scholarly journals Effects of Tamarindus indica (Linnaeus 1753) pulp-fortified diets on the gut microflora and morphometry in African catfish Clarias gariepinus (Burchell 1822)

2021 ◽  
Vol 6 (2) ◽  
pp. 45-51
Author(s):  
Olarinke Victoria Adeniyi ◽  
Flora Eyibio Olaifa ◽  
Benjamin Obukowho Emikpe ◽  
Samuel Temitope Ogunbanwo

The global criticism on the adverse effects of synthetic antibiotics including destabilization of gut microflora necessitated research into natural alternative like phytobiotics. Tamarind pulp is a phytobiotic known for its antimicrobial, growth-promoting and antioxidants properties, with little information on the mechanism of its growth promotion in fish. Therefore, this study investigated the effects of tamarind pulp (TP) meal as feed additives on the growth, gut microflora, and morphometry in Clarias gariepinus. The fish were fed with diets fortified with graded levels (0.0%, 0.5%, 1.0%, 1.5% or 2.0%) of TP or 0.2% oxytetracycline (OTC). Fish weight gain, feed conversion ratio (FCR), gut microflora and morphometry were determined. The weight gain of fish rose (P 0.05) with increasing levels of TP, while the FCR reduced significantly, compared to those fed the control diets. The total viable counts and enterobacteriaceae from the gut of fish fed OTC-fortified control diet was lower, compared the fish fed unfortified control (0.0% TP) diet and diets containing TP. Fish fed 1.0-2.0% TP-fortified diets had significantly higher yeast counts, compared to those fed other diets, while on the other hand higher (P0.05) Streptococcus sp was obtained from fish fed the control diets. The thickness of muscular lining of the base of villi, villi height and absorption area were higher (P ˂ 0.05) in fish fed OTC-fortified (C2) diet, compared to other treatments, while lower crypt depth were obtained in fish fed TP-fortified diets. The correlation analyses showed moderate negative (R = -0.539 at P 0.05) relationship between FCR and population of enterobacteriaceae while a very strong positive relationship (R = 0.848 at P 0.01) existed between FCR and population of haemolytic Streptococcus. The enhanced growth in fish fed TP-fortified diets could be accredited to the reduced crypt depth and colonization of yeast and reduced Streptococcus counts. Therefore the application of tamarind pulp meal at 2% is recommended for enhanced growth and healthy gut microflora composition.

2020 ◽  
Vol 78 (1) ◽  
pp. 11-20
Author(s):  
Sunday Emmanuel Olusola ◽  
Igbekele Joshua Ajiwoju ◽  
Benjamin Obukowho Emikpe

AbstractThis study investigated the efficacy of Tamarind Leaves (TL) and Mango Leaves (ML) with Oxytetracycline (OXY) on growth performance, haematoimmunological and disease resistance of Clarias gariepinus juveniles against Aeromonas hydrophila. Experimental diets consist of control (0%), TL2 (1%), TL3 (2%), ML4 (1%), ML5 (2%), OXY6 (30mg/kg diet), (TL+ML) 7, (TL+OXY) 8, (ML+OXY) 9 and (TL+ML+OXY) 10. The fish (3.02±0.01g) were replicated twice with 20 fish per replicate and were fed twice daily at 3% body weight of 40% crude protein for twelve weeks (8 weeks for feeding trial and 4 weeks for challenge test). Mean Weight Gain (MWG), Specific Growth Rate (SGR), Packed Cell Volume (PCV), Haemoglobin (Hb), Lymphocytes (LYM), Globulin (GLO), Amino Alanine Transferase (ALT) and Aspertate Amino Transferase (AST) contents were ascertained using standard technique. The fish were infected with A. hydrophila at 5.94 log10 CFU/ml interperitoneally and fed different diets to evaluate their Relative Percent of Survival (RPS). Data was subjected to descriptive statistics and one-way analysis of variance at P=0.05. Clarias gariepinus juveniles fed treated diets had higher growth rates than the control diet but C. gariepinus fed (TL+ML+OXY) 10 had a significantly higher MWG and SGR of 7.74±0.69 g and 0.97±0.01 g, respectively. The PCV (44.0±2.00%), Hb (14.7±2.00 g/dl), LYM (37.0±2.00), GLO (42.0 ±2.00 g/dl) were higher in the C. gariepinus fed (TL+ML+OXY) 10 than the control diet. The AST and ALT values among the treated groups were lower than the values in the control at the post-challenge test. The RPS against A. hydrophila was higher in the treated groups (100%) than in the control (0%). Fish fed tamarind and mango leaves had enhanced mean weight gain and were more resistant to A. hydrophila infection.


