Omental Leiomyoma in a Male Adult Horse

2007 ◽  
Vol 44 (5) ◽  
pp. 722-726 ◽  
Author(s):  
D. Schaudien ◽  
J. M. V. Müller ◽  
W. Baumgärtner
Keyword(s):  
Smooth Muscle ◽  
Gastrointestinal Tract ◽  
Tumor Cells ◽  
Smooth Muscle Actin ◽  
Neuron Specific Enolase ◽  
Muscle Actin ◽  
Male Adult ◽  
S 100 ◽  
Multiple Cysts ◽  
Histology And Immunohistochemistry

A well-circumscribed mass 70 X 35 X 28 cm in size and 41 kg in weight was detected at necropsy in a male adult horse within the omentum major without any association to the gastrointestinal tract. The tumor consisted of multiple white-to-yellow lobules and displayed a firm consistency. In addition, multiple cysts filled with blood-like fluid, and multifocal areas of necrosis were observed. Histologically, the mass consisted of slightly pleomorphic spindloid-shaped cells arranged in interlacing bundles containing elongated nuclei with blunt ends. The majority of tumor cells revealed a positive immunoreaction for α-smooth muscle actin, vimentin, and neuron-specific enolase and were negative for S-100, factor VIII-related antigen, and glial fibrillary acidic protein. Few tumor cells showed expression of desmin and c-kit. On the basis of macroscopy, histology, and immunohistochemistry, an omental leiomyoma was diagnosed.

scholarly journals Piloleiomyosarcoma in cats: Histological and immunohistochemical features

2021 ◽  
pp. 030098582110425
Author(s):  
Francisco Rodríguez Guisado ◽  
Pedro Luis Castro
Keyword(s):  
Smooth Muscle ◽  
Subcutaneous Tissue ◽  
Smooth Muscle Actin ◽  
Neuron Specific Enolase ◽  
Surgical Excision ◽  
Giant Cells ◽  
Hair Follicles ◽  
Muscle Actin ◽  
Neoplastic Cells ◽  
S 100

This study describes the histomorphology and immunohistochemical profile of 9 cases of feline piloleiomyosarcoma. Cats ranged in age from 7 to 16 years (mean 10), and tumors were 7 to 24 mm in diameter (mean 15). Tumors were composed of fusiform cells that were haphazardly arranged or in variably sized interwoven bundles. Neoplastic cells had eosinophilic and fibrillar cytoplasm, and elongated blunt-ended nuclei. Entrapment of hair follicles and absence of vascular components support an origin from the smooth muscle cells of the arrector pili. Additional findings included bizarre nuclei and giant cells (7/9 cases), atypical mitoses (7/9 cases), ulceration (3/9 cases), and intratumoral necrosis (6/9 cases). Neoplastic cells expressed calponin, desmin, α-smooth muscle actin, and vimentin, but not CD18, CD31, cytokeratins, glial fibrillary acidic protein, neuron-specific enolase, Melan A, p63, or S-100 protein. Surgical excision was curative in 6/9 cases, with local recurrence in 2/9 cases and metastasis to local lymph nodes in 1/9 case. Clinical outcome was influenced by mitotic count, infiltration of subcutaneous tissue, and intensity of nuclear immunolabeling for p53.

scholarly journals Ca2+-dependent regulation of vascular smooth-muscle caldesmon by S.100 and related smooth-muscle proteins

1991 ◽  
Vol 277 (3) ◽  
pp. 819-824 ◽  
Author(s):  
K Pritchard ◽  
S B Marston
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Bovine Brain ◽  
Protein Yield ◽  
Muscle Actin ◽  
Muscle Proteins ◽  
Related Protein ◽  
Thin Filaments ◽  
S 100 ◽  
Related Proteins

