scholarly journals Bioethanol from Guinea Grass (Panicum Maximum) and Ragi Tempeh as Fermentation Starter using Bioreactor and Shaker

2019 ◽  
Vol 35 (4) ◽  
pp. 1302-1312
Author(s):  
Y. C. Wong ◽  
Z. K. Lam
Keyword(s):  
Accurate Result ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Panicum Maximum ◽  
Acid Pretreatment ◽  
Lignocellulosic Feedstock ◽  
Guinea Grass ◽  
Freezing Test ◽  
Test Result ◽  
Fermentation Starter

This study was investigating the production of bioethanol from the mixture of guinea grass (Panicum maximum) and using ragi tempeh through fermentation. The acid pretreatment process was carried out using 15% v/v sulphuric acid. The fermentation was carried out in anaerobic condition using bioreactor and shaker at 37°C and different pH of 5,6,7, and 8. Freezing test and Tollens’ test result showed very ethanol concentration in the sample product is very low, and aldehyde compounds are present in the product sample. The aldehyde is released due to degradation of lignocellulosic feedstock by acid hydrolysis. HPLC is carried out for getting a more accurate result. The best result is chromatogram of sample 5, obtained by fermentation using shaker with pH 8. It shows normal chromatogram with ethanol yield of 7.89%.

scholarly journals Evaluation of Copper-Contaminated Marginal Land for the Cultivation of Vetiver Grass (Chrysopogon zizanioides) as a Lignocellulosic Feedstock and its Impact on Downstream Bioethanol Production

2019 ◽  
Vol 9 (13) ◽  
pp. 2685 ◽  
Author(s):  
Emily M. Geiger ◽  
Dibyendu Sarkar ◽  
Rupali Datta
Keyword(s):  
Contaminated Soil ◽  
Ethanol Yield ◽  
Dilute Acid Pretreatment ◽  
Cellulose Content ◽  
Bioethanol Production ◽  
Vetiver Grass ◽  
Acid Pretreatment ◽  
Biofuel Feedstock ◽  
Lignocellulosic Feedstock ◽  
The Impact

Metal-contaminated soil could be sustainably used for biofuel feedstock production if the harvested biomass is amenable to bioethanol production. A 60-day greenhouse experiment was performed to evaluate (1) the potential of vetiver grass to phytostabilize soil contaminated with copper (Cu), and (2) the impact of Cu exposure on its lignocellulosic composition and downstream bioethanol production. Dilute acid pretreatment, enzymatic hydrolysis, and fermentation parameters were optimized sequentially for vetiver grass using response surface methodology (RSM). Results indicate that the lignocellulosic composition of vetiver grown on Cu-rich soil was favorably altered with a significant decrease in lignin and increase in hemicellulose and cellulose content. Hydrolysates produced from Cu exposed biomass achieved a significantly greater ethanol yield and volumetric productivity compared to those of the control biomass. Upon pretreatment, the hemicellulosic hydrolysate showed an increase in total sugars per liter by 204.7% of the predicted yield. After fermentation, 110% of the predicted ethanol yield was obtained for the vetiver grown on Cu-contaminated soil. By contrast, for vetiver grown on uncontaminated soil a 62.3% of theoretical ethanol yield was achieved, indicating that vetiver has the potential to serve the dual purpose of phytoremediation and biofuel feedstock generation on contaminated sites.

scholarly journals Sustainable 2G Ethanol Production From Switchgrass Biomass via Co-Fermentation of Pentoses and Hexoses Using Novel Wild Yeasts

2021 ◽  
Author(s):  
Felipe A. F. Antunes ◽  
Kalavathy Rajan ◽  
Angele Djioleu ◽  
Thiago M. Rocha ◽  
Larissa P. Brumano ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Ethanol Yield ◽  
Enzymatic Saccharification ◽  
Dilute Acid Pretreatment ◽  
Acid Pretreatment ◽  
Lignocellulosic Feedstock ◽  
Lignocellulosic Feedstocks ◽  
Wild Yeasts ◽  
Alkaline Delignification ◽  
Maximum Utilization

