scholarly journals The effect of vitamin C toward the improvement of spermatogenesis and spermatozoa quality on mice (Mus musculus L.) after the addition of tobacco extract (Nicotiana tabacum L.)

2003 ◽  
Vol 1 (1) ◽  
pp. 13-19
Author(s):  
TITISARI NUGRAHENI ◽  
OKID PARAMA ASTIRIN ◽  
TETRI WIDIYANI

The aim of this research was to describe the ability of vitamin C in improving the spermatogenesis and spermatozoa quality of mice after the treatment of tobacco (Nicotiana tabacum L.) extract. The framework of the research was that it was already known that vitamin C was one of the anti-oxidant, which was able to counter free radical of the nicotine obtained in tobacco extract in improving the spermatogenesis. The research would be conducted by grouping the mice into 6 groups, and each group consists of 4 repetitions in 35 days. The first group was as control, the second group was treated with C vitamin in the dosage of 0.024 mg/g of body weight, the third group was treated with tobacco extract in the dosage of 0,121 mg/g of body weight, the fourth group was treated with tobacco extract in the dosage of 0,121 mg/g of body weight and C vitamin of 0,012 mg/g of body weight, the fifth group was treated with tobacco extract in the dosage of 0,121 mg/g of body weight and C vitamin of 0,024 mg/g of body weight, and the sixth group was treated with tobacco extract in the dosage of 0,121 mg/g of body weight and C vitamin of 0,036 mg/g of body weight. Based on the data analysis, it can be concluded that tobacco extract treatment could decrease spermatogenesis quality and spermatozoa quality of mice (Mus musculus L.). C Vitamin could improve spermatogenesis and spermatozoa quality of mice after tobacco extract treatment. The optimal dosage of C vitamin treatment to improve the spermatogenesis and the quality of spermatozoa after the addition of tobacco extract treatment was 0,024 mg/g of weight.


2013 ◽  
Vol 13 (2) ◽  
Author(s):  
D. Setiadi Dan S. Bachri

AbstrakIndonesia memiliki banyak tumbuhan berpotensi obat salah satunya bisa dijadikan sebagai obatkontrasepsi tradisonal yang biasa digunakan untuk menjarangkan anak atau sterilisasi sepertirebusan akar Caesalpinia bonduc (Aru). Tujuan penelitian untuk mengetahui pengaruh obatkontrasepsi tradisional air rebusan akar aru terhadap kualitas pematangan sperma epididimis mencit(Mus musculus). Mencit dipilih secara acak untuk mewakili 4 kelompok dosis yaitu : 0,0 (K0), 16,0(K1) 24,0 (K2) dan 32,0 (K3) μl/gram berat badan/hari. Setiap kelompok perlakuan dilakukandengan 5 kali replikasi. Perlakuan diberikan melalui oral dengan menggunakan jarum gavage selama10 hari berturut-turut. Pembedahan dilakukan pada hari pertama setelah perlakuan selesai.Pengamatan kualitas sperma epididimis dengan perhitungan produksi sperma, % sperm motil, %sperma hidup, % sperma abnormal,dan rangking keaktifan sperma. Untuk mengetahui ada atautidaknya perbedaan yang bermakna antar perlakuan dalam kelompok dilakukan uji Anava satu arah,bila terdapat perbedaan bermakna dilanjutkan uji BNJ untuk membandingkan angka rata-rata antarkelompok perlakuan. Pemberian air rebusan akar Caesalpinea bonduc pada mencit menunjukanpengaruh perbedaan secara signifikan pada jumlah (%) sperma abnormal antara dosis 0,0 μl dengan24,0 dan 32,0 μl serta antara 16,0 μl dengan 24,0 dan 32,0 μl; Jumlah (%) sperma hidup terdapatperbedaan antara dosis 0,0 μl dengan 16,0 , 24,0 dan 32,0 μl; Jumlah (%) sperma motil terdapatperbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl; Rangking keaktifan sperma terdapatperbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl. Pemberian rebusan akar aru berpengaruhsecara signifikan terhadap peningkatan persen sperma abnormal, dan penuruan persen sperma hidup,motil dan keaktifan.AbstractIndonesia has many species of plants that have potent of medicine, one of them cold be as antraditional contraception medicine ussually used to limit child or sterilizatiom such as root boiledwater of Caesalpinia bonduc. The aims of this study is to know the effect of root boiled water ofCaesalpinia bonduc as traditional contraception medicine on quality of sperm maturation ofepididymis of mice (Mus musculus). Mice were choosed radomly and doses gouped: 0,0 (K0 ), 16,0(K1) 24,0 (K2) and 32,0 (K3) μl/gram body weight/day. Each of group was replicated 5 times.Treatment were given by oral with using gavage needle for ten days. Surgery was carried out on firstday after completing treatments. Examination of epidymis sperm by counting number of spermproduction, percentage of motil, live, abnormal sperm, and rank of sperm motility. In order to knowthe deferences between control and treated group, was used one way anava analysis and analysedvalue for comparing between teratment group. The treatments of root boiled water of Caesalpiniabonduc have effect significantly on percentage of abnormal sperm between dose of 0,0 μl with 24,0and 32,0 μl, also between 16,0 μl with 24,0 and 32,0 μl. Percentage of live sperem is different betweendose of 0,0 μl with 16,0 , 24,0 and 32,0 μl. Percentage of motile sperm is defferent significantlybetween dose of 0,0 μl with 24,0 and 32,0 μl. Meanwhile percentage of motile rank has differencebetween dose of 0,0 μl with 24,0 and 32,0 μl. The treatment of root boiled water of Caesalpiniabonduc has effect signifiantly on increasing percentange of sperm abnormality, decreasingpercentage of life sperm, motile and rank of motile of mice sperm.



