scholarly journals PERBEDAAN KUALITAS SPERMATOZOA MENCIT JANTAN (MUS MUSCULUS L) YANG DIBERIKAN VITAMIN C SETELAH PEMAPARAN ASAP ROKOK

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Vergina Claudia ◽  
Edwin De Queljoe ◽  
Lydia Tendean
Keyword(s):  
Treatment Group ◽  
Vitamin C ◽  
Cigarette Smoke ◽  
Mus Musculus ◽  
Sperm Concentration ◽  
Control Group ◽  
Male Mice ◽  
Normal Morphology ◽  
The Mean

Abstract: Vitamin C is an antioxidant that suppresses the oxidative stress caused by cigarette smoke purpose of this study demonstrate whether vitamin C can provide a different quality of sperm male mice Mus musculus L are given exposure to cigarette smoke. Methodology: The study subjects are 32 male mice Mus musculus L, which were randomly divided into 2 groups. The first group was the control group (K) who received treatment exposure from cigarette smoke without giving vitamin C and the second group is the treatment group (P) are given exposure from cigarette smoke and vitamin C dose of 0.40 mg / gBB /day. The treatment during 30 days, on day 31 the mice were terminated, and then examined the quality of spermatozoa motility of spermatozoa, sperm concentration and morphology of spermatozoa. Results: Test oneway ANOVA on the mean motility of spermatozoa showed Ma control group (47,38%), Mb (11,88%), Mc (18,13%), Md (22,63%) and the Ma treatment group (11.68%), Mb (17,04%), Mc (35,77%), Md (16,5%). The mean concentration of spermatozoa obtained 63,34x105/ml control group and the treatment group and the mean morphology of spermatozoa 81,75x105/ml control group showed normal morphology 50% and abnormal morphology 56,189% while the treatment group showed normal morphology 60% and 40% abnormal. Result from Oneway ANOVA test there are differences significant in the morphology of spermatozoa, concentration of spermatozoa, sperm concentration, and sperm morphology between groups (p <0,05). Conclusion: Vitamin C can improve the quality of spermatozoa after exposure to cigarette smoke. Keywords: ciggaratte, vitamin C, quality of spermatozoa.   Abstrak: Vitamin C merupakan  antioksidan yang menekan proses stres oksidatif akibat asap rokok Tujuan penelitian membuktikan apakah vitamin C dapat memberikan perbedaan kualitas spermatozoa mencit jantan Mus Musculus L yang diberi paparan asap rokok. Metodologi: Subyek penelitian 32 ekor mencit jantan Mus Musculus L, yang dibagi secara acak menjadi 2 kelompok. Kelompok pertama adalah kelompok kontrol (K) yang mendapat perlakuan paparan asap rokok tanpa pemberian vitamin C dan kelompok kedua yaitu kelompok perlakuan (P) yang diberi paparan asap rokok dan vitamin C dosis 0,40mg/gBB/hari. Perlakuan berlangsung selama 30 hari, pada hari ke-31 mencit tersebut diterminasi, kemudian dilakukan pemeriksaan kualitas spermatozoa yaitu motilitas spermatozoa, konsentrasi spermatozoa dan morfologi spermatozoa. Hasil: Uji oneway ANOVA pada rerata motilitas spermatozoa kelompok kontrol menunjukan Ma(47.38%), Mb(11.88%), Mc(18.13%), Md(22.63%) dan kelompok perlakuan Ma(11.68%), Mb(17.04%), Mc(35.77%), Md(16.5%). Rerata konsentrasi spermatozoa kelompok kontrol didapatkan 63.34x105/ml, dan kelompok perlakuan 81.75x105/ml dan rerata morfologi spermatozoa kelompok kontrol menunjukkan morfologi normal 50% dan morfologi abnormal 56,189% sedangkan kelompok perlakuan menunjukkan morfologi normal 60% dan abnormal 40%. Hasil Uji oneway ANOVA terdapat perbedaan yang signifikan pada konsentrasi spermatozoa yaitu morfologi spermatozoa, konsentrasi spermatozoa, dan morfologi spermatozoa antar kelompok (p<0.05). Kesimpulan: Pemberian vitamin C dapat memperbaiki kualitas spermatozoa setelah pemaparan asap rokok. Kata kunci: Rokok, vitamin C, kualitas spermatozoa.