Abstract The production of second generation (2G) ethanol remains an interesting proposition for the implementation of sustainable and net carbon-neutral energy systems. 2G makes use of renewable lignocellulosic feedstocks, generating fermentable sugars that are converted to ethanol or other bio-based products. To be economically viable, 2G biorefineries must make use of all processing streams, including the less desirable C5 sugar stream. In this work, a strategy of sequential acid and alkaline pretreatment of the lignocellulosic feedstock switchgrass for improvement of fermentable sugar yield, and the subsequent utilization of wild yeasts for co-fermentation of its C5-C6 sugar streams are presented. Hemicellulose-enriched hydrolysates, obtained by dilute acid pretreatment of switchgrass, were fermented by a newly-isolated wild Scheffersomyces parashehatae strain–UFMG-HM-60.1b; corresponding ethanol yield (YPS) and volumetric productivity (QP) were 0.19 g/g and 0.16 g/L h, respectively. Afterwards, the remaining switchgrass cellulignin fraction was subjected to optimized alkaline delignification at 152 ºC for 30 min. The delignified solid fraction was subjected to contiguous enzymatic saccharification and fermentation, releasing a C6 sugar stream in which Saccharomyces cerevisiae 174 strain displayed a productivity of 0.46 g/g (YPS) and 0.70 g/L h (QP), whereas the S. parashehatae UFMG-HM-60.1b presented YPS and QP of 0.29 g/g and 0.38 g/L h, respectively. Upon combining the conversion of hemicellulose (37%) and cellulose-derived sugars (57%), the S. parashehatae strain provided higher yield (94%) than the generic S. cerevisiae (90%). Henceforth, our integrated pretreatment and co-fermentation process provides a pathway for maximum utilization of the switchgrass carbohydrates for 2G ethanol production.

scholarly journals Hemicellulosic Bioethanol Production from Fast-Growing Paulownia Biomass

Processes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 173
Author(s):  
Elena Domínguez ◽  
Pablo G. del Río ◽  
Aloia Romaní ◽  
Gil Garrote ◽  
Lucília Domingues
Keyword(s):  
Ethanol Production ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Xylose Reductase ◽  
Energy Crop ◽  
Scheffersomyces Stipitis ◽  
Fractionation Process ◽  
Renewable Source ◽  
Fast Growing ◽  
Engineered Strain

In order to exploit a fast-growing Paulownia hardwood as an energy crop, a xylose-enriched hydrolysate was obtained in this work to increase the ethanol concentration using the hemicellulosic fraction, besides the already widely studied cellulosic fraction. For that, Paulownia elongata x fortunei was submitted to autohydrolysis treatment (210 °C or S0 of 4.08) for the xylan solubilization, mainly as xylooligosaccharides. Afterwards, sequential stages of acid hydrolysis, concentration, and detoxification were evaluated to obtain fermentable sugars. Thus, detoxified and non-detoxified hydrolysates (diluted or not) were fermented for ethanol production using a natural xylose-consuming yeast, Scheffersomyces stipitis CECT 1922, and an industrial Saccharomyces cerevisiae MEC1133 strain, metabolic engineered strain with the xylose reductase/xylitol dehydrogenase pathway. Results from fermentation assays showed that the engineered S. cerevisiae strain produced up to 14.2 g/L of ethanol (corresponding to 0.33 g/g of ethanol yield) using the non-detoxified hydrolysate. Nevertheless, the yeast S. stipitis reached similar values of ethanol, but only in the detoxified hydrolysate. Hence, the fermentation data prove the suitability and robustness of the engineered strain to ferment non-detoxified liquor, and the appropriateness of detoxification of liquor for the use of less robust yeast. In addition, the success of hemicellulose-to-ethanol production obtained in this work shows the Paulownia biomass as a suitable renewable source for ethanol production following a suitable fractionation process within a biorefinery approach.

scholarly journals Transcriptomic data of pre-meiotic stage of floret development in apomictic and sexual types of guinea grass (Panicum maximum Jacq.)

Data in Brief ◽  
2018 ◽  
Vol 18 ◽  
pp. 590-593 ◽  
Author(s):  
Auji Radhakrishna ◽  
Krishna Kumar Dwivedi ◽  
Manoj Kumar Srivastava ◽  
A.K. Roy ◽  
D.R. Malaviya ◽  
...  
Keyword(s):  
Panicum Maximum ◽  
Meiotic Stage ◽  
Transcriptomic Data ◽  
Guinea Grass

scholarly journals Two-stage alkaline and acid pretreatment applied to sugarcane bagasse to enrich the cellulosic fraction and improve enzymatic digestibility