2020 ◽  
pp. 73-79
Author(s):  
Ayodeji Benjamin Agbanimu ◽  
Eunice Olwayemisi Adeparusi

A feeding trial was conducted to examine the dietary effects of using African Palm weevil in the diet of Catfish. Five experimental diets containing 40% crude protein were formulated, four of these diets contained defatted African palm weevil larvae meal at varying inclusion levels denoted as APW25%, APW50%, APW75% and APW100% while the diet with fish meal (APW0%) served as control diet. The experiment was conducted in triplicates for 10 weeks using 15 aquaria glass tanks with 10 fish per tank. The percentage weight gain was significantly different (P>0.05) across the fish fed experimental diets, with the highest percentage weight gain (1118.30g) recorded in fish fed diet APW100 and the lowest percentage weight gain (749.40g) recorded in fish fed diet FM. The feed conversion ratio (FCR) were not significantly different (P<0.05) across the experimental diet. The highest FCR value (1.48±0.42) was recorded in the fish fed diet FM while the lowest FCR value (1.06±0.01) was recorded in fish fed diet APW100. There were no significant differences (P>0.05) in the PER among the experimental diets. It could be concluded that defatted African palm weevil larvae meal in the diet in replacement of fishmeal.in the diet Clarias gariepinus juvenile.


2019 ◽  
Vol 1 ◽  
pp. 113-117 ◽  
Author(s):  
O A Aghoghovwia ◽  
S T Obah ◽  
E I Ohimain

Duckweed (Lemna gibba), a nuisance aquatic macrophyte, was studied as a substitute for the partially replacement ofsoya bean meal in the diet of Clarias gariepinus fingerlings. Four dry diets formulated at 4% crude protein were fed to fingerlings at 0% (control) 10%, 20% and 30% inclusion levels of duckweed meal named as Diet A, B, C and D respectively. The fish were feed at 5% body weight and was assessed for feed conversion ratio (FCR), protein efficiency ratio (PER), survival rate among others. The highest growth performance and nutrient utilization was recorded at diet D i.e. 30% inclusion rate of duckweed with a mean weight gain of 5.45 + 0.309g, and food conversion ratioof 3.32 + 0.43 which was significantly different (P < 0.05) to the values obtained for other diets. The lowest weight gain was recorded in diet A i.e. the control diet, with a value of 3.06 + 0.03g. The result of this study showed that as the inclusion rate of duckweed is increased, there was a corresponding increase in the growth rate of the fish. The study demonstrated that duckweed can be used for the replacement of soya beans in the diet of catfish fingerlings, thus converting a nuisance weed into useful feed component for the reduction of feeding cost.


2020 ◽  
Vol 142 ◽  
pp. 147-160
Author(s):  
HH Mahboub ◽  
K Shahin ◽  
AW Zaglool ◽  
EM Roushdy ◽  
SAA Ahmed

