scholarly journals Variation in morphological traits of a selection of Indonesian winged bean accessions (Psophocarpus tetragonolobus) and its analysis to assess genetic diversity among accessions

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Izmi Yulianah ◽  
Budi Waluyo ◽  
Sumeru Ashari ◽  
Kuswanto

Abstract. Yulianah I, Waluyo B, Ashari S, Kuswanto. 2020. Variation in morphological traits of a selection of Indonesian winged bean accessions (Psophocarpus tetragonolobus) and its analysis to assess genetic diversity among accessions. Biodiversitas 21: 2991-3000. In Indonesia, winged bean (Psophocarpus tetragonolobus (L.) DC.) is a traditional vegetable crop grown mainly for its edible green pods. Plant breeding programs aim to produce cultivars with high production and good nutritional qualities. The objective of this present study was to assess genetic diversity among 21 selected Indonesian winged bean lines based on observation of morphological characters. This was the first step in determining an appropriate breeding program for the development of improved vegetable cultivars. Twelve qualitative characters and eight quantitative variables were assessed for each of the 21 lines. Categorical differences among lines were observed in characters such as leaflet, pod and seed shape, pod surface texture, anthocyanin pigmentation of stem, flowers and pods. Several of these characters are useful as genetic markers, and cluster analysis of the 21 lines on the basis of qualitative characters enabled two distinct groupings to be identified. Quantitative variation across line means was also high for several of the quantitative variables (a coefficient of variation > 25% for pod length, number of pods per plant, and total pod weight per plant). Principal component analysis applied to the eight variables accounted for 86% of the total variation in just three components with eigenvalues > 1. On Component 1, the characters number of days to first open flower, pod length and pod weight were closely aligned with total weight of pods per plant. Number of pods per plant was not closely aligned with weight of pods per plant. This study has enabled broad differences between groups of lines to be categorized and has identified particular lines with characteristics that recommend them for inclusion as parents in inheritance studies designed to elucidate the contribution that individual characters make to overall productivity, attractiveness, and nutrition of this useful, high protein, vegetable species.

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Sayyidah Afridatul ◽  
MUHAMAD SYUKUR ◽  
TRIKOESOEMANINGTYAS TRIKOESOEMANINGTYAS ◽  
AWANG MAHARIJAYA

Abstract. Ishthifaiyyah SA, Syukur M, Trikoesoemaningtyas, Maharijaya A. 2021. Agro-morphological traits and harvest period assessment of winged bean (Psophocarpus tetragonolobus) genotypes for pods production. Biodiversitas 22: 1069-1075. Winged bean (Psophocarpus tetragonolobus) is an underutilized crop in Indonesia. Winged bean varieties for pods productions not only must have high productivity and nutrient contents, but also have a long harvest period. This research aimed to evaluate agro-morphological traits and harvest period of eighth-generation winged bean genotypes. Twenty genotypes of winged beans were evaluated using randomized complete block design (RCB) with three replications in Bogor, Indonesia. Several qualitative and quantitative traits were evaluated from each genotype. The results showed that there were variations among winged bean genotypes which were based on qualitative traits as well as the color of stem, calyx, corolla, pod, and pod wings. Purple coloration in the calyx of P1 and H3U genotypes was known to correspond with the purple color in stem, corolla, pod, and pod wings. The highest yield among F8 genotypes was found in H2 (6.69 to ha-1), similar to P2. However, the longest harvest period among F8 genotypes was found in H1U-2 (78 days). This study revealed that harvest period had positive correlation with the leaflet size. Based on nutrient content, H3U and L2 were considered as the genotypes with the highest protein and fiber content respectively among F8 genotypes.


2014 ◽  
Vol 1 (1) ◽  
pp. 2 ◽  
Author(s):  
Arini Maesaroh ◽  
Adi Amurwanto ◽  
Alice Yuniaty

Winged bean [Psophocarpus tetragonolobus (L.) DC] is a tropical plant that has some benefits and is very suitable to be cultivated in Indonesia. Study about diversity of winged bean is very important to support the future development of winged bean. Increased genetic diversity can be done through mutation. Gamma ray is often used for inducting mutations. One of way to observe genetic diversity resulted by molecular mutation is using RAPD method. The purpose of this study was to identify the genetic diversity of winged bean resulted by gamma ray irradiation. The method used in this study was an experiment by using RAPD technique. Samples used were plant leaves that had been induced by gamma ray with a wavelength of 20 Gy, 25 Gy, and control plants that were not induced by gamma ray. RAPD were done by ten primers that were OPA 9, OPA 10, OPA 13, OPA 18, OPB 2, 3 OPB, OPB 6, 7 OPB, OPB 10, and OPB 11. Data were analyzed using GenAlex 6.1 Program. The percentage of polymorphic loci of winged bean population control was 47,54%, while in the 20 and 25 Gy treatment were 62,30% and 54,10%, respectively. The values of genetic variation based on the calculation of allele frequencies were 0,236; 0,202 and 0,194 for treatment of 20 Gy, 25 Gy and for control plants, respectively. Meanwhile, the value of genetic distance ranged from 0.08 to 0.32.


