scholarly journals Clonal diversity and spatial dissemination of multi-antibiotics resistant Staphylococcus aureus pathotypes in Southwest Nigeria

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247013
Author(s):  
Akinniyi Paul Akinduti ◽  
Joshua Adekunle Osiyemi ◽  
Temitope Temitayo Banjo ◽  
Oluwaseun Ejilude ◽  
Maged El-Ashker ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Infection Control ◽  
Resistance Index ◽  
Clonal Diversity ◽  
Female Gender ◽  
Resistance Rate ◽  
Accessory Gene ◽  
Southwest Nigeria ◽  
Amoxicillin Clavulanate

Spread of genetically diverse Staphylococcus aureus characterized with multi-antibiotic resistance and regulated by high level agr functionalities in several communities in southwest Nigeria was investigated and evaluated for infection control. Staphylococcus aureus pathotypes recovered from 256 cases including purulent pus from skin infections, soft tissue aspirates, wounds, otorrhea, eye, throat and endocervical infections were assayed for biofilm and antibiogram. Further genotyped with micro-array, mapped for geospatial distribution and evaluated for clonal diversity and functional accessory gene regulators (agr). Significant Staphylococci infection among the ages (OR:0.021, CI:0.545–1.914) and female gender with prevalence rate of MSSA (53.0%) and MRSA (1.5%) (OR:1.021, CI:0.374–1.785) were observed. More than 52.5% resistance rates to tetracycline and amoxicillin with significant median resistance were observed in all the infection cases (p = 0.001). Resistance rate of 78.8% at MIC50 32μg/ml and MIC90 128μg/ml to amoxicillin-clavulanate, and more than 40% resistance to ceftazidime, ciprofloxacin and tetracycline of MIC90 and MIC50 at 32 μg/ml were observed. Strains with multi-antibiotic resistance index above 0.83, high beta-lactamase and strong biofilm clustered into separate phylo-group. Heterogeneous t442 (wound and pus), t657 (wound), t091 (ear) and t657 (ear and wound) revealed high phylogenetic diversity. Only 4.6% pvl+ MSSA-CC1 agrI, pvl+ MSSA-CC5 (13.6%) and pvl+ MRSA-CC7 agrII (4.6%), expressed enterotoxin, leukocidins, proteases and resistance gene determinants. Livestock clonal types clustered with identified community-associated strains. Clonal dissemination of resistant pvl+ MSSA-CC1 and MRSA-CC5 encoding agr were predominant in several peri-urban communities where adequate geno-surveillance, population-target antimicrobial stewardship, extensive community structured infection control programs are needed to prevent further focal dissemination.

scholarly journals Infection Control of Spatial Disseminated Multi-Antibiotics Resistant And Phylo-Diverse Staphylococcus Aureus Pathotypes

2020 ◽  
Author(s):  
PAUL AKINDUTI ◽  
Joshua Osiyemi ◽  
Temitope Banjo ◽  
Oluwaseun Ejilude ◽  
Maged Ashker ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Infection Control ◽  
Urban Communities ◽  
Bovine Milk ◽  
Resistance Index ◽  
Resistance Rate ◽  
High Rate ◽  
Accessory Gene ◽  
Resistance Rates