Zinc (Zn) is an important trace element in fish diets that is required for growth, immunity and antioxidant defense mechanisms. The current study assessed the effects of both organic and nanoparticle zinc oxide (ZnO and ZnO-NPs, respectively) on growth performance, immune response and the antimicrobial effect against Pseudomonas aeruginosa in African catfish Clarias gariepinus. Fish were fed either a control diet or diets supplemented with organic ZnO at concentrations of 20 and 30 mg kg-1 or ZnO-NPs at concentrations of 20 and 30 mg kg-1. After 60 d, a subset of the fish was injected intraperitoneally with 3 × 107 CFU ml-1 of P. aeruginosa. Results showed that body weight gain, feed conversion ratio and specific growth rates were significantly increased in ZnO-NPs20 compared to all other groups. The dietary supplementation with 20 mg kg-1 of ZnO-NPs improved the antioxidant status of fish. Moreover, IgM, lysozyme and nitric oxide showed a significant increase in the fish which received the ZnO-NPs20-supplemented diet. A significant upregulation of growth and stress-related genes was seen in the ZnO-NPs20-supplemented group compared to other groups. However, there was no significant difference in the expression of immune-related genes among ZnO-NPs20, ZnO-NPs30 and ZnO30 groups. These findings highlight the potential use of nano-ZnO for improving growth performance, antioxidant status, immunological status and antibacterial activity against P. aeruginosa in African catfish.


2021 ◽  
Vol 43 (1) ◽  
pp. 385-398
Author(s):  
S. I. Ajibola ◽  
S. O. Obasa ◽  
A. K. Akintokun ◽  
I. Abdulraheem

Immuno-nutrition studies have shown that some nutrients are linked to the immunological status of fish. Growth performance, nutrient utilization and intestinal microflora were examined in Clarias gariepinus. The 120 C. gariepimus fingerlings (weight, 2.33±0.07g)were fed Aloe barbadensis leaves-paste supplemented diets in 40L freshwater-filled plastic tanks ad libitum twice daily for 12 weeks. The experimental diets containing 40% crude protein were supplemented with three concentration of A. barbadensis leaves-paste: ABL1-1%; ABL2-2%; ABL3-3% and control-0%. Mean weight gain (MWG) and percentage weight gain (PWG) increased (p<0.05) as the concentration of A. barbadensis increased. MWG (17.95±0.78) and PWG (772.2±54.94) were highest in fish fed ABL3 and lowest MWG (11.92±1.16) and PWG (17.95±0.78) in fish fed control diet. Nutrients were better utilized among the diets supplemented group at different significant levels (p < 0.05). The highest value of ANPU was observed in ABL1 when compared to all other treatments including the control. Survival rate decreased as concentration of paste increased. There were no significant differences (p>0.05) in the total bacterial counts (TBC) in A. barbadensis leaves-paste supplemented diets and the control having the highest TBC (23.67 ±0.88 x 105 CFU/ml). Growth of total fungal counts (TFC) was not observed in ABLI. There was a reduction in TFC as the concentration increased in the other supplemented diets, and the control having the highest TFC (7.67 ± 0.44 x 105CFU/ml). The study concluded that inclusion of 1% A. barbadensis leaves-paste as supplement in the diet could effectively improve the growth performance, nutrient utilization and survival of cultured C. gariepinus. A. barbadensis leaves-paste could also reduce the microbial load of the fish.


2021 ◽  
Vol 14 (3) ◽  
pp. 027-035
Author(s):  
Sunday Kenneth Igbang ◽  
Ada Fidelis Bekeh ◽  
Ochang Stephen Ncha

Aiming at finding cheaper and still efficient feed for aquaculture species 56 days feeding trial was conducted on African catfish (Clarias gariepinus) post fingerlings, fed on five diets with a 40% crude protein target in which maize was replaced with maize offal meal at 0%, 25%, 50%, 75%, and 100% respectively. Ten fish each were stocked in a plastic aquarium tank containing 20 L of water. The fish were fed at 5% body weight divided into two rations ginen at the hours of 8-8:30 am and 5-5:30pm. Growth parameters showed increase with increase in maize offal based diets, with observed weight gains of 72.42, 79.00, 95.92, 130.83 and 65.83 for diets 1, 2, 3, 4, and 5 respectively. Feed conversion ratio (recorded best in 75% maize offal based diet substitution, while the poorest was recorded at 25% maize offal diet replacement, followed by the control diet (100% maize diet). 75% maize offal replacement recorded the highest specific growth rate (p<0.05).There was significant difference (p<0.05) in the ash content, crude fiber and energy level in the fish carcass. The cost of feed used throughout the experimental period was not significantly (p>0.05) influenced by the increasing level of maize offal as a replacement for maize in the diets, as the costs were comparable. However, this study reveals that maize offal meal can successfully replaced maize up to 75% in the diet of Clarias gariepinus post fingerlings without adverse effect on growth, carcass composition and economics.


