scholarly journals ANALISIS RAPD KECIPIR POLONG PANJANG Psophocarpus tetragonolobus (L.) DC HASIL MUTASI IRADIASI SINAR GAMMA

2014 ◽  
Vol 1 (1) ◽  
pp. 2 ◽  
Author(s):  
Arini Maesaroh ◽  
Adi Amurwanto ◽  
Alice Yuniaty
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Population Control ◽  
Winged Bean ◽  
Plant Leaves ◽  
Psophocarpus Tetragonolobus ◽  
Tropical Plant ◽  
Gamma Ray Irradiation ◽  
And Control ◽  
Rapd Method

Winged bean [Psophocarpus tetragonolobus (L.) DC] is a tropical plant that has some benefits and is very suitable to be cultivated in Indonesia. Study about diversity of winged bean is very important to support the future development of winged bean. Increased genetic diversity can be done through mutation. Gamma ray is often used for inducting mutations. One of way to observe genetic diversity resulted by molecular mutation is using RAPD method. The purpose of this study was to identify the genetic diversity of winged bean resulted by gamma ray irradiation. The method used in this study was an experiment by using RAPD technique. Samples used were plant leaves that had been induced by gamma ray with a wavelength of 20 Gy, 25 Gy, and control plants that were not induced by gamma ray. RAPD were done by ten primers that were OPA 9, OPA 10, OPA 13, OPA 18, OPB 2, 3 OPB, OPB 6, 7 OPB, OPB 10, and OPB 11. Data were analyzed using GenAlex 6.1 Program. The percentage of polymorphic loci of winged bean population control was 47,54%, while in the 20 and 25 Gy treatment were 62,30% and 54,10%, respectively. The values of genetic variation based on the calculation of allele frequencies were 0,236; 0,202 and 0,194 for treatment of 20 Gy, 25 Gy and for control plants, respectively. Meanwhile, the value of genetic distance ranged from 0.08 to 0.32.

scholarly journals Evaluasi Keragaman Genetik Mutan Harapan Generasi MV1 Jeruk Keprok SoE (Citrus reticulata Blanco) Berdasarkan Penanda Morfologi dan ISSR

2016 ◽  
Vol 7 (2) ◽  
pp. 102
Author(s):  
Indriati Husain ◽  
Agus Purwito ◽  
Ali Husni ◽  
Kikin H. Mutaqin ◽  
Slamet Susanto
Keyword(s):  
Genetic Diversity ◽  
Gamma Irradiation ◽  
Genetic Distance ◽  
Embryogenic Callus ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Morphological Diversity ◽  
Issr Markers ◽  
Citrus Reticulata ◽  
Gamma Ray Irradiation

<p class="Abstrak"><em>ABSTRACT</em><em></em></p><p><em>Mandarin’s SoE is national <del cite="mailto:Windows%207" datetime="2016-10-31T15:54"></del>variety originated <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins>from Mount of Mutis, Sub District of SoE, of Timur Tengah Selatan (TTS) District, East Nusa Tenggara (NTT). The genetic diversity of citrus can be induced by gamma ray irradiation on embryogenic callus cells thus producing new mutants. <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del>Genetic diversity detection can be <ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>based on morphological and ISSR markers. The aim of this research was <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>to obtain information on the genetic diversity <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>on putative mutants<ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins> mandarin SoE induced by <ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>gamma ray irradiation <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del>based on morphology and markers ISSR. ISSR markers used are ISSR 1, 4, 6 and 8. Analysis of morphological diversity produced <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>a dendrogram with the level of similarity between individuals each irradiation dose 83-95% with <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>5-17% genetic distance. Dendrogram analysis based on the genetic diversity ISSR markers showed high levels of 51-100% similarity and genetic distance 0-49%. Individuals<del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del> samples obtained from<del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins> gamma irradiation, based <ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins>both morphological and ISSR markers, was different from individual's genetic make up before irradiation.</em></p><p class="Abstrak"><em>Keywords</em><em>:</em><em> cluster, </em><em>gamma ray</em><em>, genetic distance, genetic diversity</em><em>s, </em><em>similarity</em></p><p class="Abstrak"> </p><p class="Abstrak">ABSTRAK</p><p>Jeruk keprok SoE adalah jeruk varietas unggul nasional yang berasal dari Pegunungan Mutis, Kecamatan SoE, Kabupaten Timur Tengah Selatan (TTS), Provinsi Nusa Tenggara Timur (NTT). Keragaman genetik jeruk ini dapat diinduksi dengan iradiasi sinar gamma pada sel-sel kalus embriogenik untuk menghasilkan mutan yang solid. Deteksi keragaman genetik yang terbentuk dapat dilakukan secara morfologi maupun molekuler dengan marka ISSR. Tujuan Penelitian ini adalah untuk mendapatkan informasi mengenai keragaman genetik yang terjadi pada mutan harapan jeruk keprok SoE hasil iradiasi sinar gamma berdasarkan morfologi dan marka ISSR. Marka ISSR yang digunakan adalah ISSR 1, 4, 6 dan 8 pada beberapa mutan harapan jeruk keprok SoE. Analisis keragaman secara morfologi menghasilkan dendrogram dengan tingkat kemiripan antar individu masing-masing dosis iradiasi 83-95% dengan jarak genetik 5-17%. Dendrogram analisis keragaman genetik berdasar marka ISSR memperlihatkan tingkat kemiripan 51-100% dan jarak genetik 0-49%. Individu-individu sampel yang diuji hasil iradiasi gamma, baik secara morfologi dan marka ISSR, telah memiliki susunan genetik yang berbeda dari susunan genetik individu sebelum diiradiasi.</p><p>Kata kunci: grup, jarak genetik, kemiripan, keragaman, sinar gamma</p>

