Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA ("Cold-Start Method")

2003 ◽  
pp. 285-291
Author(s):  
Michael Lardelli
Keyword(s):  
Cold Start ◽  
Pcr Method ◽  
Suppression Pcr

scholarly journals Development of Microsatellite Markers by ISSR-suppression-PCR Method in Brassica rapa

2005 ◽  
Vol 55 (2) ◽  
pp. 247-252 ◽  
Author(s):  
Koji Tamura ◽  
Miki Nishioka ◽  
Masaki Hayashi ◽  
Zengcui Zhang ◽  
Chunlan Lian ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Brassica Rapa ◽  
Pcr Method ◽  
Suppression Pcr

scholarly journals Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA

BioTechniques ◽  
2000 ◽  
Vol 28 (5) ◽  
pp. 895-902 ◽  
Author(s):  
Richard Tamme ◽  
Esther Camp ◽  
R. Daniel Kortschak ◽  
Michael Lardelli
Keyword(s):  
Pcr Method ◽  
Suppression Pcr

Development of microsatellite markers from an ectomycorrhizal fungus, Tricholoma matsutake, by an ISSR-suppression-PCR method

Mycorrhiza ◽  
2003 ◽  
Vol 13 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Chunlan Lian ◽  
Taizo Hogetsu ◽  
Norihisa Matsushita ◽  
Alexis Guerin-Laguette ◽  
Kazuo Suzuki ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Ectomycorrhizal Fungus ◽  
Tricholoma Matsutake ◽  
Pcr Method ◽  
Suppression Pcr

Investigation of duck plague virus in hoar areas of Bangladesh

2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Clinical Sample ◽  
Chorioallantoic Membrane ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Duck Plague Virus ◽  
Pcr Method ◽  
Polymerase Chain ◽  
The Individual ◽  
Free Ranging

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78

Diagnosis of helicobacter pylori infection: problems and prospects

2020 ◽  
pp. 54-59
Author(s):  
A. S. Molostova ◽  
N. S. Gladyshev ◽  
A. V. Svarval ◽  
R. S. Ferman ◽  
A. B. Karasyova ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Urease Activity ◽  
Diagnostic Methods ◽  
Study Group ◽  
Biochemical Method ◽  
Antimicrobial Drugs ◽  
Non Invasive ◽  
Number Of Patients ◽  
Pcr Method ◽  
Positive Results

(HP) infection was performed using invasive and non-invasive methods. The study group consisted of 95 patients with dyspepsia. HP infection was detected in 47 patients (49.4 %). The expediency of using a set of diagnostic methods for detecting HP (PCR, immunochromatographic, bacteriological and method for determining urease activity) is proved. Most often (100 %) in patients HP infection was detected in biopsies using the PCR method. Somewhat less frequently it was detected when examining biopsies with an invasive biochemical method (AMA RUT Reader) (82 %) and fecal immunochromatographic method (83 %). Despite the fact that helicobacteriosis was detected bacteriologically in a small number of patients (24 %), this method is of particular value, since it allows you to assess the sensitivity to antimicrobial drugs and probiotics, and does not give false positive results.

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