scholarly journals Recent advances in surface plasmon resonance imaging: detection speed, sensitivity, and portability

Nanophotonics ◽  
2017 ◽  
Vol 6 (5) ◽  
pp. 1017-1030 ◽  
Author(s):  
Youjun Zeng ◽  
Rui Hu ◽  
Lei Wang ◽  
Dayong Gu ◽  
Jianan He ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Imaging Techniques ◽  
Detection Sensitivity ◽  
Label Free ◽  
Spr Biosensor ◽  
Recent Advances ◽  
Wavelength Filter ◽  
Detection Speed

AbstractSurface plasmon resonance (SPR) biosensor is a powerful tool for studying the kinetics of biomolecular interactions because they offer unique real-time and label-free measurement capabilities with high detection sensitivity. In the past two decades, SPR technology has been successfully commercialized and its performance has continuously been improved with lots of engineering efforts. In this review, we describe the recent advances in SPR technologies. The developments of SPR technologies focusing on detection speed, sensitivity, and portability are discussed in details. The incorporation of imaging techniques into SPR sensing is emphasized. In addition, our SPR imaging biosensors based on the scanning of wavelength by a solid-state tunable wavelength filter are highlighted. Finally, significant advances of the vast developments in nanotechnology-associated SPR sensing for sensitivity enhancements are also reviewed. It is hoped that this review will provide some insights for researchers who are interested in SPR sensing, and help them develop SPR sensors with better sensitivity and higher throughput.

scholarly journals Kajian Pengaruh Material Graphene pada kinerja Biosensor Berbasis Surface Plasmon Resonance (SPR) pada Deteksi Makanan Halal sebagai Pendukung Halal Research Center UIN Sunan Kalijaga Yogyakarta

2017 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Wida Yanti ◽  
Asih Melati
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Analytical Calculation ◽  
Detection Sensitivity ◽  
Research Center ◽  
Attenuated Total Reflectance ◽  
Muslim Community ◽  
Spr Biosensor

<p><br />Halal foods and medicines are an absolute daily needs for the Muslim community in Indonesia. Therefore the authority institutions in indonesian goverment should ensure the availability of this. It is of course inseparable from the role of higher education through the development of its technology to develop halal detection of foods and drugs. This study is an effort to contribute to the Halal Research Center of UIN Sunan Kalijaga Yogyakarta through the biosensor development in halal detection foods and medicines based on biosensor SPR. This device using graphene materials to improve the detection sensitivity of pork gelatin material that is likely contained in foodstuffs and medicine. From analytical calculation and computation, enhancement of the SPR biosensor performance by involvement graphene it was shown through the ATR (Attenuated Total Reflectance) reflective curve. The result of this results was found the enhancement of the sensitivity 2,86 %.</p><p>Keyword: Surface Plasmon Resonance (SPR), Porcine Gelatin, Graphene, ATR</p>

scholarly journals Recent Advances in Surface Plasmon Resonance Imaging Sensors

Sensors ◽  
2019 ◽  
Vol 19 (6) ◽  
pp. 1266 ◽  
Author(s):  
Dongping Wang ◽  
Jacky Loo ◽  
Jiajie Chen ◽  
Yeung Yam ◽  
Shih-Chi Chen ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Sensor Arrays ◽  
Label Free ◽  
Spr Sensor ◽  
Recent Advances ◽  
Spr Imaging ◽  
Massive Scale ◽  
Imaging Sensors

The surface plasmon resonance (SPR) sensor is an important tool widely used for studying binding kinetics between biomolecular species. The SPR approach offers unique advantages in light of its real-time and label-free sensing capabilities. Until now, nearly all established SPR instrumentation schemes are based on single- or several-channel configurations. With the emergence of drug screening and investigation of biomolecular interactions on a massive scale these days for finding more effective treatments of diseases, there is a growing demand for the development of high-throughput 2-D SPR sensor arrays based on imaging. The so-called SPR imaging (SPRi) approach has been explored intensively in recent years. This review aims to provide an up-to-date and concise summary of recent advances in SPRi. The specific focuses are on practical instrumentation designs and their respective biosensing applications in relation to molecular sensing, healthcare testing, and environmental screening.

scholarly journals On the Use of Surface Plasmon Resonance Biosensing to Understand IgG-FcγR Interactions

2021 ◽  
Vol 22 (12) ◽  
pp. 6616
Author(s):  
Catherine Forest-Nault ◽  
Jimmy Gaudreault ◽  
Olivier Henry ◽  
Yves Durocher ◽  
Gregory De Crescenzo
Keyword(s):  
Surface Plasmon Resonance ◽  
Data Analysis ◽  
Surface Plasmon ◽  
Protein Interactions ◽  
Plasmon Resonance ◽  
Optical Biosensors ◽  
Label Free ◽  
Spr Biosensor ◽  
Therapeutic Monoclonal Antibodies