Abstract Focal dissemination of multi-antibiotic resistant (MAR) Staphylococci pathotypes regulated by agr functionalities was investigated and evaluated for infection control. Non-repetitive Staphylococcus aureus strains from soft and skin infections disseminated in several communities were recovered and biotyped, assayed for biofilm and profiled for antibiotic resistance. Strains were further genotyped for spa types, virulence and resistant genes; and mapped for geospatial distribution. Clonal diversity and functional accessory gene regulators (agr) were also evaluated. Staphylococcal infection was not significant with age group (p>0.05), but high rate of MSSA (53.0%) and MRSA (1.5%) was observed. Median resistance rates were significantly differ (p=0.001) but highest 75 th percentile and media resistance rates were observed in wound infection. Resistance rate of 78.8% at MIC 50 32µg/ml and MIC 90 128µg/ml to amoxicillin-clavulanate, and more than 40% resistance to ceftazidime, ciprofloxacin, gentamycin, ofloxacin, sulfamethoxazole and tetracycline with MIC 90 and MIC 50 at 32 µg/ml were observed. More than 0.83 multi-antibiotic resistance index (MARI) were observed among the strains that clustered into separate phylo-group expressing high beta- lactamase and strong biofilm production. Heterogeneous spa types t442 (wound and pus), t657 (wound), t091 (ear) and t657 (ear and wound) revealed high phylo- diversity. Only 4.6% pvl + MSSA-CC1 agr I, pvl+ MSSA-CC5 (13.6%) and pvl+ MRSA-CC7 agr II (4.6%), expressed enterotoxin; sea, sec, sed, sej, Leukocidins ( LukF-PV, lukD, lukE ), proteases (aur, slpA sspB, sspE ) and resistance genes (fosB, msr (A), bla mph(C),aphA3, sat, fosB, sdrM, Q7A4X2) . Phylogenetic related spa types of livestock origin, specifically bovine milk clustered with detected strains that were prevalent in urban communities with focal dissemination to other nearest suburbs. Clonal dissemination resistant pvl+MAR MSSA-CC1 and MRSA- CC5 encoding agr were predominant in several peri-urban communities. This require adequate geno-surveillance, population-target antimicrobial stewardship, extensive community health care intervention policy and well-structured infection control programs to prevent further focal dissemination.

scholarly journals Clonal diversity and genomic characterization of Panton-valentine Leukocidin (PVL)-positive Staphylococcus aureus in Tehran, Iran

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zahra Najafi olya ◽  
Shahin Najar-Peerayeh ◽  
Abbas Yadegar ◽  
Bita Bakhshi
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Epidemiology ◽  
Clonal Diversity ◽  
Resistance Rate ◽  
Type I ◽  
Limited Information ◽  
Accessory Gene ◽  
Mrsa Strains ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Abstract Background Some Staphylococcus aureus strains produce Panton-Valentine leukocidin (PVL), a bi-component pore-forming toxin, which causes leukocyte lysis and tissue necrosis. Currently, there is very limited information on the molecular epidemiology of PVL-encoding S. aureus strains in Iran. This study aimed to determine the molecular epidemiology and genetic background of PVL-positive S. aureus clinical strains isolated from Iranian patients. Methods A total of 28 PVL-positive S. aureus strains were detected from 600 S. aureus isolates between February 2015 and March 2018 from different hospitals in Tehran, Iran. Antimicrobial susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Molecular genotyping was performed using SCCmec and accessory gene regulator (agr) typing, PVL haplotyping, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). Results The highest antibiotic resistance rate was found to be against erythromycin (57.1%), followed by ciprofloxacin (42.8%) and clindamycin (35.7%). Moreover, 19 (67.9%) out of 28 S. aureus isolates were identified as MRSA, including CA-MRSA (14/19, 73.7%) and HA-MRSA (5/19, 26.3%). SCCmec type IVa was detected as the predominant type (10/19, 52.6%), followed by type III (5/19, 26.3%) and type V (4/19, 21.1%). The agr type I was identified as the most common type (14/28, 50%), and H and R haplotype groups were observed at frequencies of 67.9 and 32.1%, respectively. Among H variants, the predominant variant was H2 (78/9%). The isolates encompassed 21 different sequence types (STs), including 16 new STs (ST5147 to ST5162). Based on eBURST analysis, the isolates were clustered into five CCs, including CC30, CC22, CC1, CC8, and CC5 (ST5160), and nine singletons. PFGE typing showed that 24 isolates were clustered into A (4 pulsotypes), B (9 pulsotypes), and C (11 pulsotypes) clusters. Conclusions A high prevalence of PVL-positive CA-MRSA strains was detected in Iran. The majority of PVL-positive isolates were of H (mostly H2) variant, while R variant was harbored by 100% of PVL-positive MRSA strains. Also, CC8, CC22, and CC30 were identified as the dominant clones among PVL-encoding S. aureus strains. This study promotes a better understanding of the molecular epidemiology and evolution of PVL-positive S. aureus strains in Iran.