scholarly journals Variation in morphological traits of a selection of Indonesian winged bean accessions (Psophocarpus tetragonolobus) and its analysis to assess genetic diversity among accessions

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Izmi Yulianah ◽  
Budi Waluyo ◽  
Sumeru Ashari ◽  
Kuswanto
Keyword(s):  
Genetic Diversity ◽  
Morphological Traits ◽  
Winged Bean ◽  
Psophocarpus Tetragonolobus ◽  
Seed Shape ◽  
Open Flower ◽  
Its Analysis ◽  
Qualitative Characters ◽  
Pod Length ◽  
Selection Of

Abstract. Yulianah I, Waluyo B, Ashari S, Kuswanto. 2020. Variation in morphological traits of a selection of Indonesian winged bean accessions (Psophocarpus tetragonolobus) and its analysis to assess genetic diversity among accessions. Biodiversitas 21: 2991-3000. In Indonesia, winged bean (Psophocarpus tetragonolobus (L.) DC.) is a traditional vegetable crop grown mainly for its edible green pods. Plant breeding programs aim to produce cultivars with high production and good nutritional qualities. The objective of this present study was to assess genetic diversity among 21 selected Indonesian winged bean lines based on observation of morphological characters. This was the first step in determining an appropriate breeding program for the development of improved vegetable cultivars. Twelve qualitative characters and eight quantitative variables were assessed for each of the 21 lines. Categorical differences among lines were observed in characters such as leaflet, pod and seed shape, pod surface texture, anthocyanin pigmentation of stem, flowers and pods. Several of these characters are useful as genetic markers, and cluster analysis of the 21 lines on the basis of qualitative characters enabled two distinct groupings to be identified. Quantitative variation across line means was also high for several of the quantitative variables (a coefficient of variation > 25% for pod length, number of pods per plant, and total pod weight per plant). Principal component analysis applied to the eight variables accounted for 86% of the total variation in just three components with eigenvalues > 1. On Component 1, the characters number of days to first open flower, pod length and pod weight were closely aligned with total weight of pods per plant. Number of pods per plant was not closely aligned with weight of pods per plant. This study has enabled broad differences between groups of lines to be categorized and has identified particular lines with characteristics that recommend them for inclusion as parents in inheritance studies designed to elucidate the contribution that individual characters make to overall productivity, attractiveness, and nutrition of this useful, high protein, vegetable species.

scholarly journals EVALUATION AND SELECTION FENOTYPE OF GARUT PLANT (Maranta arundinacea L.) WITH GAMMA RAY RADIATION TREATMENTS

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Puspita Deswina ◽  
Sri Indrayani ◽  
Ambar Yuswi Perdani ◽  
Enung S Mulyaningsih
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Morphological Changes ◽  
Morphological Characteristics ◽  
Optimal Dose ◽  
Alternative Source ◽  
Randomized Design ◽  
Gamma Ray Irradiation ◽  
Potential Alternative ◽  
Radiation Treatments