Surface plasmon resonance (SPR)-based optical biosensors offer real-time and label-free analysis of protein interactions, which has extensively contributed to the discovery and development of therapeutic monoclonal antibodies (mAbs). As the biopharmaceutical market for these biologics and their biosimilars is rapidly growing, the role of SPR biosensors in drug discovery and quality assessment is becoming increasingly prominent. One of the critical quality attributes of mAbs is the N-glycosylation of their Fc region. Other than providing stability to the antibody, the Fc N-glycosylation influences immunoglobulin G (IgG) interactions with the Fcγ receptors (FcγRs), modulating the immune response. Over the past two decades, several studies have relied on SPR-based assays to characterize the influence of N-glycosylation upon the IgG-FcγR interactions. While these studies have unveiled key information, many conclusions are still debated in the literature. These discrepancies can be, in part, attributed to the design of the reported SPR-based assays as well as the methodology applied to SPR data analysis. In fact, the SPR biosensor best practices have evolved over the years, and several biases have been pointed out in the development of experimental SPR protocols. In parallel, newly developed algorithms and data analysis methods now allow taking into consideration complex biomolecular kinetics. In this review, we detail the use of different SPR biosensing approaches for characterizing the IgG-FcγR interactions, highlighting their merit and inherent experimental complexity. Furthermore, we review the latest SPR-derived conclusions on the influence of the N-glycosylation upon the IgG-FcγR interactions and underline the differences and similarities across the literature. Finally, we explore new avenues taking advantage of novel computational analysis of SPR results as well as the latest strategies to control the glycoprofile of mAbs during production, which could lead to a better understanding and modelling of the IgG-FcγRs interactions.

Investigation of an SPR biosensor for determining the influence of connexin 43 expression on the cytotoxicity of cisplatin

The Analyst ◽  
2016 ◽  
Vol 141 (11) ◽  
pp. 3411-3420 ◽  
Author(s):  
Yijia Wang ◽  
Shiwu Zhang ◽  
Chunze Zhang ◽  
Zhenying Zhao ◽  
Xiaoli Zheng ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Connexin 43 ◽  
Label Free ◽  
The Real ◽  
Spr Biosensor ◽  
Genes Expression ◽  
Label Free Detection

The real-time and label free detection abilities of surface plasmon resonance (SPR) biosensors provide a way of evaluating the influence of some genes’ expression on anti-tumor drug cytotoxicity.

Plasma enhanced label-free immunoassay for alpha-fetoprotein based on a U-bend fiber-optic LSPR biosensor

RSC Advances ◽  
2015 ◽  
Vol 5 (31) ◽  
pp. 23990-23998 ◽  
Author(s):  
Gaoling Liang ◽  
Zhongjun Zhao ◽  
Yin Wei ◽  
Kunping Liu ◽  
Wenqian Hou ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Fiber Optic ◽  
Cost Effective ◽  
Alpha Fetoprotein ◽  
Localized Surface Plasmon ◽  
Label Free

A simple, label-free and cost-effective localized surface plasmon resonance (LSPR) immunosensing method was developed for detection of alpha-fetoprotein (AFP).

scholarly journals Modifications of the PAMONO-Sensor Help to Size and Quantify Low Number of Individual Biological and Non-Biological Nano-Particles

2021 ◽  
Vol 6 (1) ◽  
pp. 26
Author(s):  
Rahat Morad Talukder ◽  
Al Shahriar Hossain Rakib ◽  
Julija Skolnik ◽  
Zohair Usfoor ◽  
Katharina Kaufmann ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Nano Particles ◽  
Label Free ◽  
Specific Detection ◽  
Virus Like Particles ◽  
Low Concentrations ◽  
Challenging Tasks ◽  
Image Area

In a series of recently published works, we demonstrated that the plasmon-assisted microscopy of nano-objects (PAMONO) technique can be successfully employed for the sizing and quantification of single viruses, virus-like particles, microvesicles and charged non-biological particles. This approach enables label-free, but specific detection of biological nano-vesicles. Hence, the sensor, which was built up utilizing plasmon-assisted microscopy, possesses relative versatility and it can be used as a platform for cell-based assays. However, one of the challenging tasks for such a sensor was the ability to reach a homogeneous illumination of the whole surface of the gold sensor slide. Moreover, in order to enable the detection of even relatively low concentrations of nano-particles, the focused image area had to be expanded. Both tasks were solved via modifications of previously described PAMONO-sensor set ups. Taken together, our latest findings can help to develop a research and diagnostic platform based on the principles of the surface plasmon resonance (SPR)-assisted microscopy of nano-objects.

scholarly journals Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 102 ◽  
Author(s):  
Richard Schasfoort ◽  
Fikri Abali ◽  
Ivan Stojanovic ◽  
Gestur Vidarsson ◽  
Leon Terstappen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mammalian Cells ◽  
Living Cells ◽  
Label Free ◽  
Intact Cells ◽  
Cell Parameters ◽  
Spr Imaging ◽  
Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

scholarly journals Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 130 ◽  
Author(s):  
Georgina Ross ◽  
Maria Bremer ◽  
Jan Wichers ◽  
Aart van Amerongen ◽  
Michel Nielen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Speed ◽  
Selection Process ◽  
Low Cost ◽  
Real Life ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Label Free

Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.

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