scholarly journals Methicillin-resistant Staphylococcus aureus of the clonal lineage ST5-SCCmecII-t2460 was associated with high mortality in a Wuhan hospital

2021 ◽  
Author(s):  
Xuehan Li ◽  
Jing Zhang ◽  
Yifan Zhang ◽  
Junying Zhou ◽  
Xinwei Li ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
White Blood Cells ◽  
Resistance Rate ◽  
Molecular Characteristics ◽  
Invasive Procedures ◽  
Methicillin Resistant ◽  
Small Colony Variant ◽  
Recent Emergence ◽  
Resistance Rates

AbstractMethicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen that can cause serious infectious diseases. An emerging MRSA strain, ST5-SCCmecII spa-type-t2460 (SMRSA), has spread rapidly since its recent emergence in China, but little information is available about this lineage. In this study, 91 MRSA isolates were collected from patients treated in the Zhongnan Hospital, Wuhan University, from 2018 to 2019, and investigated for their molecular characteristics, antibiotic resistance profiles, and clinical characteristics. The predominant lineage, SMRSA, accounted for 37.4% (34/91) of the isolates, followed by ST239-SCCmecIII-t030 (19.8%, 18/91) and ST59-SCCmecIV-t437 (8.8%, 8/91). In contrast to the latter two non-SMRSA (nSMRSA) lineages, which are among the main MRSA found in Chinese settings, SMRSA exhibited small colony variant (SCV) phenotype and had extremely high resistance rates to erythromycin (100.0%), clindamycin (100.0%), levofloxacin (100.0%), tetracycline (97.1%), moxifloxacin (97.1%), and ciprofloxacin (100%), but was more susceptible to rifampicin (resistance rate 3%). The levels of white blood cells (WBC) and procalcitonin (PCT) and the 30-day mortality in patients infected with SMRSA were (12.54 ± 6.61) × 109/L, 0.66 ng/mL, and 52.9%, respectively, which were much higher than those in patients infected with nSMRSA. In addition, patients infected with SMRSA were more frequently admitted to the intensive care unit (ICU) and submitted to invasive procedures than those infected with nSMRSA. In conclusion, SMRSA showed SCV phenotype and exhibited multiple antibiotic-resistance profiles. In this study, SMRSA was associated with serious infections and poor prognosis. Compared with ST239, ST59, or other nSMRSA strains, patients infected with SMRSA strains have higher 30-day mortality, increased levels of inflammatory biomarkers, and more frequent ICU hospitalization and invasive procedures.

scholarly journals Antibiotic resistance and clonal diversity of invasive Staphylococcus aureus in the rural Ashanti Region, Ghana

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Denise Dekker ◽  
Manuel Wolters ◽  
Eva Mertens ◽  
Kennedy Gyau Boahen ◽  
Ralf Krumkamp ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Clonal Diversity ◽  
Ashanti Region

scholarly journals Clonality, virulence genes, and antibiotic resistance of Staphylococcus aureus isolated from blood in Shandong, China

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xuezhi Wang ◽  
Dongzi Lin ◽  
Zengqi Huang ◽  
Jinmei Zhang ◽  
Wenyan Xie ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Infection Control ◽  
Virulence Genes ◽  
Genetic Relatedness ◽  
Protein A ◽  
Epidemiological Data ◽  
Control Measures ◽  
Staphylococcal Protein A ◽  
Infection Control Measures

