Leukocyte populations and their cell adhesion molecules expression in newborn dromedary camel calves

Background and Aim: Different properties of the newborn immune system have been characterized in many species. For the newborn camel calf, however, the phenotype and composition of blood leukocytes have so far not been evaluated. The current study aimed to analyze the distribution of leukocyte subpopulations and their expression pattern of cell adhesion molecules in newborn and adult dromedary camels. Materials and Methods: Blood samples were collected from 17 newborn camel calves and 32 adult camels. For each sample, total leukocytes were separated and analyzed for their composition and cell adhesion molecules expression by flow cytometry. Results: In comparison to adult camels, newborn camel calves had higher leukocyte numbers and higher numbers of neutrophils, monocytes, and lymphocytes but lower numbers of eosinophils in their blood. Among the lymphocyte populations in calves, the fractions of B cells and γδ T cells were elevated when compared to adults, whereas CD4-positive T cells were reduced. The comparison between camel calves and adult camels revealed significantly lower expression of the cell adhesion molecules CD11a, CD11b, and CD18 on granulocytes, monocytes, and lymphocytes in calves. Conclusion: Newborn camel calves show a distinct composition and phenotype pattern of blood leukocytes when compared to adult camels. The observed rise in many leukocyte populations in calf blood may be due to reduced migratory activity in calf leukocyte populations.

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Expression Patterns of Cell Adhesion Molecules on CD4+ T Cells and WC1+ T Cells in the Peripheral Blood of Dromedary Camels

Pakistan Veterinary Journal ◽

10.29261/pakvetj/2018.055 ◽

2018 ◽

Vol 38 (03) ◽

pp. 231-236 ◽

Cited By ~ 1

Author(s):

Jamal Hussen

Keyword(s):

T Cells ◽

Cell Adhesion ◽

Adhesion Molecules ◽

Peripheral Blood ◽

Cd4 T Cells ◽

Cell Adhesion Molecules ◽

Expression Patterns ◽

Dromedary Camels

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Expansion of T cells negative for CD28 expression in hiv infection. Relation to activation markers and cell adhesion molecules, and correlation with prognostic markers

Medical Microbiology and Immunology ◽

10.1007/s004300050010 ◽

1996 ◽

Vol 185 (1) ◽

pp. 19-25 ◽

Cited By ~ 26

Author(s):

R. Kämmerer ◽

A. Iten ◽

P. C. Frei ◽

P. Bürgisser

Keyword(s):

T Cells ◽

Cell Adhesion ◽

Hiv Infection ◽

Adhesion Molecules ◽

Cell Adhesion Molecules ◽

Prognostic Markers ◽

Activation Markers

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Immunization with a Mimotope of GD2 Ganglioside Induces CD8+T Cells That Recognize Cell Adhesion Molecules on Tumor Cells

The Journal of Immunology ◽

10.4049/jimmunol.181.9.6644 ◽

2008 ◽

Vol 181 (9) ◽

pp. 6644-6653 ◽

Cited By ~ 23

Author(s):

Andrzej Wierzbicki ◽

Margaret Gil ◽

Michael Ciesielski ◽

Robert A. Fenstermaker ◽

Yutaro Kaneko ◽

...

Keyword(s):

T Cells ◽

Cell Adhesion ◽

Tumor Cells ◽

Adhesion Molecules ◽

Cd8 T Cells ◽

Cell Adhesion Molecules ◽

Gd2 Ganglioside

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Reduced frequency of perforin-positive CD8+ T cells in menstrual effluent of endometriosis patients compared to healthy controls

10.1101/2020.12.03.20243436 ◽

2020 ◽

Author(s):

Timo Schmitz ◽

Verena Hoffmann ◽

Elisabeth Olliges ◽

Alina Bobinger ◽

Roxana Popovici ◽

...

Keyword(s):

T Cells ◽

Cell Adhesion ◽

Nk Cells ◽

Adhesion Molecules ◽

Cd8 T Cells ◽

Cell Adhesion Molecules ◽

Nk Cell ◽

Control Group ◽

Healthy Controls ◽

Tnf Α

AbstractBackgroundEndometriosis is a widespread disease in women of reproductive age. The disease often reduces life quality of women affected by symptoms like dysmenorrhea, dyspareunia or infertility. Diagnosis remains challenging and no causal treatments exist until now. The scientific literature indicates many immunological changes like reduced cytotoxicity of natural killer cells and macrophages or altered concentrations of cytokines or cell adhesion molecules in women with endometriosis. Research has been concentrated on immunological alterations in peripheral blood, endometrial tissue and peritoneal fluid of women with endometriosis. Yet, knowledge on immunological differences in menstrual effluent is scarce. The aim of this study was to investigate menstrual effluent of hormone-free women with endometriosis in comparison to age matched healthy controls regarding selected immunological parameters.Methods12 women with endometriosis and 11 healthy controls were included in the study. Menstrual effluent (ME) was collected using menstrual cups over a time period of 24 h, and venous blood samples (PB) were taken. Mononuclear cells were obtained from ME (MMC) and PB (PBMC) using density gradient centrifugation and analyzed using flow cytometry. Furthermore, concentrations of cell adhesion molecules (ICAM-I and VCAM-I) and cytokines (IL-6, IL-8 and TNF-α) were measured in centrifugation supernatant of ME and plasma of PB.ResultsThe comparison of MMC from women with endometriosis and healthy controls revealed a significantly lower frequency of perforin-positive cells among CD8+ T cells in endometriosis patients compared to healthy controls. No additional differences regarding frequencies of CD8+ or CD4+ T cells, regulatory T cells (Tregs), NK cell subsets or monocytic subsets could be detected between MMC or PBMC, of endometriosis patients and healthy controls. Comparing MMC with PBMC revealed that MMC contained significantly more B cells and significantly less T cells than PBMC. Among the T cells, the CD4/CD8 ratio was significantly higher in MMC, and Tregs were significantly less common in MMC. T cells and NK cells expressed significantly more CD69 in MMC., NK cells of the MMC were predominantly CD56bright/CD16dim and the CD56dim NK cell subset had a low frequency of perforin+ cells, in contrast to CD56dim NK cells of PBMC, which were predominantly perforin-positive. However, NKp46 was significantly more expressed on NK cells from MMC. Finally, the endometriosis group had significantly lower plasma ICAM-I concentrations than the control group. There were no significant differences in VCAM-1, IL-6, IL-8 or TNF-α between the two groups.ConclusionMMC are distinctively different from PBMC and, thus, seem to be of endometrial origin. The CD8+ T cells from the ME were significantly less perforin-positive in endometriosis patients than in healthy controls, suggesting a reduced cytotoxic potential.

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