scholarly journals Prevalence, molecular typing, and antimicrobial resistance of bacterial pathogens isolated from ducks

2019 ◽  
Vol 12 (5) ◽  
pp. 677-683 ◽  
Author(s):  
Hamza M. Eid ◽  
Abdelazeem M. Algammal ◽  
Wael K. Elfeil ◽  
Fatma M. Youssef ◽  
Sawsan M. Harb ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Bacterial Pathogens ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
E Coli ◽  
Antibiotic Sensitivity Test ◽  
Amplicon Size ◽  
Spa Gene ◽  
Specific Amplicon ◽  
Apparently Healthy

Aim: This study aimed to investigate the prevalence of different bacterial species affecting ducks as well as demonstrating the antimicrobial susceptibility and molecular typing of the isolated strains. Materials and Methods: A total of 500 samples were randomly collected from different duck farms at Ismailia Governorate, Egypt. The collected samples were subjected to the bacteriological examination. Polymerase chain reaction (PCR) was applied for amplification of Kmt1 gene of Pasteurella multocida and X region of protein-A (spA) gene of the isolated Staphylococcus aureus strains to ensure their virulence. The antibiotic sensitivity test was carried out. Results: The most common pathogens isolated from apparently healthy and diseased ducks were P. multocida (10.4% and 25.2%), Escherichia coli (3.6% and 22.8%), Staphylococcus epidermidis (10% and 8.8%), Pseudomonas aeruginosa (2% and 10%), and Proteus vulgaris (0.8% and 10%), respectively. In addition, S. aureus and Salmonella spp. were isolated only from the diseased ducks with prevalence (12.2%) and (2.8%), respectively. Serotyping of the isolated E. coli strains revealed that 25 E. coli strains were belonged to five different serovars O1, O18, O111, O78, and O26, whereas three strains were untypable. Salmonella serotyping showed that all the isolated strains were Salmonella Typhimurium. PCR revealed that four tested P. multocida strains were positive for Kmt1 gene with specific amplicon size 460 bp, while three strains were negative. In addition, all the tested S. aureus strains were positive for spA gene with specific amplicon size 226 bp. The antibiotic sensitivity test revealed that most of the isolated strains were sensitive to enrofloxacin, norfloxacin, and ciprofloxacin. Conclusion: P. multocida is the most predominant microorganism isolated from apparently healthy and diseased ducks followed by E. coli and Staphylococci. The combination of both phenotypic and genotypic characterization is more reliable an epidemiological tool for identification of bacterial pathogens affecting ducks.

scholarly journals Bacteriological and pathological investigation of goat lungs in Mymensingh and determination of antibiotic sensitivity

2014 ◽  
Vol 11 (2) ◽  
pp. 159-166 ◽  
Author(s):  
MM Rashid ◽  
MJ Ferdoush ◽  
M Dipti ◽  
P Roy ◽  
MM Rahman ◽  
...  
Keyword(s):  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Lung Lesions ◽  
E Coli ◽  
Pathological Conditions ◽  
Hemorrhagic Pneumonia ◽  
Antibiotic Sensitivity Test ◽  
Emphysematous Lung ◽  
Antibiotics Sensitivity

The foremost important goal of the present study was to investigate the bacteriological and pathological conditions in lungs of goats slaughtered in four different slaughter houses/places of Mymensingh Sadar, Mymensingh and in addition to it, antibiotic sensitivity test of commonly used antibiotics in Veterinary practices were performed on isolated bacteria. A total of 75 lungs of slaughtered goats were examined individually and out of which 20 affected lungs were collected for histopathology and bacterial isolation respectively from Mymensingh Sadar, Mymensingh in Bangladesh, during the period from January to May 2013.The lung lesions was grossly recorded 40% in goats (30 out of 75 lungs examined). Grossly, the lung lesions were categorized into (a) hemorrhages 35% (b) congestion 25% (c) hemorrhage and congestion 15% (d) emphysematous lung 15% and (e) hepatization in lung10%. In histopathology, lung lesions were categorized into (a) bronchopneumonia 30%, (b) pneumonia 25% (c) hemorrhagic pneumonia 20% (d) emphysema 15%, (e) purulent pneumonia 10%. Pasteurella sp. (15%) was isolated from the lung lesions of hemorrhagic pneumonia, E. coli. (25%) from bronchopneumonia and Staphylococcus sp. (40%) from purulent pneumonia, focal pneumonia and emphysema, and mixed infection (Staphylococcus sp. and E. coli) 20%. Finally antibiotics sensitivity test was performed on isolated bacteria to which ciprofloxacin was more powerful than others (penicillin, amoxicillin, streptomycin, nalidixic acid and kanamycin) tested and the second one was oxytetracyclin.DOI: http://dx.doi.org/10.3329/bjvm.v11i2.19142Bangl. J. Vet. Med. (2013).11(2): 159-166

