scholarly journals Fractionation of Sugarcane Extracts and Determination of Phenolic Compounds and their Antioxidant Activity

2022 ◽  
Vol 34 (2) ◽  
pp. 284-288
Author(s):  
K. Kerdchan ◽  
P. Srihanam
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
High Performance ◽  
Hplc Analysis ◽  
Saccharum Officinarum ◽  
Phenolic Substance ◽  
Ethanolic Extracts ◽  
The Individual ◽  
Phytochemical Contents

Crude ethanolic extracts of four cultivars of sugarcane (Saccharum officinarum L.) were firstly prepared and then fractionated using silica gel column chromatography before the determination of their oxidative substances. The fractionated extracts were then tested for antioxidant activity by various assays. Finally, high-performance liquid chromatography (HPLC) was applied for the quantitative determination of the individual phenolic compounds. Sub-fraction 2 has the highest phytochemical contents as well as antioxidant activity. All tested phytochemicals had positively correlated to antioxidant activity. HPLC analysis showed that the phytochemicals in the fractionated extracts varied by the sugarcane cultivars. The main flavonoid substances found in the fractionated extracts were epicatechin, catechin, quercetin, resveratrol, myricetin and rutin while gallic acid was the main phenolic substance. The obtained information is useful for further studies and applications.

Comparative Study on Two Variants of Laghupanchamula (A Compound Ayurvedic Formulation) for Important Groups of Phytochemicals and Antioxidant Activity

2017 ◽  
Vol 2 (3) ◽  
pp. 157-163
Author(s):  
Shivani Ghildiyal ◽  
Apurva Joshi ◽  
Vinod K Joshi
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
High Performance ◽  
Hplc Analysis ◽  
Shikimic Acid ◽  
Ricinus Communis ◽  
Chemical Constituents ◽  
Tribulus Terrestris

ABSTRACT Introduction Laghupanchamula is a compound formulation prepared by combinations of roots of five herbs. Two variants of Laghupanchamula have been described in Ayurvedic classics where beside four common herbs fifth one is either Gokshura (Tribulus terrestris L.) or Eranda (Ricinus communis L.). The objective of the study is to make comparison between two variants of Laghupanchmula with respect to important group of phytochemicals and antioxidant activity to corroborate the science behind their therapeutic utility. Materials and methods Standard methods have been followed for quantitative determination of total quantity of phenols, tannins, flavonoids, and flavonols, and in vitro antioxidant activity in variants of Laghupanchmula formulations. Qualitative high-performance liquid chromatography (HPLC) analysis has also been performed to establish presence/absence of important chemical constituents in formulations. Results Additional quantity of phenols, tannins, flavonoids, and flavonols has been observed in Laghupanchamula variant containing Eranda than the variant containing Gokshura. Greater antioxidant activity has also been found in formulation containing Eranda. The HPLC analysis revealed the presence of shikimic acid, gallic acid, catechin in both formulations, but rutin has been found only in formulation containing Eranda. Conclusion From the results of experiments it has been observed that the formulation containing Eranda has more antioxidant activity as it contains more quantities of phenol, tannins, flavonoids, and flavonols than the formulation containing Gokshura. How to cite this article Ghildiyal S, Joshi A, Joshi VK. Comparative Study on Two Variants of Laghupanchamula (A Compound Ayurvedic Formulation) for Important Groups of Phytochemicals and Antioxidant Activity. J Drug Res Ayurvedic Sci 2017;2(3):157-163.

scholarly journals Determination of Quality Characteristics, Phenolic Compounds and Antioxidant Activity of Propolis from Southeastern Mexico

2021 ◽  
Vol 65 (1) ◽  
pp. 109-122
Author(s):  
Enrique Sauri-Duch ◽  
Cesia Gutiérrez-Canul ◽  
Luis F. Cuevas-Glory ◽  
Lorena Ramón-Canul ◽  
Emilio Pérez-Pacheco ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Geographical Location ◽  
Principal Component ◽  
Total Phenolic ◽  
Dry Extract ◽  
Extract Content ◽  
The Individual

Abstract The objective of this work was to investigate the variability of physicochemical parameters, phenolic compounds and in vitro antioxidant activity of propolis collected from different apiaries in southeastern Mexico. A high variability was found in the moisture content (1.96–8.26%), ash (0.66–5.50%) and sensory characteristics of raw propolis from southeastern Mexico, but the raw propolis samples met the requirements of the quality regulations. In the same way, most of the ethanolic extracts also complied with the quality regulations. Of all the extracts, PE2 obtained from Santa Cruz showed the highest values for dry extract, content of total phenolic compounds (TPC), total flavonoids (TF) and antioxidant activity (DPPH and ABTS). The content of the individual phenolic compounds varied according to the geographical location of the apiary, but the PE2 extract resulted in the highest pinocembrin and chrysin content. A positive correlation was obtained between TPC and TF with antioxidant activity. Propolis extracts were classified into two groups through principal component analysis (PCA). These results indicate that the apiary location in southeastern Mexico influenced the characteristics of propolis.