1. We have investigated the ability of bovine brain S.100, and of three related proteins from sheep aorta smooth muscle, to confer Ca(2+)-sensitivity on thin filaments reconstituted from smooth-muscle actin, tropomyosin and caldesmon. 2. At 37 degrees C in pH 7.0 buffer containing 120 mM-KCl, approximately stoichiometric amounts of S.100 reversed caldesmon's inhibition of the activation of myosin MgATPase by smooth-muscle actin-tropomyosin. The [S.100] which reversed by 50% the inhibition by caldesmon (the E.C.50) was 2.5 microM when [caldesmon] = 2-3 microM in the assay mixture. When [KCl] was decreased to 70 mM, E.C.50 = 11.5 microM; at 25 degrees C in 70 mM-KCl, up to 20 microM-S.100 had no effect. When skeletal-muscle actin rather than smooth-muscle actin was used to reconstitute thin filaments, 20 microM-S.100 did reverse inhibition by caldesmon, at 25 degrees C in buffer containing 70 mM-KCl. This dependence on conditions is also characteristic of the calmodulin-caldesmon interaction. 3. These results suggested that S.100 or a related protein might interact with caldesmon in smooth muscle. We therefore attempted to prepare such a protein from sheep aorta. Three proteins were purified: an Mr-17,000 protein (yield 16 mg/kg), an abundant Mr-11,000 protein (yield 48 mg/kg), and an Mr-9000 protein (yield 4 mg/kg). Neither of the last two low-Mr proteins had any effect on activation of myosin MgATPase by reconstituted thin filaments. The protein of Mr 17,000 had Ca(2+)-sensitizing activity, and behaved exactly like brain calmodulin in the assay system.

Gastrointestinal Stromal Tumors and Leiomyomas in the Dog: A Histopathologic, Immunohistochemical, and Molecular Genetic Study of 50 Cases

2003 ◽  
Vol 40 (1) ◽  
pp. 42-54 ◽  
Author(s):  
D. Frost ◽  
J. Lasota ◽  
M. Miettinen
Keyword(s):  
Smooth Muscle ◽  
Gastrointestinal Stromal Tumors ◽  
Smooth Muscle Actin ◽  
Abdominal Cavity ◽  
Clinical Signs ◽  
Muscle Actin ◽  
Molecular Genetic Study ◽  
Stromal Tumors ◽  
Pathologic Features ◽  
S 100

Fifty canine gastrointestinal (GI) mesenchymal tumors were examined to determine the occurrence of leiomyomas (LM) and GI stromal tumors and to compare their clinicopathologic features. Twenty-one tumors (42%) were histologically reclassified as gastrointestinal stromal tumors (GISTs) and 29 tumors (58%) as LMs on the basis of their histologic similarity with homologous human tumors. The GISTs occurred equally in males and females, with a mean age of 11 years (range 5–14 years). Five GISTs (24%) were associated with clinical signs and six (29%) had metastasis in liver or abdominal cavity. The GISTs occurred in large intestine (10, 48%), small bowel (six, 29%), stomach (four, 19%), and mesentery of small intestine (one, 5%). Histologically, they were highly cellular spindle, or less commonly epithelioid tumors with mitotic rates ranging from 0 to 19 per 10 HPF. Eleven tumors (52%) were positive for CD117 (KIT); seven (33%) were positive for smooth muscle actin but none for desmin and S-100 protein. Sequences of KIT exon 11, often mutated in human GISTs, were evaluated from four GISTs. Deletion of Try556-Lys557 coexisting with duplication of Gln555 in one case of GIST and T to C transition resulting in substitution of Pro for Leu575 in another were identified. The LMs occurred predominantly in males (82%) with a mean age of 11 years (range 8–17 years). Nine tumors (31%) had associated clinical signs. They occurred in the stomach (22, 76%), esophagus (four, 14%), and intestines (three, 10%); all were paucicellular, had no mitoses, and were composed of mature smooth muscle cells. Twenty-eight (97%) were positive for smooth muscle actin and 18(62%) for desmin but none for CD117 and S-100. Both GISTs and true LMs occur in the GI tract of dogs. Both tumors have distinctive pathologic features.

Glomus Tumor in the Digit of a Cat

2002 ◽  
Vol 39 (5) ◽  
pp. 590-592 ◽  
Author(s):  
K. Uchida ◽  
R. Yamaguchi ◽  
S. Tateyama
Keyword(s):  
Glomus Tumor ◽  
Smooth Muscle Actin ◽  
Neuron Specific Enolase ◽  
Outer Side ◽  
Muscle Actin ◽  
Epithelioid Cells ◽  
Alpha Smooth Muscle Actin ◽  
The Third ◽  
Pathologic Features ◽  
S 100

A solitary mass approximately 1.5 X 2 cm located on the outer side of the third digit of the left forepaw of a 7-year-old male cross-breed cat was examined pathologically. The excised tumor mass was hard and white and located within the deep dermis and subcutis. Histopathologically, the mass consisted of a mixed population of small round epithelioid cells arranged in ribbon- or cordlike structures and spindle-shaped cells forming loose irregular bundles in a mucinous stroma. The epithelioid cells were often arranged around small blood vessels. Neoplastic cells were intensely positive for vimentin and alpha smooth muscle actin and negative for keratin, desmin, S-100 protein, and neuron-specific enolase. Based on these pathologic features, the tumor was diagnosed as a glomus tumor, a neoplasm not previously reported in cats and extremely rare in animals.