2020 ◽  
Vol 195 (4) ◽  
pp. 2-10
Author(s):  
A. A. Vasiliev ◽  
A. Gorbunov

Abstract. The purpose of the research is to study the influence of the timing and depth of planting potatoes on the photosynthetic productivity, yield and quality of tubers of varieties of different ripening in the forest-steppe zone of the Chelyabinsk region. Research methods. The setting of field experience, analysis and generalization of research results are performed in accordance with classical methods. Results. The optimal time and depth of potato planting in the Chelyabinsk region was established – planting May 12–15 to a depth of 5–6 cm. This combination of agricultural methods provides the greatest photosynthetic potential (Rozara – 3.49 million m2/day, Kuzovok – 4.66 million m2/day), effective assimilation of photosynthetically active radiation (FAR assimilation coefficient: Rozara variety – 3.4 %, body weight – 3.6 %), high yield (Rozara – 35.4 t/ha, body weight – 38.3 t/ha) and the quality of potato tubers (starchiness of Rozara tubers – 16.8 %, Kuzovok – 15.84 %, starch collection per unit area 6.10 and 6.28 t/ha respectively). The late planting period (June 5–12) reduced the photosynthetic potential (Rozara varieties – by 18.7 %, Kuzovok – by 23.6 %) and the FAR coefficient (Rozara – to 2.5–2.6 %, Kuzovok – to 3.0 %). As a result, there was a decrease in the yield of the Rozara variety by 5.1–8.1 t/ha, Kuzovok – by 7.1–7.2 t/ha depending on the depth of planting and a deterioration in the quality of tubers. The dry matter content in late-planted tubers of the Rozara variety decreased by an average of 2.7 %, starch – by 2.29 %, and in the Kuzovok variety – by 2.9 and 1.83 % compared with planting in the second decade of May (accumulation nitrates in tubers increased 2.06 and 2.48 times, respectively). Planting to a depth of 5–6 cm provided an advantage in the second decade of May, and to a depth of 10–12 cm – when planting potatoes in the third decade of May and early June. Scientific novelty. In the conditions of the forest-steppe zone of the Chelyabinsk region, the dependence of the planting depth on the potato planting period is revealed. When planting at the optimum time (May 12–15), the best results are provided by a planting depth of 5–6 cm, while in the third decade of May and early June, the potato planting depth should be increased to 10–12 cm.



2019 ◽  
Vol 99 (6) ◽  
pp. 966-968
Author(s):  
G.A. Amankwa ◽  
S. Mishra ◽  
A.D. Shearer ◽  
C. Saude ◽  
D.L. Van Hooren ◽  
...  

CTH144 is a new flue-cured tobacco (Nicotiana tabacum L.) hybrid recommended for commercial release in Canada. The cured leaf quality of CTH144 was superior to both check varieties Delgold and CT157. The yield potential of CTH144 was intermediate compared with the checks. As a result, the economic return of CTH144 is superior to both check varieties. CTH144 has resistance to Tobacco Mosaic Virus. CTH144 is adapted to the tobacco-growing areas of southwestern Ontario.





2012 ◽  
Vol 49 ◽  
pp. 14-20 ◽  
Author(s):  
M. C. Vann ◽  
L. R. Fisher ◽  
D. L. Jordan ◽  
D. H. Hardy ◽  
W. D. Smith ◽  
...  


