scholarly journals Penapisan Cendawan Trichoderma spp. untuk Pengendalian Phytophthora infestans secara in vitro

2016 ◽  
Vol 12 (3) ◽  
pp. 96
Author(s):  
Susiana Purwantisari ◽  
Achmadi Priyatmojo ◽  
Retno Peni Sancayaningsih ◽  
Rina Sri Kasiamdari
Keyword(s):  
Growth Inhibition ◽  
Phytophthora Infestans ◽  
Pathogenic Fungus ◽  
Culture Method ◽  
Inhibition Zone ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Suppressive Soil ◽  
Blight Disease

Late blight disease on potato caused by a plant pathogenic fungus (Phytophthora infestans)  is the most important disease in Indonesia.  The use of antagonist fungi Trichoderma is an environmentally friendly technology to control the potato disease. The purpose of this study was to determine the ability of Trichoderma spp. to control P. infestans in vitro. Trichoderma spp. have been isolated from suppressive soil at central potato areas in district of Lembang, Bandung, West Java Province. To determine the inhibition ability of Trichoderma spp against P. infestans, a dual culture method was performed.  Variable observed was inhibition zone of Trichoderma spp. against P. infestans. The results showed that 9 isolates of Trichoderma were successfuly isolated from suppressive soil, i.e. T. viride (2 isolates), T. atroviride (1 isolate), T. harzianum (1 isolate) and T. aureoviride (5 isolates). All the Trichoderma isolates revealed growth inhibition abality against P. infestans and. The highest growth inhibition (68.6%) was observed by T. viride isolate ( Ti 9).

scholarly journals Mycoparasitic capabilities of diverse native strain of Trichoderma spp. against Fusarium oxysporum f. sp. lycopersici

2016 ◽  
Vol 8 (2) ◽  
pp. 769-776
Author(s):  
Lakshman Prasad ◽  
Sorabh Chaudhary ◽  
Sushma Sagar ◽  
Akash Tomar
Keyword(s):  
Growth Inhibition ◽  
Fusarium Oxysporum ◽  
Biological Control Agent ◽  
Culture Method ◽  
Colony Growth ◽  
Limiting Factor ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Native Strain

The Fusarium wilt of tomato (Lycopersicon esculentum Mill.) caused by Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder and Hansen is recognised as one of the most devastating disease and major yield limiting factor in tomato growing regions worldwide. For eco-friendly and sustainable management of the disease, 19 Trichoderma native isolates belonging to 3 species of the genus, T. harzianum, T. asperellum and T. virens were evaluated in vitro against the pathogen using dual culture method. Out of 19 isolates, 8 isolates showed mycoparasitism, 8 isolates showed antibiosis and remaining showed lysis. Microscopic observations of Fusarium oxysporum f. sp. lycopersici (FOL) growth in dual cultures revealed that growth inhibition occurred just before near to contact with the antagonist. All T. harzianum isolates tested exhibited coiling around the hyphae of FOL. Isolates of T. harzianum, showed good coiling and growth inhibition of the pathogen. The T. harzianum strains did not differ in coiling pattern and gave somewhat equal coiling performances. Strains of T. asperellum, showed coiling but the coiling pattern of all these strains was different. Only one strain of T. virens showed coiling out of 2 strains. Among them T. harzianum (SVPUTh91) showed the best performance in vitro as biological control agent against FOL followed by T. asperellum and T. virens, resulting in 83, 73 and 65% reduction in colony growth, respectively.

scholarly journals Suppression of Stem-End Rot on Avocado Fruit Using Trichoderma spp. in the Central Highlands of Kenya

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
E. K. Wanjiku ◽  
J. W. Waceke ◽  
J. N. Mbaka
Keyword(s):  
Growth Inhibition ◽  
Fungal Pathogen ◽  
Mycelial Growth ◽  
Culture Technique ◽  
Global Market ◽  
Dual Culture ◽  
Postharvest Diseases ◽  
Trichoderma Spp ◽  
Lasiodiplodia Theobromae