Immunohistochemical Staining Characteristics of Canine Gastrointestinal Stromal Tumors

1997 ◽  
Vol 34 (4) ◽  
pp. 303-311 ◽  
Author(s):  
R. G. LaRock ◽  
P. E. Ginn
Keyword(s):  
Smooth Muscle ◽  
Small Intestine ◽  
Immunohistochemical Staining ◽  
Gastrointestinal Stromal Tumors ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Stromal Tumors ◽  
S 100 ◽  
Microscopic Features ◽  
Microscopic Diagnosis

Sections from 35 formalin-fixed, paraffin-embedded, canine gastrointestinal stromal tumors consisting of 14 leiomyomas (five stomach, three small intestine, two colon, four rectum), 18 leiomyosarcomas (one stomach, five small intestine, nine cecum, three rectum), two undifferentiated sarcomas (two stomach), and one neurofibrosarcoma (small intestine) were examined for the expression of vimentin, S-100 protein, α-smooth muscle actin, and desmin via immunoperoxidase methodology using an avidin-biotin complex technique. The leiomyomas were 4/14 (29%) vimentin-positive, 3/14 (21%) S-100 protein-positive, 10/14 (71%) α-smooth muscle actin-positive and 13/14 (93%) desmin-positive. Leiomyosarcomas were 18/18 (100%) vimentin-positive, 11/18 (61%) S-100 protein-positive, 9/18 (50%) α-smooth muscle actin-positive, and 15/18 (83%) desmin-positive. The undifferentiated sarcomas were 2/2 (100%) vimentin-positive, 2/2 (100%) S-100 protein-positive, 1/2 (50%) α-smooth muscle actin-positive, and 0/2 (0%) desmin-positive. The neurofibrosarcoma was vimentin and S-100 protein-positive and α-smooth muscle actin- and desmin-negative. Thirty-one of thirty-five (89%) of all neoplasms demonstrated reactivity for either desmin and/or α-smooth muscle actin. S-100 protein reactivity occurred in 17/35 (49%) of all specimens. Lack of desmin and α-smooth muscle actin reactivity occurred in 4/35 (11%) of all specimens, all of which were vimentin-positive. The immunohistochemical results indicate that the majority of canine gastrointestinal stromal tumors (GIST) with light microscopic features of smooth muscle cells have immunohistochemical staining patterns supporting smooth muscle differentiation. Vimentin reactivity correlated with a light microscopic diagnosis of malignancy. The lack of smooth muscle cell markers in some tumors and the high percentage of cases positive for S-100 protein may suggest a more complex histogenesis or differentiation for subgroups of these tumors.

Pulmonary Myxoma in a Sheep

2009 ◽  
Vol 46 (3) ◽  
pp. 457-459 ◽  
Author(s):  
F. Ilhan ◽  
Z. Yener
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Left Lung ◽  
Stellate Cells ◽  
Muscle Actin ◽  
Neoplastic Cells ◽  
S 100 ◽  
Myxoid Matrix ◽  
Uncommon Neoplasm

Pulmonary myxoma is an uncommon neoplasm. A pale tan, lobulated, and well-circumscribed mass was discovered at slaughter in the left lung of a 5-year-old sheep. Histologically, the tumor was composed of spindloid to stellate cells in a myxoid matrix. Neoplastic cells were immunohistochemically positive for vimentin but did not express cytokeratins, S-100 protein, smooth muscle actin, desmin, or p53. On the basis of the histologic and immunohistochemical findings, this tumor was diagnosed as a myxoma.

Orbital (Retrobulbar) Meningioma in a Simmental Cow

2007 ◽  
Vol 44 (4) ◽  
pp. 504-507 ◽  
Author(s):  
J. L. Reis ◽  
C. T. Kanamura ◽  
G. M. Machado ◽  
R. O. França ◽  
J. R. J. Borges ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Epithelial Membrane Antigen ◽  
Smooth Muscle Actin ◽  
Neuron Specific Enolase ◽  
Muscle Actin ◽  
Transmission Electron ◽  
S 100 ◽  
Poorly Differentiated ◽  
The Right ◽  
Mib 1

A 12-year-old Simmental cow was presented with a moderately firm irregular whitish mass of approximately 5 cm in diameter, occupying the right orbit. Microscopically, a poorly differentiated neoplasm was observed. The immunohistochemical panel included cytokeratins, vimentin, epithelial membrane antigen, Factor VIII, CD34, Mart-1, Melan A, smooth muscle actin, desmin, chromogranin, neuron-specific enolase, S-100 protein, and MIB-1. The neoplasm was negative for all of them, with the exception of vimentin and S-100 protein. Transmission electron microscopy revealed abundant desmosomes. These findings support the diagnosis of orbital (retrobulbar) meningioma.