scholarly journals Perubahan Kualitas Sperma Mencit (Mus musculus) Yang Terpapar Asap Rokok Elektrik

2019 ◽  
Vol 3 (2) ◽  
pp. 122-128
Author(s):  
Andita Ayu Mandasari ◽  
Siti Nur Asiyah ◽  
Kurnia Lintang
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Mus Musculus ◽  
Group Treatment ◽  
Time Lag ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
Male Mice ◽  
Suction Device ◽  
Treatment Groups

Smoking is one of the habits that can affect human health. One type of cigarette that is currently the choice is electric cigarettes. The e-cigarette content includes nicotine, propylene glycol, flavorings and glycerin. The purpose of this study was to determine the effect of exposure to e-cigarette smoke on mice strain balb / c with the parameters of the number of spermatozoa. This study used 15 male mice which were 8-10 weeks old and weighed 25-30 grams of male mice which were divided into 3 treatment groups namely control group, treatment group one and treatment group two. In the control group no exposure to cigarette smoke was carried out while in the treatment group one was exposed to cigarette smoke in mice with a dose of 0 mg nicotine and the second treatment group was exposed to e-cigarette smoke at a dose of 18 mg nicotine. Electric cigarette smoke exposure is carried out for 30 days using a suction device from a syringe. The exposure of cigarette smoke is carried out every day with successive doses of 20 times as much as 3 times with a time lag of 10 minutes (5 minutes the chamber is closed and 5 minutes of the chamber is opened). The results showed that the average number of spermatozoa in the control group was 16.816 million / ml. The average number of spermatozoa in treatment one was 10.432 million / ml and the average number of spermatozoa in treatment two was 5.234 million / ml. This shows that exposure to cigarette smoke can reduce the number of spermatozoa from the control group to the first treatment by 42.56% and the second treatment shows a decrease from the first group by 34.65%.

scholarly journals Noise Exposure Decreases the Number of Spermatozoa in Male Mice (Mus musculus L.) Even With Grape Seed Extract (Vitis vinifera L.) Administration

2020 ◽  
Vol 5 (2) ◽  
pp. 70-75
Author(s):  
Ghestiara Siregar
Keyword(s):  
Treatment Group ◽  
Mus Musculus ◽  
Noise Exposure ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Control Group ◽  
Vitis Vinifera L ◽  
Not Given ◽  
Male Mice

  Excessive continuous noises exposure changes the male hormone system which leads to formation of oxidative stress and results in disrupt of semen quality. This condition can be reduced by the use of antioxidants. Grape seed is one of the antioxidants that contains phenol components that have Resveratrol compounds. This research aimed to observe the effect of noise exposure on the number of spermatozoa of male mice given grape seed extract. The method of the research was a post-test only control group design with research subjects of 30 male Mus musculus L. (Swiss Webster) mice divided into 5 groups: group A (treatment control) was not given noise exposure and grape seed extract, group B (negative control) was not given noise exposure and was given grape seed extract, groups C, D, E (treatment group) were given noise exposure with sequential intensities of 65 dB, 85 dB, 105 dB. The treatment was given for 33 days. The results showed that noise exposure with different intensities of 65 dB, 85 dB, 105 dB reduced the spermatozoa count of male Swiss Webster mice even with the administration of grape seed extract. One Way Anova test was used to analyze the data with p-value of 0.001. Conclusion: There were differences in spermatozoa count between the control group and the treatment group. Provision of noise exposure with a value above the threshold limit reduces the number of male Swiss Webster mice spermatozoa given with grape seed extract. Keywords: Number of Spermatozoa, Grape Seed Extract, Noise exposure  

scholarly journals PENGARUH PAPARAN ASAP ROKOK KRETEK TERHADAP KUALITAS SPERMATOZOA MENCIT JANTAN (MUS MUSCULUS)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Immanuel Van Donn Batubara ◽  
Benny Wantouw ◽  
Lydia Tendean
Keyword(s):  
Cigarette Smoke ◽  
Mus Musculus ◽  
Sperm Quality ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
Not Given ◽  
Significant Difference ◽  
Key Word ◽  
Randomized Experimental Design

