scholarly journals Sero-surveillance for SARS-CoV-2 infection among healthcare providers in four hospitals in Thailand one year after the first community outbreak

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254563
Author(s):  
Wanitchaya Kittikraisak ◽  
Phunlerd Piyaraj ◽  
Apichat Vachiraphan ◽  
Thanapat Wongrapee ◽  
Somsak Punjasamanvong ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Healthcare Providers ◽  
Igg Antibodies ◽  
Imported Case ◽  
Immunosorbent Assays ◽  
Blood Specimens ◽  
One Year ◽  
Community Outbreaks ◽  
Spike Proteins

Background Thailand was the first country outside China to report SARS-CoV-2 infected cases. Since the detection of the first imported case on January 12th, 2020 to the time this report was written, Thailand experienced two waves of community outbreaks (March-April 2020 and December 2020-March 2021). We examined prevalence of SARS-CoV-2 seropositivity among healthcare providers (HCPs) in four hospitals approximately one year after SARS-CoV-2 first detected in Thailand. By March 2021, these hospitals have treated a total of 709 coronavirus disease 2019 (COVID-19) patients. Methods Blood specimens, collected from COVID-19 unvaccinated HCPs during January-March 2021, were tested for the presence of SARS-CoV-2 immunoglobulin G (IgG) antibodies to nucleocapsid (IgG-nucleocapsid) and spike (IgG-spike) proteins using Euroimmune® enzyme-linked immunosorbent assays. Results Of 600 HCPs enrolled, 1 (0.2%) tested positive for the SARS-CoV-2 IgG-spike antibodies, but not the IgG-nucleocapsid. Conclusion The presence of SARS-CoV-2 IgG antibodies was rare in this sample of HCPs, suggesting that this population remains susceptible to SARS-CoV-2 infection.

scholarly journals Pertussis surveillance by small serosurveys of blood donors

2018 ◽  
Vol 146 (14) ◽  
pp. 1807-1810 ◽  
Author(s):  
N. Kennerknecht ◽  
M. Riffelmann ◽  
U. Voelker ◽  
C. H. Wirsing von König
Keyword(s):  
Pertussis Toxin ◽  
Bordetella Pertussis ◽  
Immunoglobulin G ◽  
Blood Donors ◽  
Igg Antibodies ◽  
Cheap Method ◽  
Immunosorbent Assays ◽  
Observation Period

AbstractSerosurveys have established data about the distribution of immunoglobulin G (IgG)-antibodies to pertussis toxin (PT) in various populations. We tried to detect whether small serosurveys in blood donors could serve as a simple and inexpensive means to collect information about the circulation ofBordetella pertussis. We screened every donation in 307 adult blood donors aged 19–69 years for IgG-anti-PT by standardised enzyme-linked immunosorbent assays (ELISA), and the donors were followed between 2014 and 2016 for a total of 426 person-years. When we used a vertical survey with cut-offs of 100, 62.5 and 40 IU/ml, respectively, as an indicator for recent contacts withB. pertussis, nine (2.9%), 22 (7.2%) and 54 (17.6%) of donors had IgG-anti-PT titres above the respective levels. During the horizontal observation period of 426 person years, six significant increases and two conversions were found, which lead to an estimate of 1878 contacts/100.000 person-years (1.9% per year). Median and mean IgG-anti-PT concentrations remained relatively stable from year to year during the observation period. Our findings show that small serosurveys of blood donors offer a simple and cheap method for the surveillance ofB. pertussis.

scholarly journals Comparison of Five Commercial Anti-Tetanus Toxoid Immunoglobulin G Enzyme-Linked Immunosorbent Assays

2009 ◽  
Vol 16 (12) ◽  
pp. 1837-1839 ◽  
Author(s):  
A. L. Perry ◽  
A. J. Hayes ◽  
H. A. Cox ◽  
F. Alcock ◽  
A. R. Parker
Keyword(s):  
Tetanus Toxoid ◽  
Immunoglobulin G ◽  
Igg Antibodies ◽  
Immunosorbent Assays

ABSTRACT Five commercially available enzyme-linked immunosorbent assays for the measurement of anti-tetanus toxoid immunoglobulin G (IgG) antibodies were evaluated for performance. The data suggest that there are manufacturer-dependent differences in sensitivity and accuracy for the determination of tetanus toxoid IgG antibodies that could result in different diagnostic interpretations.

scholarly journals Comparison of Three Enzyme-Linked Immunosorbent Assays for Detection of Immunoglobulin G Antibodies to Tetanus Toxoid with Reference Standards and the Impact on Clinical Practice

2008 ◽  
Vol 15 (12) ◽  
pp. 1751-1754 ◽  
Author(s):  
Karen H. van Hoeven ◽  
Connie Dale ◽  
Phil Foster ◽  
Barbara Body
Keyword(s):  
Binding Site ◽  
Tetanus Toxoid ◽  
Immunoglobulin G ◽  
Accurate Determination ◽  
Reference Standards ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Igg Antibody ◽  
Immunosorbent Assays ◽  
The Impact

