scholarly journals Treatment of osteomyelitis defects by a vancomycin-loaded gelatin/β-tricalcium phosphate composite scaffold

2018 ◽  
Vol 7 (1) ◽  
pp. 46-57 ◽  
Author(s):  
J. Zhou ◽  
X. G. Zhou ◽  
J. W. Wang ◽  
H. Zhou ◽  
J. Dong
Keyword(s):  
Controlled Release ◽  
Tricalcium Phosphate ◽  
Chronic Osteomyelitis ◽  
Composite Scaffold ◽  
Bone Defects ◽  
Therapeutic Drug ◽  
Porous Scaffolds ◽  
Composite Scaffolds ◽  
Extended Duration ◽  
Thorough Debridement

Objective In the present study, we aimed to assess whether gelatin/β-tricalcium phosphate (β-TCP) composite porous scaffolds could be used as a local controlled release system for vancomycin. We also investigated the efficiency of the scaffolds in eliminating infections and repairing osteomyelitis defects in rabbits. Methods The gelatin scaffolds containing differing amounts of of β-TCP (0%, 10%, 30% and 50%) were prepared for controlled release of vancomycin and were labelled G-TCP0, G-TCP1, G-TCP3 and G-TCP5, respectively. The Kirby-Bauer method was used to examine the release profile. Chronic osteomyelitis models of rabbits were established. After thorough debridement, the osteomyelitis defects were implanted with the scaffolds. Radiographs and histological examinations were carried out to investigate the efficiency of eliminating infections and repairing bone defects. Results The prepared gelatin/β-TCP scaffolds exhibited a homogeneously interconnected 3D porous structure. The G-TCP0 scaffold exhibited the longest duration of vancomycin release with a release duration of eight weeks. With the increase of β-TCP contents, the release duration of the β-TCP-containing composite scaffolds was decreased. The complete release of vancomycin from the G-TCP5 scaffold was achieved within three weeks. In the treatment of osteomyelitis defects in rabbits, the G-TCP3 scaffold showed the most efficacious performance in eliminating infections and repairing bone defects. Conclusions The composite scaffolds could achieve local therapeutic drug levels over an extended duration. The G-TCP3 scaffold possessed the optimal porosity, interconnection and controlled release performance. Therefore, this scaffold could potentially be used in the treatment of chronic osteomyelitis defects. Cite this article: J. Zhou, X. G. Zhou, J. W. Wang, H. Zhou, J. Dong. Treatment of osteomyelitis defects by a vancomycin-loaded gelatin/β-tricalcium phosphate composite scaffold. Bone Joint Res 2018;7:46–57. DOI: 10.1302/2046-3758.71.BJR-2017-0129.R2.

Functionalized tricalcium phosphate and poly(3-hydroxyoctanoate) derived composite scaffolds as platforms for the controlled release of diclofenac

2021 ◽  
Vol 47 (3) ◽  
pp. 3876-3883 ◽  
Author(s):  
Szymon Skibiński ◽  
Ewelina Cichoń ◽  
Katarzyna Haraźna ◽  
Elena Marcello ◽  
Ipsita Roy ◽  
...  
Keyword(s):  
Controlled Release ◽  
Tricalcium Phosphate ◽  
Composite Scaffolds

scholarly journals Gentamicin-loaded silk/nanosilver composite scaffolds for MRSA-induced chronic osteomyelitis

2019 ◽  
Vol 6 (5) ◽  
pp. 182102 ◽  
Author(s):  
Peng Zhang ◽  
Jianzhong Qin ◽  
Bo Zhang ◽  
Yi Zheng ◽  
Lingyan Yang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Formic Acid ◽  
Chronic Osteomyelitis ◽  
Antibacterial Properties ◽  
Bone Defects ◽  
Composite Scaffolds ◽  
Effective Inhibition

Methicillin-resistant Staphylococcus aureus (MRSA) often induces chronic osteomyelitis and then bone defects. Here, gentamicin-loaded silk/nanosilver composite scaffolds were developed to treat MRSA-induced chronic osteomyelitis. AgNO 3 was reduced with silk as a reducing agent in formic acid, forming silver nanoparticles in situ that were distributed uniformly in the composite scaffolds. Superior antibacterial properties against MRSA were achieved for the composite scaffolds, without the compromise of osteogenesis capacity. Then gentamicin was loaded on the scaffolds for better treatment of osteomyelitis. In vivo results showed effective inhibition of the growth of MRSA bacteria, confirming the promising future in the treatment of chronic osteomyelitis.