ABSTRACT Garut (Maranta arundinaceae L.) is one crop a potential alternative source of carbohydrate that has enormous potential to be developed. Food made from arrowroot flour has the advantage that is easy to digest up to very good for health. These plants are generally propagated vegetative, so it has a narrow genetic diversity. To increase the genetic diversity of arrowroot plants, gamma ray irradiation was performed at the Center for Radiation and Isotopes, BATAN, at a dose of 10 to 140 Gy with intervals of 10.  Analysis of morphological changes and the selection was done by observing the characteristic changes of the plant began generating plant shoots up to a maximum plant growth or plant produces flowers. The research objective was to determine the optimal dose of irradiation in determining the LD 50 for expanding the arrowroot plant genetic diversity. The method used was completely randomized design with 15 treatments and two replications. Based on the results revealed that the radiation could be affect to the morphological characteristics of plants such as leaves, number of shoots, plant height and tubers production. Key words: gamma ray radiation, alternative food, Garut plant (Maranta arundinaceae L).

scholarly journals Pengaruh Iradiasi Sinar Gamma terhadap Pertumbuhan Stek Tunas Apikal dan Aksilar Kopi Arabika

2020 ◽  
Vol 7 (3) ◽  
pp. 137
Author(s):  
Meynarti Sari Dewi Ibrahim ◽  
Enny Randriani
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Energy Agency ◽  
Arabica Coffee ◽  
Apical Shoot ◽  
Low Genetic Diversity ◽  
Planting Material ◽  
Randomized Design ◽  
Gamma Ray Irradiation ◽  
Ld50 Value

<p><em>The Arabica coffee is predominantly self-pollinated plants thereby contributing to low genetic diversity. The effort to increase the genetic diversity of Arabica coffee through crossing strategy is time-consuming, and induce mutation is necessary to enhance the rate of genetic variation. The aims of this study were to observe the effect of gamma-ray irradiation on the growth of apical and axillary bud cuttings and to determine the value of LD<sub>50</sub> on apical cuttings and Arabica coffee axillaries. The study was conducted at the Tissue Culture Laboratory, Industrial and Beverage Crops Research Institute, from January to December in 2018.. The planting material that was irradiated was Arabica coffee plantlets resulting from somatic embryogenesis propagation. Irradiation is carried out at the National Nuclear Energy Agency. The irradiated plantlets were cut and subcultured onto MS medium without growth regulators, 30 g L<sup>-1</sup> sucrose, and 2.5 g L<sup>-1</sup> phytagel were added. The design used a completely randomized design with 10 replications. The treatments tested were the dose of gamma-ray irradiation (0, 10, 20, 30, 40, and 50 Gy). The results showed that gamma-ray irradiation had an effect on all observed parameters. The mortality percentage of apical shoot cuttings began to be found at 30 Gy, while axillary cuttings at 20 Gy increased with an increasing dose of gamma-ray irradiation. The number of shoots and leaves varied between irradiation doses on both apical and axillary cuttings. The LD50 value of apical shoot cuttings was 36.80 Gy, while axillary cuttings were 22.24 Gy</em></p>

scholarly journals Induksi Mutasi Kara Benguk (Mucuna pruriens L.) Menggunakan Iradiasi Sinar Gamma

2020 ◽  
Vol 22 (2) ◽  
pp. 105
Author(s):  
Siti Hartati Yusida Saragih ◽  
Khairul Rizal ◽  
Kamsia Dorliana Sitanggang
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mucuna Pruriens ◽  
Mutation Induction ◽  
High Genetic Diversity ◽  
Group Design ◽  
Planting Material ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves

<p>Breeding kara benguk with mutation induction techniques using gamma ray radiation is expected to produce genetic diversity. The purpose of the study is to determine the value of Lethal Dose 50 (LD50) and obtain the genetic diversity of the kara benguk plant. The research was conducted at PAIR BATAN and Agrotechnology experiment, Universitas Labuhanbatu from May-July 2020. The planting material used was kara benguk seeds. The method used was Randomized Complete Group Design (RCGD) with one factor which was dose of irradiation with six levels (0, 200, 250, 300, 350 and 400 Gy) and three replications. Each replication consisted of 20 plants, so there are a total of 360 experimental plants. The results showed that the value of LD50 kara benguk in 3 weeks after planting was 281,472 Gy. Gamma ray irradiation increases the high genetic diversity of plants and the number of leaves at a dose of 350 Gy.</p>

scholarly journals Keragaan Anggrek Persilangan ♀ Vanda Celebica X ♂ Vanda Dearei Hasil Iradiasi Sinar Gamma

2017 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Sri Hartati ◽  
Ahmad Yunus ◽  
Fajar Nugroho
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Color Change ◽  
Leaf Length ◽  
Leaf Color ◽  
Color Changes ◽  
Dose Irradiation ◽  
Randomized Design ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation

