scholarly journals Long-Term Stability of 18 Nutritional Biomarkers Stored at −20 °C and 5 °C for up to 12 Months

2018 ◽  
Vol 3 (1) ◽  
pp. 100-108
Author(s):  
Huiping Chen ◽  
Maya R Sternberg ◽  
Rosemary L Schleicher ◽  
Christine M Pfeiffer
Keyword(s):  
Mixed Model ◽  
Linear Mixed Model ◽  
Iron Status ◽  
Geometric Mean ◽  
Water Soluble ◽  
Reference Condition ◽  
Term Stability ◽  
Long Term Stability ◽  
Long Term Storage

Abstract Background Consistent information on long-term storage stability for a broad range of nutritional biomarkers is lacking. We investigated the stability of 18 biomarkers stored at suboptimal temperatures (−20 °C and 5 °C) for up to 12 months. Methods Multiple vials of serum or whole blood pools (3 concentrations) were stored at −20 °C or 5 °C, removed from the −20 °C freezer after 3, 6, 9, and 12 months and from the 5 °C refrigerator after 6 and 12 months, and placed into a −70 °C freezer until analysis at study completion. Vials stored continuously at −70 °C were used as the reference condition for optimal storage. We measured 18 biomarkers: 4 iron status, 1 inflammation, 8 water-soluble vitamin, and 5 fat-soluble vitamin. For each temperature, we calculated geometric mean concentrations and average percent changes of geometric means across pools relative to the reference condition estimated from a linear mixed model. Results Most biomarkers (13 of 18) showed no difference in concentration after 12 months of storage at −20 °C. Serum ferritin (1.5%), soluble transferrin receptor (−1.7%), and folate (−10.5%) showed small to moderate significant changes at 6 months, but changes were acceptable based on biologic variability. Serum pyridoxal-5′-phosphate (−18.6% at 9 months) and vitamin C (−23% at 6 months) showed large and unacceptable changes at −20 °C. All serum fat-soluble vitamins and iron status indicators, vitamin B12, total homocysteine, and methylmalonic acid showed acceptable changes when stored at 5 °C for up to 12 months. Conclusions Overall, we found good long-term stability for multiple nutritional biomarkers stored at suboptimal temperatures.

Long-term stability of water samples— reanalysis of 20-year-old unpreserved samples

2019 ◽  
Vol 193 (2) ◽  
pp. 167-171
Author(s):  
Espen Enge
Keyword(s):  
Ambient Temperature ◽  
Water Samples ◽  
Water Bodies ◽  
Total Hardness ◽  
Term Stability ◽  
Long Term Stability ◽  
Long Term Storage ◽  
Stability Of Water ◽  
Term Storage

The current study compiles data from the reanalysis of 25 water samples stored unpreserved at ambient temperature for 20 years. Small changes in pH, conductivity and chloride and no significant effects on Al and total hardness were detected. The small effects measured, even during long-term storage under unsuitable conditions, suggest that samples taken from pristine water bodies remain chemically stable over many years.

scholarly journals Combination of Roll Grinding and High-Pressure Homogenization Can Prepare Stable Bicelles for Drug Delivery

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 998 ◽  
Author(s):  
Seira Matsuo ◽  
Kenjirou Higashi ◽  
Kunikazu Moribe ◽  
Shin-ichiro Kimura ◽  
Shigeru Itai ◽  
...  
Keyword(s):  
Drug Delivery ◽  
High Pressure ◽  
Entrapment Efficiency ◽  
Molar Ratio ◽  
High Pressure Homogenization ◽  
Term Stability ◽  
Long Term Stability ◽  
Long Term Storage ◽  
High Entrapment Efficiency

To improve the solubility of the drug nifedipine (NI), NI-encapsulated lipid-based nanoparticles (NI-LNs) have been prepared from neutral hydrogenated soybean phosphatidylcholine and negatively charged dipalmitoylphosphatidylglycerol at a molar ratio of 5/1 using by roll grinding and high-pressure homogenization. The NI-LNs exhibited high entrapment efficiency, long-term stability, and enhanced NI bioavailability. To better understand their structures, cryo transmission electron microscopy and atomic force microscopy were performed in the present study. Imaging from both instruments revealed that the NI-LNs were bicelles. Structures prepared with a different drug (phenytoin) or with phospholipids (dimyristoylphosphatidylcholine, dipalmitoylphosphatidylcholine, and distearoylphosphatidylcholine) were also bicelles. Long-term storage, freeze-drying, and high-pressure homogenization did not affect the structures; however, different lipid ratios, or the presence of cholesterol, did result in liposomes (5/0) or micelles (0/5) with different physicochemical properties and stabilities. Considering the result of long-term stability, standard NI-LN bicelles (5/1) showed the most long-term stabilities, providing a useful preparation method for stable bicelles for drug delivery.