Improved Osteogenesis by Noggin siRNA Collagen-Bioactive Glass Composites

2021 ◽  
Vol 11 (8) ◽  
pp. 1600-1605
Author(s):  
Yanling Chen ◽  
Fan Xue ◽  
Liangjiao Chen ◽  
Zhengmao Li ◽  
Zedong Lan
Keyword(s):  
Bioactive Glass ◽  
Composite Scaffold ◽  
Bone Defects ◽  
Biological Properties ◽  
Negative Control ◽  
Regeneration Ability ◽  
Quantitative Real Time Pcr ◽  
Composite Scaffolds ◽  
Alizarin Red ◽  
Glass Composites

Collagen-bioactive glass (COL–BG) composites have significant bone regeneration ability due to the considerable osteogenic performance and desirable biological properties of collagen. In the current research, the influence of COL–BG composite scaffolds loaded with noggin siRNA on osteogenesis in MC3T3 cells have been comprehensively studied. We prepared three types of COL–BG composites scaffolds, namely, unloaded, loaded with noggin siRNA, and loaded with negative control siRNA. The effect of the aqueous extracts achieved from these scaffolds on MC3T3 cell proliferation was monitored using a CCK8 test, whilst the effect on osteogenesis was assessed with ALP (alkaline phosphatase) activity assays, quantitative real-time PCR, and Alizarin Red staining (ARS). After cells were cultured for three and five days, proliferation in the presence of the extracts of composite scaffolds was remarkably greater than in the no-scaffold blank (P < 0.05). After 14 days of cultivation, MC3T3 cells exposed to COL–BG composites scaffold loaded with noggin siRNA exhibited greater ALP performance (P < 0.05) as well as higher mRNA expression (P < 0.01) of Runx2, BSP, and ALP genes in comparison with two other composite scaffolds, whereas ARS exhibited a larger number of mineralized nodules. Overall, these outcomes illustrate that COL–BG composite scaffold loaded with noggin siRNA is effective in enhancing osteogenesis. Thus, we suggest that this material has a promising potential for bone tissue engineering and is able to locally improve bone formation in bone defects.

scholarly journals A Comparative Evaluation of the Mechanical Properties of Two Calcium Phosphate/Collagen Composite Materials and Their Osteogenic Effects on Adipose-Derived Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Qing Li ◽  
Tong Wang ◽  
Gui-feng Zhang ◽  
Xin Yu ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Composite Materials ◽  
Calcium Phosphate ◽  
Tricalcium Phosphate ◽  
Comparative Evaluation ◽  
Bone Defects ◽  
Adipose Derived Stem Cells ◽  
Composite Scaffolds

Adipose-derived stem cells (ADSCs) are ideal seed cells for use in bone tissue engineering and they have many advantages over other stem cells. In this study, two kinds of calcium phosphate/collagen composite scaffolds were prepared and their effects on the proliferation and osteogenic differentiation of ADSCs were investigated. The hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) composite scaffolds (HTPSs), which have an additionalβ-tricalcium phosphate, resulted in better proliferation of ADSCs and showed osteogenesis-promoting effects. Therefore, such composite scaffolds, in combination with ADSCs or on their own, would be promising for use in bone regeneration and potential clinical therapy for bone defects.

scholarly journals Development of Composite Scaffolds for Load-Bearing Segmental Bone Defects

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
Marcello Pilia ◽  
Teja Guda ◽  
Mark Appleford
Keyword(s):  
Composite Scaffold ◽  
Polymeric Materials ◽  
Bone Defects ◽  
Anatomical Location ◽  
Composite Scaffolds ◽  
Load Bearing ◽  
Composite Grafts ◽  
Segmental Bone ◽  
Segmental Bone Defects

The need for a suitable tissue-engineered scaffold that can be used to heal load-bearing segmental bone defects (SBDs) is both immediate and increasing. During the past 30 years, various ceramic and polymer scaffolds have been investigated for this application. More recently, while composite scaffolds built using a combination of ceramics and polymeric materials are being investigated in a greater number, very few products have progressed from laboratory benchtop studies to preclinical testing in animals. This review is based on an exhaustive literature search of various composite scaffolds designed to serve as bone regenerative therapies. We analyzed the benefits and drawbacks of different composite scaffold manufacturing techniques, the properties of commonly used ceramics and polymers, and the properties of currently investigated synthetic composite grafts. To follow, a comprehensive review ofin vivomodels used to test composite scaffolds in SBDs is detailed to serve as a guide to design appropriate translational studies and to identify the challenges that need to be overcome in scaffold design for successful translation. This includes selecting the animal type, determining the anatomical location within the animals, choosing the correct study duration, and finally, an overview of scaffold performance assessment.