Author(s):  
Reecha. T. Das ◽  
Purna K. Barua

Twenty three advanced breeding lines of green gram, developed at different centres under the All-India Coordinated Research Project on MULLARP, were evaluated by growing them during summer to characterize the genotypes and to assess the magnitude of genetic diversity in the genotypes. Genetic dissimilarities as revealed by Euclidean distances ranged from 2.891 to 10.494. Maximum distance was observed between ML 1354 and MH 721. Based on Euclidean distances four clusters were formed. Cluster II was the largest, and was further divided into two sub-clusters, IIA and IIB, comprising of four and nine genotypes, respectively. Genotypes MH 709 and KM 2272 belonging to cluster I were closely placed as they shared most of the characters. But they could be distinguished with the help of characters like days to 50% flowering, position of pods in the plant and growth habit. Similarly, GM 04-02 and NM 1 in sub-cluster IIA were closely grouped, and they could be distinguished based on the characters leaf size and pod length. Similarly other closely grouped genotypes were also distinguishable on the basis of some characters. It was observed from the average Euclidean distances that ML 1354, SG 1, MH 721, SG 63-14 and MH 709 were divergent genotypes. These genotypes were potential parents in green gram breeding programme.


2009 ◽  
Vol 7 (03) ◽  
pp. 230-236 ◽  
Author(s):  
G. Igrejas ◽  
V. Carnide ◽  
P. Pereira ◽  
F. Mesquita ◽  
H. Guedes-Pinto

A collection of 18 landraces of common bean (Phaseolus vulgarisL.) from northern Portugal was evaluated for both phaseolin pattern and a set of qualitative and quantitative traits. The phaseolin Contender and Tendergreen types were the most commonly occurring, with Boyaca and Huevo de Huanchaco present, but rare. The colour of the flower standard and wing, and seed shape, colour and size were all highly variable. On the basis of morphological traits, the landraces and testers clustered into two main groups. No relationship was found between phaseolin profile and any of the quantitative traits measured. Based on phaseolin patterns, the origin of Portuguese common bean populations was probably the Andean region of South America.


2019 ◽  
Vol 20 (11) ◽  
Author(s):  
Graham Eagleton

Abstract. Eagleton GE. 2019. Prospects for developing an early maturing variety of Winged Bean (Psophocarpus tetragonolobus) in Bogor, Indonesia. Biodiversitas 20: 3142-3152. To investigate the possibility of producing a more convenient, early-maturing cultivar of winged bean for vegetable growers in West Java, an early flowering accession introduced from Singapore Botanic Gardens (‘Singha’, the female parent) was crossed with a vegetatively vigorous, late-maturing, local winged bean accession (‘Bogor’, the male parent). The phenology and general performance of plants of the parental accessions and of their F1 and F2 hybrid generations were compared in the plant nursery of the Bogor Botanic Gardens (6036’ S, 106048’ E) in three successive experiments over ten months. In each of the three experimental plantings, the mean number of days to first open flower for the Bogor variety (B) was more than 40 days later than for the Singha variety (S). There was no significant difference in the average number of days to first open flower between F1 plants (S x B) and Singha plants in the first two experiments, but in the third experiment the F1 plants flowered 23 days later than the Singha parent. The phenological development of all genotypes, but especially the Bogor variety, appeared to be retarded by long daylengths. Out of nineteen F2 plants included in the third experiment, six flowered at least as early as the average of the early flowering Singha parent, nine overlapped the distribution for the F1 plants, while only two were as late as the Bogor parent. It was inferred that flowering time in this particular cross was influenced as much by dominance effects as by additive genetic effects, with early flowering dominant to late flowering. Plants in the first two experiments were severely affected by collar-rot disease with no difference between genotypes. In the third experiment, planted in a potting-mix in poly-bags that avoided the collar-rot, all plants of the Singha and most plants of the F1 and F2 generations became infected by false-rust disease Synchytrium psophocarpi (Rac.) Baumann, whereas none of the Bogor plants exhibited symptoms of false-rust. The results support the finding in a previous study by Aminah-Lubis and Sastrapradja (1981) that the resistance to false rust disease found in Bogor winged bean varieties is conferred by genes that are recessive to genes conferring susceptibility to the disease. This investigation has implications for breeding improved varieties of vegetable winged bean and for understanding the evolution of winged bean as a crop.