<p>Variation on orchid is an advantage that allows plant as breeding material. Orchid in plant breeding has a target to increase plant genetic diversity character who favored by the consumer. Efforts to increase genetic diversity among others by artificial mutation using gamma ray irradiation. This research aims to study the influence of gamma ray irradiation dose against the performance of morphology and growth of crossbred orchids ♀Vanda celebica x ♂Vanda dearei. Research using completely randomized design single factor that is dose gamma ray irradiation 0 Gray, 10 Gray, 20 Gray, 30 Gray, 40 Gray and 50 Gray. Data were analyzed descriptively, compared plant irradiation treatments with control plants to determine the effect of irradiation on morphology and growth plant. The results showed dose irradiation treatment increased the diversity of plant height, leaf amount, leaf length, leaf color changes, the amount and length of roots. 10 Gray dose irradiation increases as height and length plant leave. 30 Gray dose irradiation increase in the number of leaves. Leaf color change occurs at 20 irradiation treatments Gray and 50 Gray.</p>

scholarly journals Radiosensitivitas Kalus Embriogenik Kopi Robusta Bp 436 Terhadap Iradiasi Sinar Gamma

2019 ◽  
Vol 6 (1) ◽  
pp. 41
Author(s):  
Meynarti Sari Dewi Ibrahim ◽  
Enny Randriani ◽  
Laela Sari ◽  
Anne Nuraini
Keyword(s):  
Genetic Diversity ◽  
Somatic Embryos ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mutation Induction ◽  
High Genetic Diversity ◽  
Robusta Coffee ◽  
Randomized Design ◽  
Young Leaves ◽  
Gamma Ray Irradiation

<p><em>High genetic diversity is one factor that determines the success of plant breeding. Mutation induction by gamma ray irradiation is one method to improve plant genetic diversity. This study aimed to 1) obtain growth regulators composition suitable in inducing embryogenic callus, 2) determine the effect of gamma ray irradiation on the growth and development of somatic embryos, and 3) obtain lethal dose (LD) <sub>20</sub> and <sub>50</sub> values in Robusta coffee  BP 436. The study was conducted at the Tissue Culture Laboratory, Industrial and Beverage Crops Research Institute,  from May 2017 to December 2018. Explants used were young leaves of Robusta coffee BP 436. Callus induction used ½ MS media with 2,4-D (4.52 μM) and 2-iP (0.00; 4.93; 9.86; 14.79; and 19.72 μM) treatment. Mutation induction was performed using gamma radiation dosed at 0, 10, 20, 30, 40, and 50 Gy treatments. The regeneration media was ½ MS containing GA<sub>3</sub> (0 and 1 mg/l). The study used a complete randomized design with 10 replications. The results showed the best combination of plant growth regulator to induce the callus was 2.4-D 4.52 μM + 2-iP 19.72 μM. The fresh weight of cultures was inhibited above 30 Gy, whereas the number of somatic embryos decreased at doses above 10 Gy. Addition of GA<sub>3</sub> 1 mg/l in regeneration media increased the number of somatic embryos in torpedo phase, but not in gamma irradiation exposed calluses. The LD<sub>20</sub> and LD<sub>50</sub> of Robusta coffee BP 436 are 16.81 and 28.52 Gy, respectively.</em></p>

scholarly journals Morphology Performance On Six Black Rice Accessions (Oryza Sativa L.) In M1 Generation Irradiated By Gamma Rays

2021 ◽  
Vol 3 (2) ◽  
pp. 62-67
Author(s):  
Roza Yunita ◽  
Siti Nurhidayah
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Gamma Rays ◽  
Gamma Ray ◽  
Oryza Sativa L ◽  
Seedling Height ◽  
Black Rice ◽  
Germination Characteristics ◽  
Gamma Ray Irradiation ◽  
Materials Used

Increasing the genetic diversity of plants can be done through physical mutations with gamma-ray irradiation. This study aims to determine gamma-ray irradiation on the growth of six M1 generation black rice accessions. This research was conducted in May-October 2019 at the Experimental Rice Fields of the Tasikmalaya Perjuangan University, West Java. The materials used in this study were six accessions from Tasikmalaya (PH, PH2, PH3, PH5, PH7, and PH8) without 0 Gy irradiation and with 200 Gy gamma-ray irradiation of M1 generation. The results showed that gamma-ray irradiation reduced germination characteristics, seedling height, and flowering age. Some of the accessions showed different effects; there were a decrease and an increase in the characters of root length, plant height, number of productive tillers, number of unproductive tillers, and the total number of tillers.

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