Abstrak: Asap rokok mengandung tiga komponen toksik utama, yaitu karbonmonoksida, nikotin, dan tar yang dapat menyebabkan gangguan pada spermatogenesis. Tujuan penelitian ini adalah untuk melihat pengaruh paparan asap rokok kretek terhadap kualitas spermatozoa mencit. Penelitian ini adalah penelitian eksperimental dengan rancangan acak lengkap. Subyek penelitian sebanyak 25 ekor mencit jantan (Mus musculus) yang dibagi secara acak menjadi 5 kelompok: kelompok P0 tidak diberikan paparan asap rokok, kelompok P1 diberikan paparan asap rokok kretek 1 batang, kelompok P2 diberikan paparan asap rokok kretek 2 batang, kelompok P3 diberikan paparan asap rokok kretek 3 batang, kelompok P4 diberikan paparan asap rokok kretek 4 batang. Perlakuan diberikan selama 30 hari. Hasil penelitian menunjukkan adanya perbedaan yang signifikan pada konsentrasi dan motilitas spermatozoa kelompok yang mendapatkan paparan asap rokok kretek (p<0,05) dibandingkan dengan kelompok kontrol. Morfologi spermatozoa kelompok P2,P3,P4 didapati hasil yang berbeda signifikan dengan kelompok kontrol (p<0,05) tetapi kelompok P1 hasilnya berbeda tak signifikan dengan kelompok kontrol. Paparan asap rokok kretek memberikan pengaruh negatif terhadap kualitas spermatozoa yang disebabkan oleh adanya radikal bebas yang dihasilkan oleh asap rokok. Kata Kunci: asap rokok, kualitas spermatozoa.     Abstract: Cigarette smoke contains three main toxic components, namely carbon monoxide, nicotine, and tar that can cause disturbances in spermatogenesis. The objective of this study was to investigate and observe the effect of cigarette smoke exposure on the quality of spermatozoa of mice. The study was a completely randomized experimental design. The research subject as many as 25 male mice who were randomly divided into 5 groups: group P0 not given exposure, the P1 is given exposure to smoke from 1 bar of cigarette, the P2 is given exposure to smoke from 2 bars of cigarette, the P3 is given exposure to smoke from 3 bars of cigarette and finally the P4 is given exposure to smoke from 4 bars of cigarette. The treatment is given for 30 days. At the end of experiment, results showed a significant difference in the concentration and motility of spermatozoa between the groups exposed to cigarette smoke (p<0,05) compared with the control group. The morphology of spermatozoa group P2,P3,P4, was found substantially different with the control group (p<0,05) but group P1 did not have significant difference in results with the control group. The exposure to cigarette smoke negatively affects sperm quality caused by the free radicals generated by the smoke. Key Word: cigarette smoke, sperm quality.

PENURUNAN LAJU PENUAAN REPRODUKSI MENCIT JANTAN (Mus muculus.Linn) DENGAN PEMBERIAN EKSTRAK JAHE (Zingiber officinale) DALAM PAKAN

2018 ◽  
Vol 5 (1) ◽  
pp. 1-10
Author(s):  
Sutyarso Sutyarso ◽  
Sumayyah Annida ◽  
M. Kanedi ◽  
Hendri Busman ◽  
Nuning Nurcahyani
Keyword(s):  
Mus Musculus ◽  
Reproductive Function ◽  
Zingiber Officinale ◽  
Male Mice ◽  
Ginger Extract ◽  
Normal Morphology ◽  
Aging Period ◽  
Ginger Rhizome ◽  
Rate Of Decline

The ginger rhizome contains antioxidants and is chemoprotective, therefore we suspect it can decrease the aging rate in the reproductive system. The purpose of this study, to determine the effect of ginger extract (Zingiber officinale) against reproductive aging of male mice (Mus musculus). This study, using 36 male mice (Mus musculus) aged 12-14 months, divided into three groups each 12 tails. Group 1 as controls, groups 2 and 3 were given 50 mg and 100 mg ginger extract / kg of pellets. The pellet is given for 70 days ad libitum. Next, observed the number of spermatogenic cells, as well as the number and quality of spermatozoa. The results showed that ginger extract had an effect on spermatogenic and spermatozoa (P <0,01) cells of mice. The results of preleptotene spermatocyte, pakhiten and spermatid spermatocyte counts were higher, as were the number of spermatozoa, the percentage of viability and motility, and the normal morphology of spermatozoa were more in the group given ginger extract than control (P <0.01). It was concluded that ginger rhizome extract given to mice entering the aging period could inhibit the rate of decline in reproductive function.

