scholarly journals Insights into low biological activity of wax apple (Syzygium samarangense) juice by in vitro phytochemical investigation with special reference to metabolomics

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Soumya Majumder ◽  
SUKANYA ACHARYYA ◽  
ARINDAM GHOSH ◽  
SOURAV CHAKRABORTY ◽  
SAHADEB SARKAR ◽  
...  
Keyword(s):  
Biological Activity ◽  
Special Reference ◽  
Cardiac Glycosides ◽  
Fruit Juice ◽  
Biological Activities ◽  
Bioactive Components ◽  
Biochemical Tests ◽  
Phytochemical Investigation ◽  
Qualitative Detection

Abstract. Majumder S, Acharyya S, Ghosh A, Chakraborty S, Sarkar S, Saha S, Bhattacharya M. 2021. Insights into low biological activity of wax apple (Syzygium samarangense) juice by in vitro phytochemical investigation with special reference to metabolomics. Biofarmasi J Nat Prod Biochem 19: 30-38. Wax-apple (Syzygium samarangense) is an Asian waxy and juicy fruit which is reported as antidiarrheal and useful in diseases like dysentery. Moreover, no stronger medicinal evidences have been found associated with this fruit as well as its juice to ensure more biological activities and composition. Being edible, waxy and juicy this fruit is quite popular in coastal areas and islands but, absence of stronger medicinal and biochemical evidences associated with this fruit or its juice has created an worldwide underutilizing status on it. In this research, we intended to investigate phytochemical characteristics and composition of this fruit juice through detailed biochemical tests and metabolomics. Qualitative detection tests for bioactive groups of molecules (tannin, coumarin, cardiac glycosides, terpenoids, flavonoids, phenol etc.), antioxidant assay and antibacterial test simply showed its low in vitro biological activity. A GC-MS based metabolomics was performed where presence of wax components like long chain hydrocarbons have been revealed. Based on the GC-MS based metabolomics a proposed pathway of wax apple’s wax biosynthesis has been established. The overall study not only strongly clarified the absence of bioactive components in this fruit but also proved that the fruit, named wax apple, is actually a source of natural waxes which can be responsible for its claimed antidiarrheal property.

Design, synthesis and biological activities of pyrrole-3-carboxamide derivatives as EZH2 (enhancer of zeste homologue 2) inhibitors and anticancer agents

2020 ◽  
Vol 44 (6) ◽  
pp. 2247-2255
Author(s):  
Qifan Zhou ◽  
Lina Jia ◽  
Fangyu Du ◽  
Xiaoyu Dong ◽  
Wanyu Sun ◽  
...  
Keyword(s):  
Biological Activity ◽  
Anticancer Agents ◽  
Biological Activities ◽  
Activity Assay ◽  
Design Synthesis ◽  
Structure Activity Relationships ◽  
Structure Activity ◽  
Enhancer Of Zeste

A novel series of pyrrole-3-carboxamides targeting EZH2 have been designed and synthesized. The structure–activity relationships were summarized by combining with in vitro biological activity assay and docking results.

scholarly journals Biological activity of the red alga Laurencia brandenii

2011 ◽  
Vol 70 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Aseer Manilal ◽  
Sugathan Sujith ◽  
Balu Sabarathnam ◽  
George Kiran ◽  
Joseph Selvin ◽  
...  
Keyword(s):  
Biological Activity ◽  
Biological Activities ◽  
Sitophilus Oryzae ◽  
Red Alga ◽  
Culture Collection ◽  
Gas Chromatography Mass Spectrometry ◽  
Human Pathogens ◽  
Active Fraction ◽  
Renewable Natural Resource