ABSTRACT Accurate determination of the concentrations of immunoglobulin G (IgG) antibody to tetanus toxoid is important in order to evaluate the immunogenicity of tetanus toxoid vaccines, determine immune competence in individual patients, and measure the prevalence of immunity in populations. The performance of three commercially available enzyme-linked immunosorbent assays (ELISAs) for IgG antibodies to tetanus toxoid were evaluated. Serially diluted NIBSC 76/589 and TE-3 human tetanus IgG immunoglobulin international reference standards were analyzed in quadruplicate using ELISAs manufactured by The Binding Site, Inc. (VaccZyme); Scimedx; and Euroimmun. In addition, IgG antibodies to tetanus toxoid were measured in 83 deidentified serum specimens using each manufacturer's ELISA. Each ELISA provided linear results when evaluated with the reference preparations. The Binding Site ELISA provided results that closely corresponded to the reference preparations (y = 1.09x − 0.08), whereas the Scimedx ELISA gave results that were consistently lower (y = 0.21x − 0.07) and the Euroimmun ELISA gave results that were consistently higher (y = 1.5x + 0.30) than the reference preparation concentrations. Using the recommended cutoff for each ELISA (<0.10 IU/ml), the overall agreement of all of the ELISA methods was 78%. Three of eighty-three (3.6%) human serum samples demonstrated inadequate immunity with all three assays. The Binding Site ELISA yielded nonprotective antibody concentrations in only these 3 samples, whereas 19 samples (22.9%) according to the Scimedx ELISA and 6 samples (7.2%) according to the Euroimmun ELISA demonstrated nonprotective concentrations. The performance characteristics of ELISAs for tetanus immunoglobulin titers were manufacturer dependent, and the differences translated into important disparities in reported results.

scholarly journals Changes in Cytomegalovirus Seroprevalence Among U.S. Children Aged 1–5 Years: The National Health and Nutrition Examination Surveys

2020 ◽  
Author(s):  
Molly R Petersen ◽  
Eshan U Patel ◽  
Alison G Abraham ◽  
Thomas C Quinn ◽  
Aaron A R Tobian
Keyword(s):  
Confidence Interval ◽  
National Health ◽  
Immunoglobulin G ◽  
Igg Antibodies ◽  
Cross Sectional ◽  
Health And Nutrition ◽  
Prevalence Difference ◽  
The Cross ◽  
Us Children

Abstract Data from the cross-sectional National Health and Nutrition Examination Surveys (NHANES) indicate that the seroprevalence of cytomegalovirus immunoglobulin G (IgG) antibodies among US children aged 1–5 years was 20.7% (95% confidence interval [CI]: 14.0, 29.0) in 2011–2012 and 28.2% (95% CI: 23.1–34.0) in 2017–2018 (adjusted prevalence difference, +7.6% [95% CI: −.4, +15.6]).

scholarly journals Immunoglobulin G Antibodies against the Endosymbionts of Filarial Nematodes (Wolbachia) in Patients with Pulmonary Dirofilariasis

2003 ◽  
Vol 10 (1) ◽  
pp. 180-181 ◽  
Author(s):  
F. Simón ◽  
G. Prieto ◽  
R. Morchón ◽  
C. Bazzocchi ◽  
C. Bandi ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Dirofilaria Immitis ◽  
Surface Protein ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
Bacterial Endosymbiont ◽  
Filarial Nematodes ◽  
Pulmonary Dirofilariasis ◽  
Diagnostic Applications

ABSTRACT The dog parasite Dirofilaria immitis can infect humans. Patients with pulmonary dirofilariasis were tested for immunoglobulin G (IgG) antibodies against the surface protein of Wolbachia, the bacterial endosymbiont of D. immitis. These patients showed significantly higher IgG titers than healthy individuals from areas in which D. immitis was endemic as well as areas in which it was not endemic. Titration of anti-Wolbachia surface protein IgG could become useful for diagnostic applications.

scholarly journals Declining Levels of Neutralizing Antibodies Against SARS-CoV-2 in Convalescent COVID-19 Patients One Year Post Symptom Onset

2021 ◽  
Vol 12 ◽  
Author(s):  
Tiandan Xiang ◽  
Boyun Liang ◽  
Yaohui Fang ◽  
Sihong Lu ◽  
Sumeng Li ◽  
...  
Keyword(s):  
Symptom Onset ◽  
Neutralizing Antibodies ◽  
Natural Infection ◽  
Protein S ◽  
Igg Antibodies ◽  
Neutralizing Activity ◽  
Specific Igg ◽  
One Year ◽  
Kinetics Of