Mesoporous silica nanoparticles/gelatin porous composite scaffolds with localized and sustained release of vancomycin for treatment of infected bone defects

2018 ◽  
Vol 6 (5) ◽  
pp. 740-752 ◽  
Author(s):  
Xiaojun Zhou ◽  
Weizong Weng ◽  
Bo Chen ◽  
Wei Feng ◽  
Weizhong Wang ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Sustained Release ◽  
Composite Scaffold ◽  
Mesoporous Silica Nanoparticles ◽  
Bone Defects ◽  
Porous Composite ◽  
Composite Scaffolds ◽  
Release Property ◽  
Antibiotic Release ◽  
Highly Porous

A highly porous composite scaffold with localized and sustained antibiotic release property for treatment of infected bone defects.

scholarly journals Comparison of Osteogenesis between Adipose-Derived Mesenchymal Stem Cells and Their Sheets on Poly-ε-Caprolactone/β-Tricalcium Phosphate Composite Scaffolds in Canine Bone Defects

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Yongsun Kim ◽  
Seung Hoon Lee ◽  
Byung-jae Kang ◽  
Wan Hee Kim ◽  
Hui-suk Yun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Tricalcium Phosphate ◽  
Composite Scaffold ◽  
Bone Defects ◽  
Osteogenic Potential ◽  
Mrna Levels

Multipotent mesenchymal stem cells (MSCs) and MSC sheets have effective potentials of bone regeneration. Composite polymer/ceramic scaffolds such as poly-ε-caprolactone (PCL)/β-tricalcium phosphate (β-TCP) are widely used to repair large bone defects. The present study investigated thein vitroosteogenic potential of canine adipose-derived MSCs (Ad-MSCs) and Ad-MSC sheets. Composite PCL/β-TCP scaffolds seeded with Ad-MSCs or wrapped with osteogenic Ad-MSC sheets (OCS) were also fabricated and their osteogenic potential was assessed following transplantation into critical-sized bone defects in dogs. The alkaline phosphatase (ALP) activity of osteogenic Ad-MSCs (O-MSCs) and OCS was significantly higher than that of undifferentiated Ad-MSCs (U-MSCs). TheALP, runt-related transcription factor 2, osteopontin,andbone morphogenetic protein 7 mRNA levels were upregulated in O-MSCs and OCS as compared to U-MSCs. In a segmental bone defect, the amount of newly formed bone was greater in PCL/β-TCP/OCS and PCL/β-TCP/O-MSCs/OCS than in the other groups. The OCS exhibit strong osteogenic capacity, and OCS combined with a PCL/β-TCP composite scaffold stimulated new bone formation in a critical-sized bone defect. These results suggest that the PCL/β-TCP/OCS composite has potential clinical applications in bone regeneration and can be used as an alternative treatment modality in bone tissue engineering.

scholarly journals The preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lijia Cheng ◽  
Tianchang Lin ◽  
Ahmad Taha Khalaf ◽  
Yamei Zhang ◽  
Hongyan He ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Calcium Phosphate ◽  
Tricalcium Phosphate ◽  
Weight Bearing ◽  
Bone Defects ◽  
Type I ◽  
Artificial Bone ◽  
Thermosensitive Hydrogel ◽  
Bone Repairing ◽  
Histological Staining

AbstractNowadays, artificial bone materials have been widely applied in the filling of non-weight bearing bone defects, but scarcely ever in weight-bearing bone defects. This study aims to develop an artificial bone with excellent mechanical properties and good osteogenic capability. Firstly, the collagen-thermosensitive hydrogel-calcium phosphate (CTC) composites were prepared as follows: dissolving thermosensitive hydrogel at 4 °C, then mixing with type I collagen as well as tricalcium phosphate (CaP) powder, and moulding the composites at 37 °C. Next, the CTC composites were subjected to evaluate for their chemical composition, micro morphology, pore size, Shore durometer, porosity and water absorption ability. Following this, the CTC composites were implanted into the muscle of mice while the 70% hydroxyapatite/30% β-tricalcium phosphate (HA/TCP) biomaterials were set as the control group; 8 weeks later, the osteoinductive abilities of biomaterials were detected by histological staining. Finally, the CTC and HA/TCP biomaterials were used to fill the large segments of tibia defects in mice. The bone repairing and load-bearing abilities of materials were evaluated by histological staining, X-ray and micro-CT at week 8. Both the CTC and HA/TCP biomaterials could induce ectopic bone formation in mice; however, the CTC composites tended to produce larger areas of bone and bone marrow tissues than HA/TCP. Simultaneously, bone-repairing experiments showed that HA/TCP biomaterials were easily crushed or pushed out by new bone growth as the material has a poor hardness. In comparison, the CTC composites could be replaced gradually by newly formed bone and repair larger segments of bone defects. The CTC composites trialled in this study have better mechanical properties, osteoinductivity and weight-bearing capacity than HA/TCP. The CTC composites provide an experimental foundation for the synthesis of artificial bone and a new option for orthopedic patients.

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