A RETROSPECTIVE STUDY ON ANTIBIOTIC USAGE IN A TERTIARY CARE HOSPITAL

2020 ◽  
Vol 4 (6) ◽  
Author(s):  
Anna Joy ◽  
Aparna Anand ◽  
Arathy R Nath ◽  
Meera S Nair ◽  
Dr. K. G. Prasanth
Keyword(s):  
Retrospective Study ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Actual Process ◽  
Care Hospital ◽  
Rational Use ◽  
Antibiotic Susceptibility Test ◽  
Antibiotic Sensitivity Test ◽  
Rational Use Of Antibiotics

Antibiotics are one of the most commonly prescribed drugs today. Rational use of antibiotics is therefore extremely important as their injudicious use can adversely affect the patient. Drug Utilization Evaluation (DUE) is a system of ongoing systematic criteria based evaluation of drug that will help to ensure that medicines are used appropriately. It is drug/disease specific and can be structured so that it will assess the actual process of prescribing, dispensing, or administration of drug. The retrospective study was conducted At Pk Das Institute of Medical Sciences, Palakkad, Kerala for a duration of 6 months (February 2017 - January 2018). A source of data includes Patient case sheets &medication charts, nursing charts, culture & sensitivity reports. The inclusion criterion includes Patients aged between 18- 80 year, prescribed with oral and parenteral antibiotics. . On analyzing the gender, male gender (n= 111, 55.5%) were higher in numbers as compared to female counterparts (n=89, 44.5%). In our study the majority of the patients prescribed with antibiotics were with the clinical assessment of COPD (n=39, 19.5%), UTI (n=37, 18.5%) and LRTI (n=28, 14%), Bronchial asthma (n=19, 9.5%) respectively. On analyzing the data based on antibiotic sensitivity test, antibiotic test were performed and followed in (n=64,32%)prescriptions and in (n=47,23.5%)prescriptions were test is not followed respectively. In (n=89,44.5%) prescriptions, antibiotic sensitivity test is not performed. On analysis of antibiotics prescribed, the most commonly prescribed antibiotics were cephalosporins, of these ceftriaxone was highly prescribed of all (n=95). The high percentage of antibiotic prescriptions may indicate a high probability of irrational use. This study also point out irrational use of antibiotics are more leading to resistance, misuse and serious problems. So certain strategies should be put forward to strengthen rational use of antibiotics. Keywords: Antibiotics, Antibiotic Susceptibility Test, Irrational use, Resistance

scholarly journals Bacteriological profile and antibiotic sensitivity pattern in patients with Urinary tract infection

2017 ◽  
Vol 7 (1) ◽  
pp. 1066-1069 ◽  
Author(s):  
N Subedi ◽  
S Pudasaini
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Urine Culture ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Age Group ◽  
E Coli ◽  
Bacteriological Profile ◽  
Culture And Sensitivity ◽  
Culture Positive

Background: Urinary tract infection is one of the common bacterial infections seeking treatment in clinical practice. A variety of organisms are associated with UTI and the most common organisms are Escherichia coli and other coliforms. Bacteriological investigations of UTI are not complete without antibiotic sensitivity test of the isolate. The aim of this study is to determine the bacteriological profile and antibiotic sensitivity patterns and their disease association.Materials and methods: This study was conducted in Shankarapur Hospital over a period of one year. All cases of suspected UTI sent for urine culture and sensitivity test were evaluated in this study. Disease associated with UTI, bacteriological profile and antibiotic sensitivity patterns were evaluated.Results: A total of 974 cases were sent for urine culture and sensitivity test. The total culture positive cases were 165 (17.4%).  The most common age group for culture positive test was 21- 30 years (33.3%) followed by 31- 40 years (25.5%). Female patients formed the majority of culture positive cases (84.8%) and E Coli (86.1%) was the most common organism isolated. Piperacillin- tazobactum and ceftriaxone were the most common antibiotic sensitive to the organisms. Simple uncomplicated UTI and PID were the most common indication for subjecting the patients to urine culture.Conclusion: UTI is most commonly seen in female of reproductive age group and the most common causative organism is E coli. Culture result and antibiogram helps the clinician for specific treatment of UTI.

scholarly journals BACTERIOLOGICAL EXAMINATION OF PUS SAMPLE COLLECTED FROM A COW: A CASE STUDY