Detritus ◽  
2020 ◽  
pp. 106-113
Author(s):  
Longinus Ifeanyi Igbojionu ◽  
Cecilia Laluce ◽  
Edison Pecoraro
Keyword(s):  
Sugarcane Bagasse ◽  
Maleic Acid ◽  
Ethanol Yield ◽  
High Yield ◽  
Enzymatic Digestibility ◽  
Acid Pretreatment ◽  
Two Stage ◽  
Second Stage ◽  
One Stage ◽  
Naoh Pretreatment

Sugarcane bagasse (SB) is made up of cellulose (32-43%), hemicellulose (19-34%) and lignin (14-30%). Due to high recalcitrant nature of SB, pretreatment is required to deconstruct its structure and enrich the cellulosic fraction. A two-stage NaOH and maleic acid pretreatment was applied to SB to enrich its cellulosic fraction. SB used in the present study is composed of cellulose (40.4 wt%), hemicellulose (20.9 wt%), lignin (22.5 wt%) and ash (4.0 wt%). After one-stage NaOH pretreatment, its cellulosic fraction increased to 61.8 wt% and later increased to 80.1 wt% after the second-stage acid pretreatment. Lignin fraction decreased to 3.0 wt% after one-stage NaOH pretreatment and remained unaffected after the acid pretreatment step. Hemicellulose fraction decreased substantially after the second-stage pretreatment with maleic acid. Pretreated SB displayed high crystallinity index and improved enzymatic digestibility. Hydrolysates of pretreated SB contained very low amount of xylose and subsequent fermentation by Saccharomyces cerevisiae -IQAr/45-1 resulted to ethanol level of 8.94 g/L. Maximal ethanol yield of 0.49 g/g (95.8% of theoretical yield) and productivity of 0.28 g/L/h was attained. At the same time, biomass yield and productivity of 0.47 g/g and 0.27 g/L/h respectively were obtained. Two-stage NaOH and maleic acid pretreatment led to ~ two-fold increase in cellulosic fraction and enhanced the enzymatic digestibility of SB up to 70.4%. The resulted enzymatic hydrolysate was efficiently utilized by S. cerevisiae -IQAr/45-1 to produce high yield of ethanol. Thus, optimization of enzymatic hydrolysis at low enzyme loading is expected to further improve the process and reduce cost.

scholarly journals Metabolite and transcript profiling of Guinea grass (Panicum maximum Jacq) response to elevated [CO2] and temperature

Metabolomics ◽  
2019 ◽  
Vol 15 (4) ◽  
Author(s):  
Jessica M. Wedow ◽  
Craig R. Yendrek ◽  
Tathyana R. Mello ◽  
Silvana Creste ◽  
Carlos A. Martinez ◽  
...  
Keyword(s):  
Elevated Co2 ◽  
Transcript Profiling ◽  
Panicum Maximum ◽  
Guinea Grass ◽  
Elevated Co2 And Temperature

Fuel Ethanol Production from Wet Oxidised Corn Stover by S. Cerevisiae

2011 ◽  
Vol 343-344 ◽  
pp. 963-967 ◽  
Author(s):  
Zhang Qiang ◽  
Anne Belinda Thomsen
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Liquid Fraction ◽  
Raw Material ◽  
Wet Oxidation ◽  
Inhibition Effect ◽  
Simultaneous Saccharification

In order to find out appropriate process for ethanol production from corn stover, wet oxidation（195°C，15 minutes）and simultaneous saccharification and fermentation (SSF) was carried out to produce ethanol. The results showed that the cellulose recovery of 92.9% and the hemicellulose recovery of 74.6% were obtained after pretreatment. 86.5% of cellulose was remained in the solid cake . After 24h hydrolysis at 50°C using cellulase（Cellubrix L）,the achieved conversion of cellulose to glucose was 64.8%. Ethanol production was evaluated from dried solid cake and the hydrolysate was employed as liquid fraction . After 142 h of SSF with substrate concentration of 8% (W/V), ethanol yield of 73.1 % of the theoretical based on glucose in the raw material was obtained by S. cerevisiae(ordinary baker’ yeast) . The corresponding ethanol concentration and volumetric productivity were 17.2g/L and 0.121g/L.h respectively. The estimated total ethanol production was 257.7 kg/ton raw material by assuming consumption of both C-6 and C-5. No obvious inhibition effect occurred during SSF. These instructions give you the basic guidelines for preparing papers for WCICA/IEEE conference proceedings.

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