Demand for organic avocado fruits, together with stringent food safety standards in the global market, has made producers to use alternative, safe, and consumer-friendly strategies of controlling the postharvest fungal disease of avocado fruits. This study assessed the in vitro efficacy of Trichoderma spp. (T. atroviride, T. virens, T. asperellum, and T. harzianum) against isolated avocado stem-end rot (SER) fungal pathogens (Lasiodiplodia theobromae, Neofusicoccum parvum, Nectria pseudotrichia, and Fusarium solani) using a dual culture technique. The Trichoderma spp. were also evaluated singly on postharvest “Hass” avocado fruits. Spore suspension at 5 × 104 conidial/ml of the Trichoderma spp. was applied on the avocado fruits at three time points, twenty-four hours before the fungal pathogen (preinoculation), at the same time as the fungal pathogen (concurrent inoculation), and 24 hours after the fungal pathogen (postinoculation). In the in vitro study, T. atroviride showed the highest mycelial growth inhibition against N. parvum (48%), N. pseudotrichia (55%), and F. solani (32.95%), while T. harzianum had the highest mycelial growth inhibition against L. theobromae. Trichoderma asperellum was the least effective in inhibiting the mycelial growth of all the pathogens. Similarly, T. virens showed the highest mycelial growth inhibition against N. pseudotrichia at 45% inhibition. On postharvest “Hass” fruits, T. atroviride showed the highest efficacy against N. parvum, N. pseudotrichia, and F. solani in all the applications. Trichoderma virens and T. harzianum were most effective against all the pathogens during postinoculation, while Lasiodiplodia theobromae was best controlled by T. virens, T. harzianum, and T. asperellum during postinoculation. Both T. atroviride and T. harzianum present a potential alternative to synthetic fungicides against postharvest diseases of avocado fruits, and further tests under field conditions to be done to validate their efficacy. The possibility of using Trichoderma spp. in the management of SER on avocado fruits at a commercial level should also be explored.

scholarly journals EKSPLORASI DAN IDENTIFIKASI CENDAWAN ANTAGONIS TERHADAP JAMUR AKAR PUTIH (Rigidoporus lignosus) PADA TANAMAN PALA (Myristica fragrans Houtt)

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Nana Ariska ◽  
Lola Adres Yanti ◽  
Chairudin Chairudin
Keyword(s):  
Economic Value ◽  
Culture Method ◽  
Dilution Method ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Pests And Diseases ◽  
Myristica Fragrans ◽  
Rigidoporus Lignosus ◽  
Myristica Fragrans Houtt

Nutmeg (Myristica fragrans Houtt) is a multipurpose plantation commodity crop that almost all parts of the plant have strategic economic value to be cultivated. In Indonesia there are the largest nutmeg commodity centers, including the Maluku islands, North Sulawesi and Aceh. The biggest problem with nutmeg plants is the attack of pests and diseases. One of the most severe diseases in nutmeg is white root mushroom (Rigidoporus lignosus). This study aims to 1) determine the fungus of white root fungus antagonists (Rigodoporus lignosus) from the Aceh nutmeg rhizosphere (Myristica fragrans Houtt), 2) identify the content of antibiotic fungus JAP antagonist compounds in Aceh nutmeg plants. The study was conducted by taking soil samples from nutmeg plants at three nutmeg plantations in South Aceh. The antagonist fungus isolation activity uses a dilution method. Isolation of JAP from infected roots using direct planting method. Furthermore, it is identified macroscopically and microscopically. The antagonist test was carried out using the dual culture method with RAL. From the rhizosphere in the nutmeg area, 5 candidate antagonist isolates were obtained, having high in vitro antagonism ability to the pathogen R. lignosus. Identification results showed that isolates 1, 2 and 3 were Trichoderma spp, and isolates 4 and 5 were Gliocladium virens. Keywords: In vitro, the content of antibiotic compounds, Aceh nutmeg, Rigidoporus lignosus, antagonistic test

scholarly journals In vitro Antagonistic Mechanisms of Trichoderma spp. and Talaromyces flavus to Control Gaeumannomyces graminis var. tritici the Causal Agent of Wheat Take-all Disease

2015 ◽  
Vol 3 (8) ◽  
pp. 629
Author(s):  
Seddighe Mohammadi ◽  
Leila Ghanbari
Keyword(s):  
Pathogenic Fungus ◽  
Causal Agent ◽  
Gaeumannomyces Graminis ◽  
Dual Culture ◽  
Mycelium Growth ◽  
Trichoderma Spp ◽  
Talaromyces Flavus ◽  
Gaeumannomyces Graminis Var Tritici ◽  
Take All