scholarly journals The Effect of Antioxidant Vitamin C and E Administration on Spermatozoa Quality of White Rat (Rattus Norvegicus) Strain Wistar Male Against Allethrin Exposure

2018 ◽  
Vol 1 (2) ◽  
pp. 113
Author(s):  
Faisal Yusuf Ashari ◽  
Rina Yudiwati ◽  
R.Haryanto Aswin
Keyword(s):  
Vitamin C ◽  
Rattus Norvegicus ◽  
The Body ◽  
Control Group ◽  
Antioxidant Vitamin ◽  
Control Group Design ◽  
Spermatozoa Motility ◽  
Treatment Groups ◽  
The Mean

Introduction: Allethrin is an active ingredient used in anti-mosquito drugs. Allethrin enters the body through inhalation, allegedly can disrupt the quality of spermatozoa by decreasing its amount, motility, viability and morphology. Many studies suggested that with sufficient intake of vitamin C and E would be able to minimize free radicals caused by allethrin. This study aims to investigate the effect of giving antioxidant vitamin C and E on spermatozoa quality of white rat (Rattus norvegicus) strain Wistar male with allethrin exposure.Methods: This research was a post-test design randomized control group design using Rattus novergicus male strains Wistar exposed to allethrin (LPB IPB veterinary stem cell). A total of 16 white rat strains Wistar male divided into 4 groups, one control group (without any treatment) and 3 treatment groups (given antioxidant vitamin C, vitamin E and both). The exposure of mosquito coils was performed 8 hours per day for 45 days and on the 46th day, spermatozoa was observed. The data were analyzed using ANOVA one way and BNT.Results: The results showed that treatment with vitamins affected the amount, motility and viability of rat spermatozoa exposed to allethrin. The mean amount of control group spermatozoa and treatment was 14,93, the mean spermatozoa motility control and treatment groups were 45,13 and the mean spermatozoa viability of control and treatment groups was 58,88. This is shown by the real difference between the control group and the treatment (p <0,05). Similarly, in the control group found morphological abnormalities.Conclusion: Exposure of mosquito coils 8 hours/day for 45 days decreased the spermatozoa quality. Overall, the admission of antioxidant vitamin C and E are able to maintain the quality of spermatozoa instead of one type of vitamin.

scholarly journals PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK

2020 ◽  
Vol 9 (1) ◽  
pp. 7
Author(s):  
Winny Jeanita ◽  
Dewa Ketut Meles ◽  
Widjiati WIdjiati ◽  
Iwan Sahrial Hamid ◽  
Epy Muhammad Luqman ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Mus Musculus ◽  
Pulmonary Veins ◽  
Bronchial Epithelium ◽  
Musa Acuminata ◽  
Banana Peel ◽  
Control Group ◽  
Male Mice ◽  
Post Hoc ◽  
Peel Extract

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.

scholarly journals PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP PENURUNAN KADAR MALONDIALDEHID (MDA) PARU MENCIT JANTAN (Mus musculus) YANG DIPAPAR ASAP ROKOK

2020 ◽  
Vol 8 (2) ◽  
pp. 123
Author(s):  
Sapna Kurnia Dewi ◽  
Widya Paramitha Lokapirnasari ◽  
Dewa Ketut Meles ◽  
Imam Mustofa ◽  
Maslichah Mafruchati ◽  
...  
Keyword(s):  
Vitamin C ◽  
Cigarette Smoke ◽  
Mus Musculus ◽  
Musa Acuminata ◽  
Banana Peel ◽  
Male Mice ◽  
Significant Difference ◽  
Per Oral ◽  
One Way Anova ◽  
Peel Extract

This research aimed to determine the effect of kepok banana peel extract (Musa acuminata) given by per oral in decreasing levels of malondialdehyde (MDA) in lung of male mice (Mus musculus) which was exposed to cigarette smoke. The 24 experimental male mice were divided into 6 groups. The therapy was given for 14 days, all mice were sacrificed and lung MDA level were examined with thiobarbituricacid (TBA) methods. The result of the data analysis test using one-way ANOVA (Analysis of Variance) showed that there were significant differences between the group of animals that exposed to cigarette smoke ( = 840.50± 186.47 nmol / g) and the group of animals that were given with extract of kepok banana peel 56 mg / kg bw  ( = 6471.25 ± 69.25 nmol/g). Besides that, there was an significant difference between the groups of animals that were exposed cigarette smoke ( = 840.50± 186.47 nmol / g) with the group of animals that were given with vitamin C 260 mg/kg bw ( = 555.25± 61.16 nmol / g). It could be concluded that extract of kepok banana peel and vitamin C could decrease MDA levels in lung of male mice which was exposed cigarette smoke. The increased therapy dose gave affect in lowering levels of malondialdehyde in lung.