2017 ◽  
Vol 23 (1) ◽  
Author(s):  
R.A. PATIL ◽  
S.G. BHARAD ◽  
S.N. SAWANT

Assessment of genetic diversity in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In the view of this Forty-eight genotypes of seedling origin guava along with 1 check (L-49/Sardar) collected and conserved at germplasm block, Main Garden, Department of Horticulture, Dr. P. D. A. University, Akola were evaluated for genetic variability and diversity based on the qualitative characteristics. The genotypes were evaluated for sixteen morphological traitsviz. tree, leaf, floral and fruit traits. Results Show considerable extent of variability amongst the 49 genotypes in each traits. A sizeable amount of intrapopulation diversity recorded can be used to identify diverse parents which can be utilized in hybridization programmes.


Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1510
Author(s):  
Samuel Henrique Kamphorst ◽  
Gabriel Moreno Bernardo Gonçalves ◽  
Antônio Teixeira do Amaral Júnior ◽  
Valter Jário de Lima ◽  
Kátia Fabiane Medeiros Schmitt ◽  
...  

The identification of traits associated with drought tolerance in popcorn is a contribution to support selection of superior plants under soil water deficit. The objective of this study was to choose morphological traits and the leaf greenness index, measured on different dates, to estimate grain yield (GY) and popping expansion (PE), evaluated in a set of 20 popcorn lines with different genealogies, estimated by multiple regression models. The variables were divided into three groups: morpho-agronomic traits—100-grain weight (GW), prolificacy (PR), tassel length (TL), number of tassel branches, anthesis-silking interval, leaf angle (FA) and leaf rolling (FB); variables related to the intensity of leaf greenness during the grain-filling period, at the leaf level, measured by a portable chlorophyll meter (SPAD) and at the canopy level, calculated as the normalized difference vegetation index (NDVI). The inbred lines were cultivated under two water conditions: well-watered (WW), maintained at field capacity, and water stress (WS), for which irrigation was stopped before male flowering. The traits GY (55%) and PE (28%) were most affected by water restriction. Among the morpho-agronomic traits, GW and PR were markedly reduced (>10%). Under dry conditions, the FA in relation to the plant stalk tended to be wider, the FB curvature greater and leaf senescence accelerated (>15% at 22 days after male flowering). The use of multiple regression for the selection of predictive traits proved to be a useful tool for the identification of groups of adequate traits to efficiently predict the economically most important features of popcorn (GY and PE). The SPAD index measured 17 days after male flowering proved useful to select indirectly for GY, while, among the morphological traits, TL stood out for the same purpose. Of all traits, PR was most strongly related with PE under WS, indicating its use in breeding programs. The exploitation of these traits by indirect selection is expected to induce increments in GY and PE.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Grimar Abdiel Perez ◽  
Pumipat Tongyoo ◽  
Julapark Chunwongse ◽  
Hans de Jong ◽  
Anucha Wongpraneekul ◽  
...  

AbstractThis study explored a germplasm collection consisting of 112 Luffa acutangula (ridge gourd) accessions, mainly from Thailand. A total of 2834 SNPs were used to establish population structure and underlying genetic diversity while exploring the fruit characteristics together with genetic information which would help in the selection of parental lines for a breeding program. The study found that the average polymorphism information content value of 0.288 which indicates a moderate genetic diversity for this L. acutangula germplasm. STRUCTURE analysis (ΔK at K = 6) allowed us to group the accessions into six subpopulations that corresponded well with the unrooted phylogenetic tree and principal coordinate analyses. When plotted, the STRUCTURE bars to the area of collection, we observed an admixed genotype from surrounding accessions and a geneflow confirmed by the value of FST = 0.137. AMOVA based on STRUCTURE clustering showed a low 12.83% variation between subpopulations that correspond well with the negative inbreeding coefficient value (FIS =  − 0.092) and low total fixation index (FIT = 0.057). There were distinguishing fruit shapes and length characteristics in specific accessions for each subpopulation. The genetic diversity and different fruit shapes in the L. acutangula germplasm could benefit the ridge gourd breeding programs to meet the demands and needs of consumers, farmers, and vegetable exporters such as increasing the yield of fruit by the fruit width but not by the fruit length to solve the problem of fruit breakage during exportation.


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