Wheat take-all disease caused by Gaeumannomyces graminis var. tritici has recently been detected in different regions of Iran. With respect to biocontrol effect of Trichoderma spp. on many pathogenic fungi, seven isolates of Trichoderma and four isolates of Talaromyces were in vitro evaluated in terms of their biological control against the disease causal agent. In dual culture test the five isolates showed efficient competition for colonization against pathogenic fungus and the highest percentages of inhibition belonging to Talaromyces flavus 60 and Talaromyces flavus 136 were 59.52 and 57.61%, respectively. Microscopic investigations showed that in regions where antagonistic isolates and Gaeumannomyces graminis var. tritici coincide, hyphal contact, penetration and fragmentation of Gaeumannomyces graminis var. tritici were observed. Investigating the effect of volatile and non-volatile compounds at 10 ml concentration showed that the highest inhibition percentage on mycelium growth of the pathogen caused by T. harzianum (44.76%) and T. longibrachiatum (52.38%) respectively.

scholarly journals Morphological and Molecular Characterization of Trichoderma spp. from Rhizosphere Soil and Their Antagonistic Activity against Fusarium spp.

2021 ◽  
pp. 100-112
Author(s):  
Jaygendra Kumar ◽  
Mukesh Kumar ◽  
Akash Tomar ◽  
. Vaishali ◽  
Pushpendra Kumar ◽  
...  
Keyword(s):  
Plant Pathogens ◽  
Rhizosphere Soil ◽  
Pcr Amplification ◽  
Its Region ◽  
Culture Method ◽  
Dual Culture ◽  
Fusarium Spp ◽  
Trichoderma Spp ◽  
Trichoderma Species

Trichoderma species are well known for their biocontrol activity which colonize many soil and tuber-borne and foliage plant pathogens. In this study, 12 native isolates of Trichiderma spp were collected from various crop rhizosphere soil samples and characterized them phenotypically based on morphological and cultural features and genotypically based on sequence analysis of internal transcribed spacer (ITS) region-PCR amplification. The results obtained from phenotypic and genotypic observation revealed that isolates were belonged to five different species namely T. asperellum, T. harzianum, T. longibrachiatum, T. koningii and T. koningiopsis. All Trichoderma isolates produced ~600 bp amplicon and phylogenetic analysis revealed that all isolates were grouped with respective species. Further, the antagonistic potential of all the isolates was evaluated against Fusarium spp. following in vitro dual culture method. The results showed that isolates of T. harzianum exhibited maximum growth inhibition activity. The highest rate of inhibition was recorded with T. harzianum isolate TBT6 (87.1%) followed by TBT7 (82.2%), while the least inhibition was observed in T. longibrachiatum isolate TBT10 (59.7%) after 7 days of incubation. The antagonistic T. harzianum isolate TBT6 can be used for development of Trichoderma based bio-formulation and served as bio-control agent against Fusaium spp. under field conditions.

scholarly journals EXPLORATION AND SELECTION OF RHIZOBACTERIA THAT INHIBIT PHYTOPHTHORA CAPSICI IN VITRO

2018 ◽  
Vol 18 (1) ◽  
pp. 83
Author(s):  
Aulia Zakia ◽  
Satriyas Ilyas ◽  
Candra Budiman ◽  
Syamsuddin . ◽  
Dyah Manohara
Keyword(s):  
Phytophthora Capsici ◽  
Culture Method ◽  
Healthy Plant ◽  
Dual Culture ◽  
Phytophthora Blight ◽  
Heated Sample ◽  
Resistant Varieties ◽  
Blight Disease ◽  
Selection Of

Exploration and Selection of Rhizobacteria that Inhibit Phytophthora capsici in vitro. Phytophthora capsici, a seed borne and the soil borne fungal pathogen is the cause of phytophthora blight on chili. The disease is difficult to control because of the resistant varieties unavailability in Indonesia. The aimed was to obtain isolates of rhizobacteria which has the ability to inhibit P. capsici in vitro. Rhizobacteria exploration was conducted in the chili production center in East Java (Malang, Batu, and Kediri) and West Java (Bogor). In one location, chili plant that had symptoms of phytophthora blight disease and a healthy plant next to it were chosen as samples to isolate P. capsici and the rhizobacteria. The rhizobacteria were isolated on NA, TSA, and TSAP (TSA with heated sample). Samples of diseased plants were used in isolation of P. capsici on V8 agar. The inhibition and compatibility of the rhizobacteria to inhibit P. capsici in vitro were tested by dual culture method. In this experiment, it was obtained 252 isolates of rhizobacteria and one isolate of P. capsici. Isolates of rhizobacteria with high to medium inhibition were E1, E3C2, and F2B1 respectively. All three isolates were then combined and tested against P. capsici in vitro. The highest inhibition was indicated by four isolate and combination of isolates, which were E1 isolate (58%), the combination of E1 + E3C2 isolates (58%), E1 + F2B1 (60%) and E1 + E3C2 + F2B1 (58 %).