scholarly journals Kebar grass (Biophytum petersianum K.) The effect inmaintaining mice (Mus musculus) sperm quality exposed to dioxin

2020 ◽  
Vol 11 (3) ◽  
pp. 4977-4981
Author(s):  
Muhammad Faiz Labib ◽  
Widjiati ◽  
Tatik Hernawati ◽  
Epy Muhammad Luqman ◽  
Rochmah Kurnijasanti ◽  
...  
Keyword(s):  
Treatment Group ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Experimental Laboratory ◽  
Tetrachlorodibenzo P Dioxin

Kebar grass contains active antioxidants and potential vitamins to neutralize TCDD toxicity. Prove that Kebar grass extract in various dosage can maintain viability, motility, and sperm concentration of male mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. This study was an experimental laboratory study with five groups consists of Negative Control (C-), Positive Control (K +) with TCDD exposure of 0.7μg/Kg BW IP single dose. The treatment group was given oral Kebar grass extract for 53 days treatment 1 (T1) with a dose of 0.045 mg / g BB, Treatment 2 (T2) with a dose of 0.08 mg/g/BW, and Treatment 3 (T3) with a dose of 0.135 mg/g/BW. The data of motility, viability, and concentration of spermatozoa obtained were analyzed using One Way Anova test and Duncan Multiple Range Test. Administration of Kebar Grass Extract at a dosage of 0.135 mg/g/BW showed a significant difference between the control group and the treatment group (p&lt;0.05). Exposure to TCDD in C+ decreased motility (13 ± 6.70%), viability (28 ± 19.35%), and concentration (0.87 ± 0.64 cells / mm3) of sperm significantly compared to C-. The administration of Kebar grass extract can maintain motility (74 ± 5.47%), viability (76 ± 2.72%), and concentration (2.50 ± 0.69 cells/mm3) spermatozoa in the T3 group with a dose of 0.135mg/g/BW. Kebar grass extract is effective for maintaining the quality of mice sperm from damage due to exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin.

scholarly journals PERBEDAAN ANTARA EFEK PEMBERIAN VITAMIN C DAN VITAMIN E TERHADAP KUALITAS SPERMATOZOA TIKUS WISTAR (RATTUS NORVEGICUS) JANTAN SETELAH DIBERI PAPARAN ASAP ROKOK

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Astrid Giovani Sitohang ◽  
Benny Wantouw ◽  
Edwin De Queljoe
Keyword(s):  
Free Radicals ◽  
Vitamin E ◽  
Vitamin C ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Water Soluble ◽  
Male Wistar Rats ◽  
The Mean