Biological activity of the red algaLaurencia brandeniiThe marine red algaLaurencia brandeniicollected from the southwest coast of India (Indian Ocean) was extracted and fractioned using column chromatography. The individual fractions were evaluatedin vitrovia antimicrobial activity against six species of Microbial Type Culture Collection and three species of clinical human pathogens, antipest activity onSitophilus oryzae, maggoticidal activity against 2ndinstar larvae ofSarcophagasp. and termiticidal activity againstMicrotermes obesi.It was found that the fraction eluted using petroleum ether:chloroform (6:4) exhibited broader biological activities. The phyco-constituents of the active fraction were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The GC-MS profile of the active fraction revealed that the main constituent was octadecadienoic acid (49.75%) followed by n-hexadecanoic acid (14.24%), which might have a functional role in the biological activities. The overall activity profile envisages that these bioactive compounds fromL. brandeniicould be utilized as a renewable natural resource for the development of novel environmental-compatible formulations for the control of human pathogens, pests, termites and maggots.

scholarly journals Phytochemical composition and in vitro biological activities of Morinda citrifolia fruit juice

2020 ◽  
Author(s):  
Haziz Sina ◽  
Gado Dramane ◽  
Philippe Tchekounou ◽  
Mahoudo Fidèle Assogba ◽  
Kamirou Chabi-Sika ◽  
...  
Keyword(s):  
Fruit Juice ◽  
Biological Activities ◽  
Morinda Citrifolia ◽  
Phytochemical Composition

scholarly journals Biological activities of recombinant chicken leptin C4S analog compared with unmodified leptins

2000 ◽  
Vol 279 (1) ◽  
pp. E116-E123 ◽  
Author(s):  
S. Dridi ◽  
N. Raver ◽  
E. E. Gussakovsky ◽  
M. Derouet ◽  
M. Picard ◽  
...  
Keyword(s):  
Biological Activity ◽  
Food Intake ◽  
Leptin Receptor ◽  
Biological Activities ◽  
Wild Type ◽  
Escherichia Coli Cells ◽  
Prokaryotic Expression Vector ◽  
Long Form

The chicken leptin sequence, in contrast to mammalian leptins, contains an unpaired Cys at position 3 of the original cDNA ( AF012727 ). The presence of an extra Cys may confer a different structure and affect the leptin's biological activity. To address this, we studied the effects of wild-type and mutated (C4S) chicken leptins in vitro and in vivo and compared them with mammalian leptin prepared from ovine leptin cDNA. The prokaryotic expression vector pMON, encoding full-size A(−1) chicken leptin ( AF012727 ), was mutated using a mutagenesis kit, yielding the C4S analog. Escherichia coli cells transformed with this vector overexpressed large amounts of chicken leptin C4S upon induction with nalidixic acid. The expressed protein, found in the inclusion bodies, was refolded and purified to homogeneity on a Q-Sepharose column, yielding three electrophoretically pure fractions, eluted from the column by 100, 125, and 150 mM NaCl, respectively. All three fractions showed a single band of the expected molecular mass (16 kDa) and were composed of >95% monomeric protein. Proper refolding was evidenced by comparing the circular dichroism spectrum of the analog with spectra of nonmutated chicken and ovine leptins. The biological activity of the C4S analog was evidenced by its ability to stimulate proliferation of leptin-sensitive BAF/3 cells transfected with a long form of human leptin receptor construct similar to its nonmutated counterpart, indicating that Cys4 plays no role in leptin activity. The in vitro activity of both wild-type and mutated chicken leptins was ∼10-fold lower than that of ovine leptin. After intravenous or intraperitoneal injections, C4S analog and the nonmutated chicken and ovine leptins all lowered the food intake of starved 9-day-old broiler or 5-wk-old layer male chickens by 11–34%. Monitoring food behavior revealed that the attenuated food intake resulted not from a decreased number of approaches to the feeders but from a decrease in the average time spent eating during each approach.