Remarkable Long-term Stability of Cerasome as an Organic–Inorganic Hybrid Nanocontainer for Water-soluble Macromolecules

2011 ◽  
Vol 40 (5) ◽  
pp. 461-463 ◽  
Author(s):  
Takahiro Kawataki ◽  
Kazuma Yasuhara ◽  
Jun-ichi Kikuchi
Keyword(s):  
Water Soluble ◽  
Inorganic Hybrid ◽  
Term Stability ◽  
Long Term Stability

scholarly journals Instability of corticotropin during long-term storage – myth or reality?

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Jacquelien J. Hillebrand ◽  
Li Zhou ◽  
Marilee A. Marcinkus ◽  
Maria Datwyler ◽  
Susan H. Gawel ◽  
...  
Keyword(s):  
Storage Time ◽  
Storage Temperature ◽  
Ethylenediaminetetraacetic Acid ◽  
Venous Blood ◽  
Sampling Studies ◽  
Term Stability ◽  
Long Term Stability ◽  
Long Term Storage ◽  
Term Storage

Abstract Objectives Corticotropin is notorious for its instability. Whereas several studies have investigated its short-term stability in plasma following venous blood sampling, studies on long-term stability are lacking. Here we investigated the long-term storage stability of corticotropin in ethylenediaminetetraacetic acid containing plasma. Methods Specimens from healthy volunteers (neat, spiked) were stored in polypropylene microcentrifuge tubes with socket screw-caps at −20 °C and −70 °C for up to one and a half years. Corticotropin in plasma was measured using an Abbott research only immunoassay. Separately, specimens from patients were collected during diagnostic routine testing and stored in polystyrene tubes with push-caps at −20 °C for up to 6 years. In these samples corticotropin hormone was measured using the Diasorin corticotropin immunoassay. Results Storage of specimens at −20 °C or −70 °C for up to one and a half years showed minimal changes (<11%) in corticotropin levels, while storage of patient samples at −20 °C for up to 6 years showed a significant (54%) reduction in corticotropin levels. Conclusions Corticotropin levels are stable in plasma when stored at −20 °C for one and a half years using the Abbott research only assay, but with longer storage time a significant reduction in corticotropin levels can be expected. Once specimens are stored for future corticotropin measurements, one should consider storage time, storage temperature and assay differences.

Study on Metabolic System for Water-Soluble Coolant — Machining Performance and Long-Term Stability of Recycled Coolant

2009 ◽  
Vol 407-408 ◽  
pp. 313-316 ◽  
Author(s):  
Kenji Yamaguchi ◽  
Yasuo Kondo ◽  
Satoshi Sakamoto ◽  
Shu Kohira
Keyword(s):  
Tap Water ◽  
Water Soluble ◽  
Environmental Load ◽  
Water Recovery ◽  
Recycled Water ◽  
Machining Performance ◽  
Metabolic System ◽  
Term Stability ◽  
Long Term Stability

To reduce the management cost and environmental load of water-soluble coolant, authors have been studying on a metabolic system for water-soluble coolant. More than 90% of waste water-soluble coolant is composed of water phase. If the oily additives and contaminants can be isolated from the waste coolant, the amount of waste coolant decreases remarkably because the recycled water can be reutilized as a diluent of renewal coolant. Authors has been developed some types of water recovery methods for the metabolic system. To complete the metabolic system for water-soluble coolant, the coolant diluted with recovered water should have the same machining performance and long-term stability as those of coolant diluted with tap water. In this report, we examined the machining performance and ability change of water-soluble coolant diluted with recycled water under operations encountered in machine tool.

Long-term stability of biomarkers of the iron status in human serum and plasma

Biomarkers ◽  
2013 ◽  
Vol 18 (4) ◽  
pp. 365-368 ◽  
Author(s):  
Eugène H. J. M. Jansen ◽  
Piet K. Beekhof ◽  
Erna Schenk
Keyword(s):  
Human Serum ◽  
Iron Status ◽  
Term Stability ◽  
Long Term Stability

Long-term stability of organic–inorganic hybrid perovskite solar cells with high efficiency under high humidity conditions

2017 ◽  
Vol 5 (4) ◽  
pp. 1374-1379 ◽  
Author(s):  
Yue Sun ◽  
Yihui Wu ◽  
Xiang Fang ◽  
Linjun Xu ◽  
Zhijie Ma ◽  
...  
Keyword(s):  
Solar Cells ◽  
High Efficiency ◽  
Perovskite Solar Cells ◽  
Cost Effective ◽  
Water Soluble ◽  
Inorganic Hybrid ◽  
Term Stability ◽  
Long Term Stability ◽  
Hybrid Perovskite

Perovskite solar cells with superior tolerance to humidity (85–95% RH) and long-term stability have been achieved via adding a certain amount of a cost-effective and available water soluble additive, polyvinyl alcohol (PVA).

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