Major advances have been made in understanding the dynamics of humoral immunity briefly after the acute coronavirus disease 2019 (COVID-19). However, knowledge concerning long-term kinetics of antibody responses in convalescent patients is limited. During a one-year period post symptom onset, we longitudinally collected 162 samples from 76 patients and quantified IgM and IgG antibodies recognizing the nucleocapsid (N) protein or the receptor binding domain (RBD) of the spike protein (S). After one year, approximately 90% of recovered patients still had detectable SARS-CoV-2-specific IgG antibodies recognizing N and RBD-S. Intriguingly, neutralizing activity was only detectable in ~43% of patients. When neutralization tests against the E484K-mutated variant of concern (VOC) B.1.351 (initially identified in South Africa) were performed among patients who neutralize the original virus, the capacity to neutralize was even further diminished to 22.6% of donors. Despite declining N- and S-specific IgG titers, a considerable fraction of recovered patients had detectable neutralizing activity one year after infection. However, neutralizing capacities, in particular against an E484K-mutated VOC were only detectable in a minority of patients one year after symptomatic COVID-19. Our findings shed light on the kinetics of long-term immune responses after natural SARS-CoV-2 infection and argue for vaccinations of individuals who experienced a natural infection to protect against emerging VOC.

scholarly journals A cluster of measles linked to an imported case, Finland, 2017

2017 ◽  
Vol 22 (33) ◽  
Author(s):  
Elina Seppälä ◽  
Viktor Zöldi ◽  
Sakari Vuorinen ◽  
Satu Murtopuro ◽  
Ulpu Elonsalo ◽  
...  
Keyword(s):  
Public Health ◽  
Vaccination Coverage ◽  
Healthcare Providers ◽  
International Public Health ◽  
Imported Case ◽  
Health Authorities ◽  
Raising Awareness ◽  
Public Health Authorities ◽  
Close Cooperation

One imported and five secondary cases of measles were detected in Finland between June and August 2017. The measles sequences available for five laboratory-confirmed cases were identical and belonged to serotype D8. The large number of potentially exposed Finnish and foreign individuals called for close cooperation of national and international public health authorities and other stakeholders. Raising awareness among healthcare providers and ensuring universally high vaccination coverage is crucial to prevent future clusters and outbreaks.

scholarly journals Evaluation of the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 IgM and IgG antibodies in human plasma

2020 ◽  
Vol 509 ◽  
pp. 79-82 ◽  
Author(s):  
Christian Bundschuh ◽  
Margot Egger ◽  
Kurt Wiesinger ◽  
Christian Gabriel ◽  
Martin Clodi ◽  
...  
Keyword(s):  
Human Plasma ◽  
Igg Antibodies ◽  
Immunosorbent Assays

Immunoglobulin G Antibodies to the Antigens of Lucilia Cuprina in the Sera of Fly-Struck Sheep

1980 ◽  
Vol 33 (1) ◽  
pp. 27 ◽  
Author(s):  
IJ O'Donnell ◽  
PE Green ◽  
JA Connell ◽  
PS Hopkins
Keyword(s):  
Immunosuppressive Therapy ◽  
Immunoglobulin G ◽  
Solid Phase ◽  
Lucilia Cuprina ◽  
Igg Antibodies ◽  
Serum Igg ◽  
Australian Sheep ◽  
Solid Phase Radioimmunoassay

A solid-phase radioimmunoassay was used to demonstrate that sheep with myiasis caused by the larvae of the Australian sheep blowfly, L. cuprina, had serum IgG antibodies to antigens present in an extract of the ground-up larvae. Previously struck animals demonstrated a more severe myiasis than their unstruck counterparts when both groups were subjected to a standard larval challenge. The effects of immunosuppressive therapy were expressed in terms of a decrease in the total number of larvae growing to maturity and in the area of fly strike produced.

scholarly journals Seroprevalence of Immunoglobulin G Antibodies Against Mycobacterium avium subsp. paratuberculosis in Dogs Bred in Japan

2020 ◽  
Vol 7 (3) ◽  
pp. 93
Author(s):  
Takashi Kuribayashi ◽  
Davide Cossu ◽  
Eiichi Momotani
Keyword(s):  
Mycobacterium Avium ◽  
Immunoglobulin G ◽  
Mycobacterium Avium Subsp ◽  
Clinical Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
The Difference ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Veterinary Hospital

In this study, the seroprevalence of immunoglobulin G (IgG) antibodies against Mycobacterium avium subsp. paratuberculosis (MAP) in dogs bred in Japan was evaluated. Ninety-two non-clinical samples were obtained from three institutes and fifty-seven clinical samples were obtained from a veterinary hospital in Japan. Serum titers of total IgG, IgG1 and IgG2 isotype antibodies against MAP were measured using an indirect enzyme-linked immunosorbent assay (ELISA). The IgG antibodies against MAP in non-clinical serum obtained from three institutes was observed to be 2.4%, 20% and 9.0%. Similarly, the IgG1 antibodies titers against MAP were observed to be 7%, 20% and 0%. Lastly, the IgG2 antibodies against MAP were observed to be 7%, 20% and 4.4%. No significance differences in these titers were observed among the three institutes. The IgG, IgG1 and IgG2 antibodies in serum obtained from a veterinary hospital were observed to be 55.3%, 42% and 42%, respectively. Significant differences were found between the non-clinical and clinical samples. The titers in the clinical samples showed a high degree of variance, whereas low variance was found in the non-clinical samples. The IgG antibody levels were thought to be induced following exposure to MAP-contaminated feed. The difference in titers between the clinical and non-clinical samples is likely to be related to the amount of MAP antigen contamination in dog foods.

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