2021 ◽  
Vol 43 (2) ◽  
pp. 385-398
Author(s):  
S. I Ajibola ◽  
S. O Obasa ◽  
A. K Akintokun ◽  
I Abdulraheem

Immuno-nutrition studies have shown that some nutrients are linked to the immunological status of fish. Growth performance, nutrient utilization and intestinal microflora were examined in Clarias gariepinus. The 120 C. gariepinus fingerlings (weight, 2.33±0.07g) were fed with Aloe barbadensis leaves-paste supplemented diets in 40L freshwater-filled plastic tanks ad libitum twice daily for 12 weeks. The experimental diets containing 40% crude protein were supplemented with three concentration of A. barbadensis leaves-paste: ABL1–1%; ABL2–2%; ABL3–3% and control–0%. Mean weight gain (MWG) and percentage weight gain (PWG) increased (p<0.05) as the concentration of A. barbadensis increased. MWG (17.95±0.78) and PWG (772.2±54.94) were highest in fish fed ABL3 and lowest MWG (11.92±1.16) and PWG (17.95±0.78) in fish fed control diet. Nutrients were better utilized among the diets supplemented group at different significant levels (p < 0.05). The highest value of ANPU was observed in ABL1 when compared to all other treatments including the control. Survival rate decreased as concentration of paste increased. There were no significant differences (p>0.05) in the total bacterial counts (TBC) in A. barbadensis leaves-paste supplemented diets and the control having the highest TBC (23.67 ± 0.88 x 105CFU/ml). Growth of total fungal counts (TFC) was not observed in ABL1. There was a reduction in TFC as the concentration increased in the other supplemented diets, and the control having the highest TFC (7.67 ± 0.44 x 105 CFU/ml). The study concluded that inclusion of 1% A. barbadensis leaves-paste as supplement in the diet could effectively improve the growth performance, nutrient utilization and survival of cultured C. gariepinus. A. barbadensis leaves-paste could also reduce the microbial load of the fish.


2020 ◽  
Vol 45 (5) ◽  
pp. 112-121
Author(s):  
C. P. Njoku ◽  
A. O. Fasakin ◽  
T. R. Ayano ◽  
F. T. Aweda ◽  
O. O. Amaihian ◽  
...  

This study was carried out to investigate growth performance and cost benefits of growing pigs on different herbal-mix feed additives and weight asymmetry. Fifty-four, 8 weeks old Large White breed of pigs with an average body weight of 15.33±0.2 kg were randomly assigned to nine treatments with each treatment consisting of three replicates of two pigs each. Three diets containing different herbal-mix (Control diet without herbal-mix, moringa-basil meal diet, neem-basil meal diet) were formulated and fed to the pigs for sixteen weeks duration of the study. The moringa-basil and neem-basil leaf meals mixtures were in the ratio of 10 to 2; that is ten percent (10%) moringa or neem to two percent (2%) basil. Parameters determined include growth indices and cost benefits. Data collected were subjected to two-way analysis of variance using SAS (2000). Pigs on control diet had the highest final weight (49. 44, 43.22, 40.67 kg) and daily weight gain (515.87, 401.59, 388.89 g/pig) and the best feed conversion ratio (3.56, 4.59, 4.23) when compared to the values documented for pigs on ration containing different herbal-mix feed additives. The least total feed cost and revenue per pig were noted for pigs on neem-basil dietary treatment while the highest values were documented for pigs on control ration. Homogenous light weight pigs had higher feed intake and total feed cost per pigs when compared to other values noted for pigs on other treatment groups. Heterogeneous weight pigs recorded better feed conversion ratio, lower feed cost per kilogram weight gain and higher gross margin values when compared to the values noted for their homogenous counterparts. It can be concluded from this study that performance indices of growing pigs were affected by the dietary inclusion of herbal-mix feed additives and weight asymmetry. Hence, herbal-mix leaf meals can be integrated into growers ration in order to lower production cost, likewise,unevenly weight growing pigs can be managed together in order to obtain higher production efficiency in terms of feed intake and cost of production.


