scholarly journals Complete mitogenome of Anopheles sinensis and mitochondrial insertion segments in the nuclear genomes of 19 mosquito species

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0204667 ◽  
Author(s):  
Yi-Ran Ding ◽  
Bo Li ◽  
Yu-Juan Zhang ◽  
Qi-Meng Mao ◽  
Bin Chen
Keyword(s):  
Mosquito Species ◽  
Anopheles Sinensis ◽  
Complete Mitogenome ◽  
Nuclear Genomes

scholarly journals First molecular detection of Plasmodium relictum in Anopheles sinensis and Armigeres subalbatus

2020 ◽  
Vol 10 (1) ◽  
pp. 39-43
Author(s):  
Jilei Zhang ◽  
Guangwu Lu ◽  
Patrick Kelly ◽  
Jing Li ◽  
Min Li ◽  
...  
Keyword(s):  
Avian Malaria ◽  
Mosquito Species ◽  
Resonance Energy Transfer ◽  
Plasmodium Species ◽  
Resonance Energy ◽  
Anopheles Sinensis ◽  
Plasmodium Relictum ◽  
Armigeres Subalbatus ◽  
Natural Vector ◽  
Pipiens Pallens

Background: Plasmodium relictum is one of the most important avian malaria species, which is mainly seen in wild birds, with infections reported in more than 70 different species and at high prevalence.Aim: The aim of this study was to determine the molecular prevalence of Plasmodium spp. in mosquitoes collected in China.Method: A Plasmodium -specific fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) was established in this study to analyze five species of mosquitoes (1,620 Culex pipiens pallens, 806 Aedes albopictus, 377 Armigeres subalbatus, 168 Anopheles sinensis, and 80 Culex tritaeniorhynchus) collected in hand nets from homes in 25 provinces of China.Results: Only females originated from six provinces were determined to be positive (0.6%, 10/1,809). Plasmodium species were detected in three mosquito species, such as C. pipiens pallens (0.5%, 8/1,620), A. sinensis (0.6%, 1/168), and A. subalbatus (0.3%, 1/377). Of the three mosquito species positive for P. relictum, only C. pipiens pallens is known to feed on birds and is recognized as the natural vector of P. relictum.Conclusion: This is the first time that P. relictum has been detected in A. sinensis and A. subalbatus. P. relictum, the agent of avian malaria, was present in mosquitoes in China, including mosquito species not previously thought to be the vectors. Keywords: Anopheles sinensis, Armigeres subalbatus, China, Mosquito, Plasmodium relictum.

scholarly journals Metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the Shaanxi-Gansu-Ningxia region, China

2021 ◽  
Vol 15 (4) ◽  
pp. e0009381
Author(s):  
Xiaozhou He ◽  
Qikai Yin ◽  
Liwei Zhou ◽  
Lei Meng ◽  
Weijun Hu ◽  
...  
Keyword(s):  
Culex Pipiens ◽  
High Throughput Sequencing ◽  
Mosquito Species ◽  
Gansu Province ◽  
Anopheles Sinensis ◽  
Northwestern China ◽  
Shaanxi Province ◽  
Viral Diversity ◽  
Metagenomic Sequencing ◽  
Culex Tritaeniorhynchus

Background Mosquitoes host and transmit numerous arthropod-borne viruses (arboviruses) that cause disease in both humans and animals. Effective surveillance of virome profiles in mosquitoes is vital to the prevention and control of mosquito-borne diseases in northwestern China, where epidemics occur frequently. Methods Mosquitoes were collected in the Shaanxi-Gansu-Ningxia region (Shaanxi Province, Gansu Province, and Ningxia Hui Autonomous Region) of China from June to August 2019. Morphological methods were used for taxonomic identification of mosquito species. High-throughput sequencing and metagenomic analysis were used to characterize mosquito viromes. Results A total of 22,959 mosquitoes were collected, including Culex pipiens (45.7%), Culex tritaeniorhynchus (40.6%), Anopheles sinensis (8.4%), Aedes (5.2%), and Armigeres subalbatus (0.1%). In total, 3,014,183 (0.95% of clean reads) viral sequences were identified and assigned to 116 viral species (including pathogens such as Japanese encephalitis virus and Getah virus) in 31 viral families, including Flaviviridae, Togaviridae, Phasmaviridae, Phenuiviridae, and some unclassified viruses. Mosquitoes collected in July (86 species in 26 families) showed greater viral diversity than those from June and August. Culex pipiens (69 species in 25 families) and Culex tritaeniorhynchus (73 species in 24 families) carried more viral species than Anopheles sinensis (50 species in 19 families) or Aedes (38 species in 20 families) mosquitoes. Conclusion Viral diversity and abundance were affected by mosquito species and collection time. The present study elucidates the virome compositions of various mosquito species in northwestern China, improving the understanding of virus transmission dynamics for comparison with those of disease outbreaks.