scholarly journals In vitro evaluation of biocontrol agents and fungicides on wood decay fungiganoderma associated with mortality of tree legumes

2017 ◽  
Vol 6 (2) ◽  
pp. 31-35 ◽  
Author(s):  
M Bhadra ◽  
A Khair ◽  
MA Hossain ◽  
MM Sikder
Keyword(s):  
Growth Inhibition ◽  
Mycelial Growth ◽  
Biocontrol Agent ◽  
Wood Decay ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Decay Fungi ◽  
Trichoderma Species ◽  
Wood Decay Fungi

An experiment was conducted to isolate a number of biocontrol agent- Trichoderma spp. from infected spawn packets of oyster mushroom at National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh. These bio-control agents were used as antagonist against four wild wood decay fungi of Ganoderma, viz., G. lucidum-1, G. lucidum-2, G. lucidum-3, G. applanatum and two cultivated G. lucidum-4, G.lucidum-6 under in vitro condition. An in vitro trial of Trichoderma spp. against Ganoderma were performed by dual culture, by treating with volatile, non-volatile and naturally untreated metabolites of bio-control agents. In dual culture, all the Trichoderma species showed 70- 100% mycelia inhibition of G. lucidum-1 and G. lucidum-2, 55.6-100% inhibition of G. lucidum-3, 20-66.7% of G. applanatum, 100% of G. lucidum-5, 75-100% of G. lucidum-6. Effects of heat killed extracts of Trichoderma spp. on growth of G. lucidum-2 (wild) and G. lucidum-6 (cultivated) were also evaluated. Fungicides Bavistin and Dithane M-45 were also used to investigate the mycelial growth inhibition of Ganoderma spp.Int. J. Agril. Res. Innov. & Tech. 6 (2): 31-35, December, 2016

scholarly journals PEMANFAATAN BIOFUNGISIDA BERBAHAN AKTIF Trichoderma spp. UNTUK PENGENDALIAN PENYAKIT BUSUK BUAH KAKAO

2016 ◽  
Vol 3 (1) ◽  
pp. 28
Author(s):  
Winda Nawfetrias ◽  
Eka Nurhangga ◽  
Sutardjo .
Keyword(s):  
Active Ingredient ◽  
Low Cost ◽  
Pathogenic Fungi ◽  
Culture Method ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Cocoa Production ◽  
Black Pod

Cocoa black pod rot is caused by pathogenic fungi, Phytophtora palmivora, which decrease the cocoa production up to 90%. The use of biological control agents, Trichoderma spp., is one of the promising P. palmivora controllers since it is low-cost, easily found and safe for the environment. The aims of this research were to understand the compatibility, antagonistic and effectiveness of biofungicide containing active ingredient of Trichoderma spp. against P. palmivora in vitro and to test the effective concentration of biofungicide containing active ingredient of T. asperellum to control P. palmivora in vitro and in vivo. T. asperellum, T. harzianum, and T. viride were grown together on PDA medium to test their compatibility. Antagonistic and effectiveness test of Trichoderma spp. against P. palmivora were tested using the in vitro dual culture method. The effectiveness of T. asperellum biofungicide was tested in vivo on cocoa pot. Compatibility test showed that all three species were compatible and the best effectiveness showed by the combination of T. asperellum and T. viride. The result also showed that T. asperellum biofungicide had an ability to inhibit P. palmivora.   Keywords: Trichoderma spp., effectivity, compatibility, antagonistic, biofungicide  ABSTRAKPenyakit busuk buah kakao disebabkan cendawan patogen Phytophtora palmivora, yang dapat menurunkan produksi kakao sampai 90%. Penggunaan agensia pengendali hayati (APH), Trichoderma spp., merupakan salah satu pengendalian P. palmivora yang menjanjikan karena murah, mudah didapat dan aman terhadap lingkungan. Penelitian ini bertujuan mengetahui kesesuaian, antagonistik, dan efektivitas biofungisida berbahan aktif Trichoderma spp. secara in vitro. Di samping itu juga bertujuan mengetahui konsentrasi efektif biofungisida berbahan aktif T. asperellum untuk mengendalikan P. palmivora secara in vitro dan in vivo. T. asperellum, T. harzianum, dan T. viride, ditumbuhkan bersama pada media PDA untuk mengetahui kesesuaian antarspesies. Antagonistik dan efektivitas Trichoderma spp. terhadap P. palmivora secara in vitro diuji menggunakan metode dual culture. Biofungisida berbahan aktif T. asperellum diuji efektivitasnya secara in vivo pada buah kakao. Hasil uji kesesuaian menunjukkan bahwa ketiga spesies yang diuji berkesesuaian dan efektifitas terbaik ditunjukkan pada kombinasi T. asperellum dan T. viride. Hasil penelitian juga menunjukkan bahwa biofungisida berbahan aktif T. asperellum dengan konsentrasi tertinggi terbukti dapat menghambat pertumbuhan P. palmivora.Kata kunci: Trichoderma spp., efektivitas, kesesuaian, antagonis, biofungisida