Biological characterization of charge isomers of human growth hormone

1988 ◽  
Vol 118 (1) ◽  
pp. 14-21 ◽  
Author(s):  
A. Skottner ◽  
A. Forsman ◽  
B. Skoog ◽  
J. L. Kostyo ◽  
C. M. Cameron ◽  
...  
Keyword(s):  
Biological Activity ◽  
Biological Activities ◽  
Human Growth ◽  
Proteolytic Processing ◽  
Biological Characterization ◽  
Growth Promoting ◽  
Rat Adipose Tissue ◽  
Insulin Like Activity

Abstract. Since deamidation of the human GH molecule may alter the manner and extent to which the hormone is cleaved by proteases, and since it has been repeatedly suggested that proteolytic processing is required for the expression of certain of the activities of GH, the present study was conducted to determine whether the biological activity profiles of more acidic forms of human GH are altered. Three charge isomers, GH-b, GH-c and GH-d, representing primarily deamidated forms, were isolated from a native human GH preparation (Crescormon®) in amounts adequate for characterization of their biological activities. All three were essentially equipotent in a radioimmunoassay for human GH. When assessed for growth-promoting activity in the hypophysectomized rat, the isomers were again equipotent with each other and with the GH preparation from which they were derived. The charge isomers also had significant in vitro insulin-like activity on isolated rat adipose tissue and diabetogenic activity in the ob/ob mouse. Thus, the biological activity profiles of these charge isomers of human GH do not differ greatly from one another.

scholarly journals Coordination Compounds of Platinum and Palladium with Mixed Ligands (Usnic Acid and 1-(o-Tolyl) Biguanide) � Synthesis, Spectral Characterization and Biological Activity

2020 ◽  
Vol 71 (4) ◽  
pp. 336-346
Author(s):  
Mirela Calinescu ◽  
Ovidiu Oprea ◽  
Catalina Stoica ◽  
Mihai Nita-Lazar ◽  
Madalina Mihalache
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Biological Activity ◽  
Coordination Compounds ◽  
Antitumor Agents ◽  
Biological Activities ◽  
Usnic Acid ◽  
Biological Properties ◽  
Mixed Ligands

Four coordination compounds of Pd(II), Pt(II) and Pt(IV) with usnic acid (H3AU) and 1-(o-tolyl)biguanide (TB) as ligands have been synthesized in view of their potential as antimicrobial, antifungal and antitumor agents. The metal complexes have been characterized by elemental and thermogravimetrical analyses, infrared and electronic spectra. Based on these studies, the following formulas have been proposed for the complexes: [Pd(TB)(H3AU)]PdCl4 (C1), [Pd(TB)(H2AU)] CH3COO (C2), [Pt(TB)(H2AU)Cl2]Cl (C3) and [Pt(TB)(H2AU)]Cl (C4), where H2AU is deprotonated usnic acid. The in vitro biological activities of the new complexes were tested against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 10231 and HeLa tumor cells. All complexes were found to have good biological properties and therefore they can be further explored in therapeutic applications.

scholarly journals Exploiting the Anti-inflammatory Potential of White Capsicum Extract by the Nanoformulation in Phospholipid Vesicles

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1683
Author(s):  
Ilaria Pappalardo ◽  
Anna Santarsiero ◽  
Maria De Luca ◽  
Maria Assunta Acquavia ◽  
Simona Todisco ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Biological Activities ◽  
Phospholipid Vesicles ◽  
Antioxidant Compounds ◽  
Bioactive Components ◽  
Property A ◽  
Phytochemical Content ◽  
Capsicum Species ◽  
Anti Inflammatory

The peppers of the Capsicum species are exploited in many fields, as flavoring agents in food industry, or as decorative and therapeutic plants. Peppers show a diversified phytochemical content responsible for different biological activities. Synergic activity exerted by high levels of antioxidant compounds is responsible for their important anti-inflammatory property. A methanolic extract was obtained from a new pepper genotype and tested for anti-inflammatory activity. The extract was incorporated into phospholipid vesicles to increase the bioavailability of its bioactive components. Two types of phospholipid vesicles were produced, conventional liposomes and Penetration Enhancer containing Vesicles (PEVs). They were tested in human monoblastic leukemia U937 cell line, showing no cytotoxic effect. The intracellular reactive oxygen species (ROS) and nitric oxide (NO) levels were measured to value the in vitro efficacy of the vesicles in regulating inflammatory responses. Liposomal incorporation significantly reduced ROS levels in extract-treated LPS-activated cells. Furthermore, LC-MS/MS analyses demonstrated that liposomes facilitated the transport of the extract components across the cell membrane and their accumulation into the cytoplasm.

