scholarly journals Morphological and phytochemical characterization of susceptible and resistant sengon (Falcataria moluccana) tree to gall rust disease

2019 ◽  
Vol 20 (3) ◽  
pp. 907-913
Author(s):  
DEWI RAHMAWATI ◽  
NURUL KHUMAIDA ◽  
ULFAH J SIREGAR

Abstract. Rahmawati D, Khumaida N, Siregar UJ. 2019. Morphological and phytochemical characterization of susceptible and resistant sengon (Falcataria moluccana) tree to gall rust disease. Biodiversitas 20: 907-913. Gall rust disease has severely attacked sengon tree (Falcataria moluccana Miq. Barneby & J.W. Grimes)) and it rapidly spread in tree plantations causing the decrease of wood productivity. Although sengon is widely cultivated in several areas in Indonesia, there are limited studies on secondary metabolite composition of its wood. This research was aimed to study differences on morphology, wood anatomy and secondary metabolites content of resistant and susceptible sengon tree trunk to gall rust disease. Both resistant and susceptible tree samples to gall rust were selected from the same plantation plots to minimize environmental effects. Structure of wood samples was observed using Scanning Electron Microscope (SEM) JSM-5310LV. Seven types of secondary metabolites, i.e., alkaloids, flavonoids, saponins, phenolic hydroquinones, tannins, triterpenoids, and steroids were qualitatively analyzed from powdered wood samples. Based on field observations, the gall rust fungi changed the color, texture, pores of the tree surface and decreased wood density. In microscopic observations, sengon trunk, which is resistant to gall rust disease, showed organized and regularly structured wood anatomy and did not show hyphae of the infecting fungi inside the wood. The fungi damaged susceptible sengon trunk as indicated by the dispersed and irregular structure of wood tissue, while the presence of fungal hyphae among wood cells was detected. Phytochemical analyses indicated that trunk of sengon containing flavonoids, saponins, phenolic hydroquinones, tannins, triterpenoids, and steroids, except alkaloids. Sengon trunk, which is resistant to gall rust disease, had stronger reactions of flavonoids, saponins, triterpenoids, and steroids compared to that susceptible one.

Metabolites ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 321
Author(s):  
Hazem S. Elshafie ◽  
Ippolito Camele

Burkholderia is an important bacterial species which has different beneficial effects, such as promoting the plant growth, including rhizosphere competence for the secretion of allelochemicals, production of antibiotics, and siderophores. In addition, most of Burkholderia species have demonstrated promising biocontrol action against different phytopathogens for diverse crops. In particular, Burkholderia demonstrates significant biotechnological potential as a source of novel antibiotics and bioactive secondary metabolites. The current review is concerned with Burkholderia spp. covering the following aspects: discovering, classification, distribution, plant growth promoting effect, and antimicrobial activity of different species of Burkholderia, shedding light on the most important secondary metabolites, their pathogenic effects, and biochemical characterization of some important species of Burkholderia, such as B. cepacia, B. andropogonis, B. plantarii, B. rhizoxinica, B. glumae, B. caryophylli and B. gladioli.


2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Beata Zielińska ◽  
Ewa Mijowska ◽  
Ryszard J. Kalenczuk

K-Ta mixed oxides photocatalysts have been prepared by impregnation followed by calcination. The influence of the reaction temperature (450°C–900°C) on the phase formation, crystal morphology, and photocatalytic activity in hydrogen generation of the produced materials was investigated. The detailed analysis has revealed that all products exhibit high crystallinity and irregular structure. Moreover, two different crystal structures of potassium tantalates such as KTaO3and K2Ta4O11were obtained. It was also found that the sample composed of KTaO3and traces of unreacted Ta2O5(annealed at 600°C) exhibits the highest activity in the reaction of photocatalytic hydrogen generation. The crystallographic phases, optical and vibronic properties were examined by X-ray diffraction (XRD) and diffuse reflectance (DR) UV-vis and resonance Raman spectroscopic methods, respectively. Morphology and chemical composition of the produced samples were studied using a high-resolution transmission electron microscope (HR-TEM) and an energy dispersive X-ray spectrometer (EDX) as its mode.


Sensors ◽  
2010 ◽  
Vol 10 (12) ◽  
pp. 11512-11529 ◽  
Author(s):  
Flavia Tauro ◽  
Matteo Aureli ◽  
Maurizio Porfiri ◽  
Salvatore Grimaldi

2020 ◽  
Vol 40 (6) ◽  
Author(s):  
Luciano Nobuhiro Aoyagi ◽  
Yukie Muraki ◽  
Naoki Yamanaka

Abstract Phakopsora pachyrhizi is an obligatory biotrophic fungus that causes Asian soybean rust (ASR) disease. ASR control primarily involves chemical control and the use of resistant soybean cultivars carrying an Rpp (resistance to P. pachyrhizi) gene. This study aimed to characterize the ASR resistance of three soybean Asian landraces. By screening the world core collection (WC) of soybean, which consists of 80 varieties, three landraces were identified in Southeast Asia as resistant to ASR. Genetic mapping using the F2 population derived from a cross with an ASR-susceptible variety, BRS 184, indicated that KS 1034 (WC2) has ASR resistance conferred by a single dominant resistance gene, mapped on chromosome 18, in the same region where Rpp1 was mapped previously. The BRS 184 × WC61 (COL/THAI/1986/THAI-80) F2 population, on the other hand, showed an ASR resistance locus mapped by quantitative trait locus analysis on chromosome 6, in the region where the resistance conferred by PI 416764 Rpp3 resides, with a logarithm of the odds score peak at the same position as the marker, Satt079, while the BRS 184 × WC51 (HM 39) population showed the resistance to ASR allocated between Satt079 and Sat_263 markers, also in the region where Rpp3 was mapped previously. Both WC51 and WC61 have the same infection profile as FT-2 and PI 462312 when tested against the same ASR isolate panel. These three WCs can be used in MAS programs for introgression of Rpp1 and Rpp3 and the development of ASR-resistant cultivars in the breeding program.


2019 ◽  
Vol 4 (5) ◽  
pp. 215-225
Author(s):  
Arsyik Ibrahim ◽  
M. Arifuddin ◽  
Wisnu Cahyo P ◽  
Wahyu Widayat ◽  
Mahfuzun Bone

Has been done Isolation, Characterization and Secondary Metabolite Endophytic Fungal Isolate from Peronema canescens Jack Leave and Coptosapelta tomentosa Valeton K. Heyne Root. The aim of this research is to know the number of fungal isolates, chromatogram profile and secondary metabolite group of endophytic fungal isolates from P. canencens leaves and C. tomentosa root. Characterization of endophytic fungal isolates was done macroscopically and microscopically. Identification of secondary metabolites endophytic fungal isolates were performed by chemical reaction test and TLC (Thin Layer Chromatography) method with specific spray reagents. The data of this study were obtained based on the number of endophytic fungal that can be isolated, observing macroscopic and microscopic morphological profiles, chromatogram profile and secondary metabolites of each endophytic fungal isolated. The results showed that endophytic fungal that can be isolated from P. canencens leaves four isolates, and two isolates from C. tomentosa root. Morphological profile macroscopic endophytic fungal of the six isolates showed a greenish-colored colony, white gray, clear black. Microscopic profile of each fungal isolate having spores, sprangiosphora, sporangium, conidia, hyphae and stolon. The identified secondary metabolites are: alkaloids, terpenoids, and flavonoids, and polyphenols.


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