Abstract Background Bloodstream infection (BSI) caused by Staphylococcus aureus (S. aureus) can be life-threatening and pose a great challenge to infection control and clinical treatment. However, little information exists regarding the characterization of S. aureus in BSI patients in Shandong, China. To identify the clonality, virulence genes, and antibiotic resistance of S. aureus in blood, a total of 101 nonrepetitive blood isolates were collected. The antibiotic resistance phenotypes were determined, and virulence genes were analyzed with polymerase chain reaction (PCR). Finally, the genetic relatedness was investigated with Staphylococcus chromosomal cassette mec (SCCmec) typing for methicillin-resistant S. aureus (MRSA) isolates, Staphylococcal protein A (spa), and multilocus sequence typing (MLST) for all of 101 isolates. Results Of the 101 S. aureus isolates, 24 MRSA isolates and 77 methicillin-susceptible S. aureus (MSSA) isolates were identified. Overall, MRSA isolates had higher resistance rates than MSSA isolates when exposed to any of the 15 antibiotics tested in this study except for trimethoprim/sulfamethoxazole. Among the 17 virulence genes tested in this study, hla, hld, and hlg could be detected in all isolates. MRSA isolates were more likely to carry seb and hlb genes, while MSSA isolates were more likely to carry seg and sei genes. Thirty-five sequence types (STs) and 49 spa types were identified, of which ST59-t437 and ST398-t571 were the most abundant. These two genotypes were also the most abundant ST-spa types in MRSA and MSSA isolates, but their abundances shifted over time, with ST398-t571 being the predominant genotype from 2016 to 2017, and ST59-t437 from 2018 to 2020. Besides, all the ST59-t437 isolates harbored hlgb gene, whereas most (88.9%) ST398-t571 did not. In addition, twenty-four MRSA isolates were subject to SCCmec typing. SCCmec IVa was the most prevalent SCCmec type, and all the ST59-t437 MRSA isolates were SCCmec IVa. We also observed 15 new STs, and some of them were MRSA. Conclusion These findings provide additional observations and epidemiological data for blood S. aureus isolates, which can improve future infection-control measures and aid in potential clinical treatments in hospitals and other clinical settings.

scholarly journals Antibiotic Resistance Pattern of Staphylococcus Aureus Isolated From Pediatrics With Ocular Infections: A 6-Year Hospital-Based Study in China

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiao-Yu Zheng ◽  
Bonnie Nga Kwan Choy ◽  
Ming-Ming Zhou ◽  
Zheng-Yan Zhao
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Resistance Rate ◽  
Resistance Pattern ◽  
Ocular Infection ◽  
Ocular Infections ◽  
Resistance Patterns ◽  
Mrsa Infection ◽  
Resistance Rates ◽  
Antibiotic Resistance Patterns

Staphylococcus aureus (S. aureus) is an important pathogen of ocular infections in pediatrics. The study aimed to identify the prevalence and resistance pattern of S. aureus, especially methicillin-resistant S. aureus (MRSA), in Chinese children with ocular infections. All patients with S. aureus infections were reviewed at a tertiary children's hospital during 2015–2020, and those with ocular infections were investigated for susceptibility results. Of 1,668 S. aureus strains, there were 177 unique isolates from ocular infection. Among them, 45 (25.4%) were MRSA and 132 (74.6%) were methicillin-sensitive S. aureus (MSSA). The proportion of MRSA did not change over time. Most of the strains were obtained from the neonate ward and ophthalmology department (n = 88, 49.7%, and n = 85, 48.0%, respectively), while eye secretion and pus were the main specimen types (n = 128, 72.3%, and n = 37, 20.9%, respectively). MRSA was significantly resistant against penicillin class (97.8%), erythromycin (71.1%), clindamycin (71.1%), and tetracycline (32.1%), with a high multidrug resistance (MDR) rate of 71.1%. However, MRSA was highly sensitive to levofloxacin. Resistance rates against erythromycin and ciprofloxacin as well as MDR percentage all increased among MSSA in children above 1 year of age, ophthalmology department, and outpatient population and decreased in eye secretion specimen. The mean resistance percentage remained stable for MRSA and MSSA during the study period. The survey of ocular S. aureus pathogens in pediatrics and their antibiotic resistance patterns helps in clinical treatment. MRSA with many strains demonstrating MDR is highly prevalent in children with ocular infections in Southeast China. Levofloxacin is an effective topical antibiotic for ocular MRSA infection, while erythromycin has a high resistance rate. The antibiotic resistance patterns of MRSA and MSSA differs and varies by different stratifications. A cautious use of antibiotics should be considered.

scholarly journals Geospatial Investigation of Nigerian Honey and Detection of Anti-Enteric Biomarker