scholarly journals Effect of the Plastein Reaction in Presence of Extrinsic Amino Acids on the Protective Activity of Casein Hydrolysate against Ethanol-Induced Damage in HHL-5 Cells

Foods ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 112
Author(s):  
Li-Ying Bo ◽  
Jia-Nan Pang ◽  
Chun-Li Song ◽  
Tie-Jing Li
Keyword(s):  
Amino Acids ◽  
Biological Activity ◽  
Treatment Time ◽  
Biological Activities ◽  
Casein Hydrolysate ◽  
Protective Activity ◽  
Potential Health ◽  
Plastein Reaction ◽  
Casein Hydrolysates

Casein hydrolysates (CH) were prepared using papain and modified by the plastein reaction (CH-P) in the presence of extrinsic phenylalanine (CH-P-Phe) or tryptophan (CH-P-Trp). The in vitro protective activity of CH and its modified products against ethanol-induced damage in HHL-5 cells was investigated. The results showed that the modification by the plastein reaction reduced the amino group content of CH. However, the modification by the plastein reaction in the presence of extrinsic amino acids could enhance the antioxidant, proliferative, cell cycle arresting, and anti-apoptosis activity of CH. Biological activities of CH and its modified products in the HHL-5 cells varied depending on the hydrolysate concentration (1, 2, and 3 mg/mL) and treatment time (24, 48, and 72 h). Generally, higher biological activities were found after cell treatment with CH or its modified products at concentration of 2 mg/mL for 48 h compared to other treatments. In addition, CH modified in the presence of tryptophan (CH-P-Trp) showed higher biological activity than that modified in the presence of phenylalanine (CH-P-Phe). Based on the obtained results, it can be concluded that casein hydrolysates with enhanced biological activity and potential health benefits can be produced by papain and the plastein reaction with the incorporation of extrinsic amino acids.

Comparative study on the bioactive components and in vitro biological activities of three green seedlings

2020 ◽  
Vol 321 ◽  
pp. 126716
Author(s):  
Xue Li ◽  
Pei Wang ◽  
Jiaqing Zhu ◽  
Juanjuan Yi ◽  
Zhenyu Ji ◽  
...  
Keyword(s):  
Comparative Study ◽  
Biological Activities ◽  
Bioactive Components

scholarly journals In vitro anticancer activity of microbial isolates from diverse habitats

2011 ◽  
Vol 47 (2) ◽  
pp. 279-287 ◽  
Author(s):  
Angel Treasa Thomas ◽  
Josyula Venkata Rao ◽  
Volety Mallikarjuna Subrahmanyam ◽  
Hariharapura Raghu Chandrashekhar ◽  
Naseer Maliyakkal ◽  
...  
Keyword(s):  
Cell Lines ◽  
Biological Activities ◽  
Bioactive Molecules ◽  
Nuclear Staining ◽  
Cytotoxic Potential ◽  
Biochemical Tests ◽  
Morphological Studies ◽  
Aspergillus Sp ◽  
Mcf 7

Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the cytotoxic potential. Nuclear staining and flow cytometric studies were carried out to assess the potential of the extracts in arresting the cell cycle. The crude ethylacetate extract of isolate F-21 showed promising results by MTT assay with IC50 as low as 20.37±0.36 µg/mL on HeLa, and 44.75±0.81 µg/mL on MCF-7 cells, comparable with Cisplatin. The isolate F-21 was identified as Aspergillus sp. Promising results were also obtained with B-2C and B-4E strains. Morphological studies, biochemical tests and preliminary chemical investigation of the extracts were also carried out.

Sign in / Sign up

Export Citation Format

Share Document