2016 ◽  
Vol 4 (12) ◽  
pp. 31-33
Author(s):  
Subha Ganguly
Keyword(s):  
Present Article ◽  
Antibiotic Sensitivity ◽  
Neck Region ◽  
Sensitivity Test ◽  
Bacteriological Examination ◽  
Antibiotic Sensitivity Test

The present article reports on the bacteriological examination and antibiotic sensitivity test of pus sample collected from an abscess at the neck region of a cow.

scholarly journals Study of ELISA and antibiotic sensitivity test for Salmonella enteritidis as experimental infection in mice

2009 ◽  
Vol 6 (1) ◽  
pp. 114-122
Author(s):  
Baghdad Science Journal
Keyword(s):  
Salmonella Enteritidis ◽  
Antibiotic Sensitivity ◽  
Immunological Response ◽  
Elisa Test ◽  
Sensitivity Test ◽  
Test Results ◽  
Antibiotic Sensitivity Test ◽  
Bacterial Sensitivity ◽  
Antibiotics Sensitivity ◽  
Tract Infections

Salmonella enteritidis one of more important as epidemiological bacteria between other salmonella types. It is very important pathologically that cause food poising and gastrointestinal tract infections. This study includes some of immunological changes that appear by ELISA test and antibiotic sensitivity test against these bacteria in mice. ELISA test results appears high immunological response happen after 3 days of inoculation, mean titration readings beginning 0.198 and the maximum mean titration after 15 days of inoculation 1.538 and begin to decrease after this time slowly to remain about 0.297 after 40 days of inoculation. An antibiotics sensitivity test result appears, this bacteria sensitive to Chloramphenicol, Ceftriaxone, Ciprofloxacin and Cotrimaxazol. Resistance to Neomycin, Streptomycin and Rifampicin, while intermediate against Ampicilin and Amoxicillin. Another test we use Vitek system to know bacterial sensitivity against to more another types of antibiotics and to confirm between some of them.

scholarly journals ISOLATION AND IDENTIFICATION OF MICROFLORA FROM APPARENTLY HEALTHY CAGED PARROTS OF DHAKA ZOO OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 05-10 ◽  
Author(s):  
J Akhter ◽  
MT Hossain ◽  
MT Islam ◽  
MP Siddique ◽  
MA Islam
Keyword(s):  
Research Work ◽  
Antibiotic Sensitivity ◽  
Biochemical Properties ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Gram Negative ◽  
E Coli ◽  
Apparently Healthy

The research work was conducted to isolate and identify the microflora from apparently healthy caged parrots. A total of 45 samples (oral swabs, cloacal swabs and feces) were collected from five types of caged parrots (Gray cockatiels, Rose ringed parakeet, Alexandriane parakeet, Red breast parakeet and Blossom headed parakeet) of Dhaka Zoo during the period from April to August 2009. The samples were cultured on different bacteriological media and the bacteria were identified by their cultural and biochemical properties. All the isolates were allowed for antibiogram study. The bacteria isolated in this study from different types of caged parrots were E. coli (64.44%), Salmonella spp. (46.67%), Staphylococcus spp. (46.67%), Pasteurella spp. (33.33%), Proteus spp. (6.67%) and some unidentified Gram-positive and Gram-negative bacteria. Of these isolates, E. coli was the most frequent isolate. The frequency of Gram-negative bacteria was higher in this study. The percentage of bacterial isolates recovered from each type of parrots was almost similar. Irrespective of types of parrots, the higher percentage of different bacteria was isolated from cloacal swab (77.78%) followed by feces (75.56%). The 68.89% isolates were recovered from oral swab. All the suspected isolates of Salmonella spp. were confirmed by slide agglutination test using Salmonella polyvalent ‘O’ antiserum. Among the 21 Salmonella spp. isolated in this study, 4 (19.05%) isolates were identified as S. Pullorum when tested with specific antisera against S. Pullorum. The results of antibiotic sensitivity tests revealed that ampicillin and amoxicillin were completely resistant to E. coli and Pasteurella spp.; ampicillin to Proteus spp.; and furazolidone to Salmonella spp. and Pasteurella spp. However, the antibiotics of fluoroquinolone group such as ciprofloxacin, norfloxacin and enrofloxacin showed moderate to high sensitivity against almost all the bacterial isolates. Of these, ciprofloxacin was found to be consistently highly sensitive to all the bacterial isolates. DOI = 10.3329/bjvm.v8i1.8349 Bangl. J. Vet. Med. (2010). 8(1): 05-10

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