prostaglandin(PG)E2 in a dose-dependent manner but not MMP-3 production. The extract also inhibited the IL-1β-induced translocation of NF-κB/p65 into the nucleus and dose-dependent phosphorylation levels of extracellular signal-regulated kinase (ERK), Jun amino-terminal kinase (JNK) and p38 mitogen-activated protein (MAP) kinase. Continentalic acid exhibited significant anti-arthritic activity corresponding exactly to that of the extract containing an equivalent amount of continentalic acid. On the other hand, kaurenoic acid exhibited a compatible activity at about a 10-times higher molar concentration than that of continentalic acid. In vitro anti-arthritic activities of the spikenard extract and continentalic acid were also confirmed in MIA-induced osteoarthritic rats. The 50% ethanolic extract of Manchurian spikenard exhibited promising anti-arthritic activities in the in vitro and in vivo osteoarthritis models, and continentalic acid, not kaurenoic acid, was most probably responsible for those activities.

scholarly journals In vitro Cercaricidal Activity of Fractions and Isolated Compounds of Erythrophleum ivorense (Fabaceae) Root Bark against Schistosoma haematobium

2019 ◽  
pp. 1-9
Author(s):  
Francis A. Armah ◽  
Benjamin Amoani ◽  
Isaac T. Henneh ◽  
Rita A. Dickson ◽  
Christian K. Adokoh ◽  
...  
Keyword(s):  
Schistosoma Haematobium ◽  
Public Health Problem ◽  
Ethanolic Extract ◽  
Bioactive Compound ◽  
Major Public Health Problem ◽  
Root Bark ◽  
Dependent Manner ◽  
Immature Form ◽  
Dose Dependent

Introduction: Schistosoma haematobium is one of the species of Schistosoma responsible for schistosomiasis in humans, a major public health problem worldwide. Praziquantel, the most effective drug against all adult stages of human schistosomiasis, faces the threat of resistance and also has sub-optimal efficacy against cercaria, an immature form of schistosomiasis. This underscores the need to search for an alternative anti-schistosomal drug with pronounced activity particularly against cercaria. Aim: This study investigated anti-cercarial activity of total crude (70% ethanolic extract), fractions (methanolic, ethyl acetate and petroleum ether) and isolated bioactive compounds from the root bark of Erythrophleum ivorense. Study Design: In vitro anti-cercarial activity was evaluated using 20 freshly shed cercariae from Schistosoma haematobium species transferred into 20 well plates. Cercaricidal effect of the various concentrations (15.6, 31.3, 62.5, 125.0, 250.0 and 500.0 µg/mL) of test extracts and compounds were observed for 3 hours using an inverted microscopy. The results showed that extracts and compounds of the plant decreased percentage viability of cercariae in a dose-dependent manner. Results: Within two hours of incubation, all cercariae died at the various concentrations of test compounds and extracts with the exception of methanol extract and the bioactive compound erythroivorensin at 15.6

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