Abstract: Gaseous components of cigarette smoke have a potential to generate free radicals that can damage sperm. Vitamin C is water soluble and vitamin E is a fat-soluble antioxidant that is effective in dealing with free radicals. The aim of this research was to look at the differences between the effects of vitamin C and vitamin E on spermatozoa quality of male wistar rats (Rattus norvegicus) after exposure to cigarette smoke. Methods: This research used a completely randomized experimental design. The sample of the research were 15 male wistar rats which were randomly divided into 3 groups, i.e. group P0 was given exposure to smoke from 2 bars of cigarette without giving vitamin C and vitamin E, group P1 was given exposure to smoke from 2 bars of cigarette and vitamin C 1,8 mg / day and group P2 was given exposure to smoke from 2 bars of cigarette and vitamin E 1,44 mg / day. The treatment was given for 52 days. Results: The results of this research showed an increasing spermatozoa quality of group P1 and P2 compared with the group P0. The mean concentration of spermatozoa obtained on group P1 (78,78x106/ml) and group P2 (123,13 x106/ml). The mean motility of spermatozoa obtained on group P1 (72,00%) and group P2 (80,25%). The mean of normal morphology of spermatozoa obtained on group P1 (67,63%) and group P2 (74,00%). Conclusion: The conclusion from this research showed that there is a difference between spermatozoa quality of group treated with vitamin C and vitamin E after exposure to cigarette smoke, with vitamin E had an higher average on spermatozoa quality.Keywords: cigarette smoke, vitamin C, vitamin E, the quality of spermatozoaAbstrak: Latar Belakang: Komponen gas dari asap rokok berpotensi untuk menimbulkan radikal bebas yang dapat merusak spermatozoa. Vitamin C yang larut dalam air dan vitamin E yang larut dalam lemak merupakan antioksidan yang efektif dalam mengatasi radikal bebas. Tujuan penelitian ini adalah untuk melihat perbedaan efek antara pemberian vitamin C dan vitamin E terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) jantan setelah diberi paparan asap rokok. Metode: Penelitian ini menggunakan metode penelitian eksperimental dengan menggunakan rancangan acak lengkap. Sampel yang digunakan adalah 15 tikus wistar jantan yang dibagi secara acak menjadi 3 kelompok yaitu kelompok P0 yang mendapat perlakuan paparan asap rokok 2 batang tanpa pemberian vitamin C dan vitamin E, kelompok P1 dengan perlakuan pemaparan asap rokok 2 batang dan vitamin C 1,8 mg/hari dan kelompok P2 dengan perlakuan pemaparan asap rokok 2 batang dan vitamin E 1,44 mg/hari. Perlakuan diberikan selama 52 hari. Hasil: Hasil penelitian menunjukkan adanya peningkatan kualitas spermatozoa kelompok P1 dan P2 dibandingkan dengan kelompok P0. Rata-rata konsentrasi spermatozoa kelompok P1 didapatkan (78,78x106/ml) dan kelompok P2 (123,13 x106/ml). Rata-rata motilitas spermatozoa kelompok P1 (72,00%) dan kelompok P2 (80,25%). Rata-rata morfologi normal spermatozoa kelompok P1 (67,63%) dan kelompok P2 (74,00%). Simpulan: Kesimpulan dari penelitian ini didapatkan bahwa terjadi perbedaan kualitas spermatozoa antara pemberian vitamin C dan vitamin E setelah pemaparan asap rokok, dengan vitamin E memiliki rata-rata kualitas spermatozoa lebih tinggi.Kata kunci: asap rokok, vitamin C, vitamin E, kualitas spermatozoa

scholarly journals Morel berry leaf extract reduce levels of reactive oxygen species and increase levels of endothelial nitric oxide synthase of male Wistar rat exposed to cigarette smoke

2021 ◽  
Vol 4 (1) ◽  
pp. 28-31
Author(s):  
Felicia Hartono ◽  
Gde Ngurah Indraguna Pinatih ◽  
Anak Agung Ayu Ngurah Susraini
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Endothelial Nitric Oxide Synthase ◽  
Leaf Extract ◽  
Control Group ◽  
Oxygen Species ◽  
Male Wistar Rats ◽  
The Mean ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Background: Exposure to cigarette smoke increased the level of reactive oxygen species (ROS) and caused oxidative stress which could lead to early aging includes vascular aging. Morel berry leaf contained flavonoid works as antioxidant that neutralized ROS. This study aimed to investigate effectivenes of Morel berry (Physalis angulata L.) leaf extract to decrease levels of ROS and increase levels of endothelial nitric oxide synthase (eNOS) in male Wistar rats exposed to cigarette smoke. Method: This research was an experimental study with pre test-post test control group design. 16 healthy male rats (Rattus norvegicus), Wistar strain, 3-4 months, weight 180-200gr were used, divided randomly into two groups. The control group was given aquadest and the treatment group was given Morel berry leaf extract 500 mg/kgBW/day. Continued to 30 minutes exposed to cigarette smoke. ROS and eNOS levels were measured before and after 28 days treatment, using rat ROS ELISA Kit and rat ENOS ELISA Kit respectively. Results: The results showed that the mean ROS levels in the treatment group decreased significantly and was significantly lower than in control group (75.789 + 3.298 vs 91.646 + 1.970; p < 0.001). The mean eNOS levels in the treatment group increased significantly and was significantly higher than control group (8.597 + 0.375 vs 5.957 + 0.329; p < 0.001). Conclusion: The results of this study indicated that administration of Morel berry leaf extract reduced levels of ROS and increased serum levels of eNOS in male Wistar rats exposed to cigarette smoke.

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