scholarly journals Presence of entomobirnaviruses in Chinese mosquitoes in the absence of Dengue virus co‐infection

2013 ◽  
Vol 94 (3) ◽  
pp. 663-667 ◽  
Author(s):  
Yong Huang ◽  
Zhiqiang Mi ◽  
Lu Zhuang ◽  
Maijuan Ma ◽  
Xiaoping An ◽  
...  
Keyword(s):  
Dengue Virus ◽  
High Throughput ◽  
South China ◽  
Small Rnas ◽  
High Throughput Sequencing ◽  
Mosquito Species ◽  
Anopheles Sinensis ◽  
Insect Vectors ◽  
Drosophila X Virus ◽  
Espirito Santo

Birnaviruses, including the genus Entomobirnavirus, are socio-economically important viruses. Currently, only Drosophila X virus has been formally assigned to the genus Entomobirnavirus, but two more viruses were recently isolated, Espirito Santo virus (ESV) and Culex Y virus. The host mosquito has been reported to carry many viruses, but seldom entomobirnaviruses. To discover potential pathogens in mosquitoes, we exploited small-RNAs high-throughput sequencing of three mosquito species caught in South China. A virus that genetically likes entomobirnavirus, Mosquito X virus (MXV), was identified from Anopheles sinensis and was 97 % identical to ESV, which co-infects with Dengue virus (DENV). However, the absence of DENV in the A. sinensis suggested the independence of MXV infection from dengue co-infection. Our discovery complements prior research on entomobirnaviruses and proved that MXV may be widespread in mosquitoes on different continents. This work also highlights the applying of high-throughput sequencing of small RNAs to survey viruses carried by insect vectors.

scholarly journals Complete Circularized Genome Sequences of Four Strains of Elizabethkingia anophelis, Including Two Novel Strains Isolated from Wild-Caught Anopheles sinensis

2017 ◽  
Vol 5 (47) ◽  
Author(s):  
Dong Pei ◽  
Ainsley C. Nicholson ◽  
Jinjin Jiang ◽  
Huiying Chen ◽  
Anne M. Whitney ◽  
...  
Keyword(s):  
Genetic Similarity ◽  
Mosquito Species ◽  
Public Domain ◽  
Anopheles Sinensis ◽  
Genome Sequences ◽  
Content Type ◽  
The Public

ABSTRACT We provide complete circularized genome sequences of two mosquito-derived Elizabethkingia anophelis strains with draft sequences currently in the public domain (R26 and Ag1), and two novel E. anophelis strains derived from a different mosquito species, Anopheles sinensis (AR4-6 and AR6-8). The genetic similarity of all four mosquito-derived strains is remarkable.