scholarly journals Antagonistic screening of Trichoderma spp. isolated from patchouli rhizosphere

2022 ◽  
Vol 951 (1) ◽  
pp. 012021
Author(s):  
Muzakir ◽  
Hifnalisa ◽  
J. Jauharlina ◽  
Rina Sriwati
Keyword(s):  
Plant Pathology ◽  
Culture Method ◽  
Antagonistic Activity ◽  
Potato Dextrose Agar ◽  
Dual Culture ◽  
Fusarium Spp ◽  
Trichoderma Spp ◽  
Pogostemon Cablin

Abstract The objective of this research was to determine the antagonistic activity of Trichoderma spp. isolated from patchouli rhizosphere (Pogostemon cablin Benth.). Another objective was to perform antagonistic screening of these fungi to inhibit the growth of the wilted pathogen Fusarium spp. In vitro research was conducted in the Laboratory of Plant Pathology, Universitas Syiah Kuala, from January to June 2020. The study used a completely randomised design with five treatments and three replications. The antagonistic screening was carried out by using the dual culture method of Trichoderma spp. against Fusarium spp. with the medium of Potato Dextrose Agar (PDA). The result showed that five isolates of Trichoderma have different antagonistic percentages in inhibiting the Fusarium. The highest antagonistic activity was found from isolate 2 and the lowest value was shown by isolate 3.

In-vitro and greenhouse management of banded leaf and sheath blight (BLSB) of maize, at Rampur, Chitwan, Nepal

2020 ◽  
Vol 5 (6) ◽  
Author(s):  
GC Sagar ◽  
Manandahar HK ◽  
Shrestha S ◽  
Bhusal K
Keyword(s):  
Growth Inhibition ◽  
Disease Severity ◽  
Block Design ◽  
Maximum Growth ◽  
Separate Experiment ◽  
Root Weight ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Pathology Laboratory

The research included in-vitro experiments on the effect of the fungal antagonist (Trichoderma spp.) and fungicide (carbendazim) against BLSB pathogen (Rhizoctonia solani); and greenhouse experiment for the management of BLSB using antagonists (Trichoderma and Pseudomonas) and a fungicide (carbendazim) in different combinations. The in-vitro and greenhouse experiments were done at the plant pathology laboratory and greenhouse of Agriculture and Forestry University, Rampur, Chitwan. Of the isolates of Trichoderma evaluated against the BLSB pathogen in dual culture, the Trichoderma isolated from between healthy and diseased maize plants gave the maximum growth inhibition percent of the pathogen at 24 (19.86±0.52), 48 (51.63±0.40) and 72hours (72.78±1.11). Carbendazim at 10 ppm completely inhibited the growth of both the pathogen and Trichoderma on PDA medium, while at 1 ppm, the growth inhibition of the pathogen was (54.22±0.89) percent and of Trichoderma was (50.22±3.11) percent. The management experiment in a glasshouse with seven treatments was done in a randomized complete block design with three replications. Carbendazim alone gave the highest disease control (59.73%) with the lowest disease severity (28.67±0.67) and AUDPC per day (803.33±33.33). A combination of treatments alternately with Trichoderma and carbendazim also had a significant effect on reducing disease severity. In a separate experiment, Trichoderma with and without pathogen gave significantly higher shoot length, root length, shoot weight and root weight when compared with untreated control.

Sign in / Sign up

Export Citation Format

Share Document