2020 ◽  
Vol 12 (4) ◽  
pp. 146
Author(s):  
MAMAT H.S ◽  
S.R.P. SITORUS SITORUS ◽  
H. HARDJOMIDJOJO H. HARDJOMIDJOJO ◽  
A.K. SETA A.K. SETA

ABSTRAK<br />Tembakau Temanggung merupakan komoditas penting bahan baku<br />industri rokok kretek, sumber pendapatan petani dan PAD Kabupaten<br />Temanggung. Selain itu, tembakau juga menjadi pemicu pertumbuhan<br />kegiatan ekonomi lainnya yang terkait dengan usahatani, seperti<br />transportasi, penyediaan sarana produksi pertanian serta penyediaan<br />lapangan kerja. Usahatani tembakau Temanggung menghadapi 3 (tiga)<br />masalah utama, yaitu: sifat tembakau Temanggung yang tergolong fancy<br />product, struktur pasar yang monopsonistik dan kondisi lahan usahatani<br />yang beragam (beragam menurut elevasi, arah lereng dan tingkat<br />kemiringan lereng). Kondisi lahan tersebut mengakibatkan mutu tembakau<br />menjadi beragam, dan cenderung menurun bahkan mengancam keber-<br />lanjutan usahatani tembakau. Penelitian ini dilaksanakan bulan Januari<br />2004 - Maret 2005 di sentra produksi tembakau Temanggung, yang<br />memiliki beberapa perbedaan berdasarkan elevasi, arah lereng dan tingkat<br />kemiringan. Untuk mengetahui ragam mutu dan produktivitas dianalisis<br />dengan sidik ragam. Untuk mengetahui pengaruh elevasi, arah dan<br />kemiringan  lereng  digunakan  analisis  regresi  berganda.  Dalam<br />menganalisis keberlanjutan usahatani, digunakan multi atribut non-<br />parametrik yang diolah dengan multidimentional scaling (MDS). Mutu dan<br />produktivitas tembakau Temanggung cukup beragam. Elevasi dan arah<br />lereng (slope aspect) merupakan faktor utama yang mempengaruhi mutu<br />dan produktivitas tembakau Temanggung. Mutu tembakau yang ditanam<br />pada lahan berelevasi di atas 1.000 m dpl, nyata lebih baik dibandingkan<br />dengan mutu tembakau yang ditanam pada lahan yang berelevasi kurang<br />dari 1.000 m dpl. Produktivitas tembakau yang ditanam pada lahan arah<br />lereng ke timur nyata lebih tinggi dibandingkan dengan produktivitas<br />tembakau pada lahan arah lereng ke timur laut dan utara. Tingkat<br />kemiringan lereng tidak berpengaruh terhadap mutu maupun produktivitas<br />tembakau. Indeks keberlanjutan usahatani tembakau Temanggung,<br />termasuk kedalam kategori cukup (IKb = 55,53 pada skala keberlanjutan 0<br />– 100).<br />Kata kunci : Tembakau, Nicotiana tabacum L., tembakau Temanggung,<br />mutu, produktivitas, indeks keberlanjutan, usahatani, Jawa<br />Tengah<br />ABSTRACT<br />Analysis of quality, productivity and sustainability and<br />development direction of tobacco farming in Temanggung<br />District, Central Java<br />Temanggung tobacco is an important commodity for cigarette<br />industry, farmers’ income and product domestic regional brutto (PDRB)<br />of Temanggung District. Tobacco stimulates economy activities, so that it<br />can grow other bussiness activities, such as transportation, agroproduct<br />and employment availability. Uncontrolled cultivation intensity and<br />market structure monopsonistic resulted in weak bargaining position of<br />farmer in marketing tobacco. Tobacco is a fancy product, it means that its<br />marketing and transaction are very determined by quality. These<br />conditions affected the sustainability of tobacco farm. This research was<br />carried out from January 2004 to March 2005 in the center of Temanggung<br />tobacco production, which varies in terms of elevation, slope aspect and<br />topography. Temanggung tobacco varies in terms of quality and<br />productivity. Elevation and slope aspect were the primary factors<br />influencing tobacco productivity and quality. The quality of tobacco<br />planted at farmer location at the elevation more than 1.000 m above sea<br />level (asl) with slope facing east, was significantly better than the quality<br />of tobacco planted at the elevation less than 1.000 m asl with slope facing<br />north-east and north. The tobacco productivity planted on the slope facing<br />east differed significantly with the tobacco productivity planted on north-<br />east and north slope facing. The slope did not significantly influence<br />tobacco quality and productivity. Sustainability index of tobacco farm<br />belongs to enough category (IKb = 55.53 at scale of sustainability 0 –<br />100).<br />Key words : Nicotiana tabacum L. Temanggung tobacco, quality,<br />productivity, sustainability index, farming system, Central<br />Java