The Scientific Basis for the Prevention of Zooanthroponoze Vector-Borne Diseases Spread by Parasitic Arthropods of the Center of the East European Plain

2020 ◽  
Vol 14 (1) ◽  
pp. 81-88
Author(s):  
Fedor I. Vasilevich ◽  
Anna M. Nikanorova
Keyword(s):  
Mathematical Models ◽  
Culex Pipiens ◽  
Preventive Measures ◽  
Mosquito Species ◽  
Natural Habitat ◽  
Piperonyl Butoxide ◽  
East European Plain ◽  
Vector Borne Diseases ◽  
Vector Borne ◽  
Kaluga Region

The purpose of the research is development of preventive measures against zooanthroponoze vector-borne diseases spread by parasitic arthropods in the Kaluga Region. Materials and methods. The subject of the research was Ixodidae, mosquitoes, and small mammals inhabiting the Kaluga Region. The census of parasitic arthropods was carried out on the territory of all districts of the Kaluga Region and the city of Kaluga. Open natural habitat and human settlements were investigated. Weather conditions from 2013 to 2018 were also taken into account. For the purposes of the study, we used standard methods for capturing and counting arthropods and mouse-like rodents. In order to obtain mathematical models of small mammal populations, a full factorial experiment was conducted using the collected statistical data. In-process testing of the drug based on s-fenvalerate and piperonyl butoxide were carried out under the conditions of the agricultural collective farm “Niva” of the Kozelsky District, the Kaluga Region, and LLC “Angus Center of Genetics” of the Babyninsky District, the Kaluga Region. Results and discussion. In the Kaluga Region, two species of ixodic ticks are found, namely, Ixodes ricinus and Dermacentor reticulatus, which have two activity peaks. Mosquito may have 3-4 generations in a year in the Kaluga region. The most common mosquito species in the Kaluga Region are Aedes communis, Ae. (Och.) togoi and Ae. (Och.) diantaeus, Culex pipiens Culex Linnaeus, 1758 (Diptera, Culicidae) (Culex pipiens): Cx. pipiens f. pipiens L. (non-autogenic form) and Cx. p. f. molestus Fors. (autogenic form), which interbreed, and reproductively isolated in the Region. The developed mathematical models make it possible to quantify the risks of outbreaks of zooanthroponoze vector-borne diseases without the cost of field research, and allow for rational, timely and effective preventive measures. Medications based on s-fenvalerate and piperonyl butoxide and based on cyfluthrin showed high insecto-acaricidal efficacy and safety.

A Simple PCR Assay for Discrimination of Dengue Vectors from Nsukka LGA, Nigeria

2020 ◽  
Vol 10 (1) ◽  
pp. 67-77
Author(s):  
Amos Watentena ◽  
Ikem Chris Okoye ◽  
Ikechukwu Eugene Onah ◽  
Onwude Cosmas Ogbonnaya ◽  
Emmanuel Ogudu
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Mosquito Species ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Mosquito Vector ◽  
Aedes Mosquitoes ◽  
Allele Specific ◽  
Arboviral Diseases ◽  
Dengue Vectors

Mosquitoes of Aedes species are vectors of several arboviral diseases which continue to be a major public health problem in Nigeria. This study among other things, morphologically identified Aedes mosquitoes collected from Nsukka LGA and used an allele specific PCR amplification for discrimination of dengue vectors. Larval sampling, BG-sentinel traps and modified human landing catches were used for mosquito sampling in two selected autonomous communities of Nsukka LGA (Nsukka and Obimo). A total of 124 Aedes mosquitoes consisting of five (5) different species were collected from April to June, 2019 in a cross-sectional study that covered 126 households, under 76 distinct geographical coordinates. Larvae was mainly collected from plastic containers 73% (n=224), metallic containers 14% (n=43), earthen pots 9% (n=29) and used car tyres 3% (n=9), reared to adult stage 69.35% (n=86), and all mosquitoes were identified using standard morphological keys. Five (5) Aedes mosquito species were captured; Aedes aegypti 83(66.94%), Aedes albopictus 33(26.61%), Aedes simpsoni (4.48%), Aedes luteocephalus (≤1%) and Aedes vittatus (≤1%). Nsukka autonomous community had higher species diversity than Obimo. Allele specific amplification confirmed dengue vectors, Aedes aegypti and Aedes albopictus species on a 2% agarose gel. Since the most recent re-emergence of arboviral diseases is closely associated with Aedes species, findings of this study, therefore, give further evidence about the presence of potential arboviral vectors in Nigeria and describe the role of a simple PCR in discriminating some. Further entomological studies should integrate PCR assays in mosquito vector surveillance.

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