2020 ◽  
Vol 47 (1) ◽  
pp. 129-139
Author(s):  
S. I. Adegbesan ◽  
I. Abdulraheem

Dietary supplementation with phytobiotics is a promising area in fish nutrition towards promoting the growth and health status of cultured fish. This experiment was conducted to determine the effects of Aspilia africana leaves-paste (AAL) on the growth, haematology and serum biochemistry of Clarias gariepinus broodstock. Experiment was carried out in a 24 net-happa (0.6m x 1.07m x 1.2m) suspended in an earthen pond (30 m x 5 m x 1.2m). Forty-eight (7 months old fish, 24 males (0.80±0.04kg) and 24 females (0.70±0.03kg)) were stocked at two fish per net-happa under four treatments in six replicates in a completely randomized design. Four diets (40% crude protein) were formulated to contained: control (0%); AAL1 (0.5%); AAL2 (1%) and AAL3 (1.5%). Fish were fed ad libitum twice daily for 16 weeks. Data on all parameters were analyzed using ANOVA. The highest mean weight gain, MWG: 3.13±0.15kg and lowest feed conversion ratio, FCR: 1.30± 0.04 were recorded in broodstock fed 1.5% AAL3. The lowest MWG (1.27±0.03kg) and highest FCR: 1.72±0.03) were obtained in broodstock fed control diet. The lowest packed cell volume: 15.83 ± 0.17 % and haemoglobin: 5.25 ± 0.1 (g/dL) were recorded in fish fed 1% and 1.5% AAL. No significant differences in the total protein and creatinine values obtained between fish fed 1% and 1.5% AAL. The study recommended the dietary inclusion of 1.5% A. africana leaves-paste to effectively promote growth and nutrient utilization of cultured C. gariepinus broodstock


2017 ◽  
Vol 7 (3) ◽  
pp. 180-185
Author(s):  
M. Yu. Sychov ◽  
M. I. Holubiev ◽  
V. V. Kovalchuk ◽  
Yu. V. Pozniakovskyi ◽  
T. A. Holubieva ◽  
...  

<p>Experimental studies conducted in terms of problem research laboratory of feed additives National University of Life and Environmental Sciences of Ukraine. Independent experiment was conducted with growing Pharaoh Coturnix quails to determine the optimal valine-to-protein ratio for quail development. We conducted a randomized block experiment with 4 treatments, each with 5 replicates of 25 growing birds (1 to 35 d of age). A diet consisting of soybean and sunflower meal, wheat, corn, Fish meal, sunflower oil, concentrate (28% CP, 2.88 kcal/g on 1 to 21d of age, 20.5 % CP, 2.97 kcal/g on 22 to 35 d of age) having severally 1.54 and 1.33 % valine served as basal feed. Valine was supplemented in four graded levels up to 2.10 g/100 g on 1 to 21d of age and 1.54 g/100 g on 22 to 35 d of age at the expense of L-valine. Diets and water were offered ad libitum.</p><p>Body weight gain and feed/gain ratio were studied. Quails significantly responded to the increasing valine concentration in growth, feed/gain ratio. Analysis was used to calculate the Valine nutrient recommendations. These estimates were 1.28 % for the starter and 1.23 % for the total Val for the grower period. Supplementation with Valine to the base diet resulted in body weight gain of quails equal to those fed the control diet (p&lt;0.01). Feed conversion values of quails supplemented with Valine, resulted in an improvement on 2.3 %.</p>


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