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
P. Akinniyi Akinduti ◽  
Oluwaseun Ejilude ◽  
Joseph Olugbuyiro ◽  
Adeyemi G. Adewale ◽  
Okanlawon Onagbesan ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Antibiotic Resistance ◽  
Resistance Index ◽  
Antibiotic Resistant ◽  
Faecal Samples ◽  
Southwest Nigeria ◽  
R Plasmids ◽  
Time Kill ◽  
Geographical Locations ◽  
Geospatial Mapping

Geospatial mapping and antibacterial biomarkers were investigated in Nigerian honey used for therapeutic purposes in several communities affected with prevalent antibiotic-resistant enteric bacilli. Randomly collected enteric bacilli from faecal samples were biotyped and phenotypically assayed for antibiotic resistance and profiled for R plasmids. R plasmid molecular weight and multiantibiotic resistance index (MARI) relatedness were evaluated for resistance among phylogroups. Honey cidal activity, time kill kinetics, and bioactive markers were determined and analysed for geospatial distribution. More than 30% enteric biotypes were resistant to cotrimoxazole, ciprofloxacin, and tetracycline at MIC ≥16 μg/ml (P=0.004). Two unrelated cluster complexes with diverse antibiotic resistance indices expressed high molecular weight plasmid (14.17 kbp) with 0.73 MARI to two classes of antibiotics. Among the resistant bacilli, only 24.3% (MIC90 500 mg/mL) and 8.1% (MBC90 1000 mg/mL) were susceptible to honey with evidence of 14.85% and 5.94% significant viable reduction at 2 × MIC to less than 2.50 Log10 CFU/mL (P<0.05). Only alkaloids significantly regressed (P=0.028) with susceptibility of resistant bacilli significantly correlate with bacteria inhibition (r = 0.534, P=0.049) at optimal cutoff limit of 0.32 mg/ml. Antibacterial honey with significant alkaloid biomarkers was detected at 3°10′0–3°30′0E and 6°30′0–7°30′0N of Southwest Nigeria. Spatial mapping evidently indicated variation in honey physicochemical and bioactive compounds and identified geographical locations suitable for production of anti-enteric honey rich in alkaloids marker required for prevention and treatment of resistant enteric bacilli infections.

scholarly journals Nasal Carriage and Antibiotic Resistance Pattern of Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Staff of a Referral Hospital, Zabol, Iran

2019 ◽  
Vol 4 (2) ◽  
pp. 81-85
Author(s):  
Mina Mir ◽  
Jalileh Kordi ◽  
Zahra Rashki Ghalehnoo ◽  
Omid Tadjrobehkar ◽  
Hamid Vaez
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Nasal Carriage ◽  
Resistance Rate ◽  
Referral Hospital ◽  
Resistance Pattern ◽  
Anatomical Site ◽  
Methicillin Resistant ◽  
Antibiotic Resistance Profile ◽  
Resistance Patterns

Introduction: Staphylococcus aureus is known as the causative agent of various infections in humans, and the nasal cavity is the main anatomical site for storing and spreading this pathogen in health care workers (HCWs) in hospital settings. Methicillin-resistant S. aureus (MRSA) strains are resistant to many antibiotics. The aim of this study was to investigate the prevalence and antibiotic resistance patterns of S. aureus strains isolated from the nasal carriage of HCWs in a referral hospital in Zabol. Methods: A total of 277 HCWs of Amir-Al-Momenin hospital of Zabol participated voluntarily in this study from March to September 2017. Demographic information was collected using questionnaire. Bacterial isolates were collected from anterior nasal canal using cotton swabs. MRSA isolates were detected by Oxacillin Screen agar and polymerase chain reaction (PCR). Antibiotic resistance was assessed by the disk-diffusion method.Results: In total, 10.8% of HCWs were carriers of S. aureus and 46.7% of the isolates were found to be MRSA. Laboratory staffs were the most frequently colonized HCWs. All the isolates were susceptible to vancomycin, teicoplanin and gentamicin. The highest resistance rate (64.3%) of MRSA isolates was observed against erythromycin.Conclusion: Our findings showed that gentamycin and rifampin might be useful to eradicate S.aureus. Regular screening of HCWs and assessment of antibiotic resistance profile are essential to prevent MRSA dissemination in hospitals.

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