2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Vergina Claudia ◽  
Edwin De Queljoe ◽  
Lydia Tendean

Abstract: Vitamin C is an antioxidant that suppresses the oxidative stress caused by cigarette smoke purpose of this study demonstrate whether vitamin C can provide a different quality of sperm male mice Mus musculus L are given exposure to cigarette smoke. Methodology: The study subjects are 32 male mice Mus musculus L, which were randomly divided into 2 groups. The first group was the control group (K) who received treatment exposure from cigarette smoke without giving vitamin C and the second group is the treatment group (P) are given exposure from cigarette smoke and vitamin C dose of 0.40 mg / gBB /day. The treatment during 30 days, on day 31 the mice were terminated, and then examined the quality of spermatozoa motility of spermatozoa, sperm concentration and morphology of spermatozoa. Results: Test oneway ANOVA on the mean motility of spermatozoa showed Ma control group (47,38%), Mb (11,88%), Mc (18,13%), Md (22,63%) and the Ma treatment group (11.68%), Mb (17,04%), Mc (35,77%), Md (16,5%). The mean concentration of spermatozoa obtained 63,34x105/ml control group and the treatment group and the mean morphology of spermatozoa 81,75x105/ml control group showed normal morphology 50% and abnormal morphology 56,189% while the treatment group showed normal morphology 60% and 40% abnormal. Result from Oneway ANOVA test there are differences significant in the morphology of spermatozoa, concentration of spermatozoa, sperm concentration, and sperm morphology between groups (p <0,05). Conclusion: Vitamin C can improve the quality of spermatozoa after exposure to cigarette smoke. Keywords: ciggaratte, vitamin C, quality of spermatozoa.   Abstrak: Vitamin C merupakan  antioksidan yang menekan proses stres oksidatif akibat asap rokok Tujuan penelitian membuktikan apakah vitamin C dapat memberikan perbedaan kualitas spermatozoa mencit jantan Mus Musculus L yang diberi paparan asap rokok. Metodologi: Subyek penelitian 32 ekor mencit jantan Mus Musculus L, yang dibagi secara acak menjadi 2 kelompok. Kelompok pertama adalah kelompok kontrol (K) yang mendapat perlakuan paparan asap rokok tanpa pemberian vitamin C dan kelompok kedua yaitu kelompok perlakuan (P) yang diberi paparan asap rokok dan vitamin C dosis 0,40mg/gBB/hari. Perlakuan berlangsung selama 30 hari, pada hari ke-31 mencit tersebut diterminasi, kemudian dilakukan pemeriksaan kualitas spermatozoa yaitu motilitas spermatozoa, konsentrasi spermatozoa dan morfologi spermatozoa. Hasil: Uji oneway ANOVA pada rerata motilitas spermatozoa kelompok kontrol menunjukan Ma(47.38%), Mb(11.88%), Mc(18.13%), Md(22.63%) dan kelompok perlakuan Ma(11.68%), Mb(17.04%), Mc(35.77%), Md(16.5%). Rerata konsentrasi spermatozoa kelompok kontrol didapatkan 63.34x105/ml, dan kelompok perlakuan 81.75x105/ml dan rerata morfologi spermatozoa kelompok kontrol menunjukkan morfologi normal 50% dan morfologi abnormal 56,189% sedangkan kelompok perlakuan menunjukkan morfologi normal 60% dan abnormal 40%. Hasil Uji oneway ANOVA terdapat perbedaan yang signifikan pada konsentrasi spermatozoa yaitu morfologi spermatozoa, konsentrasi spermatozoa, dan morfologi spermatozoa antar kelompok (p<0.05). Kesimpulan: Pemberian vitamin C dapat memperbaiki kualitas spermatozoa setelah pemaparan asap rokok. Kata kunci: Rokok, vitamin C, kualitas spermatozoa.



1986 ◽  
Vol 66 (1) ◽  
pp. 193-195
Author(s):  
R. S. PANDEYA

Candel flue-cured tobacco (Nicotiana tabacum L.) with good quality of cured leaves, lower smoke tar:nicotine ratio and early maturing habits was developed at the Agriculture Canada Research Station, Delhi, Ontario in an interspecific hybridization study.Key words: Tobacco, Tar:nicotine ratio, cultivar description



2013 ◽  
Vol 16 ◽  
pp. 53-61 ◽  
Author(s):  
Wei Wu ◽  
Xiao-Ping Tang ◽  
Chao Yang ◽  
Hong-Bin Liu ◽  
Nai-Jia Guo


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