Basaloid Squamous Cell Carcinoma of the Esophagus With or Without Adenoid Cystic Features

2004 ◽  
Vol 128 (10) ◽  
pp. 1124-1130 ◽  
Author(s):  
Tie-Jun Li ◽  
Yong-Xin Zhang ◽  
Julie Wen ◽  
Daniel F. Cowan ◽  
John Hart ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Smooth Muscle ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Squamous Cell ◽  
Smooth Muscle Actin ◽  
Basaloid Squamous Cell Carcinoma ◽  
Muscle Actin ◽  
Adenoid Cystic ◽  
Basaloid Squamous

Abstract Context.—Basaloid squamous cell carcinoma (BSCC) of the esophagus is a rare malignant tumor that morphologically could bear some resemblance to adenoid cystic carcinoma (ACC) originating from salivary glands. Objective.—The purpose of this study is to describe the histologic, immunohistochemical, and ultrastructural findings of BSCCs of the esophagus, with an emphasis on comparing tumors with or without adenoid cystic features. Design.—We reviewed 239 cases of primary esophageal carcinoma and detected 12 cases (5%) of BSCC. The light and electron microscopic findings and immunocytochemical localization of various antigens, including cytokeratins (AE1, AE3), carcinoembryonic antigen, epithelial membrane antigen, S100, smooth muscle actin, and p53, were examined in these BSCC cases. Results.—Histologically, all BSCCs were composed of solid lobules or nests of basaloid cells with well-demarcated outlines surrounded by a fibrous stroma. Seven of 12 tumors showed areas of ACC-like features, that is, cribriform-like pseudoglandular lumina formation and hyaline material surrounding the tumor nests, whereas the remaining 5 tumors were apparently pure basaloid carcinomas. These 2 groups of tumors were histologically and immunohistochemically identical in many aspects, namely, high-grade nuclei of the tumor cells with frequent mitoses, abundant comedo-type necrosis, focal areas of concomitant squamous differentiation, consistent immunoreactivity for cytokeratins, and poor or absent staining for S100 and smooth muscle actin. Ultrastructurally, the basaloid tumor cells exhibited relatively undifferentiated cellular characteristics and undeveloped cell organelles. Conclusion.—Basaloid squamous cell carcinomas of the esophagus frequently have an intimate association with ACC-like patterns, but their histologic, immunocytochemical, and ultrastructural features correspond more to poorly differentiated squamous cell carcinoma than to salivary gland ACC. This distinction is important because genuine ACC is much less aggressive than BSCC.

scholarly journals Anterior Mediastinal Leiomyosarcoma: A Case Report and Literature Review

2021 ◽  
pp. 101-106
Author(s):  
Akira Ishikawa ◽  
Kazuya Kuraoka ◽  
Junichi Zaitsu ◽  
Akihisa Saito ◽  
Atsushi Kamigaichi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Case Report ◽  
Literature Review ◽  
Tumor Cells ◽  
Growth Pattern ◽  
Mediastinal Mass ◽  
Smooth Muscle Actin ◽  
Anterior Mediastinum ◽  
Anterior Mediastinal Mass ◽  
Muscle Actin

Primary mediastinal sarcomas are extremely rare. Additionally, mediastinal leiomyosarcomas account for approximately 9% of mediastinal sarcoma cases. Until date, only few cases of anterior mediastinal leiomyosarcomas have been reported. Herein, we report a case of an 85-year-old female with an anterior mediastinal mass of 15 mm. Histological examination revealed spindle tumor cells showing a fascicular growth pattern. Immunohistochemically, the tumor cells were focal positive for desmin, calponin, and α-smooth muscle actin. The pathological diagnosis was leiomyosarcoma. In conclusion, we encountered a case of a very rare leiomyosarcoma that occurred in the anterior mediastinum, and our report may contribute to the understanding of this disease.

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