Polyphenol Composition and Antioxidant Activity of Andrographis paniculata L. Nees

2017 ◽  
Vol 13 (4) ◽  
pp. 33-46 ◽  
Author(s):  
Praveen N
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
High Performance ◽  
Reducing Power ◽  
High Performance Liquid Chromatographic ◽  
Andrographis Paniculata ◽  
Total Phenolic ◽  
Butanol Extract ◽  
The Individual

The aim of the present study was to establish the polyphenolic profile and antioxidant activity of the leaves of Andrographis paniculata (kalmegh) that could be potentially used for the benefit of mankind. An ultra high performance liquid chromatographic method was used to identify and quantify the individual phenolic compounds of the kalmegh leaves. A total of 20 polyphenolic compounds were identified and quantified in kalmegh leaves, including hydroxybenzoic acids, hydroxycinnamic acids, flavonols and other group of phenolic compounds. The UPLC analysis of the phenolic compounds revealed that salicylic acid was the dominant phenolic compound present in the leaves extract followed by gallic acid, chlorogenic acid, veratric acid and protocatechuic acid. The kalmegh leaves were extracted with 4 different solvents (ethyl acetate, methanol, butanol and water) and screened for total phenolic content (TPC) and antioxidant activity. The antioxidant activity of kalmegh was assessed by evaluating the 1, 1-diphenyl-2-picrylhydrazyl (DPPH), reducing power and phosphomolybdenum assay. Ethyl acetate and butanol extract exhibited the highest phenolic

scholarly journals Determination of Antioxidant Activity of Selected Vegetables Grown in Iran under Reversed Phase Conditions with UPLC

2015 ◽  
Vol 9 (5) ◽  
pp. 122-125
Author(s):  
Nahid Rastakhiz ◽  
Fereshteh Khosravi ◽  
Seyyed Sina Seyyed Jafari Olia ◽  
Behzad Iranmanesh
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Reversed Phase ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Reverse Phase

This study describes the determination of some phenolic compounds in four different vegetables of Kerman in Iran including Carrots, Celery, Lettuce and red cabbage .The  phenolic compounds analyzed were (Ascorbate, Ferulic acid, Naringin) using reverse phase high performance liquid chromatography (RPUPLC). The results of analysis showed in vegetables ranged between 90 and 1080mg /kg. A high and significant correlation between antioxidant activity and total phenolic content was determined in vegetables (r2 = 0.9461 P < 0.06). However, flavonoid content was not significantly correlated with antioxidant activity in vegetables. It was observed that total phenolic content is the major contributor to the antioxidant activity of vegetables.DOI: http://dx.doi.org/10.3126/ijls.v9i5.12712

Determination of Total Phenolic Compounds and Antioxidant Activity of Ethanolic Extracts of Propolis Using ATR–FT-IR Spectroscopy and Chemometrics

2018 ◽  
Vol 11 (7) ◽  
pp. 2013-2021 ◽  
Author(s):  
Cleidiane da Silva ◽  
Anaclara Prasniewski ◽  
Matheus A. Calegari ◽  
Vanderlei Aparecido de Lima ◽  
Tatiane L. C. Oldoni
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ir Spectroscopy ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Ethanolic Extracts ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

scholarly journals Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 409
Author(s):  
Ning-Hui Ma ◽  
Jing Guo ◽  
Si-Han Xu Chen ◽  
Xiu-Rong Yuan ◽  
Tong Zhang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Hplc Analysis ◽  
Hplc Method ◽  
Antioxidant Compounds ◽  
Dpph Assay ◽  
Bamboo Leaves ◽  
Phyllostachys Nigra ◽  
Further Development

Bamboo leaves of Phyllostachys nigra (PN), Lophatherum gracile (LG), and Pleioblastus amarus (PA) are three common herbs in China. In this work, a new high performance liquid chromatography (HPLC) method for the simultaneous determination of seven compounds in bamboo leaves has been developed; and PN, LG, and PA leaves were analyzed. PN showed four times as much chlorogenic acid (CA) than the other two, and contained the most isoorientin (iso-ORI) and isovitexin (iso-VIT) as well. The PA presented the most orientin (ORI) and LG covered a majority of cynaroside (CYN). We measured the antioxidant activity by scavenging the stable 2,2-diphenyl-1-pyridinohydrazinyl (DPPH) free radicals, and found that Luteolin (inhibitory concentration (IC)50 = 0.42 µM, LUT) and CYN (IC50 = 0.43 µM) showed 2–3 times higher antioxidant activity than iso-ORI (IC50 = 0.81 µM), ORI (IC50 = 0.84 µM), and other related antioxidant standards such as trolox (IC50 = 0.97 µM) and ascorbic acid (IC50 = 0.93 µM, VC). Among extracts, PN and PA showed considerable antioxidant activity, which was related well with the contents of CA, iso-ORI, and iso-VIT (p < 0.05). This study firstly provides evidence for functional antioxidant compounds of bamboo leaves based on statistical analysis of the HPLC analysis and DPPH assay, and it